Pask (NM_001009362) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MGC93882
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR213709 representing NM_001009362 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGACAGAGGTCCACCAGTTCTTGCTGAGGACTGGAAGTCTGAGAGTCTTCCTGCCCAAGAGGGCC CAGCTACCCAGGCTTTCGTTGAGCCCAGCAAGCCTCTCTCCATAGCCCATAGACACCTGAACAGGAAGAA TGGGCTTTCTAGACTCTGCCAGAGCCGAATGGCACTCTCCGAGGATAGGTGGAGCTCTTACTGTCTTTCA TCATTGGCTGCCCAAAACATCTGCACAAGCAAACTGCACTGCGCTGCTACACCTGACTATGCTGACCCTA TAGCTGGCCCACTGGGCAGTACATCGTGCTGCTCCTTGCTACGTGGCCTAGCTTCAGGGTGCTCTGGACC TCTGCTCTCGACCCCTGTGTGCAACCCTAACAAGGCTGTCTTTACTGTGGATGCCAAGACCACAGAGATT CTGGTTGCCAATGACAAAGCCTGCAGCCTCCTGGGGTATAGCAGTCATGACCTGATTGGCCAAAAGCTTA CACAGTTCTTCCTCAAGTCTGATTCCGAGGTTGTGGAAGCCCTCAGCGAGGAGCATGTGGAGGCTGATGG CCACGCTGCAGTAGTGTTCGGCACGGTGGTGGACATTGTCAGCCGAAGTGGGGAGAAGATTCCCGTCTCT GTGTGGATAAAGAGGTTGCAACAAGATCGTGGCTTGTGCTGTGTAGTGGTTCTGGAGCCTGTGGAGAGGG TCTCAGCCTGGGTCGCCTTCCAGAGTGATGGAACTATCACGTCATGCGACAGCCTCTTTGCTCATCTTCA TGGGTTCACATCTCCAAAGGATGTGGTTGGGCAGTGTGTCATAGACTTGATTCCATCTATACAGCTCCCT CCTCCTGGCCAACATGTCCCAAAGAGTCTCAAGATTCAAAGGTCTGTTGGGAGAGCCAGAGACGGGACCA CCTTCCCTCTCAGTCTGAAGTTGAAATCTAAGACCAGTGGAAAGGCTGTAGCTGAAAGTGAGGTGGCCTC AGAACCAGGTTACCAGGCTTCTGTGTGGGTGTTCTGCACCATCAGTGGCCTCATCACTCTCCTGCCTGAT GGCACAATTTATGGCATCAACCACAGCTTTGCACTAATGTTGTTTGGTTATGGAAAAACAGAGCTCCTGG GCAAGAACATCACTTGCCTGATTCCTGGTTTCTACCACTGCATGGACCTTGCATATGATAGTTCTGTCCA GCTGCCAGATCTGGTCAACTGCCTGGACATTGGCAGCAAGAGTGGGCCTGGGGAGATGAGTTCTGATGCC AAGCAGGACTGGGAGCTGGCCAGTGGGGCCCAAGATCCAAGAATTGATGTGTTGACACGGGGCCACATGC CATCACAAGATGAGACCCTGAAGCTGCGGGGAGGACAAGTCTCGCCTAGGACTCAGACTCAGCTGGAAAC TGGACGCCACATACTTCCTTCTTGTGCATCTCAGCCCTCCCTGGGGGTAGACAGCAGCCCAGTGGACGGT GAGCGGTCATTACCCACAGACCAGCGGAACGTTCCAAAGAGAAACCTGCTAGCCCATGCTGAACAGTTAC TATCTCAGACCCAACCAGACACCCTGGAAATATCCCCACCAGTCCTTAAAGAACTGTCTGAAATTCAGAA AAATATCTCAGAAGAAAGCCCACTAACCCACAGGAAATGGTTATTGTCCAAGGCCCAGAAAGACACCACA AAAGGAAGCCTGCCAATCCATGATGAGAAGTTACTGCTTGCAAGCCAGCATATCCATGTCTTGGGAAAGG AAAATCCTTCAGCAGCAGAGAGCTACAGGGAGGGTCTTTTAGCAGAAAGCAGGTCCAAACCAGTGGATGC