Ect2 (NM_001108547) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217314 representing NM_001108547 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACGACAGTGTGTTACCATCTCCTACTGAGATAACTAGCTTGGCAGACACTTCAGTTTTTGATT CTAAAGTTACAGAAACATCCAAGGAAAACTTGTGCATGGTGTCTACTTCAAATGTTGACGAAGAAATGCC TCAGGTGGAAGCAAGAGTGGTAGTGGTCCAGGAAGCTGGGAAACAAGAGGAGCTCCTAAAGGCCTTAAAG ACTGTTAAGATAATGGAAGTCCCTGTTATAAAGATAAAAGAAAGTTGTCCTGGGAAATCGGATGAAAAGT TAATAAAAAGTATTATTAATATGGAAATTAAAGTGCCCTGTGTAAAGATGGATTCAATGGAAGAATTTGG AAGTTTGGATTCCCCAGAATTTGAGAATATATTTGTAGTTACTGACTTCCAGACATCTGTCTTCAATGAC TTATACAAGGCTGATTGTAGGATTGTTGGACCACCAGTTATACTCAACTGTGCACAAATGGGAGAGCCCT TGCCGTTCTCCTGCCGGCCGCTGTACTGCACGAGCATGCTGAATCTGGTGCTCTGCTTCACTGGATTCAG GAAGAAGGAGGAGCTCGTCAAATTGGTGACATTGGTTCATCATATGGGTGGAGTTATTCGAAAAGAATGC AATTCCAAAGTAACACATCTGGTGGCAAATTGTACGCAAGGAGAAAAGTTTAGGGTTGCTGTGAGCCTGG GGACTCCAATTATGAGGCCAGGATGGATTTATGAAGCATGGGAAAGACGCAATGAACAGTGTTTCTGTGC AGCAGATGATGACTTTAGAAATGAATTTAAAGTTCCTCCATTCCAAGATTGCATTTTAAGTTTTCTGGGA TTTTCAGATGAAGAGAAACACAGTATGGAAGAAATGACTGAAATGCAAGGAGGTACCTATTTACCAGTTG GGGATGAAAGGTGCACTCACCTTATTGTTGATGAGAACACAGTAAAGGAACTTCCATTTGAACCTTCCAA GAAACTTTTTGTGGTCAAGCAAGAGTGGTTCTGGGGAAGCATTCAGATGGATGCCCGGGCTGGTGAAACT ATGTATTTGTATGAAAAGGCTAATACTCCTGAGCTCAAGAAATCCGTGTCTTTGCTGTCTCTGAGTACTC CAAACAGCAACCGCAAAAGACGGAGGTTGAAAGAAAGCCTGGCTCAGCTCTCCAGAGAGACCGACCTCTC TCCTTTCCCTCCGCGCAAGCGGCCCTCAGCAGAGCATTCCCTTTCTATCGGCTCGCTCCTGGATATCTCC AACACACCGGACTCCAGCATTCACTATGGAGAAACACCGAAGTCTTGTGCTAAGTCTTCCAGGAGTTCAA CTCCAGTTCCTCCAAAGCAGTCGGCCAGGTGGCAGGTGGCAAAAGAGCTCTACCAGACTGAGAGCAACTA TGTCAATATACTGGCCACCATCATTCAATTATTTCAGGTACCCTTAGAAGAAGAAGGACAACGGGGTGGG CCTATTCTTGCACCCGAGGAAATCAAGACTATTTTTGGGAGCATCCCAGACATCTTTGACGTACATATGA AGATAAAGGATGATCTTGAAGACCTTATTGCTAACTGGGATGAGAGCAGAAGTATTGGTGACATCTTTCT TAAATATTCCAAAGATTTGGTAAAAACCTATCCTCCATTTGTAAACTTCTTTGAAATGAGCAAGGAAATG ATTATTAAATGTGAAAAACAGAAGCCCAGATTTCATGCTTTTCTCAAGATAAATCAAGCTAAACCAGAGT GTGGACGGCAAAGCCTTGTGGAACTTCTCATTCGGCCAGTACAGCGGTTACCCAGTGTTGCCTTACTTTT AAACGATCTTAAAAAGCATACAGCTGATGAAAATCCAGACAAAAGCACGTTAGAAAAAGCTATTGGATCA CTAAAAGAAGTAATGACACATATCAATGAGGATAAGAGAAAAACAGAAGCACAGAAGCAAATTTTTGATG