Ros1 (NM_012874) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ROS1C
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR210745 representing NM_012874 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAGGATCCGCTGGCTCACCCCAAAACCTGCGACCTTTGTGGTCCTTGGGTGCGTATGGATTTCCG TGGCGCAGGGTACCATTCTGAGCAGCTGCCTAACGTCCTGTGTAACTAACTTGGGCAGGCAGCTTGACAG CGGCACCCGGTACAATCTGAGTGAGGCATGCATCCAAGGATGTCAGTTTTGGAACTCTATAGATCAGGAG AAGTGTGCTTTGAAGTGTAATGATACATATGTCACCATTTGTGAGAGGGAGTCCTGTGAGGTCGGCTGCA GCAACGCGGAGGGTAGCTACGAAGAGGAAGTGCTGGACAACACAGAGCTTCCTACAGCACCCTTCGCATC TTCCATTGGAAGTAACGGGGTGACATTACGATGGAACCCTGCCAACATCTCTGGAGTAAAATACATCATT CAGTGGAAATATGCCCAACTTCCGGGAAGCTGGGCTTACACAGAAACTGTGTCTAAGCTCTCATACATGG TGGAACCCCTGCATCCATTTACTGAATATATTTTTCGAGTGGTTTGGATTTTCACAGCCCAGCTGCACCT TTATTCCCCGCCAAGTCCCAGTTACAGGACTCATCCTTATGGAGTTCCAGAAACTGCGCCTTTCATCACG AACATCGAAAGCTCGAGCCCTGACACTGTGGAGGTCAGCTGGGCTCCACCCTATTTCCCAGGTGGACCTA TTTTGGGTTATAATTTAAGGCTGATCAGTAAAACTCAAAAATTAGATTCAGGGACACAGAGAACCAGTTT CCAGTTTTATTCTACTCTTCCAAACACCACTTACAGGTTTTCTATCGCAGCAGTCAATGAAGTCGGTGAG GGGCCAGAAGCAGAATCTATGATTACCACTCCATCCCCAGCAGTTCAAGAAGAAGAACAATGGCTCTTTT TATCCAGAAAAACTTCTCTAAGAAAGAGGTCTTTGAAGTACTTAGTAGACGAAGCACATTGCCTTTGGTC AGATGCTATACGTCATAATATTACAGGAATATCAGTCAACACTCAGCAGGAAGTGGTTTATTTCTCAGAA GGAACCATCATATGGATGAAGGGGGCTGCTAACATGTCTGATGTGTCTGACCTGAGGATCTTTTATCGAG GCTCAGCTCTAGTCTCTTCTATCTCTGTAGACTGGCTTTACCAAAGGATGTATTTCATCATGGATAATCG GGTGCATGTCTGTGACTTAAAGCATTGCTCAAATCTTGAGGAAATCACTCCATTCTCTATTGTTGCACCT CAAAAAGTTGTGGTTGATTCCTACAATGGGTATGTCTTTTATCTCCTAAGAGACGGCATTTATAGAGTCC ATCTTCCTTTGCCGTCTGTCAGGGACACCAAAGCTGTGCGTATTGTGGAGAGTGGCACATTAAAGGACTT CGCAGTAAAGCCGCAGTCCAAGCGAATCATTTACTTCAATGGCACCATGCAAGTCTTCATGTCGACATTT CTGGATGGCTCGGCATTCCACAGGGTTCTGCCGTGGGTCCCCCTTGCGGATGTGAAGAGCTTTGCTTGTG AAAACAATGACTTCCTCATCACAGATGGCAAGGCCATTTTCCAACAGGACTCTCTGTCTTTCAATGAGTT CATCGTGGGATGTGACCTGAGTCACATAGAAGAATTTGGGTTTGGTAACTTGGTCATCTTTGGCTCCTCC GTCCAGTCGTACCCTCTGCCAGGCCATCCACAGGAGGTCTCGGTGCTGTTTGGTTCTCGAGAGGCCCTTA TTCAGTGGAAGCCTCCGATTCTCGCCATAGGAGCCAGTCCTTCCGCCTGGCAGAACTGGACTTATGAGGT