Nebl (NM_001191694) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Lasp-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217412 representing NM_001191694 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGTGCCTGTATCCGGGGATGTGAAAGAGGAAACCGAAGAGGAAAATGTGGAGGAGGAAGAAAAGC CAGAAGACGAGGTGTTTCTGAAACCTGTCGTTGAAGACCTAAGCATGGAGCTGGCCAGAAAGTGCACCGA GCTCATTAGTGACATCCATTATAAAGAAGACTATAGAAAGTCTAAGGACAAGTGTACGTCTGTGACTGAT ACCCCTACGCTAAACCATGTGAAAAATATTAGTGCTTTTATTTCTGAGACAAAATACAAAGGTACCATTA AAGCTGATCTTTCTAACTGTCTGTATAAGGACATGCCTGCTACCATTGACAGTGTCTTTGCAAGAGAAGT GTCCCAGCTTCAGAGTGAGGTTGCCTATAAGCAGAAGCATGAGGCGGAGAAAGGCCTCTCCGATTATGCC CACATGAAGGAACCACCGGAGGTCAGACACGCCATGGAGGTCAACAGGCACCAGAGTAACATTTCTTACA GAAAAGATGTGCAAGGCACCCACACGTATACAGCGGAGATGGACAGACCGGATATCAAGAAGGCAACTCA GATCTCCAAGATCATAAGCAATGCAGAATACAAGAAAGGACAAGGCATCGTGAATAAAGAACCTTCTGTA ATTGGGAGACCAGATTTTGAACATGCCGTGGAAGCTTCTAAACTTTCTAGTCAGGTTAAATATAAAGAAA AGTTTGATAATGAAATGAAGGGAAAAGGCCACCATTACAACCCTCTGGGAAGTGCGTCTTTCAGACAACA CCAGCTCGCTACTGTCCTGGCAAGCGATGTGAAGTACAAGAAAGATGTTCAAACCATGCACGAGCCAGTT TCAGATCTCCCAAACTTGTTGTTCTTAGACCATGCTCTGAAAGCCAGCAGAATGCTCAGTGGATGGGAAT ATAAAAAGAATTTTGAAGAAAACAAAGGATCGTATCACTTTGATGCAGAGGCCCCTGAACACCTACACCA TAGAGGAAATGCCACCCTCCAGAGTCAGGTAAAATACAGAGAAGAATATGAGAAGAATAAGGGGAAGACC ATGCTTGACTTTGTTGAGACACCATCATATCAGTCTTCAAAGGAGGCTCAGAAGATGCAGAGCGAGAAAG TTTACAAAGAAGATTTTGAGAAGGAGATTAAAGGAAGATCCTCACTGGATTTAGACAAGACTCCTGCATT TCTACACGTGAAGCACATCAGCAACCTGATGAGGGAGAAAGAATATAAAAAAGATTTGGAAAATGAAATA AAAGGGAAAGGAATGGAACTTAGTTCAGAAGTTCTTGATATTCAGAGAGCAAAGCGAGCATCCGAAATGG CCAGCGAGAAAGAATACAAAAAGGACCTGGAGTTGGAAATTAAAGGGAAGGGGATGCAAATTGACGCTGA TACCCTTGAAATACAGCGAGTCAAAAGAGCTGCCAAGATAGCCAGTGAGAAAGACTATAAAAGAGATCTG GAGACAGAAATTAAAGGGAAAGGAATGCAGGTCAGCACAGACACGCTGGATGTTCAGAGAGCTAAGAGGG CATCTGAGATGGCCAGCCAGAAGCAATATAGGAAAGACTTGGAGAATGAAATTAAGGGGAAAGGAATGCA AGTAAACGTGGATATCCCGGACATGCTTCGAGCCAAGAGGGCGTCTGAAATCTACAGCCAGAAAAAGTAT AAAGATGAAGCTGAGAAGATGCTCTCCAACTATTCCACTGTGGCAGTCACTCCCGAGATTCAGAGAATCA AGACGACACAGCAAAACATTAGCAATGTATCTTATAAGGAGGAAGTAAGAGCTGGCACAGCCGTAAGGAA TACTCCAGAGATTGAACGAGTGAAGAAAAACCAGCACAATATTAGTTCGGTGAAATTTGAAGAAGGAATT AAGCATGCAACAGCCATTTCTGATCCTCTTGAGCTAAAGAGAGTTACAGAAAACCAGAAGGACGTCATCA ATAGGTTCCAGTATAAAGAGCCAACCTATAAGGCCACTCCGGTAACCATGACCCCAGAGATAGAGAGAGT GCGGAGGAACCAGGAACAGCTGAGTGCGGTCAAACACAAAGGAGAGCTAAAACAAGCAACTTCCATTTTG