AAAACCGTTTGCTTCATGTGAAGATTCTGAGCTTCTAGTCCCAGCTAAGGACAGAAGCAACAGAGCGGAC ATGAGTAGTCTGCAACAGGAAGCTCAATTGGAGCTGATGGGTATCAGTAGTCCCGGTAGTCCTAACCCCT GGGCTGATGCCACCATGCCTGAGCCCCACACCACAGGGCAGATACCAGGAGGGAGCCTGACATACTGCCC ACAGCATAGAAGCGAGTGGATCTTACAACAGCGAGGCCAGGACTCGGCTCCCAGCCCCTCTGCAATGGGG TGTGTCTTTGTTGGGACACCCACTCTGGATGAACCATGGCCAGGAGTGAGAAATGATCGAGAAGAGCTGC AGACTTGCTTGATTAAGGAACAGTTGTCCAAGTCAAGCTTTGGGGGAGCTCTAGGAATCTCCTGTGTGGA ACTTGTCCCAGCAGAACATCCTCCTTTCACTGCCCCCGTGTCCTTCTGTGATTGGGGAGGCCGAGACCTA CATGCTAGCCGCTCAGGCAGCTCCTCAGCCTGCTATGCTTTGGCCACTGACCTCCCTGGTGTCTTGGAGG CAGTGGAAGCCCAGGAGGCTGATGTGAGTTCTTATTCCTGGAACCTCAGGGAGCTCTTTCTCAATGACCG GACAGACAGAACTCCATCACATTGTTCCTGTACCACTTCTGAACTTAGCGAGACACCATCTCTTTCAGGG GTGGGGTCAGATTTAGATGTGGGTATTCTGCACAGGCAGACTTCAGATATTTTAGCTCACCGAGAGATGT TGCTTCTTAGTGGGACCTATTTTGACCTTAGGGAAGGCCATCGGTTCCAGGAGATGGGAGCAGGACATGA TGGAGCAGAACTGTCTAACATTTCTTTGGTATCCTCTGAACACTATGAAACTAGTGACATAGAAAGCCTA GGTTGTGACCCTCCCATACCAGATCCTGGTCCTAACAATATGTGCCTATCAGCAGAGAAGCCTAGACTGA GTGCCCAGATCACTTCCACTCCTGTGGCACCTGGGCCCACCAGCCTGCAGCAGGAGATCCAGGAGGGCAT CTTCTCTGGAAGCTGCTACCACCGAGATGGCTTACGACTGAGTGTACAGTTTGAAGTAAAGCGTGTGCAG CTCCAGGGCTCTGCAACCCTGTTCTGCTGCTGGCTGGTGAAGGACTTCTTACACAGTCACCGGGACTCAG CCACCCGAACCCGCCTGTTCCTTGCCAGCCTGCCAAGCTCTACCCACTCCATGCCTGACCTCTCTGGATC CAGCTTTGGAGAGATGCTCAGAGCCAAGCCTTGGTTTGAGGAGTCCCCAACACCCGCAGAGCTGGAGGGG CTGGCAGCCTGTGAGGGCGAGTACTCCCATAAGTACAACACCCTGCGCCCACTCGGCAGTGGCGCCTTTG GCTTTGTGTGGACTGCAGTGGAGAAGGAAGGCAACAAAGAGGTGGTGGTGAAGTTTATTAAGAAGGAGAA GGTTTTAGAGGATTGTTGGGTTGAAGATCCCAAACTTGGAAGAGTTCCTTTAGAGATTTCAATTCTGTCC AAGGTGGAACATGCCAACATAATCAAGATTGTGGATGTATTCGAAAACCAAGGGTTCTTTCAGCTAGTGA TGGAAAAGCATGGCTCTGGCTTGGACCTCTTTGCTTTCATTGACCGTCATCCCTGCTTGGATGAGCCCCT GGCGAGTTACATCTTCCGACAACTGGTGTCTGCTGTAAGATACTTGCGTTCCCAAGGCATCATCCATAGA GACATCAAGGATGAGAACATTGTGATTGCTGAGGACTTCACAATTAAGCTGATAGATTTTGGCTCAGCTG CCTACTTAGAGAAGGGCAAACTATTTTATACCTTTTGTGGAACAATTGAATACTGTGCACCTGAAGTTCT CATTGGAAATCCCTACAGAGGGCCAGAGCTGGAGATGTGGTCTCTCGGGGTCACGCTGTACACGCTCATC TTCGAGGAGAATCCCTTCTGTGAGGTGGAGGAGACCATGGAGGCAGTTATTCATCCCCCCTTCCTGGTTT