Rgs6 (NM_019342) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR216747 representing NM_019342 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAGGGATCCGGAGACCAGCGAGCAGTGGGGGTTGCTGATCCGGAAGAGAGTTCTCCCAACATGA TTGTCTACTGCAAAATCGAGGACATCATTACGAAGATGCAAGATGACAAGACAGGGGGTGTGCCCATCAG AACAGTTAAGAGCTTTCTCTCCAAAATCCCCAGTGTCGTCACAGGTACTGACATTGTGCAGTGGCTTATG AAGAACCTTTCCATTGAAGACCCAGTTGAAGCAATACACCTGGGAAGCCTTATCGCCGCCCAGGGCTACG TCTTCCCAATCTCAGACCACGTCCTCACCATGAAGGACGATGGCACCTTTTACCGTTTCCAGGCTCCTTA CTTCTGGCCTTCAAACTGCTGGGAACCTGAAAACACAGACTATGCCATCTACCTCTGTAAGAGGACGATG CAGAACAAGGCAAGGCTGGAATTGGCCGACTACGAAGCAGAAAACTTAGCGAGACTCCAGAGGGCCTTTG CAAGGAAGTGGGAATTCATCTTTATGCAAGCAGAAGCGCAAGTCAAGATTGACCGGAAGAAAGATAAGAC GGAAAGGAAAATCTTGGATAGCCAAGAACGGGCCTTCTGGGATGTCCACAGGCCAGTGCCAGGCTGTGTG AACACAACGGAAATGGATATCAGAAAATGTCGGCGTTTGAAGAATCCACAAAAGGTTAAAAAGTCAGTAT ATGGTGTGACAGACGAGTCCCAGTCACAGAGTCCGGTGCACATACCAAGCCAGCCAATCAGGAAAACTAC AAAAGACGACATCCGAAAACAGATAACGTTTTTGAACGCACAGATTGACAGACATTGTTTGAAAATGTCC AAAGTGGCTGAAAGCTTAATCGCTTACACGGAGCAGTATGTGGAATACGACCCTTTCATAACACCAGCAG AGCCATCTAACCCTTGGATCAGCGATGACATCACCTTATGGGACATAGAGATGAGCAAAGAGCCCAGCCA GCAGCGAGTGAAGCGTTGGGGCTTCTCTTTTGATGAGATACTGAAGGACCAGGTGGGGCGGGACCAGTTC CTCAGATTCCTGGAGTCAGAATTCAGTTCAGAAAATCTCAGGTTCTGGCTGGCCGTCCAAGATCTCAAGA AGCAACCTCTACAGGATGTGGCCAAGAGGGTGGAGGAAATCTGGCAAGAGTTCCTAGCTCCCGGAGCCCC AAGTGCAATCAACCTGGATTCTCACAGCTATGAGATAACCAGTCAGAATGTCAAAGATGGAGGGAGATAC ACATTTGAAGATGCCCAGGAGCACATCTACAAGCTGATGAAGAGTGACAGCTACGCCCGCTTCCTACGGT CCAATGCTTACCAGGATCTGTTGCTGGCCAAGAAGAAGGGAAAGTCGCTGGCGGGCAAGCGCCTTACGGG CCTGATGCAGTCCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR216747 representing NM_019342 Red=Cloning site Green=Tags(s) MAQGSGDQRAVGVADPEESSPNMIVYCKIEDIITKMQDDKTGGVPIRTVKSFLSKIPSVVTGTDIVQWLM KNLSIEDPVEAIHLGSLIAAQGYVFPISDHVLTMKDDGTFYRFQAPYFWPSNCWEPENTDYAIYLCKRTM QNKARLELADYEAENLARLQRAFARKWEFIFMQAEAQVKIDRKKDKTERKILDSQERAFWDVHRPVPGCV NTTEMDIRKCRRLKNPQKVKKSVYGVTDESQSQSPVHIPSQPIRKTTKDDIRKQITFLNAQIDRHCLKMS KVAESLIAYTEQYVEYDPFITPAEPSNPWISDDITLWDIEMSKEPSQQRVKRWGFSFDEILKDQVGRDQF LRFLESEFSSENLRFWLAVQDLKKQPLQDVAKRVEEIWQEFLAPGAPSAINLDSHSYEITSQNVKDGGRY TFEDAQEHIYKLMKSDSYARFLRSNAYQDLLLAKKKGKSLAGKRLTGLMQSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_019342
ORF Size	1416 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_019342.1, NP_062215.1
RefSeq Size	2337 bp
RefSeq ORF	1419 bp
Locus ID	54295
MW	54.5 kDa
Gene Summary	activates G-protein alpha GTPase activity; may play a role in negative regulation of G-protein signaling; human homolog may induce neuronal differentiation [RGD, Feb 2006]

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