Slc4a7 (NM_001270861) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	NBC3; NBCn1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217549 representing NM_001270861 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCAGACGGGGCCGGCGAGCAGATGAGACCGCTACTCACGCGGGGTCCCGATGAAGAAGCTGTTG TGGATCTTGGCAAAACTAGCTCAACCGTGAACACCAAGTTTGAAAAAGAAGAATTAGAGAGTCATCGAGC TGTATATGTTGGTGTTCATGTACCGTTTAGTAAAGAGAGTCGTCGGCGTCACAGGCATCGAGGGCACAAA CATCACCACCGGAGAAGAAAAGACAAAGACTCAGATAAGGAAGATGGACGGGAGTCTCCTTCTTATGACA CGCCATCGCAGAGGGTGCAGTTCATCCTTGGGACTGAAGACGATGATGAGGAGCACATTCCCCACGACCT CTTCACGGAGATGGACGAGCTCTGCTACCGAGACGGGGAAGAGTACGAGTGGAAAGAGACAGCCAGGTGG CTGAAGTTCGAAGAGGATGTTGAGGATGGTGGTGACCGATGGAGTAAACCGTATGTGGCCACTCTGTCTT TGCACAGTCTCTTTGAGCTGAGGAGTTGTATCCTAAATGGAACAGTCATGCTGGATATGAGAGCAAGCAC TCTGGATGAAATAGCAGATATGGTGTTAGACAACATGATAGCGTCTGGCCAGCTAGATGACTCCATAAGG GAGAATGTTCGAGAAGCTCTTCTGAAGAGACATCATCATCAAAATGAGAAAAGGTTTACAAGTCGGATTC CCCTCGTTCGATCCTTTGCAGATATAGGCAAGAAACATTCTGACCCTCACTTGCTTGAAAGGAATGGGGA AGGCCTGTCAGCCTCCCGCCATTCTTTGCGAACAGGTCTGTCTGCCTCAAACCTTTCCTTGAGAGGAGAA TCGCCTTTATCCCTTCTTCTCAGTCATCTTCTTCCTTCTTCAAGAGCTGGCACCCCTGCAGGCTCAAGGT GTACCACCCCAGTACCCACCCCCCAGAACAGTCCTCCTTCCAGCCCCAGCTTAAGTCGCCTGACCTCCAG AAGTTCCCAACAGACTCAGCCTCAGGCCCCAGAAGTACTGGTGTCACCTGACAGGGATGATATTCCCAGA GTAGTAATTCATCCGCCTGAGGAAGACATAGAAGCACTGAAAGGCCAAGAGCAGAAGAATGAGGAAAATA CTGACTTCACTCCAGGGATTTTGGCTTCTCCACAGTCTGCTCCTGGAAACCTGGACAGTAGTAAAAGTGG TGAAGTGAAAGGTAACGGAAGTGGAGGAAGCAGAGAAAATAGTACTGTTGACTTTAGCAAGGTTGACATG AATTTCATGAGGAAAATTCCTACAGGAGCTGAGGCATCCAATGTTTTGGTAGGAGAGGTGGATTTCTTGG AGAGACCTATCATTGCATTTGTGAGACTGGCTCCTGCAGTTCTCCTCTCAGGGTTGACTGAGGTCCCTGT GCCCACTAGGTTTTTGTTTCTGTTACTGGGCCCAGCAGGAAAGGCTCCACAGTACCATGAAATTGGCAGA TCCATAGCAACTCTAATGACAGATGAGATTTTTCATGATGTAGCTTATAAAGCAAAAGATCGAAATGACC TCTTATCTGGAATTGATGAATTTTTAGATCAAGTAACTGTTCTTCCTCCAGGAGAGTGGGATCCTTCCAT ACGCATAGAGCCACCAAAAAGTGTTCCTTCTCAGGAGAAAAGGAAGATTCCTGCGTTTCCCAATGGATCT