Rfx7 (NM_001127490) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Rfxdc2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR214947 representing NM_001127490 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGAGGAACAACAACAGCCGCCACCACAGCAGCTCGATGCCCATCAGCAGCTTCCCCTCAGCGCCC CCAACCCCGGGGTGGCTCTGCCAGCCCTTGTGCCCGGGCTGCCAGGGACAGAGGCCAGCGCTCTGCAACA CAAGATCAAGAACTCCATCTGCAAAACTGTACAATCTAAAGTGGACTGCATTTTGCAAGAAGTTGAGAAG TTTACAGACCTAGAGAAACTCTACCTCTACCTTCAACTGCCTTCTGGTCTCAGCAGTGCAGAGAAAAGTG ATCAGAATGCCATGTCATCTAGCCGGGCACAGCAAATGCATGCCTTTTCCTGGATTCGAAATACCTTAGA GGAGCATCCTGAGACTTCACTCCCCAAACAAGAAGTCTATGATGAATACAAGAGCTATTGTGACAATCTT GGCTACCATCCATTAAGTGCTGCTGACTTTGGAAAAATCATGAAGAACGTCTTTCCAAACATGAAGGCAC GCCGTTTGGGCACTAGAGGCAAATCTAAATATTGCTACAGTGGACTAAGGAAAAAAGCTTTTGTTCATAT GCCAACACTGCCCAACCTTGACTTTCATAAAACTGGAGATGGGTTGGAAGGAGTTGAACCATCTGGGCAG CTTCAAAATATTGATGAGGAAGTTATCTCTTCTGCTTGCCGTCTTGTGTGTGAGTGGGCCCAGAAAGTGT TAAGCCAGCCATTTGACACAGTCTTGGAATTAGCCCACTTCCTTGTAAAGAGTCATTATATAGGCACCAA GTCAATGGCAGCTCTGACTGTGATGGCAGCAGCACCAGCAGGACTTAAAGGAATTTCCCAGCCTTCTGCT TTTATACCTACAGCTGAAAGTAATTCCTTTCAACCTCAAGTAAAGACTTTGTCATCTCCAATTGATGCTA AACAGCAGTTGCAACGGAAAATTCAAAAAAAGCAGCAAGAACAGAAACTACAGTCCCCATTACCAGGAGA ATCCTCAGCAAAAAAAACAGAAGGCACTACAGCCAACGGAGTGGCTAATCTACCCAATGGGAACCCTGCG ATCCTTTCTCCCCAGCCCATTGGGATTGTGGTAGCAGCTGTCCCTAGTCCCATTCCGGTCCAGCGGACGA GGCAGTTAGTAACTTCACCCAGTCCGATGAATTCTTCTGATGGCAAGGTTCTTCCCCTCAACGTGCAGGT AGTCACTCAGCACATGCAGTCTGTGAAACAGACACCAAAGACTCCTCAGAACGTCCCAGCCAGTCCTGGT GGGGACCGTTCTGCCAGGCACCGCTACCCTCAGATCTTACCCAAGCCAGCAAACACCAGTGCGCTCACCA TCCGCTCTCCTACGACTGTGCTGTTTACCAGCAGTCCCATCAAAACTGCTGTGGTACCTGCGTCACACAT GAGTTCTCTAAATGTGGTGAAAATGACAACAATATCCCTCACACCCAGTAACAGTAATGCGCCTCTTAAA CATTCTGCCTCCGTCAGCAATGCTACTGGAACAACAGAGGACTCCAGGAGCATCCCTCAGATCAAGAATG GTTCTGTTGTTTCTCTCCAGTCCCCTGGGTCCAGGGCCAGCAGCACTGGGGGAACCTCTGCTGTGGAAGT CAAAATGGAACCAGAAGGTTCATCAGATGAGCATCCTCTGCAGTGCCAAGAGAACGCTGAGGGGACTAAA GCTCCTCTGGCCACATCTAGTGCCCTTTGGGGACAGAAAAGTAATACAGATGGAACAGTATCAAAACCTT CTAATGAAGGAGTCACAGAAGTGAAAACAACTAAGGTCTGTGACCAGAGGGCCAAGTGTAAAAGTCGCTG TAATGAAACTCTGCCAGGCATCTCAGCAGGCAATAATCAAAGCACTGTTACTCTCTCAGTTGCTACTCAG AACTTACCCTTCACCAGCACCAGCTCACCATCTAATGGTGACTCAGTAAATAAAGACCCTAAATTATGCA