Ptprt (NM_001108603) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR210452 representing NM_001108603 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGACCCAGCTGTGCCCACAGGGTCCTTCATGATGGTGAATAGCTCTGGAAGAGCTTCAGGCCAGA AAGCCCATCTTCTTCTACCAACCTTGAAGGAGAATGATACCCACTGCATTGACTTCCATTACTACTTCTC CAGCCGAGATCGCTCCAGTCCGGGAGCCTTGAATGTCTACGTGAAGGTGAATGGTGGTCCCCAAGGGAAC CCTGTCTGGAACGTGTCTGGGGTCGTCACTGAGGGCTGGGTGAAGGCAGAACTTGCCATCAGTACCTTCT GGCCTCATTTCTACCAGGTGATATTTGAATCCGTCTCTTTGAAAGGTCATCCTGGTTACATCGCTGTGGA CGAAGTTCGAGTCCTTGCTCATCCATGCAGAAAAGCACCTCATTTCCTGCGACTCCAAAATGTTGAGGTG AATGTGGGGCAGAACGCCACGTTTCAGTGCATTGCCGGTGGGAAGTGGTCCCAGCATGACAAACTTTGGC TCCAGCAATGGAATGGCAGAGACACGGCCCTCATGGTCACCCGGGTGGTCAACCACAGGCGCTTCTCAGC CACAGTGAGTGTGGCAGACACCTCTCAACGCAGTGTCTCCAAGTATCGCTGTGTGATCCGCTCAGACGGT GGGTCTGGCGTATCCAACTATGCAGAGCTGATCGTGAAAGAGCCTCCCACACCTATTGCTCCCCCAGAAC TACTGGCAGTGGGGGCCACCTACCTGTGGATTAAACCAAACGCCAACTCTATTATTGGGGACGGCCCCAT CATTCTGAAGGAGGTAGAATACCGCACGACCACAGGCACTTGGGCTGAGACCCACATCGTGGACTCTCCC AACTACAAGCTCTGGCATCTGGACCCCGATGTGGAGTACGAGATCCGGGTGCTGCTCACACGACCAGGAG AGGGGGGCACAGGACCACCAGGACCTCCCCTCACTACCAGGACCAAGTGTGCCGTTCCAGGAGCTGTTCC TCTGGAGTCCATCCAAGGAGGACCCTTTGAGGAGAAGATCTATATCCAGTGGAAACCTCCCAATGAGACC AATGGGGTCATCACACTCTATGAGATTAATTACAAGGCTGTTGGCTCACTGGATCCAAGTGCTGACCTGT CCAGCCAGAGGGGGAAAGTGTTCAAACTCCGGAATGAAACCCATCACCTCTTTGTGGGTCTGTACCCTGG GACTACCTACTCTTTCACCATCAAGGCCAGCACAGCCAAGGGCTTTGGACCCCCAGTCACCACTCGGATT GCCACCAAGATTTCAGCTCCATCAATGCCCGAATATGATGCAGATAGCCCACTCAATGAAACAGATACGA CCATCACAGTGATGCTCAAACCTGCCCAGTCCCGGGGAGCCCCTGTCAGCGTTTACCAGTTGGTTGTCAA GGAGGAGCGACTTCAGAAGTCTCGGAGAGCAGCTGACATCATCGAGTGTTTCTCAGTGCCCGTGAGCTAC CGGAATGCCTCCAACCTCGATTCTCTACACTACTTTGCTGCTGAGCTGAAGCCCTCCAATCTGCCTGTCA CCCAGCCCTTCACAGTGGGAGACAACAAGACCTACAATGGCTACTGGAATCCACCCCTCTCCCCATTAAA GAGCTACAGCATCTACTTCCAGGCTCTCAGCAAAGCAAATGGAGAGACAAAAATCAACTGCGTGCGTCTG GCTACAAAAGGTGCTTCCACCCAGAATTCTAACACTGTGGAGCCCGAGAAGCAGGTCGACAACACAGTGA