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Rasgrp4 (NM_130824) Rat Tagged ORF Clone
CAT＃: RR215109
- TrueORF®
Rasgrp4 (Myc-DDK-tagged ORF) - Rat RAS guanyl releasing protein 4 (Rasgrp4), (10 ug)
"NM_130824" in other vectors (3)
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CNY 8,740.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR215109 representing NM_130824
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCGGAAAGACATCAAAAGGAAGTCTCATCAGGAATGCTCTGGGAAGGCAGGAGGGCATGGTCGGC CGAGACAGGCCCGCCGCCACAAGACGTGTCCCACCCCCCGGGAAATCAGCAAGGTCATGGCTTCCCTGAA CCTGGGCGTGCTGAGTGAGAGCAGCTGCAGTGTGGATGAGCTACTGGAGGAATGCATCCGGTGCTTTGAT TCAGCTGGCAGCTTACACCGAGGGGACCACATTCTCAAGATGGTGCTTACCATGCACAGCTGGGTGCTGC CATCCTCAGACCTTGCCGCCCGCCTGCTGACTTCGTATCAGAAGGCTGCCAAAGATGCCCGGGAGCTAAG ACAGCTACAGATCTGTTACCTGGTCAGGTACTGGCTGACCCATCACCCCGAGGTAGTGCACCAGGAACCC CAGCTGGAAGCAATCATAAACCGGTTTTGGACCACTGTAGCTCAGGAGGGCAACATGGCCCAAAGGAGCC TAGGAGATGCCTCCAATCTGCTGAGTCCTGGAGGGCCCGGTCCCCCACCTCTCATGAGCAGCCCAGGCCT GGGCAAAAAGCGGAAAGTGTCCTTGCTGTTCGATCACCTGGAGACAGAGGAGCTGGCACAGCACCTTACT TACCTGGAGTTCCGGTCCTTCCAGGCCATCACGCCCCAAGACCTGCGGGGCTATGTTTTGCAGGGCTCGG TGAGAGGTTGTCCTGCTCTGGAAGGTTCTGTGGGTCTGAGCAACAGTGTGTCCCGCTGGGTACAGGTCAT GGTGCTGAGTCGTCCTGGGCCTGCGCAGCGTGCCCAGGTACTGGACAAATTCATTCGTGTGGCACAGAGG CTTCGCCAGATGCAGAATTTCAACACGCTGATGGCGGTCACGGGGGGTCTGTGTCATAGCGCCATCTCCA GACTCAAGGACTCCCACGTCCATCTGAGCCCTGACAGCACTAAGGCCCTGCTGGAGCTGACAGAGCTTCT CTCTTCCCACAACAACTATGCCTACTACCGCCGCACCTGGGCCAGCTGCACTGACTTCCGGCTGCCAGTA CTGGGTGTTCACCTCAAGGACCTGGTATCTCTGTATGAGGCCCATCCAGACAGGTTGCCAGATGGCCGCC TGCACCTACCCAAGCTGAATAGCCTCTATCTACGGCTGCAGGAGCTGGCAGCACTCCAGAGACAGCATCC CCCGTGCAGTGCCAATGAGGACCTGCTACACCTGCTGACGCTCTCCCTGGATCTCTTCTACACGGAAGAT GAGATCTACGAGCTTTCTTATGCCCGGGAGCCACGTTGTCCCAAGAGTCTGCCCCTATCCCCCTTCAAAG CGCCTGTGGTGGTAGAGTGGGCCCATGGTGTGACGCCAAAGCCAGACAGTGCGACTCTGGGTCAGCATGT GGAACAGCTAGTGGAGTCCGTGTTCAAGAACTATGACCCAGAAGGCCATGGCTCTATTTCTCTGGAGGAC TTTGAGAAGCTGTCAGCCAACTTCCCCTTTGCCTGCCATGGGCTTCACCCACCTCCCCGCCACGGGAGTG GCTCCTTCAGCAGAGAGGAGCTGACCAAGTATCTGCTCCATGCCAGCGCCATCTGCTCCAAGCTGGGCCT GGCTTTCCTGCACGCCTTCCAGGAGGTCACCTTCCGCAAGCCCACCTTCTGTCACAGCTGCAACGGCTTC GTGAGCACTGGCCCTCTCTGGGGCGTCACCAAGCGAGGCTATCGCTGTCAGGACTGTGGGCTGTGTTGTC ACAGACACTGCAGGGATCAAGTGAGAGTGGAGTGTAAGAAGAGGCCAGAGACTAAAGGTGACCCGGGTCC CCCTGGCGCACCCGGGCCAGCCACACCACTTCCTCCTACCGGCTGTGGCTCCGAGGAAGGTCTCTCCTAT ACACTCTCCCCGCATCTGGAGTCTGGTTGCCACCTTCACCATGCTTGGACTCAGACGGAGTCCTCACACT CTTCCTGGGAGCCAGAGATGGTGCCCTGCCCAGCACCAGTTTTACCATCCAAAGCTTCCTCAAAGTCCAG TGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR215109 representing NM_130824
Red=Cloning site Green=Tags(s) MNRKDIKRKSHQECSGKAGGHGRPRQARRHKTCPTPREISKVMASLNLGVLSESSCSVDELLEECIRCFD SAGSLHRGDHILKMVLTMHSWVLPSSDLAARLLTSYQKAAKDARELRQLQICYLVRYWLTHHPEVVHQEP QLEAIINRFWTTVAQEGNMAQRSLGDASNLLSPGGPGPPPLMSSPGLGKKRKVSLLFDHLETEELAQHLT YLEFRSFQAITPQDLRGYVLQGSVRGCPALEGSVGLSNSVSRWVQVMVLSRPGPAQRAQVLDKFIRVAQR LRQMQNFNTLMAVTGGLCHSAISRLKDSHVHLSPDSTKALLELTELLSSHNNYAYYRRTWASCTDFRLPV LGVHLKDLVSLYEAHPDRLPDGRLHLPKLNSLYLRLQELAALQRQHPPCSANEDLLHLLTLSLDLFYTED EIYELSYAREPRCPKSLPLSPFKAPVVVEWAHGVTPKPDSATLGQHVEQLVESVFKNYDPEGHGSISLED FEKLSANFPFACHGLHPPPRHGSGSFSREELTKYLLHASAICSKLGLAFLHAFQEVTFRKPTFCHSCNGF VSTGPLWGVTKRGYRCQDCGLCCHRHCRDQVRVECKKRPETKGDPGPPGAPGPATPLPPTGCGSEEGLSY TLSPHLESGCHLHHAWTQTESSHSSWEPEMVPCPAPVLPSKASSKSSV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_130824 |
| ORF Size | 2034 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_130824.2, NP_570837.2 |
| RefSeq Size | 2164 bp |
| RefSeq ORF | 2037 bp |
| Locus ID | 170668 |
| UniProt ID | Q8R5I4 |
| MW | 75.4 kDa |
| Gene Summary | mast cell specific guanine exchange factor [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN215109 | Rasgrp4 (untagged ORF) - Rat RAS guanyl releasing protein 4 (Rasgrp4), (10 ug) |
CNY 8,740.00 |
|
| RR215109L3 | Lenti ORF clone of Rasgrp4 (Myc-DDK-tagged ORF) - Rat RAS guanyl releasing protein 4 (Rasgrp4), (10 ug) |
CNY 10,550.00 |
|
| RR215109L4 | Lenti ORF clone of Rasgrp4 (mGFP-tagged ORF) - Rat RAS guanyl releasing protein 4 (Rasgrp4), (10 ug) |
CNY 11,590.00 |
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