Cdk13 (NM_001271296) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Cdc2l5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217628 representing NM_001271296 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGAGCAGCTCGGACACGGCGCTGGGGGGAGGCGGGGGCCTGAGCTGGGCCGAGAAGAAGTTGGAGG AGCGCCGCAAGCGGAGGCGATTCCTGTCCCCTCAGCAGCCGCCGCTGCTGTTGCCGCTCCTGCAGCCGCA GCTCCTGCAACCGCCGCCGCCCCCGCCGCCTCTGCTCTTCCTGGCTGCCCCCGGCGCGGCCGCCGCCGCA GCCGCCGCCGCCGCGGCCTCCTCCTCCTGCTTCAGCCCGGGCCCTCCTTTGGAGGTCAAGCGGCTGGCGA GAGGCAAGAGGCGCCCCGGAGGGCGGCAGAAGCGGCGCCGCGGGCCCCGCGCCGGGCAGGAGGCGGAGAA GCGCCGGGTCTTCTCGCTGCCCCAGCCGCAGCAGGACGGCGGTGGCGGTGCCAGTAGCGGCGGGGGTGTG ACCCCGCTGGTGGAGTATGAGGATGTAAGCTCCCAGTCCGAGCAGGGGCTGCTGCTGGGGGGCGCCAGCG CGGCAACGGCGGCGACGGCTGCCGGGGGAACGGGGGGCAACGGCGGGAGTCCGGCCTCCTCCTCCGGCAC GCAGAGGCGCGCGGAGGGGTCGACGCGCAGGCCGCGCCGGGACCGCCGCAGCAGCAGCGGTCGCAGTAAG GAGCGCCATCGCGAGCACCGGCGGCGGGACGGGACGCGCAGTGGCAGCGAGGCCTCAAAGGCCCGCAGCC GCCACGGACACAGCGGCGAGGAGCGGGCCGAGGCCGCCAAGAGCGGCAGTAGCAGCAGCAGCGGCGGCCG CCGCAAAAGTGCATCGGCTACGTCCAGCAGTAGTAGCAGCCGCAAGGATCGGGACCTCAAGGCCCATCGC AGCCGGACTAAGTCGTCCAAGGAGCCGCCCTCGGCCTACAAGGAGCCGCCCAAGGCCTACCGGGAGGACA AGAGCGAACCGAAGGCCTACAGGCGGCGGCAGCGGTCCCTGAGCCCGCTGGGAGGCCGGGACGAAAGCCC GGTGTCCCACAGGGCCTCGCAGAGCCTCCGGAGCCGCAAGTCCCCCAGCCCGGCGGGAGGTGGCAGCAGT CCCTATTCCCGGCGGTTGCCGCGCTCCCCGAGCCCCTACAGCCGGCGCCGCTCGCCCAGCTACAGCCGCC ACAGCTCCTACGAGCGGGGCGGCGACGTATCCCCCAGCCCGTACAGCAGCAGCAGCTGGCGGCGCTCGCG CAGTCCCTACAGTCCAGTACTCAGACGATCTGCCAAATCCCGAAGCAGAAGCCCCTATTCATCTAGGCAC TCAAGATCTCGTAGCAGGCACAGATTGTCTAGATCCCGAAGTCGTCATTCAAGCATTTCTCCTAGCACAC TAACTCTGAAGAGTAGCCTGGCAGCTGAATTAAACAAGAATAAAAAGGCACGAGCTGCAGAGGCAGCAAG AGCTGCAGAGGCAGCCAAAGCTGCAGAAGCTGCTAAGGCTGCTGAGGCAGCTGCCAAAGCTGCAAAAGCC TCTAGTGTTTCTACACCTACCAAGGGGAACACAGAAACTGGTGCCAGTGTCTCACAGACAAACCATGTGA AGGAAGTCAAAAAACTTAAAACTGAGCATGCACCTTCTCCTTCAAGTGGTGGGACTGTCAAAAGTGACAA GACAAAAACAAAGCCACCGCTTCAAGTAACAAAAGTAGACAATAATTTGATTGTAGAGAAAGCTACCAAG AAAACAGTTGTTGGGAAAGAGAGTAAACCTGCTGCTACAAAGGAAGAACCAGTGTCTACTAAAGAGAAAA CCAAGCCACTCACACCAAGTGTAGGAGCCAAGGAGAAGGAGCAACATGTGGCTTTAGTGACCTCTACATT ACCACCATTACCTTTGCCTCCCATGTTGCCTGAAGATAAAGATGCTGATAGTTTAAGAGGCAATATTTCT