CAAAGTTTCCTCCCAGGACATTCTGGAAACCACTCAAGTTTTCTTGAACATAAGCAGGACTGTGCTGAAT GTACCCAAGCTGCAAAGTTCTACAAAGTACATGGTGTCTGTGCGAGCAAGTTCTCCTAAAGGCCCAGGCC CATGGTCAGAACCCTCAGTGGGTACTACCTTGGTACCAGCCACTGAGCCACCGTTCATCATGGCTGTGAA AGAAGATGGGCTTTGGAGCAAACCACTCAGTAGTTTTGGCCCAGGAGAGTTCCTATCCTCTGACGTAGGA AACGTGTCAGATATGGATTGGTATAACAACAGCCTCTACTACAGTGACACAAAAGGCAATGTGTATGTGC GGCCTCTGAATGGGATGGATATCTCGGAGAATTACCACATATCCAGCATTGCAGGAGCTTGTGCCTTGGC CTTTGAATGGCTGGGTCACTTTCTCTACTGGGCTGGGAAGACATATGTGATTCAAAGGCAGTCTGTGTTA ACGGGACACACAGACATTGTGACTCACGTGAAGCTGTTGGTGAATGACATGGCCGTGGATCCAGTTGGTG GCTATCTGTACTGGACGACGCTCTACTCGGTTGAAAGCACCAGACTCAATGGAGAAAGTTCTCTTGTACT ACAGGCTCAGCCCTGGCTCTCTGGAAAAAAGGTTATTGCTCTAACATTAGACCTCAGCGATGGGCTCCTG TACTGGCTGGTGCAGGACAATCAGTGTATTCACCTGTACACGGCTGTTCTCCGGGGATGGAGTGGTGCGG ATGCTACCATCACCGAGTTTGCAGCCTGGAGTACTTCTGAAATTTCCCAGAATGCACTGATGTACTACAG CGGTAGACTCTTCTGGATCAATGGCTTTAGGATCATCACAGCACAGGAAATAGGTCAGAGAACCAGCGTG TCTGTTTCTGAGCCAGGGAAATTCAATCAGTTTACGATCATACAGACATCCCTCAAGCCTCTGCCAGGGA ACTTTTCCTCTACTCCCACGGTTATCCCAGATTCTGTTCAGGAGTCCTCATTTCGAATTGAAGGACACAC TTCAAGTTTCCGAATCCTGTGGAATGAGCCCCCTGCGGTGGACTGGGGCATAGTTTTCTACAGTGTGGAA TTTAGTGCTCATTCTAAGTTCCTGGCTATTGAACAACAGTCTTTACCTGTTTTTACTGTGGAAGGACTGG AGCCCTATGCCTTATTTAATCTTTCTGTCACTCCTTATACCTATTGGGGAAAAGGTCAAAAAACATCTCT ATCATTTCGAGCGCCTGAATCAGTTCCGTCAGCACCAGAGAACCCCAGAATATTTATATTGTCACTTGGA AGATACACCAGGAAGAATGAAGTCGTGGTAGAGTTTAGGTGGAATAAACCTAAGCATGAAAATGGAGTGC TAACCAAATCTGAAATCTTCTACCACATATCTAAACAAAGTGGCACAAATAAATCAACGGAAGACTGGGT ATCTGTCAGCGTTACACCGCCGGTGATGTCTTTTCAACTTGAAGCCATGAGTCCTGGGTATATTGTTTCC TTCCAGGTTCGAGTCTTCACCTCCAAAGGGCCAGGACCATTTTCTGATATAGTGATGTCTAAAACATCAG AAATCAAGCCATGTCCATATCTCATATCTCTTCTTGGCAATAAGATTGAGTTCTTAGACATGGACCAAAA TCAAGTTGTGTGGACATTTTCCCTGGAGGGAGCCGTCAGCACAGTGGGGTACACAGCGGATGATGAAATG GGGTATTTCGCTCAAGGAGATGCACTCTTCCTTCTGAATTTGCACAATCATTCCAGCTCCAAGCTTTTCC AGGACGTGCTGGCTTCTGACATTGCGGTTATTGCTGTTGACTGGATCGCAAGGCACCTCTACTTTGCTCT GAAAGCATCGCAAGATGGAACACAGATATTCGATGTTGACCTTGAACACAAGGTGAAATCCCCCAGGGAG GTGAAGATTTGCAAAAGCCATACAGCAATAATTTCTTTCTCTATGTATCCCCTCTTAAGTCGCCTGTATT