TTGTTTATGAAGTAGATGGATGCCCAGCTAATCTATTATCTTCTCATCGAAGCCTGATACAAAGAGTGGA AACAGTTTCTCTTGGTGAGCACCCCTGTGACCGAGGAGAACAAGTCACTCTCTTCCTCTTCAATGACTGC CTTGAGATAGCAAGAAAACGGCACAAGGTTATTGGCACTTTTAGAAGTCCTCATGACCGCACCCGGCCAC CAGCTTCTCTGAAGCACATTCATCTAATGCCTCTTTCTCAGATTAAGAAGGTGCTGGACATCAGAGAGAC AGAAGATTGTCATAATGCCTTTGCCTTGCTTGTGAGGCCACCAACAGAGCAGGCCAACGTGCTGCTCAGC TTCCAGATGACGTCAGAGGAGCTTCCAAAGGAGAGCTGGCTGAAGATGCTGTGTCGACATGTTGCCAACA CCATTTGTAAAGCAGATGCTGAGAATCTTATGTACGTGACTGATCCAGAATCTTTTGAAGTAAATACAAA AGATATGGATAGTACACTTAGTCGAGCATCTAGAGCAATAAAAAAGACTTCAAAAAAGGTTACAAGGGCA TTCTCTTTTTCCAAAACTCCCAAGAGAGCCCTGCGGATGGCTCTGTCATCATCCCATAGCTCAGAGGGAA GGAGTCCTCCGAGTAGTGGCAAACTTGCTGTGAGCCGTCTGTCCAGCACATCTTCCTTAGCAGGTATTCC ATCGCCTTCCCTTGTCAGCCTCCCTTCTTTCTTTGAACGGAGAAGTCATACATTAAGTCGATCTACAACT CACTTGATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217314 representing NM_001108547 Red=Cloning site Green=Tags(s) MADDSVLPSPTEITSLADTSVFDSKVTETSKENLCMVSTSNVDEEMPQVEARVVVVQEAGKQEELLKALK TVKIMEVPVIKIKESCPGKSDEKLIKSIINMEIKVPCVKMDSMEEFGSLDSPEFENIFVVTDFQTSVFND LYKADCRIVGPPVILNCAQMGEPLPFSCRPLYCTSMLNLVLCFTGFRKKEELVKLVTLVHHMGGVIRKEC NSKVTHLVANCTQGEKFRVAVSLGTPIMRPGWIYEAWERRNEQCFCAADDDFRNEFKVPPFQDCILSFLG FSDEEKHSMEEMTEMQGGTYLPVGDERCTHLIVDENTVKELPFEPSKKLFVVKQEWFWGSIQMDARAGET MYLYEKANTPELKKSVSLLSLSTPNSNRKRRRLKESLAQLSRETDLSPFPPRKRPSAEHSLSIGSLLDIS NTPDSSIHYGETPKSCAKSSRSSTPVPPKQSARWQVAKELYQTESNYVNILATIIQLFQVPLEEEGQRGG PILAPEEIKTIFGSIPDIFDVHMKIKDDLEDLIANWDESRSIGDIFLKYSKDLVKTYPPFVNFFEMSKEM IIKCEKQKPRFHAFLKINQAKPECGRQSLVELLIRPVQRLPSVALLLNDLKKHTADENPDKSTLEKAIGS LKEVMTHINEDKRKTEAQKQIFDVVYEVDGCPANLLSSHRSLIQRVETVSLGEHPCDRGEQVTLFLFNDC LEIARKRHKVIGTFRSPHDRTRPPASLKHIHLMPLSQIKKVLDIRETEDCHNAFALLVRPPTEQANVLLS FQMTSEELPKESWLKMLCRHVANTICKADAENLMYVTDPESFEVNTKDMDSTLSRASRAIKKTSKKVTRA FSFSKTPKRALRMALSSSHSSEGRSPPSSGKLAVSRLSSTSSLAGIPSPSLVSLPSFFERRSHTLSRSTT HLI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001108547
ORF Size	2739 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001108547.2, NP_001102017.2
RefSeq Size	4043 bp
RefSeq ORF	2742 bp
Locus ID	361921
MW	103 kDa

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