Pcdh11x (NM_001271228) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1562864
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217592 representing NM_001271228 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTTGTTGTCCGGGACGTATATATTTGCGGTCCTGTTAGCATGCGTCGTGTTTCAGTCTGGCGCCC AGGAGAAGAATTATACAGTCCGAGAAGAAATGCCAGAAAACGTCCTGATAGGCGACTTGTTGAAAGACCT CAACTTGTCGCTTATTCCCGACAAGTCCTTAACCACGCCTATGCAATTCAAATTAGTTTACAAAACCGGG GATGTACCATTGATTCGGATTGAAGAGGGTACTGGCGAGATCTTCACTACTGGCGCACGCATTGATCGTG AGAAATTATGTGCTGGAATCGTGTTGGATGCTCGTTGCTTTTACGAAGTGGAGGTTGCTGTTTTGCCGGA TGAAATATTTAGATTGGTTAAGATACGCTTTCTGATAGAAGACATAAATGATAATGCACCGTTATTCCCG ACAACTGTCATCAACATATCAATTCCAGAAAACTCGGCTATAAACTCTCGATATTCTCTTCCGGCAGCCA TTGATCCTGACATTGGAATAAATGGAGTCCAAAATTACCAACTAATTAAGAGTCAAAATGTTTTCGGTCT TGATGTCGTTGAAACACCAGAAGGAGAAAAGATGCCCCAGCTGATCGTTCAAAAGGAATTAGATAGAGAA GAAAAGGATACCTATGTGATGAAAGTAAAAGTTGAAGATGGTGGCTTTCCTCAAAGATCCAGTACAGCTA TTTTGCAAGTAAGTGTTGCTGATACAAACGACAACCGCCCAATCTTCATAGAGAAGGAAATTGAAGTCAG TATACCAGAAAATGCTCCTATAGGTTCTTCAGTGACACAACTCCATGCCACAGATGCTGATATAGGTGAA AACGCCAGAATTCACTTTTATTTCAGCAATCTAGTCTCCAATAACGCTAAGAGACTGTTTCATCTTAACA CCACTACCGGGCTTATCACTGTCAAAGAACCACTGGATAGGGAAGAATCACCAAGCCACAAGTTATTGGT TTTGGCAACTGATGGTGGATCGACACCAGCAAAAGCAATGGTGCTGGTAAATGTTACAGATATCAATGAT AATGTACCATCAATTGACATAAGATACATCGTCAATCCAACCAATGGCACTGTGCTCCTTTCAGAGAATG CTCCGCTTAACACTAAAATTGCTCTCATAACTGTGACGGATAAGGATTCTGAACATAATGGTAGGGTGAC ATGCTTCACAGATCATGAAGTTCCTTTCAGATTACGTCCAGTATTCAGTAATCAGTTTCTCTTGGAGACA GCTGCATTTCTTGACTTTGAGTCCACCAGAGAATATGCCATTAAATTACTGGCCGCTGATGCTGGCAAAC CTCCTTTGAATCAGTCATCCATGCTCCTGATCAAAGTAAAAGATGAAAATGACAATGCTCCGGTTTTCAC CCAATCTTTCATAAGCCTTTCCGTTCCTGAGAATAACTCTCCTGGTGCACAGTTGACAAAAATCAGTGCA ACAGATGCAGACAGTGGACGCAATGCTGAAATAAACTACCTGCTAGGTTTTGATGCACCACCTGAATTCA ACCTGGATCGTCGTACAGGCATCCTGACTGCAGTGAAGAAACTAGATAGAGAAAAACAGGAAAAGTATTA CTTTACAGTCCTGGCACAGGACAATGGAATTCCACCCTTAATGTCCAATGCCACTGTGTTCGTGACTGTT CTTGACCAGAATGATAACAGCCCAATTTTCACTCATAATGAATATAACTTCTATGTTCCTGAAAACCTTC