Fance (NM_001191718) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1561045
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR216893 representing NM_001191718 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACCTGGGGCGCTCCGACCCCGAGTGTCCTGTCCGCCGCCCCGCCTCGGCTCTCCGCTGGCGAGGC CAGTGTCGGACGCCGTGGCCTCGGCCAGGGCGACGCCATGGGCGACCCTGGAGGCCCCCGCCCGTCTCCT CCTGCAGGCGCTGCAGGCCGGACCCGAGGGTGCGCGGCGCGGGCTAGGGGTTCTGCGCACCTTGGGCCGC CGCGCTGAGCACTTCCCTTGGGATAGCTTCCTGGAGGCGCTGGGCCGCGCAGAGCCCGAAGTGCGGGGCC CAGATGGCCGCCTGGAACTGACGCCACTGTTGCTACGGTTGCCTGGAGTATGCCGGAAGAACCTGATGTC CTTGCTGCTGGCTGTTCGATCCTCCTTACCCGAAAGCGGCCTCCGCTCTGTGCTGCAGCTTGATCAGCAG GGCACAGCATCTACCTCTGATGCCTGGCTCCACGCCCTCAGGGAGCTGCTGCAAAGGGATGTGGGGCTTG GAGTCTCTGTGGAGGGATCCTCCCCATTGACCGGAAGCTGTCAGTCACATCTCCGGGACCTGTGTGGGCG GCTGGGCCAAGGTGGGAGGGGGCTGAAACCGGCCCTGGGTTCTGATCCTGAACCAGAGGGCAGACTCTCA CAGCTTTCTGGGAAGCGGAGGAAAAGGACCGAGGGAGAGACTGCCGGCCCTGAGTTAGAGAGATCCCCTA AAAGGTTCCGGGGTTGTGAGGAGGAACAGGAAGAGGTGGTGGAAAGAAAGGAAGAGAGACCCAAGCCAGA GACTTCGGAATCAGAGTCAGATGCAGGAGGCGTGTTGCCTGATACTGAAGTCGAGGCTCCGGAGGCTCGC CCGGGCGTAGCAGAGGCCGAGAGTCCAGCTAAGAGTGTGGAGCTCCCCAAAGTTGTCCAGGACCAGGTGC CCAGGCTGCGGCAGCTGCTGAAGGCCTTGCAGGAGGGGCTGGAGGGGCTGGAGGAGCCCCCAGTGGACCT GCAGTTTCTTCACGAATGTAGTCCCAGCCAGATGGAGTCGCTATGCAGCCAGCTACAACTGCCCCAGCTC CCGGACGGAGGCCTCCTGCAGCTCTGCAGCCACCTGCTGGGTCTCACCCCAGCCCTCAGCATCAGCAATG CCTCTGTGCTGGCCAGGAGCCTCTTTCTTGACCGGATCCTCTCCCTGCCATCCTCCGCCTCCAGGCTTCT CAGAGCTGCCCTCACCTCCTTCTGTGCCAAGTACACCTACGCTGTCTGCAGGGCTGTCCTCTGTCCCTTG TTCCAGGACCCAGGCATAGGTCCTGTGCAGACGGAGTTACTGTGTTCCCTCTTAAAGGATGAGTCCTTGG AGCCAGACATGCAGGTCCAGATTCTGGGGCAGGTCCTGGAGCTGGCCTGGAGGGAAGAGACGTTCCTGGT GTTGCAGGCGCTCCTGGAGCGGCAGGTGGAGATGACCTCGGAGCTCTTCAGTGTCTTGATGCAGAGGCTC TGCAAAGAGGGGCCGGCAGCCACCACCTCCATGGCCTATGCCAAGCTGATGCTGACGGTGATGACCAAGT ACCAGGCCAGCATCTCACAGCAGCAGAGTCTGGACCTGGCTGTGGCCCTAGAGCCCAACACCACCTTCCT GAAGAAGTCCCTGCAGGCAGCCCTGAGACATCTGGCCCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR216893 representing NM_001191718 Red=Cloning site Green=Tags(s) MAPGALRPRVSCPPPRLGSPLARPVSDAVASARATPWATLEAPARLLLQALQAGPEGARRGLGVLRTLGR RAEHFPWDSFLEALGRAEPEVRGPDGRLELTPLLLRLPGVCRKNLMSLLLAVRSSLPESGLRSVLQLDQQ GTASTSDAWLHALRELLQRDVGLGVSVEGSSPLTGSCQSHLRDLCGRLGQGGRGLKPALGSDPEPEGRLS QLSGKRRKRTEGETAGPELERSPKRFRGCEEEQEEVVERKEERPKPETSESESDAGGVLPDTEVEAPEAR PGVAEAESPAKSVELPKVVQDQVPRLRQLLKALQEGLEGLEEPPVDLQFLHECSPSQMESLCSQLQLPQL PDGGLLQLCSHLLGLTPALSISNASVLARSLFLDRILSLPSSASRLLRAALTSFCAKYTYAVCRAVLCPL FQDPGIGPVQTELLCSLLKDESLEPDMQVQILGQVLELAWREETFLVLQALLERQVEMTSELFSVLMQRL CKEGPAATTSMAYAKLMLTVMTKYQASISQQQSLDLAVALEPNTTFLKKSLQAALRHLAR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191718
ORF Size	1650 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191718.1, NP_001178647.1
RefSeq Size	1864 bp
RefSeq ORF	1653 bp
Locus ID	309643
MW	59.7 kDa

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