Abca2 (NM_024396) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Abc2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR212636 representing NM_024396 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTTCCTGCACCAGCTGCAGCTGCTGCTCTGGAAGAACGTGACGCTGAAGCGCCGGAGCCCGTGGG TCCTGGCGTTTGAGATCTTCATCCCCCTTGTCCTCTTCTTCATCCTGTTGGGACTGCGGCAGAAGAAGCC CACCATCTCTGTGAAGGAAGCTTTCTACACGGCAGCACCGCTGACATCGGCCGGCATCCTGCCTGTCATG CAGTCGCTTTGCCCTGATGGCCAGCGTGATGAGTTTGGCTTCCTGCAGTATGCCAACTCCACGGTCACCC AGCTTCTGGAACGCCTCAACCGTGTAGTGGAAGAGAGCAACTTGTTTGACCCAGAGCGACCTAGCCTGGG CTCAGAGCTTGAGGCACTGCACCAACGTCTGGAGGCCCTCAGCTCGGGCCCTGGCACCTGGGAGAGCCAC TCAGCTAGACCTGCAGTTTCATCCTTCTCTCTGGACTCGGTGGCCAGGGACAAAAGAGAGCTTTGGCGTT TCCTGATGCAGAACCTGTCACTGCCCAACAGCACGGCCCAGGCCCTCCTGGCTGCCCGTGTAGACCCTTC TGAGGTCTATCGCTTGCTTTTTGGTCCTTTACCTGACCTGGATGGAAAGTTGGGGTTCCTCAGGAAGCAG GAGCCCTGGAGTCACCTGGGTAGCAATCCTCTGTTCCAAATGGAGGAGCTGCTGCTGGCTCCTGCCCTTT TGGAGCAACTCACATGTGCTCCAGGCTCTGGGGAGCTGGGCCGGATTCTTACCATGCCTGAGGGTCATCA GGTAGACCTTCAGGGCTACCGGGATGCTGTCTGCAGCGGGCAGGCTACAGCTCGTGCCCAGCATTTCAGT GATCTAGCCACTGAGCTCCGGAACCAGCTGGACATAGCCAAGATTGCCCAGCAGCTGGGCTTCAATGTCC CCAACGGCTCAGATCCACAGCCGCAGGCACCGTCCCCACAGAGTCTGCAGGCACTCTTAGGGGACCTGCT GGATGTCCAGAAGGTTCTACAGGATGTGGATGTCCTATCAGCCCTTGCCCTGCTGCTGCCTCAAGGTGCC TGTGCTGGCCGGGCCCCCGCACCTCAAGCTGGCAGCCCGAGTGGCCCGGCCAACAGCACCGGGGTAGGGG CAAATACAGGTCCCAACACCACCGTTGAGGAGGGCACCCAGTCACCTGTCACCCCAGCCTCTCCGGACAC TCTGCAAGGCCAGTGCTCAGCCTTTGTGCAGCTCTGGGCTGGCTTGCAGCCCATCTTGTGTGGCAACAAC CGTACCATTGAGCCTGAAGCACTCCGGAGGGGCAACATGAGCTCACTGGGCTTTACGAGCAAAGAACAAC GGAACCTGGGCCTTCTTGTGCACCTCATGACCAGCAACCCCAAAATCCTGTATGCACCCGCAGGCTCTGA AGCTGACCATGTTATCCTCAAGGCAAATGAGACCTTTGCCTTTGTGGGCAACGTGACGCACTACGCCCAG GTCTGGCTCAACATCTCCGCAGAGATCCGGAGCTTCCTGGAGCAGGGCAGGCTGCAGCAGCATCTGCACT GGCTGCAGCAGTACGTGGCTGACCTCCGGCTACACCCTGAAGCAATGAACCTGTCACTGGACGAGCTGCC CCCTGCTCTGCGCCTGGACTACTTTTCTCTGCCCAATGGCACAGCCCTTCTGCAGCAGCTAGACACAATA GACAATGCAGCCTGTGGCTGGATCCAGTTCATGTCCAAGGTGAGTGTGGACATCTTCAAGGGGTTTCCTG ATGAGGAGAGCATCGTGAACTACACTCTCAATCAGGCCTACCAGGACAATGTTACAGTATTTGCCAGCGT GATTTTCCAGACACGGAAGGATGGTTCCCTCCCCCCACATGTCCATTACAAGATTCGCCAGAACTCAAGC