Ptprt (NM_001108603) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN210452 representing NM_001108603 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGACCCAGCTGTGCCCACAGGGTCCTTCATGATGGTGAATAGCTCTGGAAGAGCTTCAGGCCAGA AAGCCCATCTTCTTCTACCAACCTTGAAGGAGAATGATACCCACTGCATTGACTTCCATTACTACTTCTC CAGCCGAGATCGCTCCAGTCCGGGAGCCTTGAATGTCTACGTGAAGGTGAATGGTGGTCCCCAAGGGAAC CCTGTCTGGAACGTGTCTGGGGTCGTCACTGAGGGCTGGGTGAAGGCAGAACTTGCCATCAGTACCTTCT GGCCTCATTTCTACCAGGTGATATTTGAATCCGTCTCTTTGAAAGGTCATCCTGGTTACATCGCTGTGGA CGAAGTTCGAGTCCTTGCTCATCCATGCAGAAAAGCACCTCATTTCCTGCGACTCCAAAATGTTGAGGTG AATGTGGGGCAGAACGCCACGTTTCAGTGCATTGCCGGTGGGAAGTGGTCCCAGCATGACAAACTTTGGC TCCAGCAATGGAATGGCAGAGACACGGCCCTCATGGTCACCCGGGTGGTCAACCACAGGCGCTTCTCAGC CACAGTGAGTGTGGCAGACACCTCTCAACGCAGTGTCTCCAAGTATCGCTGTGTGATCCGCTCAGACGGT GGGTCTGGCGTATCCAACTATGCAGAGCTGATCGTGAAAGAGCCTCCCACACCTATTGCTCCCCCAGAAC TACTGGCAGTGGGGGCCACCTACCTGTGGATTAAACCAAACGCCAACTCTATTATTGGGGACGGCCCCAT CATTCTGAAGGAGGTAGAATACCGCACGACCACAGGCACTTGGGCTGAGACCCACATCGTGGACTCTCCC AACTACAAGCTCTGGCATCTGGACCCCGATGTGGAGTACGAGATCCGGGTGCTGCTCACACGACCAGGAG AGGGGGGCACAGGACCACCAGGACCTCCCCTCACTACCAGGACCAAGTGTGCCGTTCCAGGAGCTGTTCC TCTGGAGTCCATCCAAGGAGGACCCTTTGAGGAGAAGATCTATATCCAGTGGAAACCTCCCAATGAGACC AATGGGGTCATCACACTCTATGAGATTAATTACAAGGCTGTTGGCTCACTGGATCCAAGTGCTGACCTGT CCAGCCAGAGGGGGAAAGTGTTCAAACTCCGGAATGAAACCCATCACCTCTTTGTGGGTCTGTACCCTGG GACTACCTACTCTTTCACCATCAAGGCCAGCACAGCCAAGGGCTTTGGACCCCCAGTCACCACTCGGATT GCCACCAAGATTTCAGCTCCATCAATGCCCGAATATGATGCAGATAGCCCACTCAATGAAACAGATACGA CCATCACAGTGATGCTCAAACCTGCCCAGTCCCGGGGAGCCCCTGTCAGCGTTTACCAGTTGGTTGTCAA GGAGGAGCGACTTCAGAAGTCTCGGAGAGCAGCTGACATCATCGAGTGTTTCTCAGTGCCCGTGAGCTAC CGGAATGCCTCCAACCTCGATTCTCTACACTACTTTGCTGCTGAGCTGAAGCCCTCCAATCTGCCTGTCA CCCAGCCCTTCACAGTGGGAGACAACAAGACCTACAATGGCTACTGGAATCCACCCCTCTCCCCATTAAA GAGCTACAGCATCTACTTCCAGGCTCTCAGCAAAGCAAATGGAGAGACAAAAATCAACTGCGTGCGTCTG GCTACAAAAGGTGCTTCCACCCAGAATTCTAACACTGTGGAGCCCGAGAAGCAGGTCGACAACACAGTGA AGATGGCTGGTGTGATTGCTGGCCTCCTCATGTTCATCATAATTCTACTGGGGGTGATGCTGACCATCAA AAGGAGGAAGCTGGCTAAGAAGCAGAAGGAGACCCAGAGTGGAGCCCAGAGGGAGATGGGGCCTGTGGCC TCGACTGACAAACCTACCACCAAGCTCAGCACCAACCGCAACGATGAAGGCTTCTCCTCCAGCTCTCAGG