Adam19 (NM_001160228) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Sox30
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217327 representing NM_001160228 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCGGGCGCGCGGGCGTCGCCCGGTTCTGCTTGCTGGCTCTCGCTCTGCAGCTACAGTGGCCGCTGG CGGCATGCGAACCGGAATGGACCACAAGAGGAAGCAAAGAAGACAGCCCTCCACTACAACATGAACTCAT AATACCTCAGTGGAGGACTTCAGAAAGCCCCAGGGGAGGAAAGCATCCACTCACGGCAGAGCTCAGGGTC ATGGCTGAAGGGCGAGAGCTGATCCTGGACCTGGAGAAGAACGAGCACCTTTTTGCTCCAGCCTACACAG AGACCTGCTACACCGCAAGTGGCAATCCTCAGACCACCACGCTGAAATCTGAGGATCACTGCTTTTACCA CGGGACTGTGAGGAAAGTGGATGAGTCCAGCGTCACTCTCAGCACCTGCCGGGGAATTAGAGGACTGATT ATAGTGAGAAGTAACCTCAGCTACATCATCGAGCCCGTCCCTAACAGCGACAACCAACACCGTATTTACA GATCCGAACATCTCAAGCTGCCCCCGGGGAACTGTGGGTTCGAGCACTCCAGGCCCACCTCTAAGGACTG GACCCTTCAGTTTACACATCAGACCAAAAAGCAACCTCGCAGAATGAAACGGGAAGATTTACACTCTATG AAGTACGTGGAGCTTTATCTGGTGGCTGATTACGCAGAGTTTCAGAAGAACCGGCATGACCAGGATGCCA CCAAACACAAGCTCATGGAGATTGCCAACTATGTTGATAAGTTTTACCGCTCCCTGAGCATCCGGATTGC GCTTGTCGGCTTGGAGGTGTGGACACACGGGGATAAGTGTGAAGTCTCAGAGAATCCGTACTCTACCCTC TGGTCCTTTCTCAGTTGGAGGCGCAAGCTGCTCACCCAGAAGAGCCATGACAATGCCCAGCTAATCACGG GCAGGTCCTTCCAAGGCACCACCATTGGCCTGGCCCCCCTCATGGCCATGTGTTCTGTGTACCAGTCCGG AGGAGTTAGCATGGACCACTCCGAGAATGCCATCGGTGTAGCCTCCACCGTGGCCCACGAGATTGGCCAC AACTTTGGCATGAGCCATGATTCTGCACACTGCTGTTCTACCAGCGCAGCCGATGGTGGGTGCATCATGG CTGCTGCTACGGGGCACCCTTTCCCCAAAGTGTTCAGTTCGTGCAACAGGAAGGAGCTGGACAGGTATCT GCAGTCAGGAGGTGGGATGTGTCTCTCCAACATGCCAGATACGAGGACTCTGTATGGAGGCCGGAGGTGT GGCAATGGGTACCTGGAAGACGGTGAAGAATGTGACTGCGGAGAAGAGGACGAATGTAAGAACCCTTGCT GCAATGCCTCCAACTGCACTCTGAAGGAAGGGGCCGAGTGTGCCCATGGCTCCTGCTGCCACCAGTGTAA GCTGGTGGCTCCTGGAACCCAGTGTCGTGAGCAGGTGCGGCAGTGTGATCTCCCCGAGTTCTGCACGGGC AAGTCCCCCCACTGCCCCACCAACTATTACCAGATGGATGGCACCCCCTGCGAGGGTGGCCAGGCCTACT GCTACAATGGCATGTGCCTCACTTATCAGGAGCAGTGCCAGCAGCTGTGGGGACCTGGAGCCAGGCCTGC TCTTGATCTTTGCTTCGAGAGGGTGAATGCTGCTGGTGACACCTATGGTAACTGCGGCAAAGGCTTGAAT GGCAAATACAGGAAGTGCAGCCCCAGGGATGCCAAGTGTGGGAAGATCCAGTGTCAGAGCACCCAGGCCA GGCCCCTGGAATCCAACGCAGTATCTATTGACACCACCATTACCTTGAACGGGAGGCGGATCCACTGTCG GGGCACCCATGTCTACCGGGGTCCTGAGGAGGAAGAAGGGGAGGGTGACATGCTGGACCCAGGGCTGGTG ATGACCGGGACCAAGTGTGGCCACAACCATATTTGCTTCGAGGGGCAATGCAGGAACACCTCCTTCTTTG AGACGGAAGGCTGTGGGAAAAAGTGCAATGGTCATGGGGTCTGCAACAACAACAAGAACTGTCATTGCTT CCCTGGCTGGGCTCCACCTTTCTGTAACACCCCAGGAGATGGCGGCAGCATTGACAGTGGGCCTTTGCCC CCTAAGAGTGCGGGTCCTGTGATTGCTGGGGTGTTTTCTGCCCTCTTCGTGTTGGCAGTCCTGGTGCTAT TGTGTCATTGCTACAGACAGAGCCACAAACTGGGCAAGCCCTCAGCTCTCCCTTTTAAGCTGCGGCAACA GTTCAGTTGTCCCTTCAGGGTGTCTCAGAATGGTGGAACTGGCCACGCCAACCCAACTTTCAAGTTGCAG ACACCCCAGGGCAAGCGAAAGGTGACTAACACCCCTGAAACTCTCCGGAAGCCCTCCCACCCTCCTCCCC GGCCCCCTCCGGACTACCTACGAGTTGAATCCCATCCTGCACCACTGCCAGCACATCTGAACAGGGCTGC TGGGAGCTCACCAGAAGCTGGAGCTCAGATAGAAAGAAAGGAATCAGCCAGAAGACCTCCCCCAAGCCGG CCCATGCCCCCTGCACCGAACTGCCTACTGTCCCAGGATTTCTCCAGGCCTCGACCACCTCAGAAGGCAC TCCCAGCGAATCCTGTGCCGGGCCAAAGGACTGTTCCCAGGCCAGGAGGCACCTCCCTGTTTCATCCCCC TACTACTGGTCCTCAGCCTCCCAGGCCTCCAGCAGTGTCTGTTCCAAAGCTTCCTGAGTACCGATCACAG AGGATTGGAGGGATAACCAGCTCCAAGATCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001160228
Insert Size	2763 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001160228.1, NP_001153700.1
RefSeq Size	6286 bp
RefSeq ORF	2763 bp
Locus ID	303068
Gene Summary	membrane-bound metalloprotease-disintegrin similar to meltrin beta; may be involved in neural crest, muscle and skeletal developmental morphogenesis [RGD, Feb 2006]

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