Map1b (NM_019217) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Mtap1b
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR214092 representing NM_019217 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACCGTGGTGGTGGAAGCCACCGAGCCGGAGCCATCGGGCAGCATCGGCAACCCGGCGGCGACCA CCTCGCCCAGCCTGTCGCACCGCTTCCTAGACAGCAAGTTCTACTTGCTGGTGGTGGTCGGCGAGACGGT GACCGAAGAGCACCTGAGGCGTGCCATCGGCAACATCGAGCTGGGGATCCGATCGTGGGACACAAACCTG ATCGAGTGCAACTTGGACCAAGAGCTCAAACTTTTCGTGTCTCGACACTCCGCGAGATTCTCTCCTGAAG TTCCAGGACAAAAGATCCTCCATCACCGAAGTGACGTCTTAGAAACTGTAGTTCTGATCAACCCTTCGGA TGAAGCAGTCAGCACCGAGGTGCGTTTGATGATCACTGACGCCGCCCGCCATAAACTGCTGGTGCTCACC GGACAGTGCTTTGAGAACACTGGAGAGCTCATCCTCCAGTCAGGCTCTTTCTCCTTCCAGAACTTCATAG AGATTTTCACCGACCAAGAGATTGGGGAGCTCCTAAGCACCACCCATCCTGCCAACAAAGCCAGCCTCAC CCTCTTCTGCCCTGAGGAAGGAGACTGGAAGAACTCCAACCTTGACAGACACAATCTCCAAGACTTCATC AACATCAAGCTCAACTCAGCTTCTATCTTGCCAGAAATGGAGGGACTTTCTGAGTTCACCGAGTACCTCT CGGAGTCTGTCGAAGTCCCCTCCCCCTTTGACATCCTGGAGCCCCCGACCTCGGGCGGATTTCTGAAGCT CTCCAAGCCTTGTTGTTACATTTTTCCGGGGGGCCGCGGGGACTCTGCCCTGTTCGCAGTGAACGGATTC AACATGCTCATTAACGGAGGATCAGAAAGAAAGTCCTGCTTCTGGAAGCTCATTCGGCACTTGGACCGGG TGGACTCCATCCTGCTCACCCACATTGGGGATGACAACTTGCCCGGGATCAACAGCATGTTGCAACGCAA GATTGCAGAGCTGGAAGAGGAGCGGTCCCAGGGCTCCACCAGCAACAGTGACTGGATGAAAAACCTCATC TCCCCTGACTTGGGGGTTGTGTTTCTCAATGTACCTGAAAATCTGAAAAACCCAGAACCCAACATCAAGA TGAAGAGAAGTACAGAAGAAGCATGCTTCACCCTCCAGTACCTAAACAAACTGTCCATGAAACCAGAGCC TTTATTTAGAAGTGTAGGCAATGCCATTGAGCCTGTCATCCTGTTCCAAAAAATGGGAGTGGGTAAACTG GAGATGTACGTGCTTAACCCAGTCAAAAGCAGCAAGGAAATGCAGTATTTCATGCAGCAGTGGACTGGAA CCAACAAAGACAAGGCTGAACTTATCCTGCCCAATGGTCAAGAAGTAGACATCCCGATTTCCTACCTGAC TTCCGTCTCGTCTTTGATTGTGTGGCACCCAGCCAACCCTGCTGAGAAAATCATCCGGGTTCTGTTTCCT GGAAACAGCACCCAGTACAACATCCTAGAAGGGCTGGAAAAACTCAAACATCTAGACTTCCTAAAGCAGC CACTGGCCACCCAAAAAGATCTCACTGGCCAGGTGTCCACCCCCCCAGTGAAACAGGTCAAGTTGAAACA GCGGGCTGACAGCCGAGAGAGTCTGAAGCCAGCCACAAAACCACTTTCCAGTAAATCAGTGAGGAAGGAG TCCAAAGAGGAGGCCCCTGAAGCCACAAAAGCCAGCCAAGTGGAAAAAACACCCAAAGTTGAAAGCAAAG AGAAAGTGATAGTGAAAAAAGACAAGCCAGGAAAGGTAGAAAGTAAGCCATCGGTGACGGAAAAGGAGGT GCCCAGCAAAGAGGAGCAGTCGCCCGTCAAAGCTGAGGTGGCTGAGAAGGCGGCCACGGAGAGCAAACCC