Mrc2 (NM_001024687) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Endo180
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR215359 representing NM_001024687 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGACCCATCCGACCTGCCCTGGCGCCCTGGCCTCGTCACCTGCTGCGCTGCGTCTTACTTCTCGGGG GACTTCGTCTTGGCCACCCGGCAGACTCCGCCGCCGCCCTCCTGGAACCCGATGTCTTCCTCATCTTCAG CCAGGGGATGCAGGGCTGCCTGGAGGCCCAGGGTGTGCAGGTCCGAGTCATCCCAGTCTGCAATGCCAGT CTCCCTGCCCAGCGCTGGAAGTGGGTCTCCCGGAACCGACTCTTCAACCTGGGGGCCATGCAGTGCCTGG GTACCGGCTGGCCTGCCACCAACACCACAGTTTCCCTGGGCATGTATGAATGTGACCGAGAGGCCTTGAG TCTTCGCTGGCAGTGTCGTACACTAGGGGACCAGTTGTCTCTGCTTCTGGGGGCCCGTGCAAACAATGCA TCGAAGCCTGGTACCCTGGAGCGCGGCGACCAGACCCGCAGTGGCCATTGGAACATCTATGGCAGTGAAG AAGACCTATGTGCTCGACCCTACTACGAGGTCTACACCATCCAGGGAAACTCCCACGGGAAGCCGTGTAC TATCCCCTTCAAATACGACAACCAGTGGTTCCACGGCTGCACCAGCACGGGCCGGGAAGATGGGCACCTG TGGTGTGCCACCACCCAGGACTACGGCAAAGACGAGCGCTGGGGCTTCTGCCCCATCAAGAGTAACGACT GTGAGACATTCTGGGACAAAGACCAGCTGACTGACAGCTGTTACCAGTTTAACTTCCAATCCACACTGTC CTGGAGGGAGGCCTGGGCCAGCTGCGAGCAGCAGGGTGCAGACCTGTTGAGCATCACGGAGATCCACGAG CAGACCTACATCAATGGACTCCTCACGGGCTACAGCTCCACGCTGTGGATTGGCCTTAATGACTTGGACA CCAGTGGAGGCTGGCAGTGGTCTGACAACTCGCCCCTCAAGTACCTCAACTGGGAGAGTGATCAGCCGGA CAACCCAGGTGAGGAGAACTGTGGAGTGATCCGGACCGAGTCCTCAGGTGGCTGGCAGAACCATGACTGC AGCATCGCCCTGCCCTATGTCTGCAAGAAAAAACCCAACGCCACAGCCGAGCCCATCCAGCCAGACAGGT GGGCCAATGTCAAGGTGGAGTGTGACCCCAGCTGGCAGCCCTTCCAGGGCCACTGCTACCGCCTGCAGGC GGAGAAGCGCAGCTGGCAGGAGTCCAAGAGGGCGTGCCTACGGGGCGGGGGCGACCTCCTTAGCATCCAC AGCATGACTGAGCTGGAGTTCATCACCAAACAGATCAAGCAAGAGGTGGAAGAGCTATGGATTGGCCTCA ATGATTTGAAGCTGCAGATGAATTTTGAGTGGTCTGATGGGAGCCTCGTGAGTTTCACCCACTGGCACCC CTTTGAGCCCAACAACTTCCGTGACAGCCTGGAAGACTGTGTCACCATCTGGGGGCCGGAAGGACGCTGG AATGACAGTCCCTGTAACCAATCCTTGCCCTCCATCTGCAAGAAGGCAGGCCGACTGAGCCAGGGCACTG CAGAGGAGGACCATGGCTGCCGGAAGGGTTGGACATGGCATAGCCCATCCTGCTACTGGCTGGGAGAGGA CCAAGTGATCTACAGTGATGCCCGGCGTCTGTGTACTGACCATGGCTCTCAGCTGGTCACCATCACCAAC AGGTTTGAGCAGGCCTTCGTCAGCAGCCTCATCTATAACTGGGAGGGCGAGTACTTCTGGACGGCCCTCC AAGACCTCAACAGCACCGGCTCCTTCCGTTGGCTCAGTGGGGATGAAGTCATGTATACCCATTGGAACCG AGACCAGCCTGGGTACAGACGTGGGGGCTGTGTGGCTCTGGCCACGGGCAGTGCCATGGGACTGTGGGAG GTGAAGAACTGCACGTCGTTCCGGGCTCGCTACATCTGCCGACAGAGCCTGGGCACACCAGTGACACCAG AGTTGCCTGGCCCAGACCCCACACCCAGCCTCACTGGCTCCTGTCCCCAGGGCTGGGTCTCAGACCCCAA ACTCCGACACTGCTATAAGGTGTTTAGCTCAGAGCGGCTGCAGGAGAAGAAGAGTTGGATCGAGGCCCTC GGGGTTTGCCGGGAGTTGGGGGCCCAGCTGCTGAGTCTGGCCAGCTATGAGGAGGAACATTTTGTGGCCA ACATGCTCAACAAGATCTTTGGTGAGTCAGAACCTGAGAACCATGAGCAGCACTGGTTTTGGATTGGCCT