Il12rb2 (NM_001191750) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217281 representing NM_001191750 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACACACTGTTAGAGGATGCTCATTGGCACTTATTTTTTTGTTCATGTGGCTGTTGATTAAAGCAA ATATAGATGTGTGCAAGCTTGGCACCGTGACTGTCCAGCCTGCCCCTGTGATTCCACTTGGGTCAACTGC CAATATTTCCTGCTCTTTGAATCCCAAACAAGGCTGTTCGCATTATCCTAGTTATAACGAATTAATCCTC TTAAAGTTTGTCAATGATGTCCTTGTCGAAAAACTCCATGGCAAAAAAGTCCATGGCCACCCTAGTCATT CCTCCATATTTCAAGTCACCAACCTGTCTCTTGGTATGACTCTGTTTGTCTGCAAGCTAAACTGTAGCAA CTCTCAAAAGACACAACCAGTGCCAGTGTGTGGAGTGGAGATCTCAGTCGGTGTTGCTCCAGAGTCACCT CAAAGCATATCATGTGTCCAGAAAGGAGAGCATGGAACTGTGGCCTGCTCCTGGAACTCTGGGAAAGTTA CTTATCTGAAAACCTATTACACTTTACAGTTAACTGGACCAAACAATGTGACCTATCAGAACCAGTGTTA TTCTGACAATCGTCAGAATTGTAGTCGTCTGGATCTTGGGATCAATCTGACCCCTGATTTAGCCGAATCC AGGTTCATAGTCCATGTTACTGCCACCAATGATCTTGGAAATTCTTCTTCACTTCTGCATACATTCACAT TCTTGGACATAGTGAGGCCTCTTCCTCCGTGGGACATCAGAATCAACTTTCTAAATGCTTCTGGGAGCAG ATGTACACTGCAGTGGGAAGATGAGGGGCAAGTGGTACTCAATCGACTCAGATACCAGCCTCTTAACAGC ACATCCTGGAATATGGTTAATGCTACAAATGCCAAAGGAAGATATGACCTGCAAGATCTGAGACCATTCA CAGAATATGAATTTCAGATCTCCTCTAAGCTACATCTCTCTCAGGGAAGTTGGAGTAATTGGAGTGAGTC ACTGAGAACTCGAACACCAGAGGAAGAGCCTGTTGGGACATTAGACATCTGGTACATGAAACAAGATATT GACTGTGACAGACAACAGATCTCTCTTTTCTGGAAGAGTCTGAATCCATCAGAGGCAAGGGGGAAGATCC TCCACTATCAGGTGACTTTACAGGAGGTGACGAAGAAAACTACACTGCAGAATACTACAAGACATACCTT CTGGACCGGGGTCATTCCCAGAACTGGGGCTTGGACTGCATCTGTGTCTGCAGCCAACTCAAAAGGCACT TCTGCACCCACTCACATTAACATAGTGGACCTGTGTGGCACTGGGTTCCTGGCTCCTCACCAGGTCTCTG CAAAGTCAGGGAACATGGACAACATCATGGTGACCTGGCAGCCTCCTGGGAAAGCTGACTCTGTGATTCG GGAGTACATAGTGGAATGGAGGGCTCTCCAACAAGGGAGCGTCATGAACCTTACCCCACACTGGCTGCGA ATTCCCCCCTACAACATGTCTGCTCTGATTTCAGAGAAGATAAAGCCCTATATCTGTTACGAAATCCGGG TACATGCACTGTCAGAGGACCAAGGAGGGTGCAGCTCCATCCGGGGTGACTCCAGTCACAAAGTGCCACT GAGTGGCCCTCATATCACTGCTATCACAGAGAAAAAGGAAAGCCTTTTTATTTCCTGGACCCACATCCCT TTCCTGGAGCAAAGGGGCTGCATCCTCCATTACAGAATATATTGGAAGGAGCGACACTCTACTGCGCAAC CCGAGCTCTGTGAAATTCAGTACAGACACTCTCAAAACTCACATCCAATAAGCAGCCTACAGCCCAAGGT GACATATGTCCTATGGATGACAGCTGTGACAGCTGCTGGTGAAAGTCCCCAAGGAAATGAAAGGGAATTT TGTCCACAGGGCAAAGCCAACTGGAAAACATTCGTGATATCAAGCATTTGCATCGCTATCATCGTGGTGG