Adam19 (NM_001160228) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Sox30
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217327 representing NM_001160228 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCGGGCGCGCGGGCGTCGCCCGGTTCTGCTTGCTGGCTCTCGCTCTGCAGCTACAGTGGCCGCTGG CGGCATGCGAACCGGAATGGACCACAAGAGGAAGCAAAGAAGACAGCCCTCCACTACAACATGAACTCAT AATACCTCAGTGGAGGACTTCAGAAAGCCCCAGGGGAGGAAAGCATCCACTCACGGCAGAGCTCAGGGTC ATGGCTGAAGGGCGAGAGCTGATCCTGGACCTGGAGAAGAACGAGCACCTTTTTGCTCCAGCCTACACAG AGACCTGCTACACCGCAAGTGGCAATCCTCAGACCACCACGCTGAAATCTGAGGATCACTGCTTTTACCA CGGGACTGTGAGGAAAGTGGATGAGTCCAGCGTCACTCTCAGCACCTGCCGGGGAATTAGAGGACTGATT ATAGTGAGAAGTAACCTCAGCTACATCATCGAGCCCGTCCCTAACAGCGACAACCAACACCGTATTTACA GATCCGAACATCTCAAGCTGCCCCCGGGGAACTGTGGGTTCGAGCACTCCAGGCCCACCTCTAAGGACTG GACCCTTCAGTTTACACATCAGACCAAAAAGCAACCTCGCAGAATGAAACGGGAAGATTTACACTCTATG AAGTACGTGGAGCTTTATCTGGTGGCTGATTACGCAGAGTTTCAGAAGAACCGGCATGACCAGGATGCCA CCAAACACAAGCTCATGGAGATTGCCAACTATGTTGATAAGTTTTACCGCTCCCTGAGCATCCGGATTGC GCTTGTCGGCTTGGAGGTGTGGACACACGGGGATAAGTGTGAAGTCTCAGAGAATCCGTACTCTACCCTC TGGTCCTTTCTCAGTTGGAGGCGCAAGCTGCTCACCCAGAAGAGCCATGACAATGCCCAGCTAATCACGG GCAGGTCCTTCCAAGGCACCACCATTGGCCTGGCCCCCCTCATGGCCATGTGTTCTGTGTACCAGTCCGG AGGAGTTAGCATGGACCACTCCGAGAATGCCATCGGTGTAGCCTCCACCGTGGCCCACGAGATTGGCCAC AACTTTGGCATGAGCCATGATTCTGCACACTGCTGTTCTACCAGCGCAGCCGATGGTGGGTGCATCATGG CTGCTGCTACGGGGCACCCTTTCCCCAAAGTGTTCAGTTCGTGCAACAGGAAGGAGCTGGACAGGTATCT GCAGTCAGGAGGTGGGATGTGTCTCTCCAACATGCCAGATACGAGGACTCTGTATGGAGGCCGGAGGTGT GGCAATGGGTACCTGGAAGACGGTGAAGAATGTGACTGCGGAGAAGAGGACGAATGTAAGAACCCTTGCT GCAATGCCTCCAACTGCACTCTGAAGGAAGGGGCCGAGTGTGCCCATGGCTCCTGCTGCCACCAGTGTAA GCTGGTGGCTCCTGGAACCCAGTGTCGTGAGCAGGTGCGGCAGTGTGATCTCCCCGAGTTCTGCACGGGC AAGTCCCCCCACTGCCCCACCAACTATTACCAGATGGATGGCACCCCCTGCGAGGGTGGCCAGGCCTACT GCTACAATGGCATGTGCCTCACTTATCAGGAGCAGTGCCAGCAGCTGTGGGGACCTGGAGCCAGGCCTGC TCTTGATCTTTGCTTCGAGAGGGTGAATGCTGCTGGTGACACCTATGGTAACTGCGGCAAAGGCTTGAAT GGCAAATACAGGAAGTGCAGCCCCAGGGATGCCAAGTGTGGGAAGATCCAGTGTCAGAGCACCCAGGCCA GGCCCCTGGAATCCAACGCAGTATCTATTGACACCACCATTACCTTGAACGGGAGGCGGATCCACTGTCG GGGCACCCATGTCTACCGGGGTCCTGAGGAGGAAGAAGGGGAGGGTGACATGCTGGACCCAGGGCTGGTG ATGACCGGGACCAAGTGTGGCCACAACCATATTTGCTTCGAGGGGCAATGCAGGAACACCTCCTTCTTTG AGACGGAAGGCTGTGGGAAAAAGTGCAATGGTCATGGGGTCTGCAACAACAACAAGAACTGTCATTGCTT