Nrcam (NM_013150) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR211835 representing NM_013150 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGAAGAAGAAGCCCTTGTCTGCAGGCAGAGCGCCCCTGTTTCTCTTCCTGTGCCAGATGATCAGCG CTCTGGATGTTCCTCTTGATCCAAAGCTCCTTGATGACTTGGTACAGCCTCCAACTATCACTCAACAGTC ACCAAAAGACTACATCATTGACCCACGGGAGAATATTGTAATCCAATGTGAGGCCAAAGGGAAACCTCCT CCAAGCTTTTCCTGGACTCGTAACGGAACACATTTTGACATAGACAAAGACCCTCTGGTCACTATGAAGC CTGGCTCAGGAACCCTTGTCATCAACATCATGAGTGAAGGAAAGGCGGAGACCTATGAAGGGGTTTACCA GTGCACTGCAAGGAATGAGCGCGGAGCTGCTGTCTCCAATAACATTGTTGTCCGCCCCTCTAGGTCACCC TTGTGGACCAAGGAAAGACTTGAACCAATAATCCTCCGAAGTGGTCAGTCACTAGTACTACCATGTAGGC CTCCAATTGGATTACCACCGGCCATAATATTTTGGATGGATAACTCCTTTCAAAGACTGCCACAGAGTGA GCGGGTTTCCCAAGGACTGAATGGAGACCTTTACTTCTCCAATGTCCTCCCAGAGGACACCCGTGAGGAC TACATCTGCTATGCCAGATTTAATCACACTCAAACAATTCAACAGAAACAACCTATTTCTCTGAAGGTGA TTTCAGTGGATGAATTGAATGACACTATAGCTGCTAATTTGAGTGACACTGAGTTTTATGGTGCTAAATC TAGTAAAGAGAGGCCACCAACATTTCTAACTCCAGAGGGCAATGAAAGTCACAAGGAAGAATTAAGAGGA AACGTGCTTTCCCTGGAGTGCATTGCAGAAGGCCTACCTACTCCAGTTATTTACTGGATCAAGGAAGATG GAACGCTTCCTGTCAACCGGACGTTTTATCGGAACTTTAAGAAAACCTTGCAGATCATTCATGTCTCTGA AGCAGACTCTGGAAATTATCAGTGCATAGCAAAAAACGCATTGGGAGCCGTCCATCATACCATTTCTGTC ACAGTTAAAGCGGCTCCCTACTGGATTGTTGCACCTCACAACCTCGTGCTTTCCCCAGGGGAGAATGGGA CCCTCATCTGCAGAGCTAACGGCAACCCAAAACCCAGAATTAGCTGGTTAACAAATGGAGTCCCAGTAGA AATTGCTCTCGATGACCCCAGCCGAAAAATCGATGGTGATACCATTATGTTTTCAAATGTTCAAGAAAGC TCAAGTGCGGTTTATCAGTGCAATGCCTCTAACAAATATGGATATTTACTAGCAAATGCATTTGTAAATG TGCTCGCTGAACCACCTCGGATTCTTACCTCAGCAAACACACTGTACCAGGTCATTGCAAACCGGCCTGC TTTGCTGGACTGTGCCTTCTTTGGGTCTCCTATGCCTACCATTGAGTGGTTTAAAGGAACTAAAGGAAGC GCTCTTCACGAAGACATTTATGTTTTACATGATAACGGAACGCTAGAAATTCCTGTGGCCCAAAAGGATA GTACAGGGACATATACTTGTGTCGCAAGGAATAAATTAGGGATGGCAAAGAATGAAGTTCACTTGGAAAT CAAAGATCCAACCAGGTTCATTAAACAACCTGAATATGCAGTCGTTCAGAGGGGGAGCAAGGTTTCCTTT GAATGCAAAGTGAAACACGACCACACCTTAATCCCCACCATTCTGTGGCTGAAGGACAATGGAGAGCTGC CCAATGATGAAAGGTTCTCTGTCGACAAGGACCATCTGGTGGTCTCTGACGTAAAGGATGAAGATGGTGG