GGACAGAAGTTTCAGATCTGGGCTACCAGATGTTCTACTGCAATATTAGCAGTCACACCTTGCATCACGT TCTACAACCCAAGGCCTCAAACCAGCATGGAAGGAGACAGTGTTCTTGTAATGTGACAGAATCCGAGTTA AGTGGGGCAATGACTGTGGACACGTCTGATCCAGACAGACCTTGGATATACTTTACCAAACAGCAAGAGA TCTGGGCCATGGATCTGGAAGGATGTCAGTGTTGGAAAGTCATCATGGTACCTGCTACCCCTGGAAAAAG AATCATTAGTTTAACAGTGGATGGGGAGTTTATATATTGGATCACAACAATGAAGGACGACACAGAAATT TATCAAGCAAAGAAGGGAAGTGGGGCCATCCTCTCCCAGGTGAAGGCCCCCAGGAGTAAGCATATCTTGG CTTACAGTTCAGCTCTGCAACCTTTTCCAGATAAAGCATATCTGTCTGTAGCTTCCAATATGGTAGAAGC AAGTATATTGAATGCCACCAACACCAGCCTCATTCTCAAGTTACCTCCAGTCAAGACAAACCTCACGTGG CATGGAATTACCACTCCCACGTCAACATACCTGGTTTACTATATGGAGGCTAATAGGGCAAACAGCTCTG ACAGGAAACACAACATGTTGGAATCACAGGAGAATGTAGCCCGGATTGAAGGTCTGCAGCCATTTTCAAC ATACGTGATTCAGATAGCTGTGAAGAACTATTATTCTGATCCTTTAGAACATCTCTCTCTGGGAAAAGAG ATTCAAGGAAAAACTAAAAGTGGAGTGCCCGGGGCAGTTTGTCATATCAATGCAACTGTGCTGTCGGACA CCAGTCTTCTTGTATTCTGGACAGAATCGCATAAACCAAACGGACCCAAAGAGTTAGTCCGCTATCAGTT GGTTATGTCATACCTGGCTCCGATTCCTGAGACTCCTCTAAGACAGGACGAATTTCCAAGCGCCAGGCTT TCTCTACTTGTCACTAAACTCTCTGGTGGACAACAATATGTGCTGAAGATCCTTGCCTGCCACTCAGAGG AAATGTGGTGTACTGAGAGTCATCCTGTCAGTGTCAACATGTTTGACACACCGGAGAAACCTTCTGCCTT GGTTCCAGAGAACACTAGTCTGCTGTTGGATTGGAAGGCTCCGTCTAACGCTAACCTCACCAGATTTTGG TTTGAACTCCAGAAGTGGAAGTATAGTGAGTTTTACCATGTCAAGGCTTCATGCAGCCAAGGTCCAGTTT ATGTCTGTAACATCGCAAATCTGCAGCCTTACACTCCTTATAACATCCGAGTGGTGGTGGTCTATACGAC AGGAGAAAATAGCTCCTCGATTCCCGAGAGCTTCAAGACAAAAGCTGGAGTCCCAAGCAAACCAGGGATT CCTAAGTTACTAGAAGGGAGTAAAAATTCAATCCAGTGGGAAAAAGCCGAAGATAACGGGAACAGATTGA TGTACTACACCCTGGAGGTCAGAAAAAGCATTTCAAATGACTCACGGGACCAGAGTTTAAGGTGGACGGC GGTGTTTAATGGGTCCTGCAGTAGCATTTGCACATGGAGGTCAAAAAACCTAAAAGGAACTTTCCAGTTC AGAGCAGTAGCGTCAAATGCTATTGGATTTGGAGAATACAGTGAAATCAGTGAAGATATTACATTAGTGG AAGATGGTTTTTGGATAACAGAAACAAGTTTTATACTTACTATCATAGTTGGGATATTTCTGGTTGCCAC AGTCCCACTGACCTTTGTCTGGCATAGAAGCTTGAAAAACCACAAAGCTACCAAGGAAGGCCTCTCAGTT CTCAACGACAATGACCAAGAGTTGGCTGAGCTTCGAGGACTGGCGGCTGGAGTGGGCCTGGCCAATGCCT GCTATGCAGTACATACTCTTCCAACCCAAGAGGAGATTGAAAGTCTTCCCGCCTTCCCTCGGGAGAAGCT GAGCCTGCGCCTTCTGTTGGGAAGTGGAGCTTTTGGAGAAGTGTACGAGGGCACAGCTGTAGACATCCTA