Hdac5 (NM_053450) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217483 representing NM_053450 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTCTCCCAACGAGTCGGATGGCATGTCAGGCCGGGAACCATCCTTGGAAATCTTGCCACGGACTC CTCTGCACAGCATCCCTGTGGCAGTGGAGGTGAAGCCGGTGCTGCCAGCAGCCATGCCCAGCTCCATGGG AGGTGGAGGTGGAGGTAGCCCCAGCCCTGTGGAGCTTCGGGGGGCTCTGGCAGGCCCCATGGACCCCGCG CTTCGGGAGCAGCAACTGCAGCAAGAGCTCCTGGTGCTCAAGCAGCAGCAGCAGCTGCAAAAGCAGCTCC TGTTTGCCGAGTTTCAGAAGCAGCACGACCACCTGACGCGGCAGCATGAAGTCCAGCTGCAGAAGCACCT CAAGCAGCAGCAGGAGATGCTGGCGGCTAAGAGGCAGCAGGAGCTGGAGCAGCAGCGGCAGCGCGAGCAG CAGCGGCAAGAGGAGCTAGAGAAACAGCGGCTGGAGCAGCAGCTGCTTATTCTACGAAACAAGGAGAAGA GCAAAGAGAGTGCCATCGCCAGCACCGAGGTAAAGCTGAGGCTCCAGGAATTCCTGTTGTCCAAGTCAAA GGAGCCCACACCAGGCGGCCTCAACCATTCCCTCCCACAGCACCCCAAATGCTGGGGAGCCCATCATGCT TCTTTGGACCAGAGTTCCCCTCCCCAGAGCGGCCCTCCTGGGACGCCTCCCTCCTACAAATTGCCTTTGC TTGGGCCCTATGACAGCCGTGATGACTTTCCCCTCCGTAAAACGGCCTCGGAACCCAACTTAAAAGTACG TTCAAGGCTAAAACAGAAGGTAGCTGAGAGAAGAAGCAGTCCCCTCCTGCGTCGAAAGGATGGCACTGTT ATTAGTACTTTTAAGAAGAGAGCAGTCGAGATCACAGGCACAGGGCCTGGGGTGTCGTCCGTGTGTAACA GTGCGCCTGGCTCTGGCCCCAGCTCTCCCAACAGTTCCCACAGCACCATCGCTGAGAACGGCTTTACTGG CTCAGTCCCCAACATCCCCACTGAGATGCTCCCCCAGCACCGGGCCCTCCCTCTGGACAGTTCCCCAAAC CAGTTCAGCCTCTACACGTCTCCTTCTCTGCCTAACATCTCCCTAGGGCTGCAGGCCACTGTCACTGTTA CCAACTCGCACCTCACCGCCTCCCCGAAGCTGTCGACACAGCAGGAGGCTGAGAGGCAGGCCCTTCAGTC CCTGCGGCAGGGCAGCACACTGACCGGCAAGTTCATGAGCACATCCTCCATCCCTGGCTGCCTGCTGGGA GTGGCCCTGGAGGGCGACACGAGCCCCCATGGGCACGCTTCCCTGCTGCAGCACGTTTTGCTGCTGGAGC AGGCCCGGCAACAGAGCACGCTCATAGCGGTGCCACTCCATGGGCAGTCCCCACTGGTGACGGGTGAACG TGTGGCCACCAGCATGAGGACAGTGGGCAAGCTCCCAAGGCACCGACCTCTGAGCCGTACTCAGTCCTCG CCCCTGCCGCAGAGTCCCCAGGCCCTGCAGCAGCTGGTTATGCAGCAGCAGCACCAGCAGTTCCTAGAGA AACAGAAGCAGCAGCAGATGCAGCTGGGCAAGATCCTTACCAAAACTGGGGAGCTGTCAAGGCAGCCCAC CACCCACCCTGAAGAGACAGAGGAGGAGCTGACGGAACAGCAGGAGGCCTTGCTGGGGGAGGGGGCCCTG ACCATGCCCCGGGAAGGTTCTACAGAGAGCGAGAGCACCCAGGAAGATCTAGAAGAGGAAGAGGATGAGG AGGAGGACGAGGAGGACTGCATTCAGGTCAAGGATGAGGATGGCGAGAGTGGTCCTGATGAGGGCGCAGA CTTGGAGGAGTCCAGTGCTGGTTACAAAAAGTTGTTCGCAGATGCCCAGCAGTTACAGCCCCTCCAGGTA TACCAGGCACCCCTCAGCCTGGCCACTGTGCCTCATCAGGCCCTGGGCCGCACCCAGTCCTCACCTGCTG CTCCTGGGAGCATGAAGAGCCCCACAGACCACCCCACTGTGGTGAAGCACCTCTTCACCACAGGTGTGGT CTATGACACGTTCATGCTGAAGCATCAGTGTATGTGCGGAAACACACATGTGCACCCAGAGCACGCCGGC CGCATCCAGAGCATCTGGTCCCGGCTGCAGGAAACTGGTCTGCTCAGCAAGTGTGAGCGGATCCGGGGTC GTAAAGCCACACTGGATGAAATCCAGACCGTGCACTCTGAGTACCACACCCTGCTCTATGGGACCAGTCC CCTTAACCGGCAGAAGCTGGACAGTAAGAAGCTGCTTGGCCCCATCAGCCAGAAGATGTACGCCATGCTG CCTTGTGGGGGCATTGGGGTGGACAGTGACACTGTGTGGAATGAGATGCACTCCTCTAGTGCCGTGCGAA