GCTCCAGTGTCTGCTGACCCTCCTAAGGAGGCTGATCACCACGCTGGGCCTGAGCTGCAGAGGACTGGAC GGCTTTTTGGTGGTTTGATACTTGACATCAAAAGGAAAGCACCTTTTTTCTTGAGTGACTTCAAGGATGC ATTAAGTCTGCAGTGCCTGGCCTCGATTCTTTTCCTATACTGTGCCTGTATGTCTCCTGTAATCACTTTT GGAGGGCTGCTTGGAGAAGCTACAGAAGGCAGAATAAGTGCAATAGAGTCTCTTTTTGGAGCATCATTAA CTGGGATTGCCTATTCATTGTTTGCTGGGCAACCTCTAACAATACTGGGGAGCACGGGTCCAGTCCTAGT GTTTGAAAAAATTTTATTTAAATTCTGTAGAGATTATCACCTATCCTATCTATCATTAAGAACCAGTATT GGTCTGTGGACTTCTTTCTTGTGCATTGTGTTGGTCGCAACAGATGCCAGCAGCCTTGTTTGTTACATTA CTCGGTTCACAGAAGAAGCTTTCGCCGCACTCATTTGTATCATCTTCATCTATGAAGCCTTGGAGAAACT CTTTCACTTAGGAGAAATATATGCATTTAACATGCACAACAACTTGGATGCATTGACCAGTTACACATGT GTATGTGCTGAGCCATCTAATCCTAGCAACGAAACTGTAGAGCTGTGGGAGAGGAAGAACGTGACAGCAG CCAGTATTTCCTGGGCAAACCTTACCGTGTCTGAGTGTAAGACCTTCCACGGTATGTTTGTGGGATCAGC TTGTGGGCCTCACGGGCCTTATGTTCCCGATGTGCTCTTCTGGTGTGTCGTCTTGTTTTTCACAACGTTC TTTCTGTCTTCATTCCTCAAGCAGTTTAAGACCAAGAGATATTTTCCTACTAAGGTGCGATCAACAATCA GTGACTTTGCTGTATTTCTCACAATAGTAATAATGGTTGCAATTGACTACCTTGTAGGAATTCCATCTCC TAAACTTCATGTTCCTGAAAAGTTTGAGCCTACTGATCCAAGCAGGGGCTGGATCATAAGCCCTTTGGGA GATAACCCTTGGTGGACCTTACTAATTGCAGCTGTTCCAGCTCTCCTTTGTACCATTCTCATCTTCATGG ACCAGCAGATCACAGCTGTGATCATCAACAGGAAAGAACACAAACTGAAGAAAGGAGCTGGCTATCACCT GGACTTGCTCATGGTTGCTGTCATGTTGGGAGTCTGCTCCATCATGGGCCTGCCGTGGTTTGTGGCTGCA ACAGTGTTGTCTATAAGTCATGTCAACAGCTTAAAAGTAGAGTCTGAATGTTCTGCTCCAGGGGAACAGC CCAAGTTCCTCGGAATTCGTGAACAGCGGGTTACAGGGCTGATGATTTTTATCCTGATGGGCCTCTCTGT GTTCATGACTTCAGTATTAAAGTTTATTCCGATGCCAGTTCTATATGGTGTTTTCCTTTATATGGGAGTT TCTTCTCTGAAAGGAATTCAGTTTTTTGACCGTATCAAATTATTTGGAATGCCTGCCAAGCACCAGCCGG ACCTGATCTACCTCCGCTATGTCCCTCTCTGGAAGGTGCACGTGTTCACGGTCGTCCAGCTGACCTGCCT GGTTCTGCTCTGGGTGATCAAAGCCTCTGCTGCTGCAGTAGTTTTTCCCATGATGGTTCTTGCATTAGTC TTTGTGCGCAAGCTCATGGATCTGTGCTTCACAAAGAGAGAACTCAGTTGGCTTGATGACCTCATGCCAG AAAGTAAGAAAAAGAAAGAAGATGACAAGAAGAAGAAGGAGAAGGAGGAAGCTGAGCGGATGCTTCAGGG TGACGGGGATACTGTGCACCTCCCATTCGAAAGGGGGAGTCTCCTACAAATTCCAGTTAAGACCCTAAAA