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Fbxo7 (NM_001012222) Rat Tagged ORF Clone
CAT＃: RR211901
- TrueORF®
Fbxo7 (Myc-DDK-tagged ORF) - Rat F-box protein 7 (Fbxo7), (10 ug)
"NM_001012222" in other vectors (3)
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CNY 6,750.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR211901 representing NM_001012222
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCTGCGCGTGCGGCTTCAAAAGCGGACCCAGCCGCTCGAGGTGCCGGAGTCGGAGCCGACGCTCG GGCAGCTGCGCGCGCACCTCATCCAGGACCTGCTGCCCACGTTGGGGTTCAGTTCTGATACCCGATTTGC AATTACATTGAACAACAAGGATGCCCTCACTGGAGATGAAGAGACCTTGGCTTCATATGGGATTGTTTCT GGGGACTTGATATGTTTGGTTCTTGAAGATGAAATGCCGGCACCTAACTTACCTTCATCTACAGATTCAG AGCATTCCTCTCTCCAGAATAATGACCAACCTCCTTTGGCTGCCACCTCAAGCCAGGCTAACATCCCAGA TGAACAAGGGAGTGATTCATCCCACGGACAGGTCACCCAATATGATGCCTGGACCGATGACAGTATGGAA GGACCTAGTCACAGTGCTGAAGCTGTGTCAATTCAGGACGCCATGAGTGTGGAAGAGGCTTCTGGTTTCC ATCCACTGGAGCCAATGCTCTGCAGTGAAACAGAGGACGGGCAGGTGCCACATTCCCTAGAGGCCCTGTA CCAGTCAGCCGGCTGCTCCACCGTCAGTGATGCCTTGATAGTGCTGGTGCATCTCCTTATGCTTGAGTCG GGCTACATACCTCAGGGGACTGAAGCCAAAGCAGCATCCATGCCTGAGAAATGGAAGTCAAGTGGCGTAT ACAAGCTCCAGTACACACATCCTCTCTGTGAGGGCGGCTCTGCCGTGCTTACCTGTGTACCTTTGGGAAA GCTCATCATGATAAACGCTACAATAAAAGTCAACGGTGGGATTAAAAATGTGAAGTCAGTGCAGCTGAAG CCAGGAGCCTACGTTCGCAGAGCAGAACCAGGGGAAAGTGCAGCTAAAGTGTACAAAGATCTTAAGAAGC TCTCTCGTCTCTTCAAAGACCAGCTGGTGTACCCTCTTCTGGCTTTCACACGACAAGTGCTGAACCTCCC AGATGTGTTTGGGCTGGTTGTCCTTCCACTGGAGCTGAAACTACGCATCTTCCGACTTCTGGATGTTCAT TCCGTCCTGGCTCTGTCTGCAGTCTGCCATGACCTCCTCATTGCATCAAATGACCCACTGCTGTGGAGGT GCTTGTATCTGCGGGATTTTCGAGACAGTACTATCAGAGGTCCTGACACAGATTGGAAAGAATTGTACAG AAAGAAGCACATCCAAAGAAAAGAAGCCCAGAGGATGCGGCATGTGATGTACCTACCGTCAGTCCACCCT ATCCCATTCTGCCCCATTCCCGTCTACCCCAGGCCCTACCTTCCCACCACGCTGCTTCCTCCAGGAATCA TTGGTGGTGAATATGATGAGAGGCCAATATTGCCTAGTGTTGGGGACCCAGTCACCTCACTCATCCCAAG GCCTGGGGAGCCTCCTAGCCAGTTCCGTCCAGTCAGACCACGTTTTGATCCTGTTGGCCCACTTCCAGGA TCTAACTCCCTCTTACCAGGAAGAGCTAGCCCCAACAACAGATTTCCCTTCAGACCCGGCAGGGGTCGGT CAGCTGACAACCGGCTACCATACCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR211901 representing NM_001012222
Red=Cloning site Green=Tags(s) MKLRVRLQKRTQPLEVPESEPTLGQLRAHLIQDLLPTLGFSSDTRFAITLNNKDALTGDEETLASYGIVS GDLICLVLEDEMPAPNLPSSTDSEHSSLQNNDQPPLAATSSQANIPDEQGSDSSHGQVTQYDAWTDDSME GPSHSAEAVSIQDAMSVEEASGFHPLEPMLCSETEDGQVPHSLEALYQSAGCSTVSDALIVLVHLLMLES GYIPQGTEAKAASMPEKWKSSGVYKLQYTHPLCEGGSAVLTCVPLGKLIMINATIKVNGGIKNVKSVQLK PGAYVRRAEPGESAAKVYKDLKKLSRLFKDQLVYPLLAFTRQVLNLPDVFGLVVLPLELKLRIFRLLDVH SVLALSAVCHDLLIASNDPLLWRCLYLRDFRDSTIRGPDTDWKELYRKKHIQRKEAQRMRHVMYLPSVHP IPFCPIPVYPRPYLPTTLLPPGIIGGEYDERPILPSVGDPVTSLIPRPGEPPSQFRPVRPRFDPVGPLPG SNSLLPGRASPNNRFPFRPGRGRSADNRLPYL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001012222 |
| ORF Size | 1566 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001012222.1, NP_001012222.1 |
| RefSeq Size | 1819 bp |
| RefSeq ORF | 1569 bp |
| Locus ID | 366854 |
| UniProt ID | Q68FS3 |
| MW | 57.6 kDa |
| Gene Summary | Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes BIRC2 and DLGAP5. Plays a role downstream of PINK1 in the clearance of damaged mitochondria via selective autophagy (mitophagy) by targeting PRKN to dysfunctional depolarized mitochondria. Promotes MFN1 ubiquitination (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN211901 | Fbxo7 (untagged ORF) - Rat F-box protein 7 (Fbxo7), (10 ug) |
CNY 6,750.00 |
|
| RR211901L3 | Lenti ORF clone of Fbxo7 (Myc-DDK-tagged ORF) - Rat F-box protein 7 (Fbxo7), (10 ug) |
CNY 8,650.00 |
|
| RR211901L4 | Lenti ORF clone of Fbxo7 (mGFP-tagged ORF) - Rat F-box protein 7 (Fbxo7), (10 ug) |
CNY 9,500.00 |