GACCTACACATGTGCAGCCAACACAACGCTGGACAGTGTTTCAGCCAGCGCCGTGCTCAGGGTGGTCGCT CCTACTCCAACTCCAGCCCCCATTTACGATGTCCCGAATCCTCCCTTTGATTTAGAATTGACCAATCAAC TTGACAAAAGTGTTCAGCTGACATGGACCCCAGGCGATGACAACAATAGTCCCATTACAAAATTCATCAT CGAGTATGAAGATGCAATGCATGAGGCAGGGCTGTGGCGCCACCAGGCGGAAGTTTCTGGAACGCAGACC ACAGCCCAGCTGAAGCTGTCTCCCTATGTGAACTACTCCTTCCGAGTCATGGCGGAGAACAGCATCGGGA GGAGTGTGCCAAGCGAGGCATCGGAGCAGTATCTTACAAAGGCCGCGGAACCAGACCAGAATCCCACGGC TGTGGAAGGACTAGGGACAGAGCCGGACAACTTGGTGATCACATGGAAGCCCCTGAATGGTTTTCAATCG AATGGGCCCGGCCTCCAGTACAAAGTGAGCTGGCGTCAGAAAGATGGTGACGATGAATGGACGTCTGTGG TTGTGGCCAATGTATCCAAATACATTGTTTCTGGCACACCAACTTTTGTCCCATACCTGATAAAAGTTCA AGCCCTGAATGATGTGGGCTTTGCTCCAGAGCCAGCTGCAGTCATGGGACATTCCGGAGAAGACCTTCCA ATGGTGGCTCCTGGAAATGTGCGTGTCAGTGTGGTGAACAGCACCTTGGCAGAGGCACACTGGGACCCTG TACCTCCCAAGAGTGTCCGAGGACACTTACAAGGCTACCGGATTTACTACTGGAAGGCTCAGAGCTCATC TAAAAGAAACAGACGCCACATTGAGAAGAAGATCCTCACCTTCCAGGGCAGCAAGACTCACGGCATGCTG CCAGGGCTGCAACCTTACAGTCACTATGTTCTCAATGTCCGAGTGGTCAATGGGAAAGGGGAGGGCCCAG CCAGCGCCGACAGAGGCTTCCATACTCCAGAGGGAGTCCCTAGTGCTCCCTCATCTTTGAAAATTGTTAA TCCTACACTGGACTCTCTCACTTTGGAATGGGACCCTCCAAGCCACCCAAATGGTATCCTGACTGAATAC ATCTTAAAATATCAGCCAATTAATAGCACACACGAACTAGGCCCTCTGGTAGATTTAAAAATTCCTGCCA ACAAGACACGCTGGACTTTGAAAAATTTAAATTTCAGCACTCGGTACAAGTTCTACTTCTATGCACAAAC ATCAGTGGGATCGGGCAGTCAGATCACAGAGGAAGCAATAACAACTGTGGACGAAGCTGGTATTCTTCCA CCTGATGTAGGTGCAGGCAAAGTCCGTGCAGTAAGTCCCAGGATCGGCAATGTCACTGCTGCAGCTGCTG AGACGTATGCCAATATCAGTTGGGAATATGAGGGACCAGAGCATGTGAAGTTTTATGTTGAATATGGTGT AGCAGGCAGCAAAGAAGAATGGAGGAAAGAGATTGTAAATGGTTCTCGGAGCTTCTTTGGGTTAAAGGGT CTAATGCCAGGAACAGCATACAAAGTCCGAGTTGGTGCTGAGGGGGACTCTGGTTTTGTGAGTTCAGAGG ATGTGTTTGAGACAGGACCAGCAATGGCAAGTCGGCAAGTGGATATTGCGACCCAAGGCTGGTTCATAGG TCTAATGTGTGCTGTTGCCCTCCTCATCTTAATTTTGCTAATCGTTTGCTTCATCAGAAGAAACAAAGGT GGTAAATATCCAGTTAAAGAAAAGGAGGATGCTCACGCAGACCCTGAAATCCAGCCCATGAAGGAGGATG ACGGAACGTTTGGAGAATATAGTGATGCAGAAGATCACAAGCCTTTGAAAAAAGGAAGTCGAACACCTTC