Unc5d (NM_001107319) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217360 representing NM_001107319 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGACAGGGGCTGCAGACCGCAGCCGCGGGGCGCGCTGGTGGCTCCCATGGCTTGGACTCTGTTTCT GGGCGGCGGGGGCTGAGGCTGCCCGAGGAGCCGACAGTGGCGAAGTTCTTCCCGACTCCATCCCGTCTGC CCCTGGAACTCTGCCCCATTTCATAGAGGAGCCTGAAGATGCCTACATCATCAAAAGCAACCCTATCGCA CTCAGGTGCAAAGCCAGGCCCGCCATGCAGATCTTCTTCAAATGCAACGGGGAGTGGGTCCATCAGAACG AGCACGTGTCAGAAGAAAGCCTGGATGAGAGCTCAGGCTTGAAGGTCCGGGAGGTGTTTATCAATGTCAC CAGGCAGCAGGTGGAGGACTTCCATGGGCCCGAGGACTACTGGTGCCAGTGTGTTGCGTGGAGCCACCTT GGAACTTCCAAGAGTAGGAAAGCTTCCGTGCGCATAGCCTATTTACGGAAAAACTTCGAGCAAGATCCAC AAGGCAGGGAGGTTCCTATTGAAGGCATGATCGTATTGCACTGCCGCCCGCCAGAGGGAGTTCCAGCAGC AGAGGTGGAATGGCTGAAAAATGAAGAGCCCATTGACTCTGAACAGGATGAGAACATTGACACCAGGGCT GACCATAACCTGATCATCAGGCAGGCCCGGCTCTCAGACTCGGGGAATTACACCTGTATGGCAGCCAACA TCGTGGCCAAGAGGAGGAGCCTGTCAGCAACTGTGGTGGTCTACGTGAATGGAGGCTGGTCTTCCTGGAC AGAGTGGTCAGCCTGCAATGTTCGCTGTGGTAGAGGATGGCAGAAACGTTCCCGGACCTGCACCAACCCC GCTCCTCTCAATGGTGGGGCCTTTTGTGAGGGAATGTCAGTGCAGAAAATAACCTGCACTGCTCTTTGTC CTGTGGACGGCAGCTGGGAAGTATGGAGTGAATGGTCAGTCTGCAGCCCAGAGTGTGAACATCTTCGCAT CCGTGAGTGCACAGCTCCACCCCCAAGAAACGGGGGCAAGTTCTGTGAAGGGCTCAGCCAGGAATCTGAA AACTGCACGGATGGTCTCTGCATTCTAGATAAAAAACCTCTTCATGAAATAAAACCCCAAAGATGGAGTA GGCGAGGCATTGAGAATGCCAGCGACATTGCTTTGTACTCGGGCTTGGGTGCAGCCGTTGTAGCCGTTGC GGTCCTGGTCATTGGTGTCACCCTCTACAGACGGAGCCATAGTGACTACGGAGTGGACGTCATTGACTCT TCTGCATTGACAGGTGGCTTCCAGACCTTCAACTTCAAAACAGTTCGTCAAGGAAATTCTCTCCTCCTGA ATCCTGCCATGCATCCAGATCTGACTGTGAGCCGGACGTACAGCGGGCCCATCTGTCTGCAGGATCCACT GGACAAGGAGCTCATGACAGAGTCCTCACTCTTCAACCCTCTCTCTGACATCAAAGTCAAAGTCCAGAGC TCATTCATGGTTTCCCTGGGAGTGTCTGAGAGAGCCGAGTACCATGGCAAGAATCACTCCGGGACCTTCC CCCATGGAAACAACCGTGGATTTAGTACCATACATCCCAGAAACAAGACTCCGTACATCCAAAATCTGTC GTCACTGCCCACAAGGACGGAGCTGAGGACAACTGGTGTCTTTGGACACTTAGGGGGGCGCTTAGTAATG CCAAATACAGGGGTGAGTCTACTCATACCACATGGTGCCATCCCAGAGGAGAATTCTTGGGAGATTTATA TGTCAATCAACCAAGGTGAACCCAGTCTGCAGTCAGATGGATCTGAGGTCCTCCTGAGTCCTGAAGTCAC CTGTGGGCCTCCAGATATGCTTGTCACAACTCCCTTTGCACTGACCATCCCTCACTGTGCAGACGTCAGT TCAGAGCATTGGAACATCCACTTGAAGAAGAGAACGCAGCAGGGAAAGTGGGAGGAAGTAATGTCAGTGG AGGATGAGTCCACATCCTGTTACTGCCTCTTGGATCCCTTCGCCTGTCATGTGCTGCTTGATAGCTTTGG AACGTATGCCCTCACGGGAGAGCCCATCACAGACTGTGCGGTAAAGCAGCTCAAGGTGGCTGTGTTTGGC TGCATGTCCTGTAACTCCTTGGATTACAACCTGAGAGTTTATTGTGTGGACAACACTCCTTGTGCATTTC AGGAAGTGGTTTCTGATGAACGGCACCAAGGCGGCCAGCTCCTGGAGGAGCCAAAGCTGCTTCATTTCAA AGGGAATACATTCAGTCTTCAGATCTCTGTCCTTGACATCCCTCCATTCCTCTGGAGGATCAAACCATTC