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Usp30 (NM_001107153) Rat Tagged ORF Clone
CAT＃: RR213092
- TrueORF®
Usp30 (Myc-DDK-tagged ORF) - Rat ubiquitin specific peptidase 30 (Usp30), (10 ug)
"NM_001107153" in other vectors (3)
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CNY 5,776.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR213092 representing NM_001107153
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGAGTTCCCGGGCGCAGGCGGCGAGGACAGCGGCCGACAAGGCCCTCCAGCGCTTCCTGCGCACCG GGGCGGCTGTCAGATACAAAGTCATGAAGAACTGGGGAGTGATCGGTGGAATTGCTGCTGCTCTTGCAGC AGGAATATATGTTATCTGGGGTCCCATTACAGAGAGGAAGAAGCGGAGAAAAGGGCTTGTGCCTGGCCTT GTCAACTTGGGGAACACCTGCTTCATGAACTCCCTGCTGCAGGGTTTGTCTGCCTGCCCCGCGTTTGTCA AGTGGCTGGAGGAGTTTACCACCCAGTACTCCAGGGACCAGCAGGGGCCGCACACACACCAGTGCCTGTC CTTGACACTGCTGAGCCTCCTGAAAGCTCTGTCCTGCCAAGAAGTCACTGAGGAAGAGGTCTTGGACGCC AGCTGCCTGCTGGACGTCCTGAGAATGTACCGGTGGCAGATCTCGTCCTTTGAGGAGCAGGATGCTCACG AACTGTTCCATGTCATCACCTCATCCCTGGAGGACGAGCGGGACCGCCAGCCTCGGGTCACCCACTTGTT TGATGTGCACTCCCTGGAGCAGCAGTCAGAAATGGCTCCCAGACAAGTCACCTGCCACACGAGAGGGTCC CCTCACCCCACGACCAATCCCTGGAAGTCTCAGCACCCCTTCCATGGGAGGCTCAGCAGTAACATGGTGT GCAAGCACTGTGAGCACCAGAGCCCTGTTCGGTTTGACACCTTTGATAGCCTTTCCCTCAGTATTCCAGC TGCTACTTGGGGGCACCCACTGACACTGGACCACTGCCTTCACCACTTCATCTCATCCGAGTCGGTGCGA GACGTTGTGTGTGACAACTGTACAAAGATTGAAGCAAAAGGGACCCAGAACGGGGAAAAGGTGGAGCACC AGAGGACCACTTTCGTTAAACAGTTAAAGCTAGGAAAGCTGCCACAGTGCCTCTGCATCCACCTGCAGCG GCTCAGCTGGTCCAGCCAAGGCACGCCCTTGAAGCGGCATGAGCACGTGCAATTTAACGAGTTCCTGATG ATGGACTTCTACAAGTACCGCCTCCTGGGACATAAACCCAGCCAGCATGGCCCCAAAGCCACTGAGAGCC CAGGGTCTGCCCTGGGAGTACAGGACACACAGGCGGCCCCGAAGCCAGGTCTGAGTCAGCCAGCGGCCCC CAAGACACAGTTTTTTATGAATGGCGCCTGCTCCCCGTCTTTGTTGCCAGCCCTGCCTTCTCCGATGGCT TTCCCTCTACCAGTGGCTCCTGACTACAGCTCCTCCATGTACCTCTTCCGCTTGATGGCAGTTGTCGTCC ACCATGGAGACATGCACTCTGGACACTTTGTCACCTACAGACGGTCACCACCTTCTGCCAAGAACCCTCT CTCAACCAGCAACCAGTGGCTGTGGATTTCCGATGACACTGTCCGAAAGGCCAGCCTGCAGGAGGTCCTG TCCTCTAGTGCCTACCTGCTGTTCTATGAGCGAGTTCTGTCCAGGGTGCAGCAGCAGGGGCGAGAGTACA GGTCAGAGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR213092 representing NM_001107153
Red=Cloning site Green=Tags(s) MLSSRAQAARTAADKALQRFLRTGAAVRYKVMKNWGVIGGIAAALAAGIYVIWGPITERKKRRKGLVPGL VNLGNTCFMNSLLQGLSACPAFVKWLEEFTTQYSRDQQGPHTHQCLSLTLLSLLKALSCQEVTEEEVLDA SCLLDVLRMYRWQISSFEEQDAHELFHVITSSLEDERDRQPRVTHLFDVHSLEQQSEMAPRQVTCHTRGS PHPTTNPWKSQHPFHGRLSSNMVCKHCEHQSPVRFDTFDSLSLSIPAATWGHPLTLDHCLHHFISSESVR DVVCDNCTKIEAKGTQNGEKVEHQRTTFVKQLKLGKLPQCLCIHLQRLSWSSQGTPLKRHEHVQFNEFLM MDFYKYRLLGHKPSQHGPKATESPGSALGVQDTQAAPKPGLSQPAAPKTQFFMNGACSPSLLPALPSPMA FPLPVAPDYSSSMYLFRLMAVVVHHGDMHSGHFVTYRRSPPSAKNPLSTSNQWLWISDDTVRKASLQEVL SSSAYLLFYERVLSRVQQQGREYRSEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001107153 |
| ORF Size | 1551 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001107153.1, NP_001100623.1 |
| RefSeq Size | 3362 bp |
| RefSeq ORF | 1554 bp |
| Locus ID | 304579 |
| UniProt ID | D3ZPG5 |
| MW | 58.2 kDa |
| Gene Summary | Deubiquitinating enzyme tethered to the mitochondrial outer membrane that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PRKN): hydrolyzes ubiquitin attached by parkin on target proteins, such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy (PubMed:24896179). Preferentially cleaves 'Lys-6'- and 'Lys-11'-linked polyubiquitin chains, 2 types of linkage that participate to mitophagic signaling. Does not cleave efficiently polyubiquitin phosphorylated at 'Ser-65' (By similarity). Acts as negative regulator of mitochondrial fusion by mediating deubiquitination of MFN1 and MFN2 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN213092 | Usp30 (untagged ORF) - Rat ubiquitin specific peptidase 30 (Usp30), (10 ug) |
CNY 6,650.00 |
|
| RR213092L3 | Lenti ORF clone of Usp30 (Myc-DDK-tagged ORF) - Rat ubiquitin specific peptidase 30 (Usp30), (10 ug) |
CNY 8,550.00 |
|
| RR213092L4 | Lenti ORF clone of Usp30 (mGFP-tagged ORF) - Rat ubiquitin specific peptidase 30 (Usp30), (10 ug) |
CNY 9,310.00 |