TATAGCCCTGAAAAGCCTGTGAGTGTGACAATAAATTTCGAAGATGAACCATCAAAAAAATACATGGATG CTGAAACTTCATTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217549 representing NM_001270861 Red=Cloning site Green=Tags(s) MEADGAGEQMRPLLTRGPDEEAVVDLGKTSSTVNTKFEKEELESHRAVYVGVHVPFSKESRRRHRHRGHK HHHRRRKDKDSDKEDGRESPSYDTPSQRVQFILGTEDDDEEHIPHDLFTEMDELCYRDGEEYEWKETARW LKFEEDVEDGGDRWSKPYVATLSLHSLFELRSCILNGTVMLDMRASTLDEIADMVLDNMIASGQLDDSIR ENVREALLKRHHHQNEKRFTSRIPLVRSFADIGKKHSDPHLLERNGEGLSASRHSLRTGLSASNLSLRGE SPLSLLLSHLLPSSRAGTPAGSRCTTPVPTPQNSPPSSPSLSRLTSRSSQQTQPQAPEVLVSPDRDDIPR VVIHPPEEDIEALKGQEQKNEENTDFTPGILASPQSAPGNLDSSKSGEVKGNGSGGSRENSTVDFSKVDM NFMRKIPTGAEASNVLVGEVDFLERPIIAFVRLAPAVLLSGLTEVPVPTRFLFLLLGPAGKAPQYHEIGR SIATLMTDEIFHDVAYKAKDRNDLLSGIDEFLDQVTVLPPGEWDPSIRIEPPKSVPSQEKRKIPAFPNGS APVSADPPKEADHHAGPELQRTGRLFGGLILDIKRKAPFFLSDFKDALSLQCLASILFLYCACMSPVITF GGLLGEATEGRISAIESLFGASLTGIAYSLFAGQPLTILGSTGPVLVFEKILFKFCRDYHLSYLSLRTSI GLWTSFLCIVLVATDASSLVCYITRFTEEAFAALICIIFIYEALEKLFHLGEIYAFNMHNNLDALTSYTC VCAEPSNPSNETVELWERKNVTAASISWANLTVSECKTFHGMFVGSACGPHGPYVPDVLFWCVVLFFTTF FLSSFLKQFKTKRYFPTKVRSTISDFAVFLTIVIMVAIDYLVGIPSPKLHVPEKFEPTDPSRGWIISPLG DNPWWTLLIAAVPALLCTILIFMDQQITAVIINRKEHKLKKGAGYHLDLLMVAVMLGVCSIMGLPWFVAA TVLSISHVNSLKVESECSAPGEQPKFLGIREQRVTGLMIFILMGLSVFMTSVLKFIPMPVLYGVFLYMGV SSLKGIQFFDRIKLFGMPAKHQPDLIYLRYVPLWKVHVFTVVQLTCLVLLWVIKASAAAVVFPMMVLALV FVRKLMDLCFTKRELSWLDDLMPESKKKKEDDKKKKEKEEAERMLQGDGDTVHLPFERGSLLQIPVKTLK YSPEKPVSVTINFEDEPSKKYMDAETSL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001270861
ORF Size	3654 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270861.1, NP_001257790.1
RefSeq Size	3933 bp
RefSeq ORF	3657 bp
Locus ID	117955
UniProt ID	Q9R1N3
MW	136 kDa
Gene Summary	regulates intracellular pH in different cells along with Na+-driven HCO3- transporters, the Na+-driven Cl-/HCO3- exchanger [RGD, Feb 2006]

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