Tlk2 (NM_001191652) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217166 representing NM_001191652 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGAAGAACTGCACAGCCTGGACCCACGGAGGCAGGAGTTACTGGAGGCCAGGTTCACTGGGGTTG GCGTAAGTAAGGGGCCACTCAACAGTGAGTCTTCCAACCAGAGTTTGTGCAGTGTGGGGTCCTTGAGTGA TAAAGAAGTAGAGACTCCTGAGAAAAAGCAGAATGACCAGCGAAATCGGAAAAGAAAAGCTGAACCATAT GAAACTAGCCAAGGGAAAGGCACTCCTAGGGGACATAAAATTAGTGATTACTTTGAGTTTGCTGGGGGAA GCGGGCCAGGAACCAGCCCTGGCAGAAGTGTTCCACCAGTTGCACGATCCTCACCGCAACATTCCTTATC CAATCCCTTACCGCGTCGAGTAGAACAGCCTCTCTATGGTTTAGATGGCAGTACTGCAAAGGAGGCCTCA GAAGAGCAGTCTGCCCTGCCAACCCTCATGTCAGTGATGTTAGCAAAACCTCGACTTGACACAGAGCAGT TAGCGCCAAGGGGAGCTGGCCTCTGCTTCACTTTCGTCTCTGCTCAACAAAACAGCCCTTCGTCCACGGG GTCTGGCAATACAGAACATTCTTGCAGCTCCCAGAAACAGATCTCCATCCAGCACAGGCAGACCCAGTCT GACCTCACAATAGAAAAAATATCTGCACTAGAAAACAGTAAGAACTCTGACTTAGAGAAGAAGGAAGGAA GAATAGATGATTTATTAAGAGCCAACTGTGATTTGAGACGACAGATAGATGAACAGCAAAAGATGCTAGA GAAATACAAGGAACGATTAAATAGATGTGTCACCATGAGCAAGAAGCTCCTTATAGAAAAGTCAAAACAA GAGAAGATGGCATGCAGGGACAAGAGCATGCAGGACCGATTGCGATTAGGTCACTTTACAACTGTCCGGC ATGGAGCCTCTTTTACTGAACAGTGGACAGATGGTTATGCTTTCCAAAACCTCATCAAGCAACAGGAAAG GATAAATTCACAGAGAGAAGAGATAGAAAGGCAACGGAAAATGTTAGCAAAACGGAAACCCCCTGCCATG GGTCAGGCCCCTCCCGCAACCAATGAGCAGAAACAACGGAAAAGCAAGACCAATGGAGCTGAAAATGAAA CGTTAACCTTAGCTGAATACCATGAACAAGAAGAAATCTTCAAACTTAGATTAGGTCATCTTAAGAAAGA GGAAGCAGAAATCCAGGCAGAACTGGAAAGGCTAGAAAGGGTTAGGAATCTACACATCAGGGAATTAAAA AGGATACATAATGAAGATAATTCACAGTTTAAAGACCACCCAACACTAAATGACAGATATTTGTTGTTAC ATCTTTTGGGTAGAGGAGGTTTCAGTGAAGTTTACAAGGCGTTTGATCTAACGGAGCAAAGATATGTAGC TGTGAAAATTCACCAGTTAAATAAAAACTGGAGAGATGAGAAAAAGGAGAATTACCACAAGCATGCGTGT AGGGAATACCGGATTCACAAAGAACTGGATCACCCCAGAATAGTGAAGCTGTATGATTACTTTTCACTGG ACACTGACTCGTTTTGTACAGTATTAGAATACTGTGAAGGAAATGATCTGGACTTCTACCTAAAGCAGCA CAAATTAATGTCGGAGAAAGAAGCCCGATCCATTATTATGCAGATTGTGAATGCTTTAAAGTACTTAAAT GAAATAAAACCTCCCATCATACACTATGACCTCAAACCAGGTAATATCCTTTTAGTAAATGGTACAGCGT GTGGAGAGATAAAAATTACAGATTTTGGTCTTTCCAAGATCATGGATGACGATAGCTACAATTCAGTGGA CGGCATGGAGCTAACGTCACAAGGTGCTGGTACTTATTGGTATTTGCCACCAGAGTGTTTTGTGGTTGGG AAAGAGCCACCAAAGATCTCAAATAAAGTCGATGTCTGGTCAGTGGGTGTGATCTTCTACCAGTGTCTGT ATGGGAGGAAGCCTTTTGGTCATAACCAGTCCCAGCAAGATATTCTACAAGAGAATACTATTCTTAAAGC TACTGAAGTACAGTTCCCGCCCAAGCCAGTAGTAACACCTGAAGCAAAGGCGTTTATCAGGCGATGTTTG GCCTATCGAAAGGAAGACCGCATTGATGTGCAGCAGCTGGCCTGTGACCCCTACTTGTTGCCTCACATCC GAAAGTCAGTCTCCACAAGTAGTCCTGCAGGAGCTGCCATTGCATCAACCTCGGGGGCATCCAATAACAG TTCTTCGAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217166 representing NM_001191652 Red=Cloning site Green=Tags(s) MMEELHSLDPRRQELLEARFTGVGVSKGPLNSESSNQSLCSVGSLSDKEVETPEKKQNDQRNRKRKAEPY ETSQGKGTPRGHKISDYFEFAGGSGPGTSPGRSVPPVARSSPQHSLSNPLPRRVEQPLYGLDGSTAKEAS EEQSALPTLMSVMLAKPRLDTEQLAPRGAGLCFTFVSAQQNSPSSTGSGNTEHSCSSQKQISIQHRQTQS DLTIEKISALENSKNSDLEKKEGRIDDLLRANCDLRRQIDEQQKMLEKYKERLNRCVTMSKKLLIEKSKQ EKMACRDKSMQDRLRLGHFTTVRHGASFTEQWTDGYAFQNLIKQQERINSQREEIERQRKMLAKRKPPAM GQAPPATNEQKQRKSKTNGAENETLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELK RIHNEDNSQFKDHPTLNDRYLLLHLLGRGGFSEVYKAFDLTEQRYVAVKIHQLNKNWRDEKKENYHKHAC REYRIHKELDHPRIVKLYDYFSLDTDSFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIIMQIVNALKYLN EIKPPIIHYDLKPGNILLVNGTACGEIKITDFGLSKIMDDDSYNSVDGMELTSQGAGTYWYLPPECFVVG KEPPKISNKVDVWSVGVIFYQCLYGRKPFGHNQSQQDILQENTILKATEVQFPPKPVVTPEAKAFIRRCL AYRKEDRIDVQQLACDPYLLPHIRKSVSTSSPAGAAIASTSGASNNSSSN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191652
ORF Size	2250 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191652.2
RefSeq Size	3689 bp
RefSeq ORF	2253 bp
Locus ID	303592
MW	85.4 kDa
Gene Summary	mouse homolog is a serine threonine kinase; may have a role in phosphorylations involved in regulating spermatogenesis [RGD, Feb 2006]

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