GGACGGGGAAGTGGAGAAATCAAGGTGGCCGTGAAGACCCTGAAGAAAGGTTCGACAGACCAGGAGAAGA TCGAGTTCCTGAAGGAGGCACACCTGATGAGCAAGTTTAATCACCCCAACATTCTGAAGCAGCTGGGAGT CTGTCTGCTGAGTGAACCCCAGTACATTATCCTGGAACTGATGGAAGGGGGAGACCTTCTAAGCTATCTG CGCAAAGCCCGAGGGACAACGTTGTCTGGCCCTTTACTCACATTGGCTGACCTGGTAGAGCTGTGTGTAG ATATTTCAAAAGGCTGCGTCTACTTGGAGCAGATGCACTTCATTCACAGGGATCTGGCAGCTCGGAATTG CCTTGTGTCTGTGAAAGACTATACCAGTCCTCGGGTAGTCAAGATCGGTGACTTTGGTTTGGCAAGGGAA ATCTATAAGCATGATTATTATAGAAAGAGAGGGGAAGGCCTGCTTCCTGTCCGGTGGATGGCTCCTGAAA ACTTGATGGATGGAATCTTCACTTCCCAGTCTGATGTATGGTCTTTTGGAATTTTGGTTTGGGAGATTTT AACTCTTGGTCATCAACCTTATCCAGCGCATTCCAACCTTGATGTTTTAAACTATGTGCAAGCAGGAGGG AGACTGGAGCCACCGAGAAACTGTCCTGATGATCTGTGGAATTTAATGTTCCGATGTTGGGCCCAAGAAC CTGACCAAAGACCCACTTTCTATAACATTCAAGACCAGCTTCAGTTATTCAGAAATGTTTCCTTAAACAA TGTTTCTCACTGTGGACAAGCAGCTCCTGCTGGTGGAGTCATCAACAAAGGCTTTGAAGGTGAAGACAAT GAAATGGCCACTTTGAATTCAGATGACACGATGCCAGTTGCCTTGATGGAAACCAGGAACCAAGAAGGAT TAAATTATATGGTACTTGCCACAAAGTGTAGCCAAAGTGAGGATCGTTATGAGGGTCCTCTAGGCTCTAA GGAATCTGGGTTGCATGATCTGAAGAAAGACGAGAGGCAACCAGCAGACAAAGATTTCTGCCAGCAACCA CAGGTGGCTTATGGCTCTCCTGGCCACTCTGAAGGCCTGAACTATGCCTGTCTTGCTCACAGTGGACATG GAGATGTGTCTGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR210745 representing NM_012874 Red=Cloning site Green=Tags(s) MKRIRWLTPKPATFVVLGCVWISVAQGTILSSCLTSCVTNLGRQLDSGTRYNLSEACIQGCQFWNSIDQE KCALKCNDTYVTICERESCEVGCSNAEGSYEEEVLDNTELPTAPFASSIGSNGVTLRWNPANISGVKYII QWKYAQLPGSWAYTETVSKLSYMVEPLHPFTEYIFRVVWIFTAQLHLYSPPSPSYRTHPYGVPETAPFIT NIESSSPDTVEVSWAPPYFPGGPILGYNLRLISKTQKLDSGTQRTSFQFYSTLPNTTYRFSIAAVNEVGE GPEAESMITTPSPAVQEEEQWLFLSRKTSLRKRSLKYLVDEAHCLWSDAIRHNITGISVNTQQEVVYFSE GTIIWMKGAANMSDVSDLRIFYRGSALVSSISVDWLYQRMYFIMDNRVHVCDLKHCSNLEEITPFSIVAP QKVVVDSYNGYVFYLLRDGIYRVHLPLPSVRDTKAVRIVESGTLKDFAVKPQSKRIIYFNGTMQVFMSTF LDGSAFHRVLPWVPLADVKSFACENNDFLITDGKAIFQQDSLSFNEFIVGCDLSHIEEFGFGNLVIFGSS VQSYPLPGHPQEVSVLFGSREALIQWKPPILAIGASPSAWQNWTYEVKVSSQDILETTQVFLNISRTVLN VPKLQSSTKYMVSVRASSPKGPGPWSEPSVGTTLVPATEPPFIMAVKEDGLWSKPLSSFGPGEFLSSDVG NVSDMDWYNNSLYYSDTKGNVYVRPLNGMDISENYHISSIAGACALAFEWLGHFLYWAGKTYVIQRQSVL