Stag3 (NM_053730) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR211064 representing NM_053730 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTACTCTGTGGTCACCCTCCACCCAGCACCATGGCTCTTCCTCAGGCAGTATGTCCTCCCCTCTTC GAAAGTCTGTGAGATGTGCACAGATGGCCTTGTCTCCTTGTTCTTCCAACATCCAACCCTGTGATGACAG AGACTCCCAGGGAACTGCAGAATGGGATAGTTCCTCAACTAGTGAAGACAGTGACTTTGAAGATAGCTTA AGAAGAAATGTGAGGAAGAGAGCAGCAAAACGACCACCCAAAGCTATCCCAGTGGCAAAACATCCGAAGA AGCAGTCCCACATAGTACCTGGTGGGAATGACAAGAACAAGTCAGTGCCGCCAACCAGTGACCTTTTTGA TGCTGTGAAAGCTGCTAGAAGTTGTGCGCAGTCTTTGGTAGATGAGTGGCTAGAAAACTACAAGCAAGAT GAAAATGCAGGATTCTTGGAACTTGTTAATTTTTTCATCCGAGCCTGTGGATGTAAAAGCACTGTCACAC CCGAGATGTTCAAGACAATGTCCAACTCAGAGATCATCCAGCACCTAACAGAAGAGTTTAATGAGGACTC AGGTGACTATCCCCTGACAGCTCCAGGTCCATCCTGGAAGAAGTTCCAGGGAAGCTTCTGTGAGTTTGTG AAGACACTAGTCTGTCAGTGCCAGTACAGCCTCCTCTTTGACGGCTTTCCAATGGATGACCTTATCTCCC TGCTCATTGGCCTCTCAGATTCCCAGGTCCGAGCCTTTCGTCATACTAGTACTTTGGCTGCCATGAAGCT AATGACTTCTCTGGTAAAAGTTGCACTCCAGTTGAGTCTGCACAAAGACAACAATCAACGTCAGTATGAG GCAGAACGAAACAAGGGGCCAGAGCAGAGGGCACCAGAGCGGCTCGAGAGTCTGCTGGAGAAACGAAAAG AGTTCCAAGAGAATCAAGAGGAGATAGAGGGGATGATGAATGCCATCTTCAGGGGTGTCTTTGTTCATCG GTACAGGGACATCCTTCCTGAGATCCGTGCTGTCTGCATCGAGGAGATCGGGTGTTGGATGCAAAGCTAC AGCACCTCCTTTCTTAATGACAGCTACCTAAAATATATTGGCTGGACCCTGCATGACAAGCACAAGGAAG TCCGCCTAAAGTGTGTGAAGGCTCTGGCAGGGCTGTACAGCAACCAGGAGCTGAGTTCACGGATGGAGCT CTTTACTAATCGCTTCAAGGACCGGATGGTTTCCATGGTCATGGACAGAGAGAGTGAAGTAGCAGTGGAG GCCATCAGATTGCTGACCCTTATTCTGAAGAACATGGAGGGAGTACTGACTAGTGCAGATTGTGAGAAAA TTTACTCCATTGTATACATTTCTAATCGTGCCATGGCCTCTTCTGCAGGGGAATTTGTGTACTGGAAGAT TTTCCATCCTGAATGTGGGGCAAAAGCAGTGAGTGGCAGGGAGCGACGCCGGAGTCCACAAGCCCAGAGG ACTTTCATTTACCTTTTATTGGCCTTCTTTATGGAGAGTGAGCATCACGACCATGCTGCTTACCTAGTTG ACAGCTTGTGGGACTGTGCAGGGTCTTACCTGAAGGACTGGGAGAGTCTGACAAGTCTGTTGCTGCAGAA AGACCAGAATCTGGGTGATATGCAAGAGAGAATGTTGATAGAAATCCTGGTGTCCAGTGCCCGGCAAGCT GCAGAGGGTCACCCACCAGTGGGGCGCATCACTGGAAAGAAGAGTCTGACCGCCAAAGAACGCAAGCTTC