GATCTGCCAGGGCTGAAGAGAGTGAGAGAAAACCAGAAAACTATCAGCAATGTTTATTATAAAGGCCAAT TGGGAAGGGCTACAGCTTTGAGCGTAACGCCCGAAATGGAAAGAGTAAAGAAGAATCAAGAAAATATCAG CTCGGTGAAATACACTCAGGACCATAAACAAATGAAAGGTAGACCATGTGTGATCTTAGATACACCTGCT TTGAGACATGTAAAAGAAGCACAAAATCATGTTTCCATGGTCAAATATCATGAAGATTTTGAGAAGACGA AGGGAAGAGGCTTCACTCCTGTTGTGGACGATCCTGTGACGGAGCGGGTGAGGAAGAACACCCAGGTTGT GAGCGATGCTGCCTATAAAGGGGTACAGCCTCATGTTGTGGAGATGGATAGAAGACCTGGGATCATTGTT GACCTTAAAGTGTGGCGTACAGATCCCGGATCCATCTTCGACATTGACCCCCTGGAGGACAATATTCAGT CTCGAAGTCTCCATATGCTCTCTGAAAAGGCAAGTCAGTACCAGAGCCGCCTGTCTCGCTCACACTCTGG CAGCACTTTTGGTACGGGTCTGGGAGATGACAAGTCAGAGATCTCCGAGCTCTACCCTAGCTTTTCCTGT TGCAGTGAGGTAACAAGACCATCTGATGAAGGAGCACCCGTCCTTCCTGGAGCTTACCAGCAGAGCCACT CCCAAGGCTACGGGTACATGCACCAGACCAGCGTGTCGTCCGTGAGGTCAATGCAGCATTCAGCAAATCT AAGGACCTACCGAGCCATGTACGATTACAGTGCCCAGGACGAAGATGAGGTCTCCTTCCGAGATGGTGAC TACATCGTCAACGTGCAGCCCATTGATGACGGCTGGATGTATGGCACCGTGCAGAGAACCGGGAGAACGG GCATGCTCCCAGCGAATTACATTGAGTTTGTGAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217412 representing NM_001191694 Red=Cloning site Green=Tags(s) MKVPVSGDVKEETEEENVEEEEKPEDEVFLKPVVEDLSMELARKCTELISDIHYKEDYRKSKDKCTSVTD TPTLNHVKNISAFISETKYKGTIKADLSNCLYKDMPATIDSVFAREVSQLQSEVAYKQKHEAEKGLSDYA HMKEPPEVRHAMEVNRHQSNISYRKDVQGTHTYTAEMDRPDIKKATQISKIISNAEYKKGQGIVNKEPSV IGRPDFEHAVEASKLSSQVKYKEKFDNEMKGKGHHYNPLGSASFRQHQLATVLASDVKYKKDVQTMHEPV SDLPNLLFLDHALKASRMLSGWEYKKNFEENKGSYHFDAEAPEHLHHRGNATLQSQVKYREEYEKNKGKT MLDFVETPSYQSSKEAQKMQSEKVYKEDFEKEIKGRSSLDLDKTPAFLHVKHISNLMREKEYKKDLENEI KGKGMELSSEVLDIQRAKRASEMASEKEYKKDLELEIKGKGMQIDADTLEIQRVKRAAKIASEKDYKRDL ETEIKGKGMQVSTDTLDVQRAKRASEMASQKQYRKDLENEIKGKGMQVNVDIPDMLRAKRASEIYSQKKY KDEAEKMLSNYSTVAVTPEIQRIKTTQQNISNVSYKEEVRAGTAVRNTPEIERVKKNQHNISSVKFEEGI KHATAISDPLELKRVTENQKDVINRFQYKEPTYKATPVTMTPEIERVRRNQEQLSAVKHKGELKQATSIL DLPGLKRVRENQKTISNVYYKGQLGRATALSVTPEMERVKKNQENISSVKYTQDHKQMKGRPCVILDTPA LRHVKEAQNHVSMVKYHEDFEKTKGRGFTPVVDDPVTERVRKNTQVVSDAAYKGVQPHVVEMDRRPGIIV DLKVWRTDPGSIFDIDPLEDNIQSRSLHMLSEKASQYQSRLSRSHSGSTFGTGLGDDKSEISELYPSFSC CSEVTRPSDEGAPVLPGAYQQSHSQGYGYMHQTSVSSVRSMQHSANLRTYRAMYDYSAQDEDEVSFRDGD YIVNVQPIDDGWMYGTVQRTGRTGMLPANYIEFVN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191694
ORF Size	3045 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191694.1, NP_001178623.1
RefSeq Size	3127 bp
RefSeq ORF	3048 bp
Locus ID	307189
MW	116.1 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.