Cpamd8 (NM_001271376) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1566313
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217640 representing NM_