Zmiz2 (NM_001100507) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1309256
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217326 representing NM_001100507 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCCCATGAACCCCATGAAACCTGCCCTGCCCCCTGCACCACACGGTGATGGTTCATTTGCATATG AGTCTGTGCCTTGGCAACAAAGTGCCACTCAGCCAGCTGGGTCACTGTCCGTGGTCACTACTGTGTGGGG AGTTGGCAACGCAACACAGAGCCAGGTTTTGGGGAACCCCATGGGCCCTGCAGGAAGCCCCCCTGGTGGC TCCATGATGCCTGGTGTGGCAGGTGGCAGCTCTGCCTTGACCTCCCCGCAGTGCCTGGGACAGCAGGCGT TTGCCGAAGGCGGTGCTAGCAAGGGCTACGTACAGCAAGGCGTGTATGGCCGAGGGGGCTACCCTGGGGG ATCCAGTTTCACTCCTGGGTATGCAGGAGGCCCTGGAGGCCTGGGGCTCCCCACACATGCAGCACGGCCC TCAACTGACTTCACACAAGCAGCAGCTGCAGCTGCCATGGCTGCTGCTGCAGCTACGGCCACAGCCACAG CCACAGCTACTGTGGCTGCCCTGCAAGAGAAGCAGAGCCAGGAGCTAAGCCAATATGGAGCGATGGGGAC TGGGCAATCTTTTAACAGCCAGTTTCTGCAGCATGGAGGTCCTCGAGGGCCCAGTGTACCCCCTGGCATG AACCCTTCAGGCATGGGAGGAATGATGGGCCCCTCAGGCCTTTCCTCCATGGCCATGAATCCTACACGGG CAGCAGGCATGACACCCTTGTATGCAGGACAGCGACTGCCTCAGCATGGCTACCCTGGGCCTCCCCAGGG GCAGCCGCTGCCTCGACAGGGGGTCAAGAGAGCCTACTCAGAGGTGTATCCTGGGCAGCAATATCTGCAA GGAGGCCAGTATGCAGCCAGCACTGCCCAGTATGCTCCTGGTCCCGGGCAGCCCCCTGGCCCTGCCTCCT CCTACGCAGGACACAGACTCCCCCTGCAGCAGGGCATGGCCCAGTCCCTGTCTGCCCCTGGCCCCACAGG ACTGCATTACAAGCCCACAGAGCAGTTCAACGGGCAGGGCGCCAGCTTCAACGGGGGCAGCATCAGCTAC AGCCAGCCTGGCTTGAGTGGGCCTTCCCGCTCCATCCCTGCTTACCCCAGCTCCCCACTGCCGGGGAATC CCACACCACCCATGACGCCTAGCAGCAGTGCTCCCTACATGTCCCCAAGCCAGGAGGTCAAGTCTCCTTT CCTGCCTGACCTCAAGCCAGGCCTCAGCTCCTTGCACCCATCACCCTCTGGAAGCGTCCCCTGTGACGAG CTGCGCCTGACCTTCCCAGTTCGGGATGGGGTGGTCCTGGAGCCCTTCCGCCTGCAGCACAACCTGGCTG TGAGCAACCATGTCTTCCAGCTCCGAGACTCTGTGTATAAAACCCTGATGCTGAGACCTGACTTAGAGCT GCAGTTCAAGTGCTACCACCACGAGGACCGGCAGATGAACACCAACTGGCCGGCCTCGGTGCAGGTCAGC GTCAATGCCACACCCCTCAGCATCGAGCGTGGAGACAACAAGACCTCGCACAAGCCCCTCTACCTGAAGC ATGTGTGCCAGCCGGGTCGCAACACCATCCAGATCACCGTCACCGCCTGCTGCTGCTCCCACCTCTTCGT GCTGCAGCTGGTGCACCGTCCGTCTGTCCGCTCTGTGCTGCAGGGTCTCCTCAAGAAGCGCCTCTTGCCA GCTGAGCACTGCATCACCAAGATAAAGCGGAACTTCAGTAGCGGCACCATCCCTGGCACCCCTGGGCCCA ATGGAGAGGATGGGGTGGAGCAGACGGCTATCAAGGTGTCCCTGAAGTGCCCCATCACCTTCCGCAGGAT CCAGCTCCCAGCTCGTGGTCACGACTGTCGCCACATACAGTGCTTTGACCTGGAGTCATACTTACAGCTT AACTGTGAGCGGGGGACCTGGAGGTGTCCGGTGTGCAACAAGACGGCATTGCTGGAGGGCCTGGAGGTGG