AAGCTTATGATAAGGTGAAGCTGGCTGAGCACCTCATCCCCCTCTTGCCCCAGCTCCTTGCCAAGTTCTC AGCAGATGCAGAGAACGTTGCTCCCTTGCTCCGGCTGCTCAGTTACTTTGACCTCAACATTTATTGCACT CAGCGCTTGGAGAAGCACTTGGAGCTGCTTCTGCAACAACTCCAGGAGGTGGTGGTGAAGCACGTAGAGC CTGAGGTGCTTGAGGCTGCAGCACATGCCCTCTATTTGCTCTGCAAGCCAGAGTTCACCTTCTTCAGCAG AGTGGACTTCGCCAGAAGCCAATTAGTAGATCTGCTGACTGATAGATTCCAGCAGGAGCTTGACGACCTA ATGCAGTCATCCTTCCTAGATGAGGATGAGGTATACAGTCTGACAGCCACTCTGAAGCGTCTCTCTGCCT TTTACAATGCTCATGACCTGACTCGCTGGGAGATCTCTGAACCATGTTCTCGACTCCTCCGGAAGGCTGT AGACACAGGAGAAGTTCCTCACCAGGTGATTTTGCCAGCCTTGACTCTGGTATATTTTTCCATTCTCTGG ACAGTGACCCACATTTCAGAGTCTACTTCCCAAAAGCAGCTGATGAGTCTGAAGAAAAGAATGGTAGCCT TCTGTGAACTTTGCCAAAGCTGCCTCTCAGACGTGGACCCAGAGATCCAGGAGCAGGCTTTTGTTTTATT AAGTGACCTGCTTCTCATCTTCAGCCCCCAGATGGTTGTAGGGGGCCGGGATTTCCTTAGGCCTCTTGTC TTTTTTCCGGAAGCTACTCTCCAGTCGGAACTAGCCAGCTTCCTCATGGACCATGTCTTTCTCCAGCCTG GAGAATTGGGCAACGGTCAATCACAGGAGGATCACGTCCAAATAGAGCTTCTGCACCAGAGGCGCCGCCT GCTTGCAGGATTTTGTAAGCTGTTGCTTTATGGGGTATTGGAACTGGATGCGGCCTCAGATGTTTTCAAA CACTACAACAAGTTCTATGAAGATTATGGTGACATTATCAAGGAAACATTAACTCGGGCGAGACAAATTG ACCGATGTCAGTGCTCTCGGATCCTGCTCCTGAGCCTAAAGCAGCTCTACACAGAACTGATACAGGAGCA GGGGCCCCAGGACCTGACAGAACTGCCAGCCTTCATTGAGATGAGAGACCTGGCCCGGAGGTTTGCCTTG AGCTTTGGACCCCAGCAGCTCCATAACAGAGATCTTGTGGTCATGCTGCACAAGGAAGGCATCAAGTTCT CATTGTCTGAGCTTCCTCCTGCTGGCTCTTCTCGAGAGCCCCCAAATATTGCATTCCTGGAGCTTCTTTC AGAGTTCTCCCCCCGCCTCTTCCATCAGGACAAACAGCTACTACTATCATACCTGGAAAAGTGTCTGCAG CGTGTCTCCATGGCACCTAGCCATCCCTGGGGTCCAGTCACCACCTACTGCCACTCCCTCCATCTAGTAG AGAACACAGCAGAGGCCAGCTCTCAGGGGCCCCCCCACTCCAAGAAGAGGTGTATTGAAGTTCCCCGCAG GCTTCAGGAAGAAGAGTCTTCATCCCAGGGAGAAAGCCTTCAGCTGAACAGTGGCCCCACAACACCTACA CTCACCTCCACAGCAGTGAAGAGAAGGCAGTCTCCGAGGACAGTAGGCAAGAGGCAAAAAGGTGGACCAG GACCAGGACCAGGACCAGGACCAGGACCAGGACCAGGACCAGGACCAGGACCAGGACCAGGACCAGGACC AGAGCTGATCTGCAGTCAGCAGCTCTCAGGCACCCAGAGGTTGAAAATGTCGAGTGCACCGTGTTTCCAG ATTCGATGTGATCCTTCTGGCTCTGGCTTGGGCAAGCAGATGACCCGACTCAGCCTTATGGAAGAAGATG