Ccar2 (NM_001170472) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1309922
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217330 representing NM_001170472 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCAGTTTAAGCGCCAGAGGATCAACCCACTTCCAGGGGGACGGAACTTCTCAGGCGCAGCTTCAA CATCTCTTTTGGGTCCTCCTCCTGGTCTGCTTACTCCTCCTGTGGCCACAGACTTGTCCCAAAATGCCAG GCACCTTCAGAGTGGGGAGAAGCAGCGAGTCTTCACTGGCATTGTTACCAGCTTGCATGACTACTTTGGG GTGGTAGATGAAGAAGTCTTTTTTCAGCTAAGTGTGGTGAAGGGCCGACTACCCCAGCTGGGTGAGAAGG TGCTGGTGAAGGCTGCGTATAACCCAGGCCAGGCAGTGCCCTGGAATGCTGTCAAGGTGCAGACGCTCTC CAACCAGCCCTTACTGAAGTCTCCAGCACCTCCCCTTCTACATGTGGCAGCTCTGGGCCAGAAGCAAGGG ATCCTCGGAGCTCAGCCCCAGTTGATCTTTCAGCCTCACCGAATTCCCCCACTTTTCCCTCAGAAGCCTC TGAGTCTCTTCCAGACATCCCATACACTTCACCTGAGCCACCTGAACAGATTTCCTGCTCGGGGTCCTCA TGGAAGGCTGGACCAGGGCCGAAGTGACGACTATGACTCGAAGAAACGCAAACAGCGGGCTGGTGGAGAG CCTTGGGGTGCTAAGAAACCTAGGCATGACCTGCCTCCTTACCGGGTGCATCTCACTCCCTATGCTGTGG ACAGTCCCACCTGTGACTTCCTAGAACTGCAGCGACGTTACCGCAGCCTGTTGGTCCCCTCAGATTTTCT TTCCGTTCACTTGAGCTGGCTGTCAGCCTTCCCACTGGGCCAGCCCTTTTCCCTCCACCACCCGAGTCGG ATCCAGGTCTCTTCAGAGAAGGAGGCAGCTCCAGACACTGGTGCTGAGCCCAGCCCTGAGGACAGTGACC CCACTTACAGTTCCAAGGTGCTGCTGCTCTCCTCCCCAGGCCTAGAGGAGTTCTATCGTTGCTGCATGCT GTTTGTGGATGACATGGCTGAGCCAAGGGAGACACCAGAGCATCCTCTGAAGCAGCTAAAGTTTTTGCTG GGCCGAAAAGAAGAAGAGGCAGTGCTGGTTGGGGGTGAGTGGTCTCCTTCCCTGGATGGCCTCGACCCCC AGGCAGACCCGCAGGTGCTGGTGCGCACAGCCATCCGCTGTGCACAAGCCCAGACTGGCATTGACTTGAG CACCTGTACCAAATGGTGGCGTTTTGCTGAGTTTCAGTATTTGCAGCCGGGGCCGCCCCGGCAGTTACAC ACAGTAGTGGTGTACCTGCCAGATGTGTGGACCATCATGCCCACTTTGGAGGAGTGGGAGGCACTGTGCC AACAGAAAGCCACAGAAGCAGCTCCCCAACCGCAGGAGGCATCAGGGGAGGCAGAGGCTACTGAACAGGC TCCTGACGTGTCAGAGCAAGCAGACACTTCTAAACAGGACACAGAGACAGCAGAGGCCACCACACAGCAA GATGTGGACACTGAGCTCCCAGAGGCCCCTCCGCCTCCTCTAGAACCTGCGGTGATGGCACGTCCTGGCT GTGTCAACCTGTCTCTCTACGGAATTGTGGAGGATCGGAGGCCAAAAGAAAGGATCTCTTTTGAGGTGGT GGTGCTGGCCGAGCTGTTTGTGGAGATGCTGCAGAGGGACTTTGGCTATAGAATTTATAAGGTGCTGCTG AGCCTTCCAGAAAAAGTTGTGTCTCCTCCTGAACCTGAGAAGGAGGAGGCAGCCAAGGAGGATGTGGTCA AAGAGGAGGAGGCTGTCAAAGAGGAGGCAGTGAAGGTGTCCAAGGATGAGGTACAGAATGAGGGCACAGC TGCCGAGGCAGACAGCCCACTGAAGGAGGATGGGCTTCTGCCCAAACAGCCCTCTGCAGGGGGAGAAGAG GAGGAGAAGGCCCGGGGTGAGGCAGCTGAGGACCTCTGTGAAATGGCCTTGGACCCAGACCTGCTGCTTC