AAAGTCACCAAAGACAAAGTGGTAAAAAAGGAAATAAAGACAAAACCCGAAGAAAAGAAAGAGGAGAAGC CCAAGAAGGAAGTGGCTAAAAAGGAAGACAAAACTCCCCTCAAGAAAGACGAGAAGCCCAAAAAGGAAGA GGCGAAGAAGGAGATCAAGAAAGAAATCAAAAAGGAAGAGAAAAAGGAGCTGAAGAAAGAGGTGAAGAAG GAAACGCCCCTGAAGGACGCCAAGAAGGAGGTGAAGAAAGACGAGAAGAAAGAAGTTAAAAAGGAAGAGA AGGAACCCAAAAAGGAGATTAAGAAGATCTCCAAGGACATAAAGAAATCCACTCCTCTGTCAGACACAAA GAAACCGGCTGCATTGAAACCAAAAGTAGCAAAGAAAGAAGAGCCCACCAAGAAGGAGCCTATTGCTGCT GGGAAACTCAAGGACAAGGGGAAGGTCAAAGTCATTAAAAAGGAAGGCAAGACCACAGAGGCCGCTGCCA CAGCTGTTGGCACTGCTGCCGTGGCTGCAGCAGCCGGAGTAGCGGCCAGCGGTCCTGCCAAAGAACTTGA AGCTGAGCGGTCCCTCATGTCGTCCCCTGAGGATCTAACCAAGGACTTTGAGGAGCTAAAGGCTGAGGAG ATCGATGTAGCGAAGGACATCAAGCCTCAGCTGGAGCTCATTGAAGATGAAGAGAAACTGAAGGAAACCG AGCCGGGAGAAGCCTACGTCATTCAGAAAGAGACGGAAGTCAGCAAAGGTTCTGCTGAGTCACCTGATGA AGGGATCACCACCACTGAGGGGGAAGGGGAGTGCGAGCAAACCCCCGAGGAGCTGGAGCCAGTTGAGAAG CAGGGCGTGGATGACATCGAGAAGTTCGAGGATGAAGGCGCTGGTTTTGAAGAATCCTCAGAGGCCGGAG ACTACGAAGAGAAGGCAGAAACTGAGGAGGCCGAGGAGCCGGAAGAAGACGGGGAAGACAATGTGAGCGG GAGCGCCTCGAAGCACAGCCCCACAGAAGACGAAGAAATCGCTAAGGCTGAGGCGGACGTACACATCAAG GAGAAGAGGGAGTCTGTGGCCAGCGGCGATGACCGGGCCGAAGAAGACATGGATGAAGCGCTTGAGAAAG GAGAAGCTGAACAGTCTGAGGAGGAGGGTGAGGAGGAGGAGGACAAAGCAGAGGACGCCAGAGAGGAAGA CCATGAGCCCGACAAAACTGAGGCTGAAGATTATGTGATGGCTGTGGTTGACAAGGCCGCGGAGGCCGGA GTCACCGAGGATCAGTATGATTTCCTGGGGACACCGGCCAAGCAACCTGGAGTCCAGTCTCCTAGCCGAG AACCCGCGTCTTCAATTCATGATGAGACCCTACCCGGAGGCTCCGAGAGCGAGGCCACTGCTTCAGATGA GGAGAATCGAGAAGACCAGCCTGAGGAATTCACTGCTACCTCCGGATATACTCAGTCCACCATCGAGATA TCTAGTGAGCCGACTCCAATGGATGAGATGTCCACTCCTCGAGATGTGATGACCGACGAGACCAACAATG AGGAGACAGAGTCCCCGTCTCAGGAGTTCGTGAACATTACCAAATACGAGTCTTCGCTGTACTCTCAGGA GTACTCCAAACCTGTGGTTGCATCATTCAATGGATTGTCAGACGGGTCAAAGACAGACGCCACTGACGGT AGGGATTACAACGCTTCCGCCTCCACCATATCACCACCTTCGTCCATGGAAGAAGACAAATTCAGCAAGT CTGCTCTTCGTGACGCTTACCGCCCAGAAGAGACGGACGTGAAAACCGGTGCCGAGTTGGACATCAAAGA TGTTTCGGATGAGAGACTTAGCCCAGCCAAGAGTCCATCCCTGAGTCCTTCTCCACCATCACCCATAGAG AAGACTCCCCTGGGTGAACGTAGCGTGAATTTCTCTCTGACACCCAACGAGATCAAAGCCTCTGCAGAGG GAGAGGCAACAGCAGTAGTGTCCCCCGGAGTGACCCAAGCAGTAGTTGAAGAACACTGTGCCAGTCCTGA GGAGAAGACCTTGGAGGTAGTGTCACCGTCTCAGTCTGTGACAGGCAGTGCGGGCCACACACCTTACTAC