TTCACCGAGAAAACCAACGAGATCCGTCGTGCTTACTGGCGTCCAGGGCCCAACACTGGTGGCCGCTTCT ACTTCCTCTACGGCTTCGTCTGGATCCAGGACATGATAGAACGTGCCATCATCAACACGTTTGTGGGGCA CGACGTGGTCGAACCCGGCAACTACGTGCAGATGTTCCCGTACCCCTGCTACACCCGTGACGACTTCCTG TTTGTCATTGAGCACATGATGCCACTGTGCATGGTGATCTCCTGGGTTTACTCTGTGGCCATGACCATAC AGCACATCGTGGCGGAGAAAGAGCATCGGCTAAAGGAGGTGATGAAGACGATGGGCCTGAACAACGCCGT GCACTGGGTGGCCTGGTTCATCACGGGCTTTGTGCAGCTGTCCATCTCCGTGACAGCCCTGACCGCCATC CTCAAGTATGGCCAGGTCCTCATGCACAGCCACGTGCTCATCATATGGCTCTTCCTTGCTGTCTATGCTG TGGCCACTATCATGTTCTGCTTCCTGGTGTCTGTGCTGTACTCTAAGGCCAAGTTGGCCTCGGCCTGCGG CGGCATCATCTACTTCCTGAGCTACGTTCCCTACATGTATGTAGCAATCCGTGAGGAAGTAGCCCACGAT AAGATCACTGCCTTCGAGAAGTGCATTGCGTCCCTGATGTCCACAACAGCCTTCGGCCTGGGTTCCAAGT ACTTTGCTCTGTATGAAGTGGCAGGTGTGGGCATCCAGTGGCACACGTTCAGCCAGTCCCCAGTGGAAGG GGATGACTTCAACCTGCTCCTTGCTGTCACCATGCTGATGGTGGACACAGTGGTCTATGGCGTACTCACT TGGTACATTGAGGCTGTGCACCCAGGTATGTATGGGCTGCCCCGGCCCTGGTACTTCCCACTACAGAAGT CCTATTGGCTGGGCAGTGGGCGGACAGAGACCTGGGAGTGGAGCTGGCCATGGGCACACGCACCACGCCT CAGCGTTATGGAGGAGGACCAGGCCTGTGCCATGGAGAGCCGGCACTTCGAGGAGACTCGCGGTATGGAG GAGGAGCCCACCCACCTGCCTTTGGTCGTCTGCGTGGACAAGCTCACCAAGGTCTATAAAAATGACAAGA AGCTGGCCTTAAACAAACTGAGCCTCAATCTCTACGAGAACCAGGTGGTCTCTTTCCTAGGCCATAACGG GGCTGGCAAGACCACTACCATGTCTATCCTGACTGGACTGTTCCCACCCACGTCGGGCTCAGCCACTATC TATGGCCACGACATCCGCACAGAGATGGATGAGATCCGTAAGAACCTGGGCATGTGCCCACAGCACAACG TGCTCTTTGACCAGCTCACTGTGGAGGAACACCTCTGGTTCTACTCACGCCTCAAAAGCATGGCACAAGA GGAGATCCGCAAAGAGATGGACAAGATGATCGAGGACCTGGAGCTCTCCAACAAGCGCCACTCGCTGGTA CAGACGCTGTCTGGAGGCATGAAGCGCAAGCTTTCAGTAGCCATTGCCTTCGTGGGTGGCTCTAGAGCCA TTATCTTAGATGAGCCCACAGCTGGCGTGGACCCCTATGCTCGACGTGCCATCTGGGACCTCATTCTGAA GTACAAGCCGGGTCGCACTATCCTCCTGTCCACCCATCACATGGATGAGGCCGACCTGCTGGGGGACCGC ATTGCCATCATCTCCCATGGGAAGCTCAAATGCTGCGGCTCTCCCCTCTTCCTCAAGGGTGCCTACGGCG ATGGCTACCGCCTCACACTGGTCAAGCGGCCTGCGGAGCCTGGCACCTCCCAAGAGCCAGGGATGGCTTC CAGCCCCTCAGGTCGTCCTCAGCTGAGCAACTGCTCAGAGATGCAAGTGTCCCAGTTCATCCGCAAGCAT GTGGCTTCCTCCCTGCTGGTGTCAGACACGAGCACCGAGCTCTCCTACATCCTGCCCAGCGAGGCTGTCA AGAAAGGGGCCTTCGAGCGCCTCTTTCAGCAATTGGAGCACAGCCTGGACGCACTGCATCTGAGCAGTTT TGGGCTGATGGACACAACCCTGGAGGAGGTGTTCCTCAAGGTGTCTGAAGAAGACCAGTCACTGGAGAAT