TGAGGGATGATGGAGAGGAAGAGTTTGCAGGAGCAAAGCTAGAGGAAACGGAGGTTCGCTCTGTTGCCTC CAACCAGTCCGAGATGGAATATTCTTCTCTTCAGGACATGCCTAAGGAGCTGGATCCCTCAGCTGTGCTC CCCTTGGACTGCCTTCTGGCTTTTGTGTTTTTTGATGCCAACTGGTGTGGCTACTTGCACAGGCGAGACT TGGAGAGGATCCTCCTCACCTTGGGGATCCGGCTCAGTGCAGAGCAGGCCAAACAGCTGGTTAGTAGAGT GGTGGCACAGAACATCTGCCAGTATCGGAGCCTTCAGTATAGCCGTGCTGAGGCGCTGGATGACGGGCTT CCAGAAGACGTGCTCTTTGGAAACCTGGATCTACTGCCTCCTTCAGGAAAGAGCACAAAGCCAGGTGCTG CCCCCACAGAGCACAAAGGCCTGGTGCCCCACAATGGTAGCCTCATCAATGTGGGAAGCCTGTTACAGCG TGCAGAGCAGCAAGACAGTGGCCGCCTGTACCTGGAGAACAAGATTCACACACTGGAATTGAAGCTTGAG GAGAGCCATAACCGATTTTCAGCCACTGAAGTGACAAATAAGACACTGGCAGCAGAGATGCAGGAGCTGC GGGCTCGGCTGGCCGAGGCGGAGGAGACAGCCCGGACAGTGGAACGACAGAAGAACCAGCTCCAGCGTCA GATGCAGGACTTCCGCAGACGCCTGACCCCACTGCACCTTGAGATGCAGCGGATCGTTGAAAAGGCTGAC AGCTGGGTAGAGAAAGAGGAGCCAACGCCTAGCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217330 representing NM_001170472 Red=Cloning site Green=Tags(s) MSQFKRQRINPLPGGRNFSGAASTSLLGPPPGLLTPPVATDLSQNARHLQSGEKQRVFTGIVTSLHDYFG VVDEEVFFQLSVVKGRLPQLGEKVLVKAAYNPGQAVPWNAVKVQTLSNQPLLKSPAPPLLHVAALGQKQG ILGAQPQLIFQPHRIPPLFPQKPLSLFQTSHTLHLSHLNRFPARGPHGRLDQGRSDDYDSKKRKQRAGGE PWGAKKPRHDLPPYRVHLTPYAVDSPTCDFLELQRRYRSLLVPSDFLSVHLSWLSAFPLGQPFSLHHPSR IQVSSEKEAAPDTGAEPSPEDSDPTYSSKVLLLSSPGLEEFYRCCMLFVDDMAEPRETPEHPLKQLKFLL GRKEEEAVLVGGEWSPSLDGLDPQADPQVLVRTAIRCAQAQTGIDLSTCTKWWRFAEFQYLQPGPPRQLH TVVVYLPDVWTIMPTLEEWEALCQQKATEAAPQPQEASGEAEATEQAPDVSEQADTSKQDTETAEATTQQ DVDTELPEAPPPPLEPAVMARPGCVNLSLYGIVEDRRPKERISFEVVVLAELFVEMLQRDFGYRIYKVLL SLPEKVVSPPEPEKEEAAKEDVVKEEEAVKEEAVKVSKDEVQNEGTAAEADSPLKEDGLLPKQPSAGGEE EEKARGEAAEDLCEMALDPDLLLLRDDGEEEFAGAKLEETEVRSVASNQSEMEYSSLQDMPKELDPSAVL PLDCLLAFVFFDANWCGYLHRRDLERILLTLGIRLSAEQAKQLVSRVVAQNICQYRSLQYSRAEALDDGL PEDVLFGNLDLLPPSGKSTKPGAAPTEHKGLVPHNGSLINVGSLLQRAEQQDSGRLYLENKIHTLELKLE ESHNRFSATEVTNKTLAAEMQELRARLAEAEETARTVERQKNQLQRQMQDFRRRLTPLHLEMQRIVEKAD SWVEKEEPTPSN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001170472
ORF Size	2766 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001170472.1, NP_001163943.1
RefSeq Size	3613 bp
RefSeq ORF	2769 bp
Locus ID	306007
MW	102.8 kDa

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