TGGCAGTGGGCTGCCTGGTGGAGCTGGCCTTCAAGGTGGCTGCAGGAGAGCTCAAGAATGGATTTGCCAT CATCCGACCCCCAGGACATCATGCTGAGGAGTCCACAGCCATGGGATTCTGCTTCTTCAACTCCGTAGCC ATCACAGCTAAACTCCTGCAGCAGAAGCTGAGCGTGGGCAAGGTCCTCATCGTGGACTGGGACATTCACC ACGGCAATGGCACCCAGCAAGCATTTTACGATGACCCCTCCGTGCTCTACATCTCTTTGCATCGCTACGA CAATGGGAACTTCTTTCCAGGCTCCGGGGCTCCTGAAGAGGTTGGTGGAGGGCCAGGTGTGGGGTACAAC GTAAATGTGGCGTGGACAGGAGGTGTGGATCCCCCCATTGGAGATGTGGAATACCTGACAGCCTTCAGGA CAGTAGTGATGCCCATTGCCCACGAGTTCTCACCCGACGTCGTTCTAGTCTCCGCTGGGTTTGATGCTGT TGAAGGACATCTGTCTCCACTGGGTGGCTATTCTGTCACCGCCAGATGTTTTGGCCACTTGACCAGGCAG CTCATGACACTGGCAGGGGGCCGGGTGGTGCTGGCCCTGGAGGGAGGTCATGACTTGACCGCCATCTGTG ATGCTTCTGAGGCCTGTGTCTCGGCTCTGCTCAGCGTGGAGCTGCAGCCCTTGGATGAAGCAGTCTTGCA GCAAAAGCCCAGCGTCAATGCAGTTGCCACACTAGAGAAAGTCATCGAGATCCAGAGCAAACACTGGAGC TGTGTACAGAGGTTTGCCACTGGTCTGGGCTGCTCGCTGCGGGAGGCTCAGACGGGTGAGAAAGAGGAGG CCGAGACTGTGAGCGCCATGGCCCTGCTTTCCATGGGGGCTGAGCAGGCCCAGGCTGCTGCCACTCAAGA GCACAGCCCCCGGCCAGCCGAGGAGCCCATGGAGCAGGAGCCTGCCCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217483 representing NM_053450 Red=Cloning site Green=Tags(s) MNSPNESDGMSGREPSLEILPRTPLHSIPVAVEVKPVLPAAMPSSMGGGGGGSPSPVELRGALAGPMDPA LREQQLQQELLVLKQQQQLQKQLLFAEFQKQHDHLTRQHEVQLQKHLKQQQEMLAAKRQQELEQQRQREQ QRQEELEKQRLEQQLLILRNKEKSKESAIASTEVKLRLQEFLLSKSKEPTPGGLNHSLPQHPKCWGAHHA SLDQSSPPQSGPPGTPPSYKLPLLGPYDSRDDFPLRKTASEPNLKVRSRLKQKVAERRSSPLLRRKDGTV ISTFKKRAVEITGTGPGVSSVCNSAPGSGPSSPNSSHSTIAENGFTGSVPNIPTEMLPQHRALPLDSSPN QFSLYTSPSLPNISLGLQATVTVTNSHLTASPKLSTQQEAERQALQSLRQGSTLTGKFMSTSSIPGCLLG VALEGDTSPHGHASLLQHVLLLEQARQQSTLIAVPLHGQSPLVTGERVATSMRTVGKLPRHRPLSRTQSS PLPQSPQALQQLVMQQQHQQFLEKQKQQQMQLGKILTKTGELSRQPTTHPEETEEELTEQQEALLGEGAL TMPREGSTESESTQEDLEEEEDEEEDEEDCIQVKDEDGESGPDEGADLEESSAGYKKLFADAQQLQPLQV YQAPLSLATVPHQALGRTQSSPAAPGSMKSPTDHPTVVKHLFTTGVVYDTFMLKHQCMCGNTHVHPEHAG RIQSIWSRLQETGLLSKCERIRGRKATLDEIQTVHSEYHTLLYGTSPLNRQKLDSKKLLGPISQKMYAML PCGGIGVDSDTVWNEMHSSSAVRMAVGCLVELAFKVAAGELKNGFAIIRPPGHHAEESTAMGFCFFNSVA ITAKLLQQKLSVGKVLIVDWDIHHGNGTQQAFYDDPSVLYISLHRYDNGNFFPGSGAPEEVGGGPGVGYN VNVAWTGGVDPPIGDVEYLTAFRTVVMPIAHEFSPDVVLVSAGFDAVEGHLSPLGGYSVTARCFGHLTRQ LMTLAGGRVVLALEGGHDLTAICDASEACVSALLSVELQPLDEAVLQQKPSVNAVATLEKVIEIQSKHWS CVQRFATGLGCSLREAQTGEKEEAETVSAMALLSMGAEQAQAAATQEHSPRPAEEPMEQEPAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053450
ORF Size	3339 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053450.1, NP_445902.1
RefSeq Size	3754 bp
RefSeq ORF	3342 bp
Locus ID	84580
MW	121.1 kDa
Gene Summary	mouse homolog is involved in mediating nuclear receptor repression [RGD, Feb 2006]

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