Capn1 (NM_019152) Rat Tagged ORF Clone
CAT#: RR211513
- TrueORF®
Capn1 (Myc-DDK-tagged ORF) - Rat calpain 1 (Capn1), (10 ug)
"NM_019152" in other vectors (3)
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CNY 9,120.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR211513 representing NM_019152
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGAGGAGTTAATCACACCTGTGTACTGCACCGGGGTGTCAGCCCAAGTGCAGAAGCAACGGGACA AGGAGTTGGGCTTGGGCCGCCATGAAAATGCCATCAAGTACCTGGGCCAGGATTATGAAAATCTTCGTGC AAGATGCCTGCAGAACGGGGTCCTCTTCCAAGACGATGCCTTCCCTCCAGTTTCTCATAGCTTGGGCTTC AAGGAACTGGGTCCCAATTCCTCTAAAACCTATGGCATCAAGTGGAAGCGTCCTACGGAACTGCTGTCAA ACCCCCAGTTCATCGTGGATGGAGCCACCCGAACGGACATCTGCCAGGGAGCACTGGGGGACTGTTGGCT CCTGGCTGCCATTGCCTCGCTCACTCTCAACGAGACTATCCTCCACCGAGTGGTTCCCTACGGCCAGAGC TTCCAGGAGGGCTATGCTGGCATCTTTCATTTCCAGCTGTGGCAGTTTGGGGAGTGGGTAGATGTGGTTG TGGATGATTTGCTACCCACCAAGGATGGAAAGCTGGTGTTCGTGCACTCTGCCCAGGGCAACGAGTTCTG GAGCGCTCTCCTGGAGAAAGCCTATGCAAAAGTGAATGGCAGCTATGAGGCCCTTTCGGGAGGCTGCACC TCAGAGGCCTTCGAGGACTTTACCGGTGGGGTCACTGAGTGGTACGATCTGCAGAAGGCACCCAGCGACC TCTACCAGATCATTCTGAAGGCCCTGGAACGAGGCTCCTTGCTGGGCTGCTCCATTAATATCTCCGATAT CCGTGATTTGGAGGCTATTACTTTTAAGAACCTGGTGAGGGGCCATGCATACTCTGTGACGGATGCCAAG CAGGTCACTTACCAGGGCCAGCGGGTGAACCTAATCCGGATGCGGAACCCCTGGGGTGAAGTGGAGTGGA AAGGACCCTGGAGTGACAACTCCTATGAGTGGAACAAAGTGGACCCCTATGAACGTGAGCAGCTGAGGGT CAAGATGGAGGACGGGGAGTTCTGGATGTCCTTCCGGGACTTCATACGTGAGTTCACCAAACTGGAAATC TGCAACCTTACACCTGACGCCCTTAAGAGCAGAACCCTCCGGAACTGGAATACCACATTTTACGAGGGCA CCTGGCGCCGGGGAAGCACTGCTGGAGGCTGCAGGAACTACCCAGCTACCTTCTGGGTAAACCCCCAGTT CAAGATCCGGCTGGAGGAGGTGGATGACGCAGACGACTATGACAGTCGAGAGTCCGGCTGCAGCTTCTTG TTGGCCCTCATGCAGAAGCATCGCCGCAGGGAGCGTCGCTTTGGCCGGGACATGGAGACCATTGGTTTTG CAGTGTACCAGGTCCCTCGGGAGTTGGCGGGTCAGCCTGTGCACTTGAAGCGTGACTTCTTCCTGGCCAA TGCTTCTCGGGCACAATCAGAGCACTTTATCAACCTTCGAGAGGTCAGTAACCGCATCCGCCTGCCGCCG GGGGAGTATATAGTGGTGCCCTCCACCTTCGAGCCCAACAAAGAGGGTGACTTTCTGCTGCGCTTCTTTT CAGAGAAGAAGGCTGGGACCCAGGAACTAGATGACCAGATCCAGGCCAACCTCCCCGATGAGAAAGTGCT CTCTGAAGAGGAGATCGATGACAACTTCAAAACCCTGTTCAGCAAGTTGGCAGGGGATGACATGGAGATC AGTGTCAAGGAGTTACAGACCATCTTGAACAGAATCATTAGCAAACACAAAGACCTGCGCACTAACGGCT TCAGCCTGGAGTCGTGCCGCAGCATGGTGAACCTCATGGATCGAGACGGTAATGGGAAACTGGGCCTGGT GGAGTTCAACATCCTGTGGAACCGCATCCGAAATTACCTGACCATCTTCCGAAAGTTTGACCTGGACAAG TCTGGCAGCATGAGTGCTTATGAGATGAGGATGGCCATCGAGGCTGCAGGCTTCAAGCTTAACAAGAAGC TGCATGAGCTCATCATCACCCGCTACTCAGAGCCCGACCTGGCCGTGGACTTTGACAACTTTGTGTGCTG CCTTGTGCGCCTGGAGACCATGTTCCGGTTTTTCAAAATTCTGGACACAGACCTGGATGGTGTTGTGACC TTTGATCTATTTAAGTGGTTACAGCTGACTATGTTTGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR211513 representing NM_019152
Red=Cloning site Green=Tags(s) MAEELITPVYCTGVSAQVQKQRDKELGLGRHENAIKYLGQDYENLRARCLQNGVLFQDDAFPPVSHSLGF KELGPNSSKTYGIKWKRPTELLSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNETILHRVVPYGQS FQEGYAGIFHFQLWQFGEWVDVVVDDLLPTKDGKLVFVHSAQGNEFWSALLEKAYAKVNGSYEALSGGCT SEAFEDFTGGVTEWYDLQKAPSDLYQIILKALERGSLLGCSINISDIRDLEAITFKNLVRGHAYSVTDAK QVTYQGQRVNLIRMRNPWGEVEWKGPWSDNSYEWNKVDPYEREQLRVKMEDGEFWMSFRDFIREFTKLEI CNLTPDALKSRTLRNWNTTFYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLEEVDDADDYDSRESGCSFL LALMQKHRRRERRFGRDMETIGFAVYQVPRELAGQPVHLKRDFFLANASRAQSEHFINLREVSNRIRLPP GEYIVVPSTFEPNKEGDFLLRFFSEKKAGTQELDDQIQANLPDEKVLSEEEIDDNFKTLFSKLAGDDMEI SVKELQTILNRIISKHKDLRTNGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLTIFRKFDLDK SGSMSAYEMRMAIEAAGFKLNKKLHELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKILDTDLDGVVT FDLFKWLQLTMFA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_019152 |
ORF Size | 2139 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_019152.2, NP_062025.1 |
RefSeq Size | 3020 bp |
RefSeq ORF | 2142 bp |
Locus ID | 29153 |
UniProt ID | P97571 |
MW | 82.1 kDa |
Gene Summary | calcium-dependent protease that may be involved in experimental allergic encephalomyelitis (EAE) [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN211513 | Capn1 (untagged ORF) - Rat calpain 1 (Capn1), (10 ug) |
CNY 9,120.00 |
|
RR211513L3 | Lenti ORF clone of Capn1 (Myc-DDK-tagged ORF) - Rat calpain 1 (Capn1), (10 ug) |
CNY 10,930.00 |
|
RR211513L4 | Lenti ORF clone of Capn1 (mGFP-tagged ORF) - Rat calpain 1 (Capn1), (10 ug) |
CNY 12,070.00 |