AGGAAGAAGAGCTGAGACTTCTGGATGAAGAATGGCAATGTGGAGACAAGCTACTTCATAGCCCTTCTTC TCCCAGTGAGCATGGGCTGGACCTATTAGATACAACAGAGCTGAACATGGAGGATTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR211064 representing NM_053730 Red=Cloning site Green=Tags(s) MPTLWSPSTQHHGSSSGSMSSPLRKSVRCAQMALSPCSSNIQPCDDRDSQGTAEWDSSSTSEDSDFEDSL RRNVRKRAAKRPPKAIPVAKHPKKQSHIVPGGNDKNKSVPPTSDLFDAVKAARSCAQSLVDEWLENYKQD ENAGFLELVNFFIRACGCKSTVTPEMFKTMSNSEIIQHLTEEFNEDSGDYPLTAPGPSWKKFQGSFCEFV KTLVCQCQYSLLFDGFPMDDLISLLIGLSDSQVRAFRHTSTLAAMKLMTSLVKVALQLSLHKDNNQRQYE AERNKGPEQRAPERLESLLEKRKEFQENQEEIEGMMNAIFRGVFVHRYRDILPEIRAVCIEEIGCWMQSY STSFLNDSYLKYIGWTLHDKHKEVRLKCVKALAGLYSNQELSSRMELFTNRFKDRMVSMVMDRESEVAVE AIRLLTLILKNMEGVLTSADCEKIYSIVYISNRAMASSAGEFVYWKIFHPECGAKAVSGRERRRSPQAQR TFIYLLLAFFMESEHHDHAAYLVDSLWDCAGSYLKDWESLTSLLLQKDQNLGDMQERMLIEILVSSARQA AEGHPPVGRITGKKSLTAKERKLQAYDKVKLAEHLIPLLPQLLAKFSADAENVAPLLRLLSYFDLNIYCT QRLEKHLELLLQQLQEVVVKHVEPEVLEAAAHALYLLCKPEFTFFSRVDFARSQLVDLLTDRFQQELDDL MQSSFLDEDEVYSLTATLKRLSAFYNAHDLTRWEISEPCSRLLRKAVDTGEVPHQVILPALTLVYFSILW TVTHISESTSQKQLMSLKKRMVAFCELCQSCLSDVDPEIQEQAFVLLSDLLLIFSPQMVVGGRDFLRPLV FFPEATLQSELASFLMDHVFLQPGELGNGQSQEDHVQIELLHQRRRLLAGFCKLLLYGVLELDAASDVFK HYNKFYEDYGDIIKETLTRARQIDRCQCSRILLLSLKQLYTELIQEQGPQDLTELPAFIEMRDLARRFAL SFGPQQLHNRDLVVMLHKEGIKFSLSELPPAGSSREPPNIAFLELLSEFSPRLFHQDKQLLLSYLEKCLQ RVSMAPSHPWGPVTTYCHSLHLVENTAEASSQGPPHSKKRCIEVPRRLQEEESSSQGESLQLNSGPTTPT LTSTAVKRRQSPRTVGKRQKGGPGPGPGPGPGPGPGPGPGPGPGPGPELICSQQLSGTQRLKMSSAPCFQ IRCDPSGSGLGKQMTRLSLMEEDEEEELRLLDEEWQCGDKLLHSPSSPSEHGLDLLDTTELNMEDF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053730
ORF Size	3768 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053730.1, NP_446182.1
RefSeq Size	4181 bp
RefSeq ORF	3771 bp
Locus ID	114522
UniProt ID	Q99M76
MW	142 kDa
Gene Summary	testis-specific protein which associates with the synaptonemal complex [RGD, Feb 2006]

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