CAATCTCCCACCGACGAAAAGTCCAGTCACCTACCTACAGAAGTCACTGAGAACGCGCAGGCAGTCCCGG TGAGCTTTGAATTCACTGAGGCCAAAGATGAGAACGAGAGGTCGTCCATCAGCCCCATGGATGAACCTGT GCCTGACTCAGAGTCTCCTATCGAGAAAGTTCTGTCTCCGTTACGCAGCCCTCCCCTTATTGGATCCGAG TCCGCATATGAAGACTTCCTGAGTGCGGATGACAAGGCTCTTGGCAGACGTTCAGAAAGCCCCTTTGAAG GGAAGAATGGAAAGCAAGGCTTCTCAGACAAAGAAAGCCCAGTTTCTGACCTGACTTCCGATCTTTACCA AGACAAGCAGGAAGAGAAAAGCGCGGGCTTCATACCGATAAAGGAAGACTTTAGTCCAGAAAAGAAAGCC AGCGATGCTGAAATCATGAGTTCTCAATCAGCTCTGGCTTTGGATGAAAGGAAACTGGGAGGAGATGGAT CTCCAACGCAAGTAGATGTCAGTCAGTTTGGCTCTTTCAAAGAAGACACCAAGATGTCCATTTCGGAAGG CACCGTTTCAGACAAGTCCGCCACGCCTGTGGATGAGGGCGTGGCCGAAGACACCTATTCACACATGGAA GGTGTGGCCTCAGTGTCAACCGCCTCTGTGGCTACCAGCTCGTTTCCAGAGCCAACCACAGATGACGTGT CTCCTTCTCTCCACGCTGAAGTGGGCTCTCCACATTCCACAGAGGTGGATGACTCCCTGTCGGTGTCGGT GGTGCAAACACCAACTACTTTCCAGGAAACAGAAATGTCTCCGTCTAAAGAAGAGTGCCCAAGACCAATG TCGATTTCTCCTCCTGACTTCTCCCCTAAGACAGCCAAATCCAGGACACCAGTTCAAGATCACCGATCCG AACAGTCTTCAATGTCTATTGAATTCGGTCAGGAATCCCCCGAGCATTCTCTTGCTATGGACTTTAGTCG GCAGTCTCCAGACCACCCTACTGTGGGTGCTGGTATGCTTCACATCACCGAAAATGGGCCAACTGAGGTG GACTACAGTCCCTCCGATATCCAGGACTCTAGTTTGTCACATAAGATTCCGCCGACAGAAGAGCCATCCT ACACCCAGGATAATGATCTGTCCGAGCTCATCTCTGTGTCTCAGGTGGAGGCTTCCCCATCCACCTCTTC TGCTCACACTCCTTCTCAGATAGCCTCTCCTCTTCAGGAAGACACTCTCTCTGATGTCGTTCCTCCCAGA GATATGTCCTTATATGCCTCGCTTGCGTCTGAGAAAGTGCAGAGCCTGGAAGGAGAGAAACTCTCTCCAA AATCCGATATTTCTCCGCTCACCCCTCGAGAGTCCTCACCTACATATTCACCTGGCTTTTCAGATTCTAC CTCTGGAGCTAAAGAGAGTACAGCGGCTTACCAAACCTCCTCTTCCCCACCAATAGATGCAGCAGCCGCA GAGCCCTACGGCTTCCGCTCCTCAATGTTATTTGATACAATGCAGCATCACCTGGCCTTGAGTAGAGATT TGACCACATCTAGTGTGGAGAAGGACAATGGAGGGAAGACACCCGGTGACTTTAACTATGCCTATCAAAA GCCCGAGAGCACCACCGAATCCCCAGATGAAGAAGATTATGACTATGAATCTCACGAGAAAACCATCCAG GCCCACGATGTGGGTGGTTACTACTATGAGAAGACAGAGAGAACCATAAAATCCCCATGTGACAGTGGAT ACTCCTATGAGACCATTGAGAAGACCACCAAGACCCCAGAAGATGGTGGCTACTCCTGTGAAATTACCGA GAAAACCACTCGGACCCCTGAAGAGGGCGGGTACTCGTATGAGATCAGCGAGAAGACAACACGAACCCCT GAAGTAAGTGGCTACACCTATGAGAAGACCGAGAGGTCCAGAAGGCTCCTCGATGACATTAGCAATGGCT ACGATGACACTGAGGATGGTGGCCACACACTTGGCGACTGTAGCTATTCCTACGAAACCACTGAGAAAAT TACCAGCTTTCCTGAATCTGAAAGCTATTCCTATGAGACAACTACAAAAACAACACGGAGTCCAGACACC