TGHTDIVTHVKLLVNDMAVDPVGGYLYWTTLYSVESTRLNGESSLVLQAQPWLSGKKVIALTLDLSDGLL YWLVQDNQCIHLYTAVLRGWSGADATITEFAAWSTSEISQNALMYYSGRLFWINGFRIITAQEIGQRTSV SVSEPGKFNQFTIIQTSLKPLPGNFSSTPTVIPDSVQESSFRIEGHTSSFRILWNEPPAVDWGIVFYSVE FSAHSKFLAIEQQSLPVFTVEGLEPYALFNLSVTPYTYWGKGQKTSLSFRAPESVPSAPENPRIFILSLG RYTRKNEVVVEFRWNKPKHENGVLTKSEIFYHISKQSGTNKSTEDWVSVSVTPPVMSFQLEAMSPGYIVS FQVRVFTSKGPGPFSDIVMSKTSEIKPCPYLISLLGNKIEFLDMDQNQVVWTFSLEGAVSTVGYTADDEM GYFAQGDALFLLNLHNHSSSKLFQDVLASDIAVIAVDWIARHLYFALKASQDGTQIFDVDLEHKVKSPRE VKICKSHTAIISFSMYPLLSRLYWTEVSDLGYQMFYCNISSHTLHHVLQPKASNQHGRRQCSCNVTESEL SGAMTVDTSDPDRPWIYFTKQQEIWAMDLEGCQCWKVIMVPATPGKRIISLTVDGEFIYWITTMKDDTEI YQAKKGSGAILSQVKAPRSKHILAYSSALQPFPDKAYLSVASNMVEASILNATNTSLILKLPPVKTNLTW HGITTPTSTYLVYYMEANRANSSDRKHNMLESQENVARIEGLQPFSTYVIQIAVKNYYSDPLEHLSLGKE IQGKTKSGVPGAVCHINATVLSDTSLLVFWTESHKPNGPKELVRYQLVMSYLAPIPETPLRQDEFPSARL SLLVTKLSGGQQYVLKILACHSEEMWCTESHPVSVNMFDTPEKPSALVPENTSLLLDWKAPSNANLTRFW FELQKWKYSEFYHVKASCSQGPVYVCNIANLQPYTPYNIRVVVVYTTGENSSSIPESFKTKAGVPSKPGI PKLLEGSKNSIQWEKAEDNGNRLMYYTLEVRKSISNDSRDQSLRWTAVFNGSCSSICTWRSKNLKGTFQF RAVASNAIGFGEYSEISEDITLVEDGFWITETSFILTIIVGIFLVATVPLTFVWHRSLKNHKATKEGLSV LNDNDQELAELRGLAAGVGLANACYAVHTLPTQEEIESLPAFPREKLSLRLLLGSGAFGEVYEGTAVDIL GRGSGEIKVAVKTLKKGSTDQEKIEFLKEAHLMSKFNHPNILKQLGVCLLSEPQYIILELMEGGDLLSYL RKARGTTLSGPLLTLADLVELCVDISKGCVYLEQMHFIHRDLAARNCLVSVKDYTSPRVVKIGDFGLARE IYKHDYYRKRGEGLLPVRWMAPENLMDGIFTSQSDVWSFGILVWEILTLGHQPYPAHSNLDVLNYVQAGG RLEPPRNCPDDLWNLMFRCWAQEPDQRPTFYNIQDQLQLFRNVSLNNVSHCGQAAPAGGVINKGFEGEDN EMATLNSDDTMPVALMETRNQEGLNYMVLATKCSQSEDRYEGPLGSKESGLHDLKKDERQPADKDFCQQP QVAYGSPGHSEGLNYACLAHSGHGDVSE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_012874
ORF Size	7014 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_012874.1, NP_037006.1
RefSeq Size	7902 bp
RefSeq ORF	7017 bp
Locus ID	25346
UniProt ID	Q63132
MW	261.7 kDa
Gene Summary	a proto-oncogene and a putative receptor-type tyrosine kinase that may be active in the lung, kidney and heart [RGD, Feb 2006]

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