ACTGCATGCCAGGAAGTCCCCTTCTCCAGAGTGTGGAGCAGCAACCGGCAGCCCCTGCATTGTGCCTTCT CCCTGGAGCGCTACACGCCCACCACCACCCAGCTGTCCTGCAAAATCTGCATTCGGCAGCTCAAAGGCCA TGAACAGATCCTCCAAGTGCAGACATCCATCCTAGAGAGTGAACGAGAAACCATCACTTTCTTCGCACAA GAGGACAGTACTTTCCCTGCACAGACTGGCCCGAAAGCCTTCAAAATTCCCTATTCCATCAGGCAGAGGA TCTGCGCAACGTTCGATACCCCCAATGCCAAAGGCAAGGACTGGCAGATGTTAGCACAGAAAAACAGCAT CAATAGGAATTTATCCTATTTTGCTACACAAAGCAGTCCCTCTGCTGTCATTTTGAACCTCTGGGAAGCT CGTCATCAACAGGACGGGGATCTTGACTCGCTCGCCTGTGCCCTTGAAGAGATTGGGAGGACACACACGA AACTATCAAACATCACAGAGCCCCAGCTCGATGACACTGACTTCAACTACAGCAGGCAAAATGGACTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217360 representing NM_001107319 Red=Cloning site Green=Tags(s) MGTGAADRSRGARWWLPWLGLCFWAAGAEAARGADSGEVLPDSIPSAPGTLPHFIEEPEDAYIIKSNPIA LRCKARPAMQIFFKCNGEWVHQNEHVSEESLDESSGLKVREVFINVTRQQVEDFHGPEDYWCQCVAWSHL GTSKSRKASVRIAYLRKNFEQDPQGREVPIEGMIVLHCRPPEGVPAAEVEWLKNEEPIDSEQDENIDTRA DHNLIIRQARLSDSGNYTCMAANIVAKRRSLSATVVVYVNGGWSSWTEWSACNVRCGRGWQKRSRTCTNP APLNGGAFCEGMSVQKITCTALCPVDGSWEVWSEWSVCSPECEHLRIRECTAPPPRNGGKFCEGLSQESE NCTDGLCILDKKPLHEIKPQRWSRRGIENASDIALYSGLGAAVVAVAVLVIGVTLYRRSHSDYGVDVIDS SALTGGFQTFNFKTVRQGNSLLLNPAMHPDLTVSRTYSGPICLQDPLDKELMTESSLFNPLSDIKVKVQS SFMVSLGVSERAEYHGKNHSGTFPHGNNRGFSTIHPRNKTPYIQNLSSLPTRTELRTTGVFGHLGGRLVM PNTGVSLLIPHGAIPEENSWEIYMSINQGEPSLQSDGSEVLLSPEVTCGPPDMLVTTPFALTIPHCADVS SEHWNIHLKKRTQQGKWEEVMSVEDESTSCYCLLDPFACHVLLDSFGTYALTGEPITDCAVKQLKVAVFG CMSCNSLDYNLRVYCVDNTPCAFQEVVSDERHQGGQLLEEPKLLHFKGNTFSLQISVLDIPPFLWRIKPF TACQEVPFSRVWSSNRQPLHCAFSLERYTPTTTQLSCKICIRQLKGHEQILQVQTSILESERETITFFAQ EDSTFPAQTGPKAFKIPYSIRQRICATFDTPNAKGKDWQMLAQKNSINRNLSYFATQSSPSAVILNLWEA RHQQDGDLDSLACALEEIGRTHTKLSNITEPQLDDTDFNYSRQNGL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107319
ORF Size	2868 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107319.2, NP_001100789.2
RefSeq Size	3057 bp
RefSeq ORF	2871 bp
Locus ID	306534
UniProt ID	F1LW30
MW	106.5 kDa
Gene Summary	Receptor for the netrin NTN4 that promotes neuronal cell survival. Plays a role in cell-cell adhesion and cell guidance. Receptor for netrin involved in cell migration. Plays a role in axon guidance by mediating axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. May play a role in apoptosis in response to DNA damage. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand (By similarity). Mediates cell-cell adhesion via its interaction with FLRT3 on an adjacent cell (By similarity).[UniProtKB/Swiss-Prot Function]

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