Erf (NM_001170335) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR216897 representing NM_001170335 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGACCCCAGCGGACACAGGGTTTGCCTTCCCAGATTGGGCCTACAAGCCGGAGTCATCCCCTGGCT CGAGGCAGATCCAGCTGTGGCACTTTATCTTGGAGCTGCTTCGGAAAGAGGAGTACCAGGGCGTCATCGC CTGGCAGGGAGACTACGGGGAATTTGTCATCAAGGACCCTGATGAGGTGGCCCGACTCTGGGGGGTCCGC AAGTGCAAACCCCAGATGAACTACGACAAGCTGAGCCGGGCCCTGCGCTATTACTATAACAAGCGCATTC TGCACAAGACCAAGGGGAAACGGTTCACCTACAAGTTCAACTTCAACAAACTGGTGCTGGTTAATTACCC TTTCATCGATGTGGGGCTGGCCGGGGGCGCTGTTCCCCAGAGTGCCCCTCCAGTGCCATCGGGCGGCAGC CATTTCCGCTTCCCTCCTTCGACGCCCTCCGAGGTGCTGTCCCCCACCGAGGATCCCCGCTCTCCACCAG CTTGTTCTTCATCGTCCTCTTCTCTCTTCTCCGCTGTGGTTGCTCGACGCCTGGGCCGAGGCTCTGTCAG CGACTGCAGTGATGGCACCTCAGAGCTGGAGGAGCCTCTGGGGGAGGACCCCAGGGCACGACCACCTGGC CCTCCGGAGCTGGGTGCCTTCCGAGGGCCCCCGCTGGCCCGCCTCCCACATGATCCTGGTGTCTTCCGTG TCTATCCTCGGCCTCGGGGTGGTCCTGAACCCCTAAGTCCCTTCCCTGTGTCACCTTTGGCTGGGCCTGG CTCCCTTCTACCCCCTCAACTCTCCCCAGCTCTGCCCATGACTCCCACCCACCTGGCCTACACACCCTCA CCCACGCTGAGTCCTATGTACCCCAGTGGTGGCGGGGGCCCTAGTGGCTCAGGGGGGGGTTCCCACTTCT CCTTCAGTCCGGAGGACATGAAACGGTACCTGCAGGCCCACACCCAAAGCGTCTACAACTACCACCTCAG TCCCCGCGCCTTCCTGCACTATCCAGGGCTGGTGGTGCCCCAGCCTCAGCGCCCTGACAAGTGCCCACTG CCGCCCATGGCACCGGAGACCCCGCCGGTTCCCTCCTCAGCCTCGTCTTCCTCTTCTTCCTCTTCATCCC CGTTCAAGTTTAAGCTGCAGCCACCCCCGCTAGGACGCCGGCAGCGGGCAGCTGGAGAGAAGGCTCCAGG GGGCACTGACAAGAGCAGTGATGGTAGTGGCTCTGGTGGGCTGGCCGAGGGGGCAGGCGCATTAGCCCCC CCACCGCCACCACCACCCCAGATTAAGGTGGAACCCATCTCAGAGGGCGAGTCGGAGGAGGTGGAGGTGA CTGACATCAGTGACGAAGATGAGGAAGATGGGGAGGTGTTCAAGACCCCCCGTGCCCCACCCGCTCCCCC CAAGCCAGAGCCTGGAGAGGCACCAGGGGCAGCCCAGTGCATGCCTCTTAAACTGCGCTTTAAAAGGCGC TGGAGTGAAGACTGTCGCCTGGAGGGGGGCGGGTGCCTCTCTGGGGGCCCTGAGGATGAGGGTGAGGACA AGAAGGTGCGTGGGGACATGGGCCCTGGGGAGCCTGGGGGACCCCTCACTCCTCGGCGAGTGAGCTCTGA TCTCCAGCACGCTACAGCCCAGCTCTCCCTGGAGCACCGAGATTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR216897 representing NM_001170335 Red=Cloning site Green=Tags(s) MKTPADTGFAFPDWAYKPESSPGSRQIQLWHFILELLRKEEYQGVIAWQGDYGEFVIKDPDEVARLWGVR KCKPQMNYDKLSRALRYYYNKRILHKTKGKRFTYKFNFNKLVLVNYPFIDVGLAGGAVPQSAPPVPSGGS HFRFPPSTPSEVLSPTEDPRSPPACSSSSSSLFSAVVARRLGRGSVSDCSDGTSELEEPLGEDPRARPPG PPELGAFRGPPLARLPHDPGVFRVYPRPRGGPEPLSPFPVSPLAGPGSLLPPQLSPALPMTPTHLAYTPS PTLSPMYPSGGGGPSGSGGGSHFSFSPEDMKRYLQAHTQSVYNYHLSPRAFLHYPGLVVPQPQRPDKCPL PPMAPETPPVPSSASSSSSSSSSPFKFKLQPPPLGRRQRAAGEKAPGGTDKSSDGSGSGGLAEGAGALAP PPPPPPQIKVEPISEGESEEVEVTDISDEDEEDGEVFKTPRAPPAPPKPEPGEAPGAAQCMPLKLRFKRR WSEDCRLEGGGCLSGGPEDEGEDKKVRGDMGPGEPGGPLTPRRVSSDLQHATAQLSLEHRDS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001170335
ORF Size	1656 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001170335.1, NP_001163806.1
RefSeq Size	3711 bp
RefSeq ORF	1659 bp
Locus ID	292721
MW	59.2 kDa

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