CAAAACATGGCACAGTAGGGCTAATAACTGTGACTGATCCTGATTATGGGGAGAATTCTGCAGTTACTCT CTCTGTCTTAGATGTAAACGATCAATTCACTATTGATCCACAGTCTGGTGTCATCAGGCCAAATATTTCA TTTGACAGAGAAAAACAAGAATCCTATACTTTCTATGTAAAAGCTGAGGATGGTGGTAGGGTATCACGTT CTTCAACTGCTAAAGTAACCATAAATGTGGTTGACGTAAATGATAACAAACCAATTTTTATTGACCCTCC TTCCAATTACTCCTTTGAATGGGTTCTACCATCCACAGATCCTGGCACAGTCATCTTCAAGGTTCTTGCA ATTGACAATGATACTGGCATGAATGCAGAGCTTCGTTACAGCATTGTTGGAGGAAACACAAAAGGACTGT TTATGATTGAACAAATATCAGGCAACATCACATTGAAGGAGAAGTGCATGGTTTCAGATATTGGTTTACA TCGAGTCATAGTCAAGGCTAAAGATTTAGGACAACCTGATTCTCTCTTCAATGTTGTAAATGTCAATCTC TTTGTAAATGAGTCTGTGCCCAATACTACCCTGATTTATGAAATGGTGCGCAAAAGCATTGACTCACCTG CAAATCAAAATTCTGAAACAACAAGTGCATCCTCACCAACCACCGACTATGTCAAGATCATGGTTGCCAT TGTTGCTGGCACCATAACTGTTGTCCTAGTTATTTTCATCACTGCTGTAGTAAGATGCCGCCAATCACCA CACCTTAAGGCTTCTCAGAAAAACAAACAGAATTCTGAGTGGGTTACTCCAAACCCAGAAAACAGACAGA TGATCATGATGAAGAAGAAGAAGAAGAAAAAGAAGAAGCATCCCCCCAAGAACCTGCTGCTTAATTTTGT TAGTATTGAAGAAGCAAAGCCAGATGATGCTGACAATGAGAGAAACAGTGTTACACTAGATCTTCCTATT GAGCTGGAGGATCAAACCATGGGCAAATACAACTGGGGCACTACACCTACTACCTTCAAGCCCGATAGCC CTGATTTGGCTCGACACTACAAATCTGCCTCTCCCCAGCCTGCATTCCAGATCCAGCCTGAAACACCCCT AAACTCAAAGCACCACATCATTCAGGAGCTTCCTCTTGATAATACCTTCGTTGGGTGTGATTCCATCTCC AAGTGCTCCTCCAGCAGTTCTGATCCCTACAGTGTTTCTGAGTGCAGCTATCCAGTGACAACTTTCAAGA CTCCTGTGTCTGTGCATACCAGACCTCCAATGAAGGAAGTCGTACGATCTCACACACCTATGAAAGAGGC AACAACTGTGGAAATCTGGACTCATCCGCACCCACAGCGGAGATCTGATGGAAAAAAGGCAGGAAAGTCC CAGAGACGTGTTACATTTCACCTACCAGAAGGCTCTCAGGAAAGCATCAGTGATGGTGGATTGGGAGACC ATGATCCAGGCAGCCTTCCCAGTACATCCCATGCACTGCCACTTGGCTATCCACAGGAAGAGTATTTTGA TCATGCTGCACCAAACAACCGCACTGAAGGGGATGGCAACTCTGATCCTGAATCTACTTTCATTCCTGGA CTAAAGAAAGCTGCAGAAATAACAGTGCAGCCAACTGTGGAAGAAGCCTCTGACACCTGCACTCAAGAAT GCCTCATCCTGGGCCACTCTGACTCCTGCTGGATGCCAGCTTCGCTGACCAATCCTACTCCTTCACAGAT AAAGACTTCTGCTATCTGCCACAGTCCACCCAGGACCCATTCATCAGTTCGTCGCTGCAGCCCTCCAGTG ACACAGACTGTTGCTATCTGCCACAGTCCTCCTGTGACCCAGGCTATTGCCCTGTGCCACAGCCCTCCAC CAGTACAGGCTACCGTACTCCGTCATAGCTCACCACCAGCACAGGCCTCTGCAGTCTGCTACAGCCCAAC TCTGGTACAGGCTGTGGTAATTCACCGCAGTCCTCCTCTGCCACAGGCTGCCACACTCCGTCACACTCAG