GTAAAAGCAGTTAAAAAAGAAGTAGAAAAGAAACTCCGGTGTCTGCTTGCTGATTTACCATTGCCCCCTG AGTTACCAGGAGGAGATGATCTTTCCAAGAGTCCAGAGGAGAAGAAAACAGCAACACAGTTACATAGCAA AAGAAGGCCTAAAATATGTGGGCCTCGCTATGGTGAAATCAAAGAAAAAGATATTGACTGGGGGAAACGC TGCGTGGATAAATTTGATATCATCGGAATTATTGGAGAAGGTACTTATGGACAAGTTTACAAAGCCAGGG ACAAAGACACTGGAGAAATGGTAGCCTTAAAGAAAGTACGTCTGGATAATGAAAAGGAGGGTTTCCCAAT TACAGCAATTAGAGAAATTAAAATTCTTCGGCAACTTACCCATCAGAGTATCATCAATATGAAGGAAATA GTGACTGATAAAGAAGATGCTTTGGATTTTAAGAAAGACAAAGGTGCATTTTACCTGGTGTTTGAATATA TGGACCATGATCTGATGGGACTGCTGGAGTCAGGCTTGGTTCATTTTAATGAAAACCATATAAAATCTTT TATGAGACAGCTCATGGAAGGCCTGGATTATTGTCATAAGAAGAACTTTTTGCACAGAGATATTAAATGT TCAAATATCCTTCTAAATAATAGAGGACAGATAAAACTTGCAGATTTTGGACTTGCTCGTTTATATAGCT CAGAAGAAAGCCGTCCATATACTAACAAGGTCATTACTTTGTGGTATCGTCCACCTGAACTGCTCTTGGG AGAAGAACGATATACACCGGCCATTGATGTATGGAGCTGTGGATGTATCCTTGGGGAACTCTTCACTAAA AAGCCTATATTTCAAGCAAACCAGGAACTTGCACAACTAGAGCTAATAAGTCGTATATGTGGGAGTCCGT GTCCTGCAGTATGGCCTGATGTAATCAAACTGCCATATTTCAACACCATGAAACCAAAGAAGCAATATCG GCGGAAGTTAAGAGAAGAGTTTGTTTTTATCCCTGCAGCTGCACTCGACTTATTTGATTACATGCTTGCC TTGGATCCCAGTAAGCGCTGCACTGCCGAGCAGGCTCTTCAGTGTGAGTTCCTGCGAGACGTGGAACCCT CCAAAATGCCTCCACCAGACCTTCCTTTGTGGCAAGATTGTCATGAATTATGGAGTAAAAAGAGAAGAAG ACAGAAACAGATGGGCATGACTGATGATCTTTCCACAATCAAAGCCCCCAGAAAGGACTTGTCTCTGGGC TTAGATGACAGCAGAACTAGCACGCCCCAGGGTGTGCTGCCACCTGCACAGCTAAAGTCTCAGAACAGCT CCAGTGGAGCACCTGGTGAAAAACAGACAGATCCATCAACACCACAACAGGAGTCTTCAAAATCATTGGG AGGAGTTCAGCCTTCACAGAGCATCCAGCCTAAAGTGGAAACTGACGCTGCGCAGGCTGCAGTGCAGAGT GCATTTGCAGTCCTCCTGACTCAGTTAATAAAGGCCCAGCAGTCAAAACAGAAAGATGCCATGCTGGAGG AGAGGGAGAATGGATCAGGACATGACGCTCCAGTGCAACTCAGGCCGCCTCCAGAACCCAGCACTCCTGC CTCTGGGCAAGATGACCTCATCCAACACCAAGACAGGAGGATATTGGAGCTGACACCAGAGCCAGAGCGG CCTCGGATTCTGCCTCCTGATCAACGACCTCCTGAACCTCCTGAACCACCACCAGTCACTGAGGAGGACC TGGATTATCGGACAGAAAACCAGCATGGACCTACTACTAGTTCTTCATTAACTGACCCACATGCTGGAGT GAAGGCAGCCCTCTTACAGCTGCTTGCTCAACATCAGCCCCAGGATGATCCCAAAAGAGAAGGTGGTATT GATTATTCCACAGGAGACACGTACGTGCCCAGTTCAGACTATAAAGACAACTTTGGAGCGTCTTTCTCTG CCGCCCCTTATGTGAGCAGTGATGGTCTGGGGAGTAGCTCTGCTGCTGCACCGTTGGAGGCGCGCAGTTT CATCGGAAACTCAGATATTCAGTCTCTGGATAACTACAGTACTGCTTCATCTCACACTGGTGGTCCACCT CAGACTTCTGCCTTCACCGAGTCATTTCCCAGTTCAGTAGCTGGATACGGAGACATTTACCTCAATGCTG