ATCAGTATATGCTAGGCATCCTGATTTATATTCAGAACTCAGACTACGAGGAGATCACCATCGACCCCAC ATGCAGCTGGAAACCAGTGCCTGTGAAGCCTGACCTACACATCAAGGAGGAGCCGGACGGGCCAGTGCTG AAGCGCTGCCGTACTGTGAGCCCTGCCCACGTGCTCATGCCCAGTGTGATGGAGATGATTGCGGCCCTGG GCCCTGGCGCTGCCCCCTTTGCCCCATTGCAGCCCCCTTCGGCCCCTACCCCCAGCGACTACCCCAGCCA GGGTTCCAACTTCCTGGGGCCTGGAACCTTCCCAGAATCTTTCCCATCTGCTACACCCGCCACCCCAAAC CTTGCTGAGTTCACCCAGGGGCCACCCCCAATCTCCTACCAGTCTGACATTCCCAGCAGCCTCCTGACTC CAGACAAGTCTGCTCCTTGCCTCCCAGGCCAGATGGCACCAGCAGGTCACCTGGACCCAGCCCATAATCC TGGACCACCAGGACTGCACACTCCCAACCTCGGGCCCACCCCAGGCACCCAGCTACACCATCCAAACCCT TCCCCTGCATCCAGGCAGCCCCTGGGCCAACCAAACACAGGGCCCATCAGTGAACTGGCTTTCAATCCTG CCACGGGCATGATGGGGCCTCCCAGCATGACTGGGGCAGGGGAGGCCTCAGAACCAGCTCTGGATCTGCT CCCAGAACTGACCAATCCTGATGAACTACTCTCCTACCTGGGTCCACCTGACCTCCCCACAAACAGCAGT GATGATCTGCTCTCACTCTTTGAGAACAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217326 representing NM_001100507 Red=Cloning site Green=Tags(s) MNPMNPMKPALPPAPHGDGSFAYESVPWQQSATQPAGSLSVVTTVWGVGNATQSQVLGNPMGPAGSPPGG SMMPGVAGGSSALTSPQCLGQQAFAEGGASKGYVQQGVYGRGGYPGGSSFTPGYAGGPGGLGLPTHAARP STDFTQAAAAAAMAAAAATATATATATVAALQEKQSQELSQYGAMGTGQSFNSQFLQHGGPRGPSVPPGM NPSGMGGMMGPSGLSSMAMNPTRAAGMTPLYAGQRLPQHGYPGPPQGQPLPRQGVKRAYSEVYPGQQYLQ GGQYAASTAQYAPGPGQPPGPASSYAGHRLPLQQGMAQSLSAPGPTGLHYKPTEQFNGQGASFNGGSISY SQPGLSGPSRSIPAYPSSPLPGNPTPPMTPSSSAPYMSPSQEVKSPFLPDLKPGLSSLHPSPSGSVPCDE LRLTFPVRDGVVLEPFRLQHNLAVSNHVFQLRDSVYKTLMLRPDLELQFKCYHHEDRQMNTNWPASVQVS VNATPLSIERGDNKTSHKPLYLKHVCQPGRNTIQITVTACCCSHLFVLQLVHRPSVRSVLQGLLKKRLLP AEHCITKIKRNFSSGTIPGTPGPNGEDGVEQTAIKVSLKCPITFRRIQLPARGHDCRHIQCFDLESYLQL NCERGTWRCPVCNKTALLEGLEVDQYMLGILIYIQNSDYEEITIDPTCSWKPVPVKPDLHIKEEPDGPVL KRCRTVSPAHVLMPSVMEMIAALGPGAAPFAPLQPPSAPTPSDYPSQGSNFLGPGTFPESFPSATPATPN LAEFTQGPPPISYQSDIPSSLLTPDKSAPCLPGQMAPAGHLDPAHNPGPPGLHTPNLGPTPGTQLHHPNP SPASRQPLGQPNTGPISELAFNPATGMMGPPSMTGAGEASEPALDLLPELTNPDELLSYLGPPDLPTNSS DDLLSLFENN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001100507
ORF Size	2760 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001100507.1, NP_001093977.1
RefSeq Size	4385 bp
RefSeq ORF	2763 bp
Locus ID	289783
MW	96.5 kDa

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