GCACAACCACCAATGGGTTTACAGCAAGGATGGGTGCAGGGTGCAGGAGCAGATGGAACACATCCTATTG ATCAGGGAGTACAGGGTAGTACGAGAGCTCAGTTTTACACCATGGCTGAAAGACTTCATCCAGATGATGA CTCAATTAAAGTCATTCCTTTGACCACCTTTACTTCAGGTAAACAGGCCAGAGCCTCAAGAGGTGATTCT CCCATCATGGAGGAACATCCCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217592 representing NM_001271228 Red=Cloning site Green=Tags(s) MDLLSGTYIFAVLLACVVFQSGAQEKNYTVREEMPENVLIGDLLKDLNLSLIPDKSLTTPMQFKLVYKTG DVPLIRIEEGTGEIFTTGARIDREKLCAGIVLDARCFYEVEVAVLPDEIFRLVKIRFLIEDINDNAPLFP TTVINISIPENSAINSRYSLPAAIDPDIGINGVQNYQLIKSQNVFGLDVVETPEGEKMPQLIVQKELDRE EKDTYVMKVKVEDGGFPQRSSTAILQVSVADTNDNRPIFIEKEIEVSIPENAPIGSSVTQLHATDADIGE NARIHFYFSNLVSNNAKRLFHLNTTTGLITVKEPLDREESPSHKLLVLATDGGSTPAKAMVLVNVTDIND NVPSIDIRYIVNPTNGTVLLSENAPLNTKIALITVTDKDSEHNGRVTCFTDHEVPFRLRPVFSNQFLLET AAFLDFESTREYAIKLLAADAGKPPLNQSSMLLIKVKDENDNAPVFTQSFISLSVPENNSPGAQLTKISA TDADSGRNAEINYLLGFDAPPEFNLDRRTGILTAVKKLDREKQEKYYFTVLAQDNGIPPLMSNATVFVTV LDQNDNSPIFTHNEYNFYVPENLPKHGTVGLITVTDPDYGENSAVTLSVLDVNDQFTIDPQSGVIRPNIS FDREKQESYTFYVKAEDGGRVSRSSTAKVTINVVDVNDNKPIFIDPPSNYSFEWVLPSTDPGTVIFKVLA IDNDTGMNAELRYSIVGGNTKGLFMIEQISGNITLKEKCMVSDIGLHRVIVKAKDLGQPDSLFNVVNVNL FVNESVPNTTLIYEMVRKSIDSPANQNSETTSASSPTTDYVKIMVAIVAGTITVVLVIFITAVVRCRQSP HLKASQKNKQNSEWVTPNPENRQMIMMKKKKKKKKKHPPKNLLLNFVSIEEAKPDDADNERNSVTLDLPI ELEDQTMGKYNWGTTPTTFKPDSPDLARHYKSASPQPAFQIQPETPLNSKHHIIQELPLDNTFVGCDSIS KCSSSSSDPYSVSECSYPVTTFKTPVSVHTRPPMKEVVRSHTPMKEATTVEIWTHPHPQRRSDGKKAGKS QRRVTFHLPEGSQESISDGGLGDHDPGSLPSTSHALPLGYPQEEYFDHAAPNNRTEGDGNSDPESTFIPG LKKAAEITVQPTVEEASDTCTQECLILGHSDSCWMPASLTNPTPSQIKTSAICHSPPRTHSSVRRCSPPV TQTVAICHSPPVTQAIALCHSPPPVQATVLRHSSPPAQASAVCYSPTLVQAVVIHRSPPLPQAATLRHTQ AQPPMGLQQGWVQGAGADGTHPIDQGVQGSTRAQFYTMAERLHPDDDSIKVIPLTTFTSGKQARASRGDS PIMEEHPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271228
ORF Size	4014 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271228.1, NP_001258157.1
RefSeq Size	4385 bp
RefSeq ORF	4017 bp
Locus ID	317204
MW	147.2 kDa

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