GTCCCATGCTGTTTAGTGGAGACAAGGACCATAGATTTGAGTACAGCCATGGTCCTATCACGGTCCTCAC AAACAGCAGTGACCCTTCCACAGGGCCGGAGAGTACTCACCCCTTGCCAGCAAAGATACACAACTATAAC TATGGTGGTAACTTACAGGAAAATCCAGGTGGCCCCAGCCTCATGCATGGACAGACCTGGACTTCTCCTG CCCAAGGACCTGGATACTCACAAGGATACAGGGGACACATTAGCACATCAGCTGGCAGAGGTCGAGGCAG AGGGTTACCATAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217628 representing NM_001271296 Red=Cloning site Green=Tags(s) MPSSSDTALGGGGGLSWAEKKLEERRKRRRFLSPQQPPLLLPLLQPQLLQPPPPPPPLLFLAAPGAAAAA AAAAAASSSCFSPGPPLEVKRLARGKRRPGGRQKRRRGPRAGQEAEKRRVFSLPQPQQDGGGGASSGGGV TPLVEYEDVSSQSEQGLLLGGASAATAATAAGGTGGNGGSPASSSGTQRRAEGSTRRPRRDRRSSSGRSK ERHREHRRRDGTRSGSEASKARSRHGHSGEERAEAAKSGSSSSSGGRRKSASATSSSSSSRKDRDLKAHR SRTKSSKEPPSAYKEPPKAYREDKSEPKAYRRRQRSLSPLGGRDESPVSHRASQSLRSRKSPSPAGGGSS PYSRRLPRSPSPYSRRRSPSYSRHSSYERGGDVSPSPYSSSSWRRSRSPYSPVLRRSAKSRSRSPYSSRH SRSRSRHRLSRSRSRHSSISPSTLTLKSSLAAELNKNKKARAAEAARAAEAAKAAEAAKAAEAAAKAAKA SSVSTPTKGNTETGASVSQTNHVKEVKKLKTEHAPSPSSGGTVKSDKTKTKPPLQVTKVDNNLIVEKATK KTVVGKESKPAATKEEPVSTKEKTKPLTPSVGAKEKEQHVALVTSTLPPLPLPPMLPEDKDADSLRGNIS VKAVKKEVEKKLRCLLADLPLPPELPGGDDLSKSPEEKKTATQLHSKRRPKICGPRYGEIKEKDIDWGKR CVDKFDIIGIIGEGTYGQVYKARDKDTGEMVALKKVRLDNEKEGFPITAIREIKILRQLTHQSIINMKEI VTDKEDALDFKKDKGAFYLVFEYMDHDLMGLLESGLVHFNENHIKSFMRQLMEGLDYCHKKNFLHRDIKC SNILLNNRGQIKLADFGLARLYSSEESRPYTNKVITLWYRPPELLLGEERYTPAIDVWSCGCILGELFTK KPIFQANQELAQLELISRICGSPCPAVWPDVIKLPYFNTMKPKKQYRRKLREEFVFIPAAALDLFDYMLA LDPSKRCTAEQALQCEFLRDVEPSKMPPPDLPLWQDCHELWSKKRRRQKQMGMTDDLSTIKAPRKDLSLG LDDSRTSTPQGVLPPAQLKSQNSSSGAPGEKQTDPSTPQQESSKSLGGVQPSQSIQPKVETDAAQAAVQS AFAVLLTQLIKAQQSKQKDAMLEERENGSGHDAPVQLRPPPEPSTPASGQDDLIQHQDRRILELTPEPER PRILPPDQRPPEPPEPPPVTEEDLDYRTENQHGPTTSSSLTDPHAGVKAALLQLLAQHQPQDDPKREGGI DYSTGDTYVPSSDYKDNFGASFSAAPYVSSDGLGSSSAAAPLEARSFIGNSDIQSLDNYSTASSHTGGPP QTSAFTESFPSSVAGYGDIYLNAGPMLFSGDKDHRFEYSHGPITVLTNSSDPSTGPESTHPLPAKIHNYN YGGNLQENPGGPSLMHGQTWTSPAQGPGYSQGYRGHISTSAGRGRGRGLPY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271296
ORF Size	4353 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271296.1, NP_001258225.1
RefSeq Size	5944 bp
RefSeq ORF	4356 bp
Locus ID	306998
MW	158 kDa

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