GCACTTTCTCAATTCGTTACTTCAGGCAAAAGGCATTTACTCTCCTTTCTACTCTCAAACCCCAATGGTA TTGCAGAACTATTCCAGATCCAGCAAATAGCACTTGGGTAAAGAAGTACCCCATTATGGAGGAGAAGATC CAGCCACCTATGGACAATCTCCTGATGGCCTGGTCCGCTCCTGAAGAGCCTGAGCCCCTGATCATCAATG AAGTCCTCTACCAAATGATCCCAGTAGGCAGACAACCCTATTGCTTCAAAAGAGGACAAGGGTTCCAAGG TTACTCTACCTCCAAGGAAGACACAATATACCTTGCCACCAATCCACAAACTACAGGAACTCTCACAGCT GAGTCGAGACAACTAGTGAATCTATACAAGGTGCTAGGAAGCAGGGACCCTGACTCAAAACTGGGCAACC TGACCAGCCCCTTGACAGTCACCCCAGTGAACTACCTTCCTACCCATGAAGGCTATTTACCCTCCAACAT AGAAGACCTCTCATCACATGAAGTTGACCCAAGTGATTCTTTAGACCTGGACCATCAACATATTTCTCTT TCCATTTTTGCATCAAGTTCTCTCCGCCCACTCACCTTCTGTGGTGAGAGGCTGACTCTAGATCGGTTAA AGATGGGCTACGAGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217281 representing NM_001191750 Red=Cloning site Green=Tags(s) MAHTVRGCSLALIFLFMWLLIKANIDVCKLGTVTVQPAPVIPLGSTANISCSLNPKQGCSHYPSYNELIL LKFVNDVLVEKLHGKKVHGHPSHSSIFQVTNLSLGMTLFVCKLNCSNSQKTQPVPVCGVEISVGVAPESP QSISCVQKGEHGTVACSWNSGKVTYLKTYYTLQLTGPNNVTYQNQCYSDNRQNCSRLDLGINLTPDLAES RFIVHVTATNDLGNSSSLLHTFTFLDIVRPLPPWDIRINFLNASGSRCTLQWEDEGQVVLNRLRYQPLNS TSWNMVNATNAKGRYDLQDLRPFTEYEFQISSKLHLSQGSWSNWSESLRTRTPEEEPVGTLDIWYMKQDI DCDRQQISLFWKSLNPSEARGKILHYQVTLQEVTKKTTLQNTTRHTFWTGVIPRTGAWTASVSAANSKGT SAPTHINIVDLCGTGFLAPHQVSAKSGNMDNIMVTWQPPGKADSVIREYIVEWRALQQGSVMNLTPHWLR IPPYNMSALISEKIKPYICYEIRVHALSEDQGGCSSIRGDSSHKVPLSGPHITAITEKKESLFISWTHIP FLEQRGCILHYRIYWKERHSTAQPELCEIQYRHSQNSHPISSLQPKVTYVLWMTAVTAAGESPQGNEREF CPQGKANWKTFVISSICIAIIVVGTFSIRYFRQKAFTLLSTLKPQWYCRTIPDPANSTWVKKYPIMEEKI QPPMDNLLMAWSAPEEPEPLIINEVLYQMIPVGRQPYCFKRGQGFQGYSTSKEDTIYLATNPQTTGTLTA ESRQLVNLYKVLGSRDPDSKLGNLTSPLTVTPVNYLPTHEGYLPSNIEDLSSHEVDPSDSLDLDHQHISL SIFASSSLRPLTFCGERLTLDRLKMGYEK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191750
ORF Size	2607 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191750.1, NP_001178679.1
RefSeq Size	2610 bp
RefSeq ORF	2610 bp
Locus ID	171334
MW	97.7 kDa
Gene Summary	transmembrane protein that belongs to the hemopoietin receptor superfamily; human homolg binds interleukin (IL)12 with a low affinity and may be a part of the IL12 receptor complex [RGD, Feb 2006]

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