Tmem117 (NM_001271239) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1562562
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR216823 representing NM_001271239 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTAAAGACTTCCGTTACTATTTCCAGCATCCTTGGTCTCGCATGATCGTGGCTTACCTGGTCATCT TCTTTAACTTCTTGATATTCGCGGAGGACCCAGTCTCTCACAGCCAAACAGAAGCCAATGTTATTGTTGT TGGAAACTGTTTTTCGTTCGTAACAAATAAATACCCTAGAGGAGTTGGCTGGAGGATTTTGAAGGTGCTT CTATGGCTACTGGCCATTCTCATAGGACTGATTGCTGGCAAGTTTCTGTTCCATCAGCGTTTATTTGGTC AGTTGCTCCGGTTAAAAATGTTCCGTGAAGACCACGGGTCGTGGATGACGATGTTCTTCAGCACGATTCT GTTTCTCTTCATCTTCTCTCACATATACAACACAGTCCTTCTGATGGACGGGAACATGGGAGCATATCTC ATCACAGACTACATGGGCATCCGGAATGAAAGTTTCATGAAACTAGCTGCTGTAGGAACCTGGATGGGGG ACTTCGTCACAGCCTGGATGGTCACCGATATGATGCTTCAGGACAAGCCCTACCCTGACTGGGGGAAGTC TGCAAGAGCTTTCTGGAAGAAAGGCAACGTGCGGATCATTTTGTTCTGGACTGTCCTCTTCACCCTGACC TCCGTGGTGGTACTTGTCATCACAACCGACTGGATCAGCTGGGACAAACTGAACCGTGGATTTTTGCCCA GTGATGAGGTTTCCAGGGCCTTCCTCGCTTCTTTCATCTTGGTCTTTGACCTCCTTATCGTGATGCAGGA CTGGGAATTCCCACACTTCATGGGCGATGTTGACGTGAACCTCCCCGGGTTGCACACTCCTCACATGCAG TTCAAGATTCCCTTCTTCCAGAAGATCTTCAAGGAGGAATACCGCATTCACATAACAGGTAAATGGTTTA ACTACGGAATCATCTTCCTCGTCTTGATTTTGGACCTGAACATGTGGAAGAACCAAATATTTTACAAACC TCATGAATATGGACAGTATATTGGCCCAGGGCAGAAGATATACACAGTGAAGGACTCTGAAAGTTTAAAG GATTTAAACAGAACCAAGCTATCCTGGGAATGGCGGTCCAATCACACTAATCCTCACACTAACAAAACCT ATGTTGAAGGCGACATGTTCTTACACAGCAGGTTCACAGGGGCCAGCCTAGATGTCAAGTGTCTGGCTTT TGTTCCAAGTCTGATAGCTTTCGTGTGGTTTGGATTCTTTATTTGGTTCTTTGGACGGTTTCTAAAGAAC GAGCAAGGGATGGAGAATCAAGATAAAACTTACACTCGCATGAAAAGAAAATCACCCTCGGAACACAGCA AAGACATGGGCATCACTCGAGAAAGCACCCAGGTCTCCGTGGAAGACCCGCTGAACGACCCGCCCTTGGT TTGCATCAGGTCCGACTTTAACGAGATAGTCTACAAGTCCTCCCATCTGACATCGGAAAACTTGAGCCTG CATCTGAAGGAGTCTACCAGTGCGGCGGAGGCCGATCAAGACCCAGCGGCCTCTCCGAGTCTGCGGACGG AC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR216823 representing NM_001271239 Red=Cloning site Green=Tags(s) MGKDFRYYFQHPWSRMIVAYLVIFFNFLIFAEDPVSHSQTEANVIVVGNCFSFVTNKYPRGVGWRILKVL LWLLAILIGLIAGKFLFHQRLFGQLLRLKMFREDHGSWMTMFFSTILFLFIFSHIYNTVLLMDGNMGAYL ITDYMGIRNESFMKLAAVGTWMGDFVTAWMVTDMMLQDKPYPDWGKSARAFWKKGNVRIILFWTVLFTLT SVVVLVITTDWISWDKLNRGFLPSDEVSRAFLASFILVFDLLIVMQDWEFPHFMGDVDVNLPGLHTPHMQ FKIPFFQKIFKEEYRIHITGKWFNYGIIFLVLILDLNMWKNQIFYKPHEYGQYIGPGQKIYTVKDSESLK DLNRTKLSWEWRSNHTNPHTNKTYVEGDMFLHSRFTGASLDVKCLAFVPSLIAFVWFGFFIWFFGRFLKN EQGMENQDKTYTRMKRKSPSEHSKDMGITRESTQVSVEDPLNDPPLVCIRSDFNEIVYKSSHLTSENLSL HLKESTSAAEADQDPAASPSLRTD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271239
ORF Size	1542 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271239.1, NP_001258168.1
RefSeq Size	2781 bp
RefSeq ORF	1545 bp
Locus ID	500921
MW	60.1 kDa

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