CTAAAAGTCCAAGAAAACGACTGTCTGCTACACTGCAAGAGTCTCAGGTGCCTCCTGTAAAGAAACCAAT TGTAGAACAGCTTTCTGCAGTTACTACCGAAGGTCAGAAACCAGGCACTGTTAAAAAGGACCAAAAGGTT CCACATTCAGGGAAAACAGAAAGCTCAACAGCAGGTGCTCAGCCTCCTAACAAGGTGTCACTCAGTGTCA GCTCACACATTGTAGAGGATCAGCCCTTGAACCCTACTCTTGTCGCCAGTGAATCAGCTCTGGAGCAGCA GACAACGCCATCATCGTCTCCGGATGTAAAAGTAAAACTTGAAGGGAGTGTCTTTCTTTTGGACCGTGAG TCAAACTCAGATGGCAGCTTTAATCGTAACGAATGGCAACAAGTTACTAAGGATTCTGACTTCATATCTG CCAGCTGTGAGCAACAGCAAGACATTAGTGTGATGACAATTCCTGAGCATCCTGACATCCATGATTTAGA GAAGTCTGTTTGGGAATTAGAAGGGATGCCACAGGACACATACAGCCAGCAGCTCCATAGCCAGATACCA GAATCTTCTCTGAATGAAATACAAGCACAGTCTTCAGATCAGTTACCGTTGCAGTCGGAACTGAAAGAGT TTGAGTCTTCTGTTTCCCAGACAAATGAAAGCTACTTTCCTTTTGATGATGAACTTACACAAGATAGCAT TGTGGAAGAGCTGGTGCTTATGGAGCAGCAGATGTCAATGAATAACTCCCATTCTTATGGTAACTGCTTG GGAATGACCCTTCAGAGTCAGTCAGTAACTCCAGGAGCTCCAATGTCATCTCATGGTTCCAGCTCTCACT TCTATCATCCAATCCATAGCAATGGCACTCCAATTCACACACCTACACCCACACCCACTCCTACACCAAC TCCGACCCCAACTCCAACCCCAACATCTGAAATGATTGCTGGGTCTCAGAGTCTGTCACGGGAGAGCCCT TGTTCCAGGCTTGCCCAGACAACACCTGTGGATAGTGCTTTAGGAAGTAGCCGACACACACCCATTGGTA CTCCTCATTCTAATTGCAGCAGTAGCGTCCCTCCAAGCCCTGTTGAATGCAGGAATCCATTTGCATTCAC CCCAATAAGCTCCAGTATGGCATATCACGATGCCAGCATTGTCTCAAGCAGTCCTGTAAAACCAATGCAA AGACCCATGGCCACTCACCCTGATAAAACCAAGCTTGAATGGATGAATAATGGGTATGGTGGGGTTGGTA ACTCATCAGTTTCTGGTCACGGCATTCTCCCAAGCTATCAGGAACTAGTAGAAGATCGTTTCAGGAAACC TCATGCTTTTGCTGTGCCCGGACAGTCGTATCAGTCTCAGTCCAGACACCATGACACTCATTTTGGTCGT TTGACTCCCGTCTCTCCTGTGCAGCATCAAGGTGCCACTGTAAACACCAACAAGCAAGAAGGGTTTGCAG TCCCCGCTCCTCTTGATAATAAAGGAACTAATTCATCTGCCAGCAGCAACTTCAGGTGCCGGAGTGTAAG CCCTGCTGTCCATCGCCAACGGAATCTTAGTGGAAGCACCCTCTATCCTGTGTCTAATATCCCACGGTCT AATGTAACCCCCTTTGGAAGTCCAGTGACCCCAGAAGTTCATGTTTTCACAAATGTTCACACAGATGCAT GTGCCAACAACATAGCTCAAAGAAGTCAGTCAGTTCCATTAACAGTCATGATGCAGACAGCCTTCCCTAA TGCTCTGCAGAAGCAAACAAACAGTAAAAAAATCACTAATGTTTTGTTGAGTAAACTCGATTCTGACAAT GATGATGCAGTGAGAGGTTTGGGAATGAACAACGTGCCCTCCAATTACACAGCCCGGATGAATCTCACTC AGATTTTGGAAACTTCCCCTGTTTTTCCTAGTGCCAATTCACAAAATATGATCGATTCCAGCACTTCTGT TTATGAATTCCAAACACCATCTTACCTCACCAAAAGTAATAGCACCGATCAGATCAGTTTTTCTCCTGGA GATAATCAAGCACAATCTGAAATTGGAGAACAGCAGTTAGATTTCAATAGCACTGTTAAAGACCTGTTGA