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Brsk1 (NM_001127337) Rat Tagged ORF Clone
CAT＃: RR210211
- TrueORF®
Brsk1 (Myc-DDK-tagged ORF) - Rat BR serine/threonine kinase 1 (Brsk1), (10 ug)
"NM_001127337" in other vectors (3)
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CNY 9,980.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RGD1563268; Sad-b |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR210211 representing NM_001127337
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTCCGGGTCCAAGGAAGGTGGCGGGGGCTCCCCCGCCTACCACCTCCCACACCCACACCCACACC CACCCCAGCACGCCCAATATGTGGGCCCCTATCGGCTGGAGAAGACGCTGGGCAAAGGACAGACAGGTCT AGTTAAACTTGGGGTCCACTGCATCACGGGTCAGAAGGTCGCTGTCAAGATCGTGAACAGGGAGAAGCTG TCGGAATCTGTGCTGATGAAGGTGGAGAGGGAAATCGCCATTCTAAAGCTCATTGAACACCCGCATGTGC TCAAGCTCCACGACGTCTACGAGAACAAGAAATATTTATACTTGGTGCTTGAGCATGTTTCTGGTGGTGA GCTGTTCGACTACCTGGTAAAAAAAGGGAGACTGACACCCAAGGAGGCCCGAAAGTTCTTCCGCCAGATT GTGTCAGCGCTGGACTTCTGCCATAGCTACTCCATCTGTCACAGGGACCTGAAGCCAGAGAACCTGCTGC TGGATGAGAAAAACAACATCCGCATCGCAGACTTTGGCATGGCGTCCCTGCAGGTGGGGGACAGCCTCCT GGAGACCAGCTGTGGGTCCCCCCATTACGCATGTCCAGAGGTGATCAAGGGGGAGAAGTATGATGGCCGC CGGGCAGACATGTGGAGCTGTGGAGTCATCCTGTTTGCCCTGCTTGTGGGGGCGCTGCCCTTCGATGATG ACAACCTACGACAGCTACTAGAGAAGGTGAAACGTGGTGTCTTCCACATGCCGCACTTCATCCCTCCAGA CTGCCAGAGCCTCCTGAGAGGGATGATTGAAGTGGAGCCCGAGAAAAGGCTCAGTCTGGAGCAAATTCAG AAACATCCTTGGTATCTGGGCGGGAAACACGAACCAGACCCTTGCCTGGAGCCAGCCCCAGGCCGCAGAG TAGCTATGCGGAGCCTGCCTTCCAATGGCGAGCTGGACCCTGACGTTCTGGAAAGCATGGCGTCTCTGGG CTGCTTCAGAGACCGCGAGCGGCTACACAGAGAACTGCGAAGTGAGGAGGAAAACCAAGAAAAGATGATC TATTATTTGCTTTTGGATCGGAAAGAGCGGTACCCTAGCTGTGAAGACCAGGACCTGCCTCCTCGGAATG ATGTTGACCCACCTCGGAAGCGTGTGGATTCCCCCATGCTGAGCAGACACGGGAAACGGAGGCCAGAGCG GAAGTCCATGGAAGTCCTCAGCATCACAGATGCTGGGAGTGGTGGCTCTCCAGTGCCTACCAGAAGGGCC TTGGAGATGGCCCAGCACAGTCAGAGATCCCGCAGCGTCAGTGGAGCATCCACTGGTCTGTCCTCCAGCC CTCTAAGCAGCCCAAGGAGTCCGGTCTTTTCCTTCTCACCGGAGCCAGGCGTTGGAGATGAGGCTAGAGG TGGAGGCTCCCCAACTTCCAAAACACAGACGCTGCCTTCTCGGGGCCCCAGAGGTGGGGGCGCTGGAGAG CAGCCACCACCTCCCAGTGCCCGTTCCACGCCCTTGCCTGGACCACCAGGCTCCCCGCGCTCCTCCGGCG GAACCCCCTTGCACTCACCTCTGCACACGCCTCGAGCCAGCCCTACCGGGACTCCAGGAACTACACCACC TCCCAGCCCAGGCGGTGGCGTCGGGGGAGCCGCCTGGAGAAGTCGTCTCAACTCCATCCGCAACAGCTTC CTGGGCTCCCCTCGCTTCCACCGGCGCAAGATGCAGGTCCCTACCGCTGAGGAGATGTCCAGTTTGACAC CAGAATCCTCTCCAGAGCTGGCAAAACGCTCCTGGTTCGGGAATTTCATCTCCTTGGACAAAGAAGAACA AATATTCCTCGTGCTAAAGGACAAACCTCTCAGCAGCATCAAAGCGGACATTGTCCATGCCTTTCTGTCG ATCCCTAGCCTGAGTCACAGTGTGCTGTCACAGACCAGCTTCAGGGCCGAATACAAGGCCAGCGGTGGCC CCTCTGTCTTCCAGAAGCCTGTCCGCTTCCAGGTGGACATCAGCTCCTCTGAGGGTCCAGAACCCTCACC CCGAAGAGATGGTAGCAGTGGAGGTGGCATCTACTCAGTCACCTTCACTCTCATCTCCGGTCCTAGCCGT CGGTTCAAGCGTGTGGTAGAGACCATCCAGGCACAGTTGCTGAGTACTCATGACCAGCCCTCCGTGCAGG CCTTGGCAGATGAGAAGAATGGAGCCCAGACCCGGCCTGCTGGTACCCCACCCCGAAGCCTGCAGCCTCC ACCAGGCCGCCCTGACCCTGATCTGAGTAGCTCTCCCCGCCGAGGACCCTCTAAGGACAAGAAGCTCCTG GCCACCAATGGAACCCCTCTACCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR210211 representing NM_001127337