CCCTGGCTGGGCTCCACCTTTCTGTAACACCCCAGGAGATGGCGGCAGCATTGACAGTGGGCCTTTGCCC CCTAAGAGTGCGGGTCCTGTGATTGCTGGGGTGTTTTCTGCCCTCTTCGTGTTGGCAGTCCTGGTGCTAT TGTGTCATTGCTACAGACAGAGCCACAAACTGGGCAAGCCCTCAGCTCTCCCTTTTAAGCTGCGGCAACA GTTCAGTTGTCCCTTCAGGGTGTCTCAGAATGGTGGAACTGGCCACGCCAACCCAACTTTCAAGTTGCAG ACACCCCAGGGCAAGCGAAAGGTGACTAACACCCCTGAAACTCTCCGGAAGCCCTCCCACCCTCCTCCCC GGCCCCCTCCGGACTACCTACGAGTTGAATCCCATCCTGCACCACTGCCAGCACATCTGAACAGGGCTGC TGGGAGCTCACCAGAAGCTGGAGCTCAGATAGAAAGAAAGGAATCAGCCAGAAGACCTCCCCCAAGCCGG CCCATGCCCCCTGCACCGAACTGCCTACTGTCCCAGGATTTCTCCAGGCCTCGACCACCTCAGAAGGCAC TCCCAGCGAATCCTGTGCCGGGCCAAAGGACTGTTCCCAGGCCAGGAGGCACCTCCCTGTTTCATCCCCC TACTACTGGTCCTCAGCCTCCCAGGCCTCCAGCAGTGTCTGTTCCAAAGCTTCCTGAGTACCGATCACAG AGGATTGGAGGGATAACCAGCTCCAAGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217327 representing NM_001160228 Red=Cloning site Green=Tags(s) MPGRAGVARFCLLALALQLQWPLAACEPEWTTRGSKEDSPPLQHELIIPQWRTSESPRGGKHPLTAELRV MAEGRELILDLEKNEHLFAPAYTETCYTASGNPQTTTLKSEDHCFYHGTVRKVDESSVTLSTCRGIRGLI IVRSNLSYIIEPVPNSDNQHRIYRSEHLKLPPGNCGFEHSRPTSKDWTLQFTHQTKKQPRRMKREDLHSM KYVELYLVADYAEFQKNRHDQDATKHKLMEIANYVDKFYRSLSIRIALVGLEVWTHGDKCEVSENPYSTL WSFLSWRRKLLTQKSHDNAQLITGRSFQGTTIGLAPLMAMCSVYQSGGVSMDHSENAIGVASTVAHEIGH NFGMSHDSAHCCSTSAADGGCIMAAATGHPFPKVFSSCNRKELDRYLQSGGGMCLSNMPDTRTLYGGRRC GNGYLEDGEECDCGEEDECKNPCCNASNCTLKEGAECAHGSCCHQCKLVAPGTQCREQVRQCDLPEFCTG KSPHCPTNYYQMDGTPCEGGQAYCYNGMCLTYQEQCQQLWGPGARPALDLCFERVNAAGDTYGNCGKGLN GKYRKCSPRDAKCGKIQCQSTQARPLESNAVSIDTTITLNGRRIHCRGTHVYRGPEEEEGEGDMLDPGLV MTGTKCGHNHICFEGQCRNTSFFETEGCGKKCNGHGVCNNNKNCHCFPGWAPPFCNTPGDGGSIDSGPLP PKSAGPVIAGVFSALFVLAVLVLLCHCYRQSHKLGKPSALPFKLRQQFSCPFRVSQNGGTGHANPTFKLQ TPQGKRKVTNTPETLRKPSHPPPRPPPDYLRVESHPAPLPAHLNRAAGSSPEAGAQIERKESARRPPPSR PMPPAPNCLLSQDFSRPRPPQKALPANPVPGQRTVPRPGGTSLFHPPTTGPQPPRPPAVSVPKLPEYRSQ RIGGITSSKI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001160228
ORF Size	2760 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001160228.1, NP_001153700.1
RefSeq Size	6286 bp
RefSeq ORF	2763 bp
Locus ID	303068
MW	101.1 kDa
Gene Summary	membrane-bound metalloprotease-disintegrin similar to meltrin beta; may be involved in neural crest, muscle and skeletal developmental morphogenesis [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.