AGTGAGGCCGATGTGAAGGAGTCCCGGAAGGATGCACTGCCTGGGGCAGAGGGCCTGACGGCTGTGGAGA GTCAAGCAGGCAACCTGGCTCGGTGCTCAGAGCTGGCACAGTCCCAGGCTTCACTGCAGTCTGCATCTTC TGTGGGCTCCGCCCGTGGGGATGAGGGTGCTGGCTACACCGACGGCTACGGTGACTACCGTCCCCTCTTT GACAACTTGCAGGACCCAGACAGTGTCAGCTTACAAGAGGCTGAAATGGAGGCCCTGGCTCGGGTAGGCC AGGGCAGCCGCAAGCTAGAGGGCTGGTGGCTGAAGATGCGGCAGTTCCATGGGCTCCTGGTGAAGCGCTT CCACTGTGCTCGCCGGAACTCCAAAGCGCTCTGCTCCCAGATTCTGCTGCCTGCCTTCTTCGTCTGTGTG GCCATGACTGTGGCATTGTCTGTCCCTGAGATCGGTGACCTGCCTCCACTGGTCCTGTCGCCTTCTCAGT ACCACAACTATACCCAGCCCCGTGGCAACTTTATCCCCTATGCCAATGAGGAACGCCGCGAGTACCGATT ACGGCTGTCACCTGATGCCAGCCCCCAGCAGTTGGTGAGCACATTCCGGCTGCCCTCTGGTGTGGGTGCC ACTTGTGTGCTCAAGTCTCCAGCCAACGGCTCCCTGGGGCCCATGCTGAACTTGAGCAGTGGAGAGTCCC GCCTGCTGGCCGCACGGTTCTTCGACAGTATGTGCCTGGAGTCCTTCACACAGGGGCTGCCACTGTCCAA CTTCGTGCCACCCCCACCCTCGCCCGCCCCTTCCGACTCACCCCTGTCCCCGGATGAGGATTCACTGCTA GCCTGGAATACGTCCCTGCCCCCTACTGCTGGACCAGAGACGTGGACGTGGGCGCCTTCTCTGCCACGCC TGGTTCACGAGCCGGTCCGCTGTACCTGCTCTGCACAGGGCACGGGCTTCTCGTGCCCCAGCAGTGTGGG TGGGCACCCACCCCAGATGAGAGTGGTCACGGGGGACATCCTGACTGACATCACCGGCCACAATGTTTCC GAGTACCTGCTCTTCACCTCTGACCGTTTCCGACTGCACCGCTATGGAGCCATCACCTTTGGTAATATCC AGAAGTCCATCCCAGCACCCATTGGTACCCGGACCCCTCTCATGGTCCGGAAGATTGCAGTGCGGAGGGT GGCCCAGGTGCTCTACAACAACAAGGGCTACCACAGCATGCCCACCTACCTCAACAGCCTCAACAATGCC ATTCTGCGTGCAAACCTACCCAAAAGCAAGGGCAATCCAGCAGCCTACGGTATCACCGTCACCAACCACC CCATGAACAAGACCAGTGCTAGCCTCTCCCTGGATTACCTACTGCAGGGCACAGACGTGGTCATCGCCAT CTTCATCATTGTGGCCATGTCCTTCGTGCCGGCCAGCTTCGTGGTCTTCCTTGTGGCCGAGAAATCCACC AAGGCCAAACACCTGCAGTTCGTCAGCGGGTGCAACCCTGTCATCTACTGGCTAGCCAACTACGTGTGGG ACATGCTCAATTACCTGGTCCCGGCCACCTGCTGCATCATCATCCTCTTCGTGTTTGACTTGCCGGCCTA CACGTCACCCACCAACTTCCCCGCGGTGCTCTCCTTGTTCCTGCTCTATGGATGGTCCATCACACCCATC ATGTACCCGGCCTCCTTCTGGTTTGAGGTCCCTAGCTCAGCCTACGTGTTCCTCATCGTCATCAACCTCT TCATTGGCATCACGGCCACAGTGGCCACCTTCCTTCTGCAGCTCTTTGAGCATGACAAGGATCTGAAGGT TGTCAACAGTTACCTGAAAAGCTGCTTCCTCATCTTCCCCAACTACAACCTGGGCCACGGACTCATGGAG ATAGCCTACAACGAATACATCAACGAATACTATGCCAAGATCGGCCAGTTTGACAAGATGAAGTCCCCGT TTGAGTGGGACATTGTCACACGTGGACTGGTGGCCATGACAGTCGAGGGCTTCGTGGGATTCTTTCTCAC CATCATGTGTCAATATAACTTCCTACGGCAGCCACAGCGTCTGCCTGTGTCTACTAAACCTGTGGAAGAC