Thbs2 (NM_001169138) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	TSP-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217522 representing NM_001169138 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCTGGGCACTGGCCCTGCTGGCTCTGGGCATAGGGCCAAGAGCTTACGCTGGTGACCACGGCGAGG ACACAGCATTTGACCTTTTCAGCATCAGCAACATTAACCGGAAGACCATCGGTGCCAAGCAGTTCCGAGG GCCCGACCCCGGTGTGCCCGCCTACCGTTTTGTCCGGTTTGACTACGTCCCCCCAGTGAATACAGACGAC CTCAACAGGATCGTTAAGCTTGCAAGGAGAAAGGAGGGCTTCTTCCTCACAGCCCAGCTGAAGCAGGACC GCAAGTCTCGGGGAACACTCCTGGTGTTGGAAGGTCCCGGCACCTCCCAGAGGCAGTTTGAGATTGTGTC CAATGGCCCAGGGGATACCTTGGACCTCAACTACTGGGTAGAAGGCCACCAGCATACCAACTTCCTGGAG GATGTGGGTCTGGCTGACTCCCAGTGGAAGAATGTGACTGTGCAGGTGGCCAGTGACACCTATAGCCTGT ATGTGGGCTGTGATCTTATTGACAGTGTCACCCTGGAAGAACCCTTCTATGAGCAGCTGGAAGCAGACAA GAGCAGGATGTACGTGGCCAAGGGTGCATCTCGAGAGAGTCACTTCAGGGGCTTGCTGCAGAATGTCCAT CTCGTGTTTGCAGATTCTGTGGAAGATATTCTAAGCAAGAAAGGCTGTCAACACAGCCAGGGAGCTGAAG TCAACACCATCAGTGAACATACAGAGACACTCCACCTGAGCCCTCACATCACCACAGATCTGGTGGTCCA GGGTGTGGAGAAGGCACAGGAGGTGTGTACGCACTCCTGTGAAGAGCTGAGCAACATGATGAACGAGCTG TCCGGATTGCACGTCATGGTGAACCAGCTGAGCAAGAACCTGGAGAGAGTGTCTAGCGATAACCAGTTCC TTTTGGAGCTCATTGGGGGCCCTCTGAAGACAAGAAACATGTCGGCCTGTGTGCAGGAGGGACGGATCTT TGCAGAAAACGAAACCTGGGTTGTGGATAGTTGTACCACCTGCACCTGCAAGAAATTTAAAACAGTCTGC AATCAAATCACCTGCTCACCTGCAACTTGTGCCAACCCATCTTTGGTGGAAGGCGAGTGCTGTCCATCCT GTTCCCACTCTGCAGACAACGATGAGGGCTGGTCTCCGTGGGCAGAGTGGACCGAGTGTTCTGTCACCTG TGGCTCTGGGACCCAGCAGAGAGGCCGGTCTTGTGATGTCACCAGCAACACCTGCCTGGGCCCCTCCATT CAGACAAGGACATGCAGTTTGGGCAAATGTGATACGAGAATCCGTCAGAATGGAGGCTGGAGTCACTGGT CACCCTGGTCTTCGTGCTCCGTGACTTGTGGAGTTGGCAATGTCACCCGCATACGTCTCTGCAACTCACC AGTGCCCCAAATGGGTGGCAAGAACTGCAAGGGCAGTGGCCGGGAAACCAAGGCCTGCCAGCGTGCTCCA TGCCCAATTGATGGCCGCTGGAGCCCCTGGTCCCCTTGGTCAGCCTGCACAGTTACCTGTGCTGGAGGGA TCCGTGAGCGCACACGTGTTTGCAACAGCCCTGAGCCCCAGTATGGAGGGAAGGATTGTGTCGGGGATGT GACAGAACACCAAATGTGCAACAAGAGGAGCTGCCCTATTGATGGATGCCTATCCAACCCGTGTTTCCCT GGAGCCAAGTGCAACAGCTTCCCCGATGGCTCCTGGTCCTGTGGTTCCTGCCCAGTGGGCTTTCTGGGCA ATGGCACCCACTGTGAGGACCTGGATGAGTGTGCTGTGGTCGCAGATATCTGCTTCTCAATTAACAAAGC TTCCCGCTGTGTCAACACCAACCCTGGCTTCCACTGCCTGCCTTGTCCGCCACGCTACAAGGGGACCCAA CCCTTCGGGATTGGCCTGGAGGATGCTAAAACAGAAAAACAAGTATGTGAGCCAGAGAATCCGTGCAAGG ACAAGACTCACAACTGCCACAAGCATGCAGAGTGCATCTACCTGGGCCACTTCAGTGACCCCATGTACAA GTGCGAGTGCCAGACTGGCTATGCAGGTGATGGGCTCATCTGCGGGGAGGACTCAGACCTGGACGGTTGG CCCAACAGCAACCTGGTGTGTGCTACCAATGCCACCTACCACTGCGTCAAGGACAACTGCCCCAAACTGC CAAATTCTGGGCAGGAGGATTTTGATAAGGACGGAATCGGAGATGCTTGCGACGAGGACGATGATAACGA CGGCGTGAGCGATGAGAAGGACAATTGCCCACTTCTCTTCAATCCCCGCCAATTAGACTATGATAAGGAT GAGGTTGGAGACCGCTGTGACAATTGCCCCTATGTGCACAACCAAGCACAGATCGACACGGACAACAACG GTGAAGGGGACGCCTGCTCTGTGGATATCGACGGGGATGATGTTTTCAATGAGCGAGACAATTGCCCGTA