Adamts18 (NM_001191944) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1560118
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217550 representing NM_001191944 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTGCGCCCTCCTGTTCCTGTGCGCCCTCCGGGCCGCCGGTCCTGGGCCGCCGTGGGGGCCCGCGG GACTGGCGCGTCTGGCCAAGGCGCTCCAGCTGTGCTGCCTCTGCTGTGCTTCGGTGGCCGTCGCCTTAGC CAGTGACAGCGGCAGCAGCGGTGGCAGCGGATTAAATGATGATTACGTCTTTGTCGTGCCAGTAGAAGTG GACTCGGGCGGGTCATATATTTCGCACGACATTTTGCACAACAGGAAAAGGCGATCGGCACACGGTGCCA GCAGCTCCGTGCACTACCGCTTTTCAGCATTTGGACAGGATCTACACTTAGAGCTTAAGCCCTCGGCGCT TTTGAGCAGTCACTTTATAGTCCAGGTACTTGGAAAGGATGGTGCTTCAGAGACTCGGGAGCCTGCGGTG GTACAGCAGTGTTTGTATCAGGGATTTATCAGAAATGACAGCCTGTCCTCTGTTGCTGTGTCTACGTGTG CCGGCTTGTCAGGTCTGATAAGGACACGAGACAATGAATTCCTCATCTCACCGCTACCTCAGCTGCTGGC CCAGCAACACAACTACAGCTTGCCTGCAGGCCACCATCCTCACGTCCTGTACAAAAGGACAGCAGAGAAG AGGGTCAGGAGGTATCAAGACTACCCTGCCTCCCAGCGGACATATCCTGGTCACTCCCCAAGTCACACCC CCCCTGCCTCCCAGAGCCAAGAGACAGAGTACCGCCATCGAAGGTGGCAAAAGCAGCATTTTTGTGGACG ACGCAAGAAATATGCCCCCAAACCTCCTGCAGAAGATGCCTATCTACGTTTTGATGAATATGGAGGCGCA GGACGGCCCAGACGATCAGCCAGAAAGTCTCAAAAGGGTCTCAACGTGGAGACCCTCGTGGTGGCAGATG CAAAGATGGTGGAGAAGCACGGCAAGGATGACGTCACTACGTACATCCTCACAGTCATGAACATGGTTTC TAGCCTGTTCAAAGATGGGACCATTGGAAGTGACATAAACATTGTGGTTGTGAGCCTAATTCTGTTGGAA GACGAACCTGGAGGATTGCTGATCAATCACCATGCAGACCAGTCTCTAAATAGCTTCTGTCAATGGCAGT CAGCCCTTGTGGGAAAGAACGGCAAGAGACACGACCATGCCATCCTCCTCACAGGATTTGACATTTGTTC CTGGAAGAACGAACCGTGTGACACCCTAGGATTTGCACCTATCAGTGGGATGTGCAGTAAATACCGAAGC TGTACCATCAATGAAGACACAGGACTTGGCCTTGCCTTCACCATTGCACATGAGTCAGGGCACAACTTTG GCATGGTTCATGATGGCGAAGGCAACCCCTGCAGGAAGGCGGAAGGCAACATCATGTCCCCCACGCTGAC CGGAAACAATGGGGTGTTTTCATGGTCCTCCTGCAGCCGTCAGTATCTAAAGAAATTCCTCAGTACACCA CAGGCTGGCTGTCTGGTGGATGAGCCCAAGCAAACGGGACAGTATAAATATCCGGACAAACTCCCAGGAC AGATTTATGATGCCGACACGCAGTGTAAGTGGCAATTTGGAGCCAAAGCCAAGCTGTGCAGCCTTGGGGT TATGAAGGACATTTGCAAGTCGCTCTGGTGCCACCGCGTGGGCCACAGATGTGAAACCAAGTTCATGCCT GCAGCCGAGGGGACCGTCTGTGGCTTGAGCATGTGGTGTCGGCAAGGCCAATGTGTAAGGCTTGGAGATC TCGGGCCCCGGCCCATCCACGGCCAGTGGTCTGCCTGGTCGAAGTGGTCAGAATGTTCTCGAACATGCGG TGGAGGAGTCAAGTACCAGGAGAGACACTGTAGTAACCCCAAGCCTCAGTATGGTGGGAAGTACTGTCCA GGATCTAGCCGCATCTACAAGCTGTGCAACATTAACCCTTGTCATGAGAATAGCTTGGATTTCCGTGCCC AGCAATGTGCAGAGTATAACAACAAGCCCTTCCGGGGATGGTTGTACCGTTGGAGACCCTATACAAAAGT TGAAGAGGAAGATCGATGTAAACTCTACTGCAAGGCTGAGAACTTTGAGTTTTTTTTCGCCATGTCTGGC AAGGTGAAAGACGGGACGCCTTGCTCCCCACACAGAAATGATGTCTGCATCGACGGGATTTGTGAACCTG TGGGATGCGACCATGAACTTGGCTCTAAGGCAGTGTCAGATGCATGCGGTGTTTGCAAAGGCGATAACTC GACTTGCAAGTTTTATAAAGGCCTGTACCTCAGTCAGCACAAAGCCAACGAGTATTATCCTGTGGTCACT ATCCCCGCTGGGGCCCGAAGCATTGAAATTCAGGAGTTGGAGCTTTCTTCCAGCTATCTTGCTGTTCGAA GCCTCAGTCAAAAGTATTACCTCACAGGGGGCTGGAACATCGACTGGCCTGGGGACTTCACCTTCGCCGG CACCACATTTGAATACCAGCGTCCCTTTAACCGCCCTGAACGACTGTACGCGCCAGGACCCACAAATGAG