GATGTAGACGTGGCCAGTGAGCGGCAAAGAGTGCTCCGTGGCGATGCTGACAATGACATGGTCAAGATCG AGAACCTGACTAAGGTGTACAAGTCTCGGAAGATCGGCCGCATCCTGGCAGTGGACCGCCTTTGCCTGGG TGTGCGCCCCGGAGAGTGCTTTGGGCTCCTCGGGGTCAATGGTGCCGGGAAGACCAGCACCTTCAAGATG TTGACTGGAGATGAGAGCACAACAGGGGGCGAGGCCTTTGTCAATGGACACAGTGTGCTCAAGGACCTGC TCCAGGTTCAGCAGAGCCTTGGCTACTGCCCACAGTTCGACGCCCTGTTCGATGAGCTCACGGCTCGCGA ACACCTGCAGCTGTATACTCGGCTTCGAGGCATCCCCTGGAAGGATGAGGCGCAGGTGGTGAGGTGGGCC CTGGAGAAGCTGGAGCTGACGAAGTGTGCAGACAAGCCAGCCGGTAGCTACAGTGGGGGCAACAAACGGA AACTTTCCACAGCCATCGCTCTCATTGGGTACCCTGCCTTCATCTTTCTAGACGAGCCCACCACTGGCAT GGACCCTAAGGCCCGGCGCTTCCTGTGGAACCTCATTCTGGACCTCATCAAGACAGGACGTTCAGTGGTG CTGACCTCACACAGCATGGAGGAATGCGAGGCTGTGTGCACACGGCTGGCCATCATGGTGAATGGACGGC TGCGCTGCCTGGGGAGTATCCAGCACCTCAAGAACAGGTTTGGGGACGGCTACATGATCACTGTAAGGAC CAAAAGCAGCCAGAACGTGAAGGATGTGGTGCGGTTCTTCAACCGGAACTTCCCAGAGGCCATGCTCAAG GAACGCCACCATACGAAGGTGCAGTATCAGCTCAAGTCGGAGCACATCTCGCTGGCTCAGGTGTTCAGCA AGATGGAGCACGTGGTCGGTGTACTGGGCATCGAGGACTACTCAGTCAGCCAGACCACTCTGGATAACGT GTTTGTGAACTTCGCCAAGAAGCAAAGTGACAATGTGGAGCAGCAAGAGGCTGAGCCATCCACCTTGCCG TCCCCCCTTGGACTACTTAGCCTGCTGCGGCCCCGCCCTGCACCCACAGAGCTCCGGGCACTGGTGGCCG ATGAGCCTGAGGACCTGGACACGGAGGACGAGGGCCTCATCAGCTTCGAGGAAGAGCGGGCCCAGCTCTC CTTCAACACCGATACGCTCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR212636 representing NM_024396 Red=Cloning site Green=Tags(s) MGFLHQLQLLLWKNVTLKRRSPWVLAFEIFIPLVLFFILLGLRQKKPTISVKEAFYTAAPLTSAGILPVM QSLCPDGQRDEFGFLQYANSTVTQLLERLNRVVEESNLFDPERPSLGSELEALHQRLEALSSGPGTWESH SARPAVSSFSLDSVARDKRELWRFLMQNLSLPNSTAQALLAARVDPSEVYRLLFGPLPDLDGKLGFLRKQ EPWSHLGSNPLFQMEELLLAPALLEQLTCAPGSGELGRILTMPEGHQVDLQGYRDAVCSGQATARAQHFS DLATELRNQLDIAKIAQQLGFNVPNGSDPQPQAPSPQSLQALLGDLLDVQKVLQDVDVLSALALLLPQGA CAGRAPAPQAGSPSGPANSTGVGANTGPNTTVEEGTQSPVTPASPDTLQGQCSAFVQLWAGLQPILCGNN RTIEPEALRRGNMSSLGFTSKEQRNLGLLVHLMTSNPKILYAPAGSEADHVILKANETFAFVGNVTHYAQ VWLNISAEIRSFLEQGRLQQHLHWLQQYVADLRLHPEAMNLSLDELPPALRLDYFSLPNGTALLQQLDTI DNAACGWIQFMSKVSVDIFKGFPDEESIVNYTLNQAYQDNVTVFASVIFQTRKDGSLPPHVHYKIRQNSS FTEKTNEIRRAYWRPGPNTGGRFYFLYGFVWIQDMIERAIINTFVGHDVVEPGNYVQMFPYPCYTRDDFL FVIEHMMPLCMVISWVYSVAMTIQHIVAEKEHRLKEVMKTMGLNNAVHWVAWFITGFVQLSISVTALTAI