AGACAGGACTGTGAAAAAAGAAGATAGTGATGACAGCCTGGTTGACTATGGCGAGGGGGTGAATGGCCAA TTCAATGAGGATGGCTCCTTTATCGGACAATACAGTGGTAAGAAAGAGAAAGAACCAGCAGAGGGAAATG AAAGCTCAGAGGCCCCTTCTCCTGTCAACGCAATGAACTCCTTTGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR211835 representing NM_013150 Red=Cloning site Green=Tags(s) MPKKKPLSAGRAPLFLFLCQMISALDVPLDPKLLDDLVQPPTITQQSPKDYIIDPRENIVIQCEAKGKPP PSFSWTRNGTHFDIDKDPLVTMKPGSGTLVINIMSEGKAETYEGVYQCTARNERGAAVSNNIVVRPSRSP LWTKERLEPIILRSGQSLVLPCRPPIGLPPAIIFWMDNSFQRLPQSERVSQGLNGDLYFSNVLPEDTRED YICYARFNHTQTIQQKQPISLKVISVDELNDTIAANLSDTEFYGAKSSKERPPTFLTPEGNESHKEELRG NVLSLECIAEGLPTPVIYWIKEDGTLPVNRTFYRNFKKTLQIIHVSEADSGNYQCIAKNALGAVHHTISV TVKAAPYWIVAPHNLVLSPGENGTLICRANGNPKPRISWLTNGVPVEIALDDPSRKIDGDTIMFSNVQES SSAVYQCNASNKYGYLLANAFVNVLAEPPRILTSANTLYQVIANRPALLDCAFFGSPMPTIEWFKGTKGS ALHEDIYVLHDNGTLEIPVAQKDSTGTYTCVARNKLGMAKNEVHLEIKDPTRFIKQPEYAVVQRGSKVSF ECKVKHDHTLIPTILWLKDNGELPNDERFSVDKDHLVVSDVKDEDGGTYTCAANTTLDSVSASAVLRVVA PTPTPAPIYDVPNPPFDLELTNQLDKSVQLTWTPGDDNNSPITKFIIEYEDAMHEAGLWRHQAEVSGTQT TAQLKLSPYVNYSFRVMAENSIGRSVPSEASEQYLTKAAEPDQNPTAVEGLGTEPDNLVITWKPLNGFQS NGPGLQYKVSWRQKDGDDEWTSVVVANVSKYIVSGTPTFVPYLIKVQALNDVGFAPEPAAVMGHSGEDLP MVAPGNVRVSVVNSTLAEAHWDPVPPKSVRGHLQGYRIYYWKAQSSSKRNRRHIEKKILTFQGSKTHGML PGLQPYSHYVLNVRVVNGKGEGPASADRGFHTPEGVPSAPSSLKIVNPTLDSLTLEWDPPSHPNGILTEY ILKYQPINSTHELGPLVDLKIPANKTRWTLKNLNFSTRYKFYFYAQTSVGSGSQITEEAITTVDEAGILP PDVGAGKVRAVSPRIGNVTAAAAETYANISWEYEGPEHVKFYVEYGVAGSKEEWRKEIVNGSRSFFGLKG LMPGTAYKVRVGAEGDSGFVSSEDVFETGPAMASRQVDIATQGWFIGLMCAVALLILILLIVCFIRRNKG GKYPVKEKEDAHADPEIQPMKEDDGTFGEYSDAEDHKPLKKGSRTPSDRTVKKEDSDDSLVDYGEGVNGQ FNEDGSFIGQYSGKKEKEPAEGNESSEAPSPVNAMNSFV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_013150
ORF Size	3897 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_013150.1, NP_037282.1
RefSeq Size	7556 bp
RefSeq ORF	3900 bp
Locus ID	497815
MW	143.1 kDa
Gene Summary	a neuronal cell adhesion molecule localized at the node of Ranvier of myelinated axons [RGD, Feb 2006]

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