GTGGAGACAACCTGCAAACCAGCCAGCAGCTGGGAGGTCAGGTAGCATCTGATCTCACCAACACTGCATC TGATTTCCCTAGCGACATCAGGTTGTCTTCTGACCTCTCAGGCAGCATCAATGATTTGAACACCTTAGAC CCAAATCTACTGTTTGATCCAGGTCGTCAGCAGGGACAGGATGATGAAGCCACACTGGAAGAATTAAAGA ATGACCCACTGTTTCAACAGATTTGCAGTGAGTCAATGAACTCTATGACTTCATCAGGTTTTGAATGGAT AGAAAGCAAGGACCATCCTACTGTTGAAATGTTGGGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR214947 representing NM_001127490 Red=Cloning site Green=Tags(s) MAEEQQQPPPQQLDAHQQLPLSAPNPGVALPALVPGLPGTEASALQHKIKNSICKTVQSKVDCILQEVEK FTDLEKLYLYLQLPSGLSSAEKSDQNAMSSSRAQQMHAFSWIRNTLEEHPETSLPKQEVYDEYKSYCDNL GYHPLSAADFGKIMKNVFPNMKARRLGTRGKSKYCYSGLRKKAFVHMPTLPNLDFHKTGDGLEGVEPSGQ LQNIDEEVISSACRLVCEWAQKVLSQPFDTVLELAHFLVKSHYIGTKSMAALTVMAAAPAGLKGISQPSA FIPTAESNSFQPQVKTLSSPIDAKQQLQRKIQKKQQEQKLQSPLPGESSAKKTEGTTANGVANLPNGNPA ILSPQPIGIVVAAVPSPIPVQRTRQLVTSPSPMNSSDGKVLPLNVQVVTQHMQSVKQTPKTPQNVPASPG GDRSARHRYPQILPKPANTSALTIRSPTTVLFTSSPIKTAVVPASHMSSLNVVKMTTISLTPSNSNAPLK HSASVSNATGTTEDSRSIPQIKNGSVVSLQSPGSRASSTGGTSAVEVKMEPEGSSDEHPLQCQENAEGTK APLATSSALWGQKSNTDGTVSKPSNEGVTEVKTTKVCDQRAKCKSRCNETLPGISAGNNQSTVTLSVATQ NLPFTSTSSPSNGDSVNKDPKLCTKSPRKRLSATLQESQVPPVKKPIVEQLSAVTTEGQKPGTVKKDQKV PHSGKTESSTAGAQPPNKVSLSVSSHIVEDQPLNPTLVASESALEQQTTPSSSPDVKVKLEGSVFLLDRE SNSDGSFNRNEWQQVTKDSDFISASCEQQQDISVMTIPEHPDIHDLEKSVWELEGMPQDTYSQQLHSQIP ESSLNEIQAQSSDQLPLQSELKEFESSVSQTNESYFPFDDELTQDSIVEELVLMEQQMSMNNSHSYGNCL GMTLQSQSVTPGAPMSSHGSSSHFYHPIHSNGTPIHTPTPTPTPTPTPTPTPTPTSEMIAGSQSLSRESP CSRLAQTTPVDSALGSSRHTPIGTPHSNCSSSVPPSPVECRNPFAFTPISSSMAYHDASIVSSSPVKPMQ RPMATHPDKTKLEWMNNGYGGVGNSSVSGHGILPSYQELVEDRFRKPHAFAVPGQSYQSQSRHHDTHFGR LTPVSPVQHQGATVNTNKQEGFAVPAPLDNKGTNSSASSNFRCRSVSPAVHRQRNLSGSTLYPVSNIPRS NVTPFGSPVTPEVHVFTNVHTDACANNIAQRSQSVPLTVMMQTAFPNALQKQTNSKKITNVLLSKLDSDN DDAVRGLGMNNVPSNYTARMNLTQILETSPVFPSANSQNMIDSSTSVYEFQTPSYLTKSNSTDQISFSPG DNQAQSEIGEQQLDFNSTVKDLLSGDNLQTSQQLGGQVASDLTNTASDFPSDIRLSSDLSGSINDLNTLD PNLLFDPGRQQGQDDEATLEELKNDPLFQQICSESMNSMTSSGFEWIESKDHPTVEMLG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001127490
ORF Size	4377 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001127490.1, NP_001120962.1
RefSeq Size	7998 bp
RefSeq ORF	4380 bp
Locus ID	315804
MW	157.4 kDa

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