LKYGQVLMHSHVLIIWLFLAVYAVATIMFCFLVSVLYSKAKLASACGGIIYFLSYVPYMYVAIREEVAHD KITAFEKCIASLMSTTAFGLGSKYFALYEVAGVGIQWHTFSQSPVEGDDFNLLLAVTMLMVDTVVYGVLT WYIEAVHPGMYGLPRPWYFPLQKSYWLGSGRTETWEWSWPWAHAPRLSVMEEDQACAMESRHFEETRGME EEPTHLPLVVCVDKLTKVYKNDKKLALNKLSLNLYENQVVSFLGHNGAGKTTTMSILTGLFPPTSGSATI YGHDIRTEMDEIRKNLGMCPQHNVLFDQLTVEEHLWFYSRLKSMAQEEIRKEMDKMIEDLELSNKRHSLV QTLSGGMKRKLSVAIAFVGGSRAIILDEPTAGVDPYARRAIWDLILKYKPGRTILLSTHHMDEADLLGDR IAIISHGKLKCCGSPLFLKGAYGDGYRLTLVKRPAEPGTSQEPGMASSPSGRPQLSNCSEMQVSQFIRKH VASSLLVSDTSTELSYILPSEAVKKGAFERLFQQLEHSLDALHLSSFGLMDTTLEEVFLKVSEEDQSLEN SEADVKESRKDALPGAEGLTAVESQAGNLARCSELAQSQASLQSASSVGSARGDEGAGYTDGYGDYRPLF DNLQDPDSVSLQEAEMEALARVGQGSRKLEGWWLKMRQFHGLLVKRFHCARRNSKALCSQILLPAFFVCV AMTVALSVPEIGDLPPLVLSPSQYHNYTQPRGNFIPYANEERREYRLRLSPDASPQQLVSTFRLPSGVGA TCVLKSPANGSLGPMLNLSSGESRLLAARFFDSMCLESFTQGLPLSNFVPPPPSPAPSDSPLSPDEDSLL AWNTSLPPTAGPETWTWAPSLPRLVHEPVRCTCSAQGTGFSCPSSVGGHPPQMRVVTGDILTDITGHNVS EYLLFTSDRFRLHRYGAITFGNIQKSIPAPIGTRTPLMVRKIAVRRVAQVLYNNKGYHSMPTYLNSLNNA ILRANLPKSKGNPAAYGITVTNHPMNKTSASLSLDYLLQGTDVVIAIFIIVAMSFVPASFVVFLVAEKST KAKHLQFVSGCNPVIYWLANYVWDMLNYLVPATCCIIILFVFDLPAYTSPTNFPAVLSLFLLYGWSITPI MYPASFWFEVPSSAYVFLIVINLFIGITATVATFLLQLFEHDKDLKVVNSYLKSCFLIFPNYNLGHGLME IAYNEYINEYYAKIGQFDKMKSPFEWDIVTRGLVAMTVEGFVGFFLTIMCQYNFLRQPQRLPVSTKPVED DVDVASERQRVLRGDADNDMVKIENLTKVYKSRKIGRILAVDRLCLGVRPGECFGLLGVNGAGKTSTFKM LTGDESTTGGEAFVNGHSVLKDLLQVQQSLGYCPQFDALFDELTAREHLQLYTRLRGIPWKDEAQVVRWA LEKLELTKCADKPAGSYSGGNKRKLSTAIALIGYPAFIFLDEPTTGMDPKARRFLWNLILDLIKTGRSVV LTSHSMEECEAVCTRLAIMVNGRLRCLGSIQHLKNRFGDGYMITVRTKSSQNVKDVVRFFNRNFPEAMLK ERHHTKVQYQLKSEHISLAQVFSKMEHVVGVLGIEDYSVSQTTLDNVFVNFAKKQSDNVEQQEAEPSTLP SPLGLLSLLRPRPAPTELRALVADEPEDLDTEDEGLISFEEERAQLSFNTDTLC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_024396
ORF Size	7302 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_024396.1, NP_077372.1
RefSeq Size	8040 bp
RefSeq ORF	7305 bp
Locus ID	79248
UniProt ID	Q9ESR9
MW	270.9 kDa
Gene Summary	glycosylated ABC transporter that binds ATP in the presence of magnesium; may play an important role in brain function [RGD, Feb 2006]

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