GAACCGCAGAGACCCTAGAGAGGGTCACAGCTGGCGCTGGAGCGACGGTCTAGGGTTTTCCTACCACAAT TTTGCCCGGAGCCAGCACGATGACGACAATATCCGAGGCTGTGCGGTGCTGGACCTGGCCTCCCTGCAGT GGGTAGCCATGCAGTGCCAGACACAACTTGACTGGATCTGCAAGATCCCTAGAGGTGTGGATGTGCGGGA ACCAGACATTGGTCGGCAAGGCCGTCTGGAGTGGGTACGCTTTCAGGAGGCGGAGTACAAGTTTTTTGAG CACCACTCCTCGTGGGCGCAGGCACAGCGCATCTGTACGTGGTTCCAGGCAGAGCTGACCTCTGTTCACA GCCAGGCAGAACTGGACTTCCTGGGGCAAAATATGCAGAAGTTGTCCTCAGACCAGGAGCAGCACTGGTG GATCGGCCTGCACACCTCAGAGAGCGACGGACGCTTCAGGTGGTCAGATGGTTCTGTTATAAACTTTGTC TCCTGGGCACCGGGAAAACCTCGACCCATTGGCAAGGACAAGAAGTGTGTGTACATGACAGCTAGACAAG AGGACTGGGGGGACCAGAGGTGCCATACAGCCTTGCCCTACATCTGTAAACGCAGCAATAGCTCTGGAGA GACTCGGCCTCACGACCTGCCACCGTCAACCCTAGGAGGCTGCCCCTCCGGCTGGAACCAGTTCCTCAAC AAGTGTTTCCGAATCCAGGGCCAGGACCCCCAGGACAGGGTGAAATGGTCAGAGGCACAGTTCTCCTGTG AACAGCAAGAAGCCCAGCTGGTCACCATTGCAAACCCCTTAGAGCAAGCGTACATCACGGCCAGCCTCCC CAATGTGACCTTTGACCTCTGGATTGGCCTGCATGGCTCTCAGAGGGACTTCCAGTGGATTGAGCAGGAA CCTCTGCTCTATACCAATTGGGCACCAGGAGAGCCCTCTGGACCCAGCCCTGCTCCCAGTGGCACCAAGC CGACCAGCTGTGCGGTGATTCTGCACAGCCCCTCAGCCCACTTCACTGGCCGCTGGGATGATCGGAGCTG CACAGAGGAGACGCACGGCTTCATCTGCCAGAAGGGCACAGACCCCTCGCTGAGCCCATCCCCGGCAGCA GCACTCCCTGCCCCAGGCACCGAGCTCTCCTACCTCAACCGCACCTTCCGGCTGCTGCAGAAGCCACTGC GCTGGAAAGATGCTCTCCTGCTGTGTGAGAGCCGGAATGCCAGCCTGGCACACGTGCCTGATCCCTACAC CCAGGCCTTCCTTACGCAGGCTGCCCGGGGGCTGCAAGCACCACTGTGGATCGGGCTGGCCAGTGAGGAG GGCTCAAGGCGGTATTCCTGGCTCTCGGAGGAGCCTCTGAATTATGCGAGCTGGCAAGACGGGGAGCCCC AGCACACAGGGGGCTGTGCCTATGTGGATGTGGATGGAACCTGGCGCACCACCAGCTGTGACACCAAGCT GCAGGGGGCAGTGTGTGGGGTGAGCAGAGGGCCCCCTCCTCCAAGGATAAGCTACCGCGGCAGCTGTCCT CAGGGCCTGGCTGACTCGTCCTGGATTCCCTTCAGGGAGCACTGCTACTCTTTCCACACGGAGCTGCTGC TGGGCCACAAGGAGGCACTGCAGCGCTGCCAAAGAGCTGGTGGGACAGTTCTGTCCATTCTTGATGAGAT GGAGAATGTGTTTGTCTGGGAGCACCTGCAGACCGCTGAAACCCAGAGTCGGGGTGCCTGGTTGGGCATG AACTTCAATCCCAAAGGAGGCATGCTGGTCTGGCAAGACAATACAGCTGTGAACTATTCTAACTGGGGCC CCCCTGGCCTGGGCCCTAGCATGCTAAGCCACAACAGCTGCTACTGGATCCAGAGCAGCAGCGGGCTGTG GCGCCCCGGTGCTTGTACCAACGTCACCATGGGAGTTGTCTGCAAGCTCCCTAGAGTGGAGGAGAACGGC TTCCTGCCATCAGCAGCGCTTCCTGAGAACCCGGTGGCCCTGGTGGTGGTGCTGACTGCGGCAGTCCTAC TCCTCCTGGCCTTGCTGACAGGGGCCCTCATCCTCTACCGGCGCCGACAGAGTGCGGAGCGTGGGTCTTT TGAGGGGGCTCGCTACAGTCGCAGCAGCCGCTCTGGCCCGGCAGAGGCCACCGAGAAGAACATCCTGGTT TCTGACATGGAAATGAACGAACAACAAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR215359 representing NM_001024687 Red=Cloning site Green=Tags(s) MGPIRPALAPWPRHLLRCVLLLGGLRLGHPADSAAALLEPDVFLIFSQGMQGCLEAQGVQVRVIPVCNAS LPAQRWKWVSRNRLFNLGAMQCLGTGWPATNTTVSLGMYECDREALSLRWQCRTLGDQLSLLLGARANNA