TGTCTACAACACTGACCAGAGAGACACAGACGGTGACGGCGTTGGGGACCACTGTGACAACTGTCCTCTG ATGCATAACCCAGATCAGATGGATCAGGACAATGATCTCGTTGGAGACCAGTGTGATAACAATGAGGACA TAGATGATGACGGCCACCAGAACAACCAAGACAACTGCCCATACATCTCCAACTCCAACCAGGCTGACCA TGACAATGACGGCAAGGGGGATGCCTGTGACTCCGATGATGACAACGATGGTGTTCCAGATGACAGGGAC AACTGCAGGCTTGTGTTCAACCCCGACCAGGAAGACTCGGATGGCGACGGCCGAGGTGATATTTGTAAAG ATGACTTTGACAATGATAATGTCCCAGATATTGATGACGTGTGCCCCGAGAACAATGCCATCACCGAGAC AGACTTCAGAAACTTTCAGATGGTCCCTCTGGATCCCAAGGGGACCACACAAATTGATCCCAACTGGGTA ATTCGTCACCAAGGCAAAGAGCTGGTGCAGACAGCAAACTCGGACCCTGGCATCGCCGTAGGTTTCGATG AGTTTGGGTCTGTGGACTTCAGCGGCACTTTCTATGTCAACACTGACCGGGACGATGACTACGCCGGCTT CGTCTTTGGCTATCAGTCAAGCAGCCGCTTCTATGTGGTGATGTGGAAGCAGGTGACCCAGACCTACTGG GAAGACAAGCCCAGTCGGGCTTACGGCTACTCTGGTGTGTCACTCAAAGTAGTAAACTCCACGACTGGTA CTGGCGAGCACCTGAGGAATGCCCTGTGGCACACGGGAAACACGGAAGGCCAGGTTCGAACTCTATGGCA TGACCCCAAAAACATTGGCTGGAAGGACTACACCGCCTACAGGTGGCATCTGATTCACAGGCCTAAGACA GGCTACATGAGAGTCTTAGTGCATGAAGGAAAGCAGGTCATGGCTGACTCAGGACCAATTTATGACCAAA CTTATGCTGGTGGACGGCTGGGTCTGTTTGTCTTCTCCCAAGAAATGGTCTACTTCTCAGACCTCAAGTA TGAGTGCAGAGATGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217522 representing NM_001169138 Red=Cloning site Green=Tags(s) MLWALALLALGIGPRAYAGDHGEDTAFDLFSISNINRKTIGAKQFRGPDPGVPAYRFVRFDYVPPVNTDD LNRIVKLARRKEGFFLTAQLKQDRKSRGTLLVLEGPGTSQRQFEIVSNGPGDTLDLNYWVEGHQHTNFLE DVGLADSQWKNVTVQVASDTYSLYVGCDLIDSVTLEEPFYEQLEADKSRMYVAKGASRESHFRGLLQNVH LVFADSVEDILSKKGCQHSQGAEVNTISEHTETLHLSPHITTDLVVQGVEKAQEVCTHSCEELSNMMNEL SGLHVMVNQLSKNLERVSSDNQFLLELIGGPLKTRNMSACVQEGRIFAENETWVVDSCTTCTCKKFKTVC NQITCSPATCANPSLVEGECCPSCSHSADNDEGWSPWAEWTECSVTCGSGTQQRGRSCDVTSNTCLGPSI QTRTCSLGKCDTRIRQNGGWSHWSPWSSCSVTCGVGNVTRIRLCNSPVPQMGGKNCKGSGRETKACQRAP CPIDGRWSPWSPWSACTVTCAGGIRERTRVCNSPEPQYGGKDCVGDVTEHQMCNKRSCPIDGCLSNPCFP GAKCNSFPDGSWSCGSCPVGFLGNGTHCEDLDECAVVADICFSINKASRCVNTNPGFHCLPCPPRYKGTQ PFGIGLEDAKTEKQVCEPENPCKDKTHNCHKHAECIYLGHFSDPMYKCECQTGYAGDGLICGEDSDLDGW PNSNLVCATNATYHCVKDNCPKLPNSGQEDFDKDGIGDACDEDDDNDGVSDEKDNCPLLFNPRQLDYDKD EVGDRCDNCPYVHNQAQIDTDNNGEGDACSVDIDGDDVFNERDNCPYVYNTDQRDTDGDGVGDHCDNCPL MHNPDQMDQDNDLVGDQCDNNEDIDDDGHQNNQDNCPYISNSNQADHDNDGKGDACDSDDDNDGVPDDRD NCRLVFNPDQEDSDGDGRGDICKDDFDNDNVPDIDDVCPENNAITETDFRNFQMVPLDPKGTTQIDPNWV IRHQGKELVQTANSDPGIAVGFDEFGSVDFSGTFYVNTDRDDDYAGFVFGYQSSSRFYVVMWKQVTQTYW EDKPSRAYGYSGVSLKVVNSTTGTGEHLRNALWHTGNTEGQVRTLWHDPKNIGWKDYTAYRWHLIHRPKT GYMRVLVHEGKQVMADSGPIYDQTYAGGRLGLFVFSQEMVYFSDLKYECRDA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001169138
ORF Size	3516 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001169138.1, NP_001162609.1
RefSeq Size	5728 bp
RefSeq ORF	3519 bp
Locus ID	292406
MW	129.7 kDa

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