ACGCTGGTCTTTGAAATTCTGACCCAAGGCAAGAACCCCGGGATCACTTGGAAGTACGCACTTCCCAAGG TCATGAATGTAACTCAGCCAGCGGCCAAGAGACACCACCACACCTGGCGCACAGTACAGTCTGACTGCTC GGTCACCTGCGGCGGAGGTTACATAAGCATCAAGGCCATTTGCTTACGAGATCAAAACACCCAAGTCAAT TCCTCATTCTGCAGTGTAAGAACCAAGCCAGCAACGGAACCCAAGCTATGCAATGCTTTCTCCTGCCCAG CCTATTGGTTGCCAGGTGAGTGGAGTACATGCAGCAAGTCCTGTGCCGGGGGCCAGCAGAGCCGCAAGAT CCGGTGTGTGCAGAAGAAGCCCTTCCAGAAAGAGGAGGCTGTGCTGCATTCTCTCTGTCCAGTGAGCACG CCTACCCAGGTTCAAGTCTGCAACAGCCATGCCTGCCCTCCAGAGTGGAGCCCCGGGCCCTGGTCTCAGT GTTCCAAGACCTGTGGCCGGGGAGTGAGGAGACGTGAGGTCCTCTGTACAAGCCCTGCAGCCGAGACACT CCCCGAGAGCCTGTGTTCCAGCAGCCCCAAACCCGAGGCACAGGAGGGCTGTGTGCTGGGACGGTGCCCC AAGAACAACAGACTGCAGTGGATCGCCTCTGCCTGGACTGAGTGTTCTGCAACCTGTGGCTTGGGTGTGA GGAAGAGAGAATTCAAGTGCATGGAGAAGACCCTGCAGGGAAAGCTGATGACGTTCCCAGAGCGGAGATG TCGCAACATTAAGAAGCCAAACCTGGAACTGGAAGAAACCTGCAACCGAAGGGCTTGCCCAGTATACAGC ATGGCGGCAGGCTGGTATTCGTCCCCGTGGCAACAGTGCACTGTAACCTGTGGGGGAGGGGTCCAGACCC GATCTGTCCACTGCATGCAGCAAGGCCGGCCTTCCTCAAGTTGTCTGCTCCATCAGAAGCCTCCGGTGCT AAGAGCCTGTAATACCAACTTCTGTCCAGTTCCTGAAAAGAGAGATGATCCGTCGTGTGTGGATTTCTTC AGCTGGTGTCGCCTGGTCCCTCAGCACGGAGTCTGCAACCACAAATTTTATGGAAAACAGTGCTGTCGAT CATGCTCAAGGAAGAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217550 representing NM_001191944 Red=Cloning site Green=Tags(s) MECALLFLCALRAAGPGPPWGPAGLARLAKALQLCCLCCASVAVALASDSGSSGGSGLNDDYVFVVPVEV DSGGSYISHDILHNRKRRSAHGASSSVHYRFSAFGQDLHLELKPSALLSSHFIVQVLGKDGASETREPAV VQQCLYQGFIRNDSLSSVAVSTCAGLSGLIRTRDNEFLISPLPQLLAQQHNYSLPAGHHPHVLYKRTAEK RVRRYQDYPASQRTYPGHSPSHTPPASQSQETEYRHRRWQKQHFCGRRKKYAPKPPAEDAYLRFDEYGGA GRPRRSARKSQKGLNVETLVVADAKMVEKHGKDDVTTYILTVMNMVSSLFKDGTIGSDINIVVVSLILLE DEPGGLLINHHADQSLNSFCQWQSALVGKNGKRHDHAILLTGFDICSWKNEPCDTLGFAPISGMCSKYRS CTINEDTGLGLAFTIAHESGHNFGMVHDGEGNPCRKAEGNIMSPTLTGNNGVFSWSSCSRQYLKKFLSTP QAGCLVDEPKQTGQYKYPDKLPGQIYDADTQCKWQFGAKAKLCSLGVMKDICKSLWCHRVGHRCETKFMP AAEGTVCGLSMWCRQGQCVRLGDLGPRPIHGQWSAWSKWSECSRTCGGGVKYQERHCSNPKPQYGGKYCP GSSRIYKLCNINPCHENSLDFRAQQCAEYNNKPFRGWLYRWRPYTKVEEEDRCKLYCKAENFEFFFAMSG KVKDGTPCSPHRNDVCIDGICEPVGCDHELGSKAVSDACGVCKGDNSTCKFYKGLYLSQHKANEYYPVVT IPAGARSIEIQELELSSSYLAVRSLSQKYYLTGGWNIDWPGDFTFAGTTFEYQRPFNRPERLYAPGPTNE TLVFEILTQGKNPGITWKYALPKVMNVTQPAAKRHHHTWRTVQSDCSVTCGGGYISIKAICLRDQNTQVN SSFCSVRTKPATEPKLCNAFSCPAYWLPGEWSTCSKSCAGGQQSRKIRCVQKKPFQKEEAVLHSLCPVST PTQVQVCNSHACPPEWSPGPWSQCSKTCGRGVRRREVLCTSPAAETLPESLCSSSPKPEAQEGCVLGRCP KNNRLQWIASAWTECSATCGLGVRKREFKCMEKTLQGKLMTFPERRCRNIKKPNLELEETCNRRACPVYS MAAGWYSSPWQQCTVTCGGGVQTRSVHCMQQGRPSSSCLLHQKPPVLRACNTNFCPVPEKRDDPSCVDFF SWCRLVPQHGVCNHKFYGKQCCRSCSRKS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191944
ORF Size	3657 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191944.1, NP_001178873.1
RefSeq Size	3882 bp
RefSeq ORF	3660 bp
Locus ID	361412
MW	135.5 kDa

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