United States
Germany
Japan
United Kingdom
China
Dok1 (NM_001025416) Rat Tagged ORF Clone
CAT＃: RR210723
- TrueORF®
Dok1 (Myc-DDK-tagged ORF) - Rat docking protein 1 (Dok1), (10 ug)
"NM_001025416" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 6,180.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Dok-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR210723 representing NM_001025416
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGGGGCTTTGATGGAGGGTCCGCTTTTTCTGCAGAGTCAGCGCTTCGGGACCAAGAGGTGGAAGA AAACTTGGGCTGTGCTTTACCCAGCCAGCCCTCACGGCGTGGCGCGGCTGGAATTCTTTGATCACAAGGG GTCGAGCTCTGGAGGGGGTCGAGGCGGCTCTCGCCGTCTAGACTGCAAGATGATACGCCTGGCTGAATGT GTGAGCGTGGTGCCTGTGACTGTGGAGAGTCCCCCTGAGCCCGGCGCCTCTGCCTTCCGCCTGGACACCG CACAACGCTCGCACCTGCTTGCAGCGGACGCCGCATCCAGCACTGCCTGGGTGCAGATTTTATGCAGAAC CGCTTTTCCGAAAGGCGGCTGGGCTTTGGCTCAGACGGAGAACCCACCTAAGTTTTCTGCCCTGGAGATG CTGGAGAATTCGCTGTACAGCCCCACCTGGGAAGGATCCCAATTCTGGGTAACCTCGCAGAAGACCGAGG CTTCTGAACGCTGCGGCTTGCAAGGCTCCTACGTACTGAGGGTAGAAGCTGAGAAGCTGACCCTTCTGAC TTTGGGTGCACAGAGTCAAATCCTGGAGCCGCTCCTTTTCTGGCCCTACACTCTGTTGCGACGCTATGGC CGAGACAAGGTAATGTTCTCCTTTGAAGCGGGTCGCCGCTGCCCCTCTGGCCCTGGGACCTTCACCTTCC AGACTGCCCAGGGAAATGACATCTTTCAGGCAGTTGAGGCTGCTATCCAGCAGCAGAAAGCCCAAGGAAA GGTGGGACAGGGACAAGATATCACCAGAACTGACTCCCATGATGGGGAGACAGAGGGGAAGATGGCTCCC ACTCCTGTTCCCCAGGAACCCCTGGGCAGCCCTCCAGCCCTATATGCAGAGCCTTTAGACTCCCTGCGAA TTCCTCCAGGCCCTTCTCAGGACTCTTTGTATTCAGACCCCCTGGGCAGCACCCCTGCTGGGGCAGGGGA AGGGGTGCAGCGGAAGAAACCTCTCTATTGGGATTTGTATGGGCACGTGCAGCAGCAGTTGCTGAAAACC AAGCTGATAGACTCCAAAGAGGACCCCATCTATGATGAGCCTGAGGGCCTGGCCCCTGCCCCTCTCCGGG GCCTTTATGATCTGCCTCAGGAGCCTAAGGATGCATGGTGGTGCCAGGCTCGGCTGAAGGAAGAGGGCTA TGAGCTCCCTTACAACCCCGCCACCGACGACTACGCTGTGCCACCCCCTCGGAGCTCAAAGCCTACCCCT GCCCCCAAGCCACAGGGCTTGATCCTTCCTGAATCTGGTACCACAGCTGGCAGTGGCAGCAAAGGCTCAG ATACAGCTCTGTACAGCCAGGTCCAGAAAAGTGGGACCCCAGGGAGATGGGACTGTGGACTCTCTAGAGT AGGGAATGACAGGGTGGGGGTCAAGTCAGAGGGTTCCACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR210723 representing NM_001025416
Red=Cloning site Green=Tags(s) MDGALMEGPLFLQSQRFGTKRWKKTWAVLYPASPHGVARLEFFDHKGSSSGGGRGGSRRLDCKMIRLAEC VSVVPVTVESPPEPGASAFRLDTAQRSHLLAADAASSTAWVQILCRTAFPKGGWALAQTENPPKFSALEM LENSLYSPTWEGSQFWVTSQKTEASERCGLQGSYVLRVEAEKLTLLTLGAQSQILEPLLFWPYTLLRRYG RDKVMFSFEAGRRCPSGPGTFTFQTAQGNDIFQAVEAAIQQQKAQGKVGQGQDITRTDSHDGETEGKMAP TPVPQEPLGSPPALYAEPLDSLRIPPGPSQDSLYSDPLGSTPAGAGEGVQRKKPLYWDLYGHVQQQLLKT KLIDSKEDPIYDEPEGLAPAPLRGLYDLPQEPKDAWWCQARLKEEGYELPYNPATDDYAVPPPRSSKPTP APKPQGLILPESGTTAGSGSKGSDTALYSQVQKSGTPGRWDCGLSRVGNDRVGVKSEGST TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001025416 |
| ORF Size | 1440 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001025416.1, NP_001020587.1 |
| RefSeq Size | 1757 bp |
| RefSeq ORF | 1443 bp |
| Locus ID | 312477 |
| UniProt ID | Q4QQV2 |
| MW | 52.2 kDa |
| Gene Summary | DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN210723 | Dok1 (untagged ORF) - Rat docking protein 1 (Dok1), (10 ug) |
CNY 6,180.00 |
|
| RR210723L3 | Lenti ORF clone of Dok1 (Myc-DDK-tagged ORF) - Rat docking protein 1 (Dok1), (10 ug) |
CNY 8,080.00 |
|
| RR210723L4 | Lenti ORF clone of Dok1 (mGFP-tagged ORF) - Rat docking protein 1 (Dok1), (10 ug) |
CNY 8,840.00 |