AGATGGCTGGTGTGATTGCTGGCCTCCTCATGTTCATCATAATTCTACTGGGGGTGATGCTGACCATCAA AAGGAGGAAGCTGGCTAAGAAGCAGAAGGAGACCCAGAGTGGAGCCCAGAGGGAGATGGGGCCTGTGGCC TCGACTGACAAACCTACCACCAAGCTCAGCACCAACCGCAACGATGAAGGCTTCTCCTCCAGCTCTCAGG ATGTTAACGGATTCACAGATGGCAGCCGTGGAGAGCTGTCTCAGCCCACCCTCACCATTCAGACTCACCC CTACCGGACTTGCGACCCTGTAGAGATGAGCTACCCCCGAGACCAGTTCCAGCCCGCCATCCGGGTAGCA GACCTGCTTCAACACATCACCCAGATGAAGAGAGGCCAGGGCTACGGGTTCAAGGAAGAATATGAGGCCT TACCAGAGGGACAGACAGCTTCGTGGGACACAGCCAAGGAAGATGAAAACCGCAATAAGAATCGATATGG GAACATCATATCTTATGACCACTCCCGAGTGAGGCTGTTGGTGCTGGATGGAGACCCTCACTCAGACTAC ATCAATGCCAACTACATTGATGGGTACCACCGACCCCGGCACTACATCGCAACTCAAGGTCCAATGCAAG AGACCGTGAAGGACTTTTGGAGAATGATCTGGCAGGAAAACTCTGCCAGCATCGTCATGGTCACAAACCT TGTGGAAGTGGGCAGGGTGAAGTGTGTCCGATACTGGCCAGATGACACAGAGGTCTATGGAGACATTAAA GTCACCCTAATAGAAACAGAGCCCCTGGCAGAATACGTCATCCGTACCTTCACAGTCCAGAAGAAAGGCT ACCATGAGATCCGGGAGCTCCGCCTCTTCCACTTCACCAGCTGGCCTGACCATGGTGTTCCCTGCTATGC CACTGGCCTTCTGGGCTTTGTCCGCCAGGTCAAGTTCCTCAACCCCCCAGAAGCCGGGCCCATAGTGGTC CACTGCAGTGCTGGAGCCGGGAGGACTGGCTGCTTCATTGCCATTGATACCATGCTCGACATGGCTGAGA ATGAAGGGGTTGTGGACATCTTCAACTGTGTGCGTGAACTCCGGGCACAGAGGGTCAACCTGGTGCAGAC AGAGGAACAGTACGTGTTTGTGCATGATGCCATCCTGGAAGCATGCCTCTGCGGCAACACGGCCATCCCC GTGTGCGAGTTCCGCTCTCTCTACTACAACATCAGCAGGCTGGACCCGCAGACCAACTCCAGCCAGATCA AAGATGAGTTTCAGACACTCAACATCGTGACACCTCGGGTGCGGCCTGAGGACTGCAGCATTGGGCTCTT GCCCCGGAACCATGACAAGAACCGGAGCATGGATGTCCTGCCTCTGGACCGCTGCCTACCCTTCCTCATC TCAGTAGATGGAGAATCCAGCAACTACATCAATGCAGCACTGATGGATAGCCACAAGCAGCCCGCCGCCT TTGTGGTCACCCAGCACCCTCTACCCAACACTGTGGCAGACTTCTGGAGGCTGGTGTTTGATTATAATTG TTCATCTGTGGTGATGCTGAATGAGATGGACACTGCTCAGCTCTGTATGCAGTACTGGCCCGAGAAGACC TCTGGGTGTTATGGTCCCATCCAGGTGGAGTTCGTCTCTGCAGACATTGATGAGGACATCATCCACAGAA TCTTCCGTATCTGTAACATGGCTCGGCCACAGGACGGTTACCGTATTGTCCAGCACCTCCAGTACATTGG CTGGCCTGCATACCGAGACACACCGCCCTCCAAGCGCTCTCTGCTCAAAGCGGTCCGACGGCTGGAGAAA