SKPGTLERGDQTRSGHWNIYGSEEDLCARPYYEVYTIQGNSHGKPCTIPFKYDNQWFHGCTSTGREDGHL WCATTQDYGKDERWGFCPIKSNDCETFWDKDQLTDSCYQFNFQSTLSWREAWASCEQQGADLLSITEIHE QTYINGLLTGYSSTLWIGLNDLDTSGGWQWSDNSPLKYLNWESDQPDNPGEENCGVIRTESSGGWQNHDC SIALPYVCKKKPNATAEPIQPDRWANVKVECDPSWQPFQGHCYRLQAEKRSWQESKRACLRGGGDLLSIH SMTELEFITKQIKQEVEELWIGLNDLKLQMNFEWSDGSLVSFTHWHPFEPNNFRDSLEDCVTIWGPEGRW NDSPCNQSLPSICKKAGRLSQGTAEEDHGCRKGWTWHSPSCYWLGEDQVIYSDARRLCTDHGSQLVTITN RFEQAFVSSLIYNWEGEYFWTALQDLNSTGSFRWLSGDEVMYTHWNRDQPGYRRGGCVALATGSAMGLWE VKNCTSFRARYICRQSLGTPVTPELPGPDPTPSLTGSCPQGWVSDPKLRHCYKVFSSERLQEKKSWIEAL GVCRELGAQLLSLASYEEEHFVANMLNKIFGESEPENHEQHWFWIGLNRRDPREGHSWRWSDGLGFSYHN FARSQHDDDNIRGCAVLDLASLQWVAMQCQTQLDWICKIPRGVDVREPDIGRQGRLEWVRFQEAEYKFFE HHSSWAQAQRICTWFQAELTSVHSQAELDFLGQNMQKLSSDQEQHWWIGLHTSESDGRFRWSDGSVINFV SWAPGKPRPIGKDKKCVYMTARQEDWGDQRCHTALPYICKRSNSSGETRPHDLPPSTLGGCPSGWNQFLN KCFRIQGQDPQDRVKWSEAQFSCEQQEAQLVTIANPLEQAYITASLPNVTFDLWIGLHGSQRDFQWIEQE PLLYTNWAPGEPSGPSPAPSGTKPTSCAVILHSPSAHFTGRWDDRSCTEETHGFICQKGTDPSLSPSPAA ALPAPGTELSYLNRTFRLLQKPLRWKDALLLCESRNASLAHVPDPYTQAFLTQAARGLQAPLWIGLASEE GSRRYSWLSEEPLNYASWQDGEPQHTGGCAYVDVDGTWRTTSCDTKLQGAVCGVSRGPPPPRISYRGSCP QGLADSSWIPFREHCYSFHTELLLGHKEALQRCQRAGGTVLSILDEMENVFVWEHLQTAETQSRGAWLGM NFNPKGGMLVWQDNTAVNYSNWGPPGLGPSMLSHNSCYWIQSSSGLWRPGACTNVTMGVVCKLPRVEENG FLPSAALPENPVALVVVLTAAVLLLLALLTGALILYRRRQSAERGSFEGARYSRSSRSGPAEATEKNILV SDMEMNEQQE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001024687
ORF Size	4440 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001024687.1, NP_001019858.1
RefSeq Size	4443 bp
RefSeq ORF	4443 bp
Locus ID	498011
UniProt ID	Q4TU93
MW	167 kDa
Gene Summary	May play a role as endocytotic lectin receptor displaying calcium-dependent lectin activity. Internalizes glycosylated ligands from the extracellular space for release in an endosomal compartment via clathrin-mediated endocytosis. May be involved in plasminogen activation system controlling the extracellular level of PLAUR/PLAU, and thus may regulate protease activity at the cell surface. May contribute to cellular uptake, remodeling and degradation of extracellular collagen matrices (By similarity). May participate in remodeling of extracellular matrix cooperating with the matrix metalloproteinases (MMPs) secreted by hepatic stellate cells. May mediate endocytosis of partially degraded collagens and glycoproteins produced in the extracellular matrix by MMPs.[UniProtKB/Swiss-Prot Function]

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