TCTGCATACTGTTACGAGACCATGGAGAAGATCACCAAGACCCCACAGGCATCCACATACTCCTATGAGA CCTCAGACCGATGCTACACTCCAGAAAGGAAGTCCCCCTCGGAGGCACGCCAGGATGTTGACTTGTGTCT GGTGTCCTCCTGTGAATTCAAGCATCCCAAGACCGAGCTCTCACCTTCCTTCATTAATCCAAACCCTCTC GAGTGGTTTGCTGGGGAAGAGCCCACTGAAGAATCTGAGAAGCCTCTCACTCAGTCTGGAGGAGCCCCCC CACCTTCAGGAGGAAAACAACAGGGCAGACAATGCGATGAAACTCCACCCACCTCAGTCAGTGAGTCAGC TCCATCCCAGACGGACTCTGATGTTCCCCCAGAGACAGAAGAGTGCCCCTCCATCACAGCTGATGCCAAC ATTGACTCTGAAGATGAGTCAGAAACCATCCCCACAGACAAAACGGTTACGTACAAACACATGGACCCGC CTCCAGCCCCCATGCAAGACCGAAGCCCTTCTCCTCGCCACCCTGATGTGTCCATGGTGGATCCAGAGGC CTTGGCTATTGAGCAGAACCTAGGCAAGGCTCTGAAAAAGGATCTGAAGGAGAAGGCCAAGACCAAGAAA CCAGGCACAAAGACCAAGTCCTCTTCACCTGTCAAAAAGGGTGATGGGAAGTCCAAGCCTTCAGCAGCTT CCCCCAAACCAGGAGCCTTGAAGGAATCCTCTGACAAGGTGTCCAGAGTGGCTTCTCCCAAGAAGAAAGA GTCTGTGGAGAAAGCTATGAAGACCACCACCACTCCTGAGGTCAAAGCCACACGAGGGGAAGAGAAGGAC AAGGAAACTAAGAATGCAGCCAATGCTTCTGCATCCAAGTCAGTGAAGACTGCAACAGCAGGACCAGGAA CCACTAAGACGGCCAAGTCGTCCACCGTGCCTCCCGGCCTCCCTGTGTATTTGGACCTCTGCTATATTCC CAACCACAGCAACAGTAAGAATGTCGATGTTGAGTTTTTCAAGAGAGTGAGGTCATCTTACTACGTGGTG AGTGGGAACGACCCTGCCGCGGAGGAGCCCAGCCGGGCTGTCCTGGATGCCTTGTTGGAAGGGAAGGCTC AGTGGGGAAGCAACATGCAGGTGACTCTGATCCCAACACATGACTCTGAGGTGATGAGGGAGTGGTACCA GGAGACCCACGAGAAGCAGCAAGACCTCAACATCATGGTCCTAGCAAGCAGTAGTACAGTGGTCATGCAA GACGAGTCCTTCCCTGCATGCAAGATAGAACTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR214092 representing NM_019217 Red=Cloning site Green=Tags(s) MATVVVEATEPEPSGSIGNPAATTSPSLSHRFLDSKFYLLVVVGETVTEEHLRRAIGNIELGIRSWDTNL IECNLDQELKLFVSRHSARFSPEVPGQKILHHRSDVLETVVLINPSDEAVSTEVRLMITDAARHKLLVLT GQCFENTGELILQSGSFSFQNFIEIFTDQEIGELLSTTHPANKASLTLFCPEEGDWKNSNLDRHNLQDFI NIKLNSASILPEMEGLSEFTEYLSESVEVPSPFDILEPPTSGGFLKLSKPCCYIFPGGRGDSALFAVNGF NMLINGGSERKSCFWKLIRHLDRVDSILLTHIGDDNLPGINSMLQRKIAELEEERSQGSTSNSDWMKNLI SPDLGVVFLNVPENLKNPEPNIKMKRSTEEACFTLQYLNKLSMKPEPLFRSVGNAIEPVILFQKMGVGKL EMYVLNPVKSSKEMQYFMQQWTGTNKDKAELILPNGQEVDIPISYLTSVSSLIVWHPANPAEKIIRVLFP GNSTQYNILEGLEKLKHLDFLKQPLATQKDLTGQVSTPPVKQVKLKQRADSRESLKPATKPLSSKSVRKE SKEEAPEATKASQVEKTPKVESKEKVIVKKDKPGKVESKPSVTEKEVPSKEEQSPVKAEVAEKAATESKP KVTKDKVVKKEIKTKPEEKKEEKPKKEVAKKEDKTPLKKDEKPKKEEAKKEIKKEIKKEEKKELKKEVKK