Red=Cloning site Green=Tags(s) MSSGSKEGGGGSPAYHLPHPHPHPPQHAQYVGPYRLEKTLGKGQTGLVKLGVHCITGQKVAVKIVNREKL SESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQI VSALDFCHSYSICHRDLKPENLLLDEKNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIKGEKYDGR RADMWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVEPEKRLSLEQIQ KHPWYLGGKHEPDPCLEPAPGRRVAMRSLPSNGELDPDVLESMASLGCFRDRERLHRELRSEEENQEKMI YYLLLDRKERYPSCEDQDLPPRNDVDPPRKRVDSPMLSRHGKRRPERKSMEVLSITDAGSGGSPVPTRRA LEMAQHSQRSRSVSGASTGLSSSPLSSPRSPVFSFSPEPGVGDEARGGGSPTSKTQTLPSRGPRGGGAGE QPPPPSARSTPLPGPPGSPRSSGGTPLHSPLHTPRASPTGTPGTTPPPSPGGGVGGAAWRSRLNSIRNSF LGSPRFHRRKMQVPTAEEMSSLTPESSPELAKRSWFGNFISLDKEEQIFLVLKDKPLSSIKADIVHAFLS IPSLSHSVLSQTSFRAEYKASGGPSVFQKPVRFQVDISSSEGPEPSPRRDGSSGGGIYSVTFTLISGPSR RFKRVVETIQAQLLSTHDQPSVQALADEKNGAQTRPAGTPPRSLQPPPGRPDPDLSSSPRRGPSKDKKLL ATNGTPLP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001127337 |
| ORF Size | 2334 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001127337.1, NP_001120809.1 |
| RefSeq Size | 2442 bp |
| RefSeq ORF | 2337 bp |
| Locus ID | 499073 |
| UniProt ID | B2DD29 |
| MW | 85.2 kDa |
| Gene Summary | Serine/threonine-protein kinase that plays a key role in polarization of neurons and centrosome duplication. Phosphorylates CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-523' and 'Ser-573'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. Also acts as a positive regulator of centrosome duplication by mediating phosphorylation of gamma-tubulin (TUBG1 and TUBG2) at 'Ser-131', leading to translocation of gamma-tubulin and its associated proteins to the centrosome. Involved in the UV-induced DNA damage checkpoint response, probably by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C (By similarity). In neurons, localizes to synaptic vesicles and plays a role in neurotransmitter release, possibly by phosphorylating RIMS1.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN210211 | Brsk1 (untagged ORF) - Rat BR serine/threonine kinase 1 (Brsk1), (10 ug) |
CNY 9,980.00 |
|
| RR210211L3 | Lenti ORF clone of Brsk1 (Myc-DDK-tagged ORF) - Rat BR serine/threonine kinase 1 (Brsk1), (10 ug) |
CNY 11,880.00 |
|
| RR210211L4 | Lenti ORF clone of Brsk1 (mGFP-tagged ORF) - Rat BR serine/threonine kinase 1 (Brsk1), (10 ug) |
CNY 13,020.00 |