CCCAAGAACTTATGAGTCTTCTGTCTGGGCTGCTGCAGCCTTGCCCTGAGCAGCGGACCACTTTGGAGAA GCTGATTGTGGACCCCTGGGTGACACAGCCTGTGAACCTTGATAACTACACCTGGGAAGAGGTGTGTCGG ACCAGCCAGCCAGAAAGTGGCCTGATGTCAGCTGCAAGTCTGGAGCTTGCGAGTAGGAGTCCAAGTGAAG TGGCTCAGACAGAGGGTCTTTGTAGACCTCCTGCTCCCAAGGAGGCTCCTGGTAACCACTGCTTGCATCT TGAGGATCCCTCGTCACCAGTCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR213709 representing NM_001009362 Red=Cloning site Green=Tags(s) MEDRGPPVLAEDWKSESLPAQEGPATQAFVEPSKPLSIAHRHLNRKNGLSRLCQSRMALSEDRWSSYCLS SLAAQNICTSKLHCAATPDYADPIAGPLGSTSCCSLLRGLASGCSGPLLSTPVCNPNKAVFTVDAKTTEI LVANDKACSLLGYSSHDLIGQKLTQFFLKSDSEVVEALSEEHVEADGHAAVVFGTVVDIVSRSGEKIPVS VWIKRLQQDRGLCCVVVLEPVERVSAWVAFQSDGTITSCDSLFAHLHGFTSPKDVVGQCVIDLIPSIQLP PPGQHVPKSLKIQRSVGRARDGTTFPLSLKLKSKTSGKAVAESEVASEPGYQASVWVFCTISGLITLLPD GTIYGINHSFALMLFGYGKTELLGKNITCLIPGFYHCMDLAYDSSVQLPDLVNCLDIGSKSGPGEMSSDA KQDWELASGAQDPRIDVLTRGHMPSQDETLKLRGGQVSPRTQTQLETGRHILPSCASQPSLGVDSSPVDG ERSLPTDQRNVPKRNLLAHAEQLLSQTQPDTLEISPPVLKELSEIQKNISEESPLTHRKWLLSKAQKDTT KGSLPIHDEKLLLASQHIHVLGKENPSAAESYREGLLAESRSKPVDAKPFASCEDSELLVPAKDRSNRAD MSSLQQEAQLELMGISSPGSPNPWADATMPEPHTTGQIPGGSLTYCPQHRSEWILQQRGQDSAPSPSAMG CVFVGTPTLDEPWPGVRNDREELQTCLIKEQLSKSSFGGALGISCVELVPAEHPPFTAPVSFCDWGGRDL HASRSGSSSACYALATDLPGVLEAVEAQEADVSSYSWNLRELFLNDRTDRTPSHCSCTTSELSETPSLSG VGSDLDVGILHRQTSDILAHREMLLLSGTYFDLREGHRFQEMGAGHDGAELSNISLVSSEHYETSDIESL GCDPPIPDPGPNNMCLSAEKPRLSAQITSTPVAPGPTSLQQEIQEGIFSGSCYHRDGLRLSVQFEVKRVQ LQGSATLFCCWLVKDFLHSHRDSATRTRLFLASLPSSTHSMPDLSGSSFGEMLRAKPWFEESPTPAELEG LAACEGEYSHKYNTLRPLGSGAFGFVWTAVEKEGNKEVVVKFIKKEKVLEDCWVEDPKLGRVPLEISILS KVEHANIIKIVDVFENQGFFQLVMEKHGSGLDLFAFIDRHPCLDEPLASYIFRQLVSAVRYLRSQGIIHR DIKDENIVIAEDFTIKLIDFGSAAYLEKGKLFYTFCGTIEYCAPEVLIGNPYRGPELEMWSLGVTLYTLI FEENPFCEVEETMEAVIHPPFLVSQELMSLLSGLLQPCPEQRTTLEKLIVDPWVTQPVNLDNYTWEEVCR TSQPESGLMSAASLELASRSPSEVAQTEGLCRPPAPKEAPGNHCLHLEDPSSPVS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001009362
ORF Size	4155 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001009362.1, NP_001009362.1
RefSeq Size	4562 bp
RefSeq ORF	4158 bp
Locus ID	301617
MW	151.2 kDa

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