ATGTTAACGGATTCACAGATGGCAGCCGTGGAGAGCTGTCTCAGCCCACCCTCACCATTCAGACTCACCC CTACCGGACTTGCGACCCTGTAGAGATGAGCTACCCCCGAGACCAGTTCCAGCCCGCCATCCGGGTAGCA GACCTGCTTCAACACATCACCCAGATGAAGAGAGGCCAGGGCTACGGGTTCAAGGAAGAATATGAGGCCT TACCAGAGGGACAGACAGCTTCGTGGGACACAGCCAAGGAAGATGAAAACCGCAATAAGAATCGATATGG GAACATCATATCTTATGACCACTCCCGAGTGAGGCTGTTGGTGCTGGATGGAGACCCTCACTCAGACTAC ATCAATGCCAACTACATTGATGGGTACCACCGACCCCGGCACTACATCGCAACTCAAGGTCCAATGCAAG AGACCGTGAAGGACTTTTGGAGAATGATCTGGCAGGAAAACTCTGCCAGCATCGTCATGGTCACAAACCT TGTGGAAGTGGGCAGGGTGAAGTGTGTCCGATACTGGCCAGATGACACAGAGGTCTATGGAGACATTAAA GTCACCCTAATAGAAACAGAGCCCCTGGCAGAATACGTCATCCGTACCTTCACAGTCCAGAAGAAAGGCT ACCATGAGATCCGGGAGCTCCGCCTCTTCCACTTCACCAGCTGGCCTGACCATGGTGTTCCCTGCTATGC CACTGGCCTTCTGGGCTTTGTCCGCCAGGTCAAGTTCCTCAACCCCCCAGAAGCCGGGCCCATAGTGGTC CACTGCAGTGCTGGAGCCGGGAGGACTGGCTGCTTCATTGCCATTGATACCATGCTCGACATGGCTGAGA ATGAAGGGGTTGTGGACATCTTCAACTGTGTGCGTGAACTCCGGGCACAGAGGGTCAACCTGGTGCAGAC AGAGGAACAGTACGTGTTTGTGCATGATGCCATCCTGGAAGCATGCCTCTGCGGCAACACGGCCATCCCC GTGTGCGAGTTCCGCTCTCTCTACTACAACATCAGCAGGCTGGACCCGCAGACCAACTCCAGCCAGATCA AAGATGAGTTTCAGACACTCAACATCGTGACACCTCGGGTGCGGCCTGAGGACTGCAGCATTGGGCTCTT GCCCCGGAACCATGACAAGAACCGGAGCATGGATGTCCTGCCTCTGGACCGCTGCCTACCCTTCCTCATC TCAGTAGATGGAGAATCCAGCAACTACATCAATGCAGCACTGATGGATAGCCACAAGCAGCCCGCCGCCT TTGTGGTCACCCAGCACCCTCTACCCAACACTGTGGCAGACTTCTGGAGGCTGGTGTTTGATTATAATTG TTCATCTGTGGTGATGCTGAATGAGATGGACACTGCTCAGCTCTGTATGCAGTACTGGCCCGAGAAGACC TCTGGGTGTTATGGTCCCATCCAGGTGGAGTTCGTCTCTGCAGACATTGATGAGGACATCATCCACAGAA TCTTCCGTATCTGTAACATGGCTCGGCCACAGGACGGTTACCGTATTGTCCAGCACCTCCAGTACATTGG CTGGCCTGCATACCGAGACACACCGCCCTCCAAGCGCTCTCTGCTCAAAGCGGTCCGACGGCTGGAGAAA TGGCAGGAGGAATATGATGGAAGAGAGGGTCGCACTGTGGTCCACTGCCTAAACGGGGGAGGCCGCAGCG GAACCTTCTGTGCTATCTGCAGCGTGTGCGAGATGATCCAACAGCAGAACATCATCGACGTGTTCCACAT CGTGAAGACCCTCCGCAACAACAAATCCAACATGGTGGAGACGCTGGAACAGTATAAATTTGTATATGAG GTGGCACTGGAATATTTAAGTTCTTTTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001108603
Insert Size	3810 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001108603.1, NP_001102073.1
RefSeq Size	5787 bp
RefSeq ORF	3810 bp
Locus ID	362263

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