Elovl7 (NM_001191844) Rat Tagged ORF Clone
CAT#: RR216234
- TrueORF®
Elovl7 (myc-DDK-tagged) - Rat ELOVL fatty acid elongase 7 (Elovl7)
"NM_001191844" in other vectors (1)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR216234 representing NM_001191844
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTTCAGCGATCTTACATCGAGGACTGTGCGTTTTTATGATAATTGGATCAAAGATGCTGATCCAA GAGTTGAAAACTGGCTCCTCATGTCCTCGCCTCTGCCACAAACCATCATCCTGGGGCTCTACGTCTATTT TGTTACATCTCTGGGACCAAAGCTCATGGAGAACCGGAAGCCCTTTGAACTCAAGAAAGCGATGATAACG TACAATTTTTTCATAGTACTGTTTTCTGTGTATATGTGCTATGAGTTTGTGATGTCTGGCTGGGGTACAG GTTATTCGTTTCGATGCGACATTGTGGACTATTCTCAGTCGCCAAGAGCAATGAGGATGGTACACACCTG CTGGCTTTATTACTTCTCCAAATTTATCGAGCTGTTTGACACTATCTTTTTTGTTCTGCGCAAGAAAAAT AGCCAAGTGACTTTCCTTCATGTCTTCCATCACACGATCATGCCATGGACCTGGTGGTTTGGAGTCAAAT TTGCTGCAGGTGGTTTGGGGACATTCCACGCCCTTTTAAATACAGCTGTGCACGTGGTCATGTATTTCTA CTATGGACTGTGTGCAATGGGACCAGCCTACCAGAAGTATTTATGGTGGAAAAAGCATCTGACCTCTTTG CAGCTTGTCCAGTTTGTTCTTGTCACTGTCCACATAGGACAGATCTTCTTCATGGAAGACTGCAATTACC AGTACCCAGTTTTCCTGTATATCATCATGAGCTATGGATGCATCTTCCTGCTACTCTTTCTCCACTTTTG GTACCGTGCTTACACCAAGGGTCAAAGGTTGCCCAAAACTATGGAAAACGGGAATTGCAAAAGCAAGCAC CAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR216234 representing NM_001191844
Red=Cloning site Green=Tags(s) MAFSDLTSRTVRFYDNWIKDADPRVENWLLMSSPLPQTIILGLYVYFVTSLGPKLMENRKPFELKKAMIT YNFFIVLFSVYMCYEFVMSGWGTGYSFRCDIVDYSQSPRAMRMVHTCWLYYFSKFIELFDTIFFVLRKKN SQVTFLHVFHHTIMPWTWWFGVKFAAGGLGTFHALLNTAVHVVMYFYYGLCAMGPAYQKYLWWKKHLTSL QLVQFVLVTVHIGQIFFMEDCNYQYPVFLYIIMSYGCIFLLLFLHFWYRAYTKGQRLPKTMENGNCKSKH H TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001191844 |
ORF Size | 843 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001191844.1, NP_001178773.1 |
RefSeq Size | 1128 bp |
RefSeq ORF | 846 bp |
Locus ID | 361895 |
UniProt ID | D4ADY9 |
MW | 33.5 kDa |
Gene Summary | Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme with higher activity toward C18 acyl-CoAs, especially C18:3(n-3) acyl-CoAs and C18:3(n-6)-CoAs. Also active toward C20:4-, C18:0-, C18:1-, C18:2- and C16:0-CoAs, and weakly toward C20:0-CoA. Little or no activity toward C22:0-, C24:0-, or C26:0-CoAs. May participate in the production of saturated and polyunsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN216234 | Elovl7 (untagged) - Rat ELOVL fatty acid elongase 7 (Elovl7) |
CNY 2,760.00 |