ETPLKDAKKEVKKDEKKEVKKEEKEPKKEIKKISKDIKKSTPLSDTKKPAALKPKVAKKEEPTKKEPIAA GKLKDKGKVKVIKKEGKTTEAAATAVGTAAVAAAAGVAASGPAKELEAERSLMSSPEDLTKDFEELKAEE IDVAKDIKPQLELIEDEEKLKETEPGEAYVIQKETEVSKGSAESPDEGITTTEGEGECEQTPEELEPVEK QGVDDIEKFEDEGAGFEESSEAGDYEEKAETEEAEEPEEDGEDNVSGSASKHSPTEDEEIAKAEADVHIK EKRESVASGDDRAEEDMDEALEKGEAEQSEEEGEEEEDKAEDAREEDHEPDKTEAEDYVMAVVDKAAEAG VTEDQYDFLGTPAKQPGVQSPSREPASSIHDETLPGGSESEATASDEENREDQPEEFTATSGYTQSTIEI SSEPTPMDEMSTPRDVMTDETNNEETESPSQEFVNITKYESSLYSQEYSKPVVASFNGLSDGSKTDATDG RDYNASASTISPPSSMEEDKFSKSALRDAYRPEETDVKTGAELDIKDVSDERLSPAKSPSLSPSPPSPIE KTPLGERSVNFSLTPNEIKASAEGEATAVVSPGVTQAVVEEHCASPEEKTLEVVSPSQSVTGSAGHTPYY QSPTDEKSSHLPTEVTENAQAVPVSFEFTEAKDENERSSISPMDEPVPDSESPIEKVLSPLRSPPLIGSE SAYEDFLSADDKALGRRSESPFEGKNGKQGFSDKESPVSDLTSDLYQDKQEEKSAGFIPIKEDFSPEKKA SDAEIMSSQSALALDERKLGGDGSPTQVDVSQFGSFKEDTKMSISEGTVSDKSATPVDEGVAEDTYSHME GVASVSTASVATSSFPEPTTDDVSPSLHAEVGSPHSTEVDDSLSVSVVQTPTTFQETEMSPSKEECPRPM SISPPDFSPKTAKSRTPVQDHRSEQSSMSIEFGQESPEHSLAMDFSRQSPDHPTVGAGMLHITENGPTEV DYSPSDIQDSSLSHKIPPTEEPSYTQDNDLSELISVSQVEASPSTSSAHTPSQIASPLQEDTLSDVVPPR DMSLYASLASEKVQSLEGEKLSPKSDISPLTPRESSPTYSPGFSDSTSGAKESTAAYQTSSSPPIDAAAA EPYGFRSSMLFDTMQHHLALSRDLTTSSVEKDNGGKTPGDFNYAYQKPESTTESPDEEDYDYESHEKTIQ AHDVGGYYYEKTERTIKSPCDSGYSYETIEKTTKTPEDGGYSCEITEKTTRTPEEGGYSYEISEKTTRTP EVSGYTYEKTERSRRLLDDISNGYDDTEDGGHTLGDCSYSYETTEKITSFPESESYSYETTTKTTRSPDT SAYCYETMEKITKTPQASTYSYETSDRCYTPERKSPSEARQDVDLCLVSSCEFKHPKTELSPSFINPNPL EWFAGEEPTEESEKPLTQSGGAPPPSGGKQQGRQCDETPPTSVSESAPSQTDSDVPPETEECPSITADAN IDSEDESETIPTDKTVTYKHMDPPPAPMQDRSPSPRHPDVSMVDPEALAIEQNLGKALKKDLKEKAKTKK PGTKTKSSSPVKKGDGKSKPSAASPKPGALKESSDKVSRVASPKKKESVEKAMKTTTTPEVKATRGEEKD KETKNAANASASKSVKTATAGPGTTKTAKSSTVPPGLPVYLDLCYIPNHSNSKNVDVEFFKRVRSSYYVV SGNDPAAEEPSRAVLDALLEGKAQWGSNMQVTLIPTHDSEVMREWYQETHEKQQDLNIMVLASSSTVVMQ DESFPACKIEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_019217
ORF Size	7383 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_019217.1, NP_062090.1
RefSeq Size	11884 bp
RefSeq ORF	7386 bp
Locus ID	29456
UniProt ID	P15205
MW	269.7 kDa
Gene Summary	binds microtubules and may function as microtubule cross-linkers [RGD, Feb 2006]

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