TGGCAGGAGGAATATGATGGAAGAGAGGGTCGCACTGTGGTCCACTGCCTAAACGGGGGAGGCCGCAGCG GAACCTTCTGTGCTATCTGCAGCGTGTGCGAGATGATCCAACAGCAGAACATCATCGACGTGTTCCACAT CGTGAAGACCCTCCGCAACAACAAATCCAACATGGTGGAGACGCTGGAACAGTATAAATTTGTATATGAG GTGGCACTGGAATATTTAAGTTCTTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR210452 representing NM_001108603 Red=Cloning site Green=Tags(s) MLDPAVPTGSFMMVNSSGRASGQKAHLLLPTLKENDTHCIDFHYYFSSRDRSSPGALNVYVKVNGGPQGN PVWNVSGVVTEGWVKAELAISTFWPHFYQVIFESVSLKGHPGYIAVDEVRVLAHPCRKAPHFLRLQNVEV NVGQNATFQCIAGGKWSQHDKLWLQQWNGRDTALMVTRVVNHRRFSATVSVADTSQRSVSKYRCVIRSDG GSGVSNYAELIVKEPPTPIAPPELLAVGATYLWIKPNANSIIGDGPIILKEVEYRTTTGTWAETHIVDSP NYKLWHLDPDVEYEIRVLLTRPGEGGTGPPGPPLTTRTKCAVPGAVPLESIQGGPFEEKIYIQWKPPNET NGVITLYEINYKAVGSLDPSADLSSQRGKVFKLRNETHHLFVGLYPGTTYSFTIKASTAKGFGPPVTTRI ATKISAPSMPEYDADSPLNETDTTITVMLKPAQSRGAPVSVYQLVVKEERLQKSRRAADIIECFSVPVSY RNASNLDSLHYFAAELKPSNLPVTQPFTVGDNKTYNGYWNPPLSPLKSYSIYFQALSKANGETKINCVRL ATKGASTQNSNTVEPEKQVDNTVKMAGVIAGLLMFIIILLGVMLTIKRRKLAKKQKETQSGAQREMGPVA STDKPTTKLSTNRNDEGFSSSSQDVNGFTDGSRGELSQPTLTIQTHPYRTCDPVEMSYPRDQFQPAIRVA DLLQHITQMKRGQGYGFKEEYEALPEGQTASWDTAKEDENRNKNRYGNIISYDHSRVRLLVLDGDPHSDY INANYIDGYHRPRHYIATQGPMQETVKDFWRMIWQENSASIVMVTNLVEVGRVKCVRYWPDDTEVYGDIK VTLIETEPLAEYVIRTFTVQKKGYHEIRELRLFHFTSWPDHGVPCYATGLLGFVRQVKFLNPPEAGPIVV HCSAGAGRTGCFIAIDTMLDMAENEGVVDIFNCVRELRAQRVNLVQTEEQYVFVHDAILEACLCGNTAIP VCEFRSLYYNISRLDPQTNSSQIKDEFQTLNIVTPRVRPEDCSIGLLPRNHDKNRSMDVLPLDRCLPFLI SVDGESSNYINAALMDSHKQPAAFVVTQHPLPNTVADFWRLVFDYNCSSVVMLNEMDTAQLCMQYWPEKT SGCYGPIQVEFVSADIDEDIIHRIFRICNMARPQDGYRIVQHLQYIGWPAYRDTPPSKRSLLKAVRRLEK WQEEYDGREGRTVVHCLNGGGRSGTFCAICSVCEMIQQQNIIDVFHIVKTLRNNKSNMVETLEQYKFVYE VALEYLSSF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001108603
ORF Size	3807 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001108603.1, NP_001102073.1
RefSeq Size	5787 bp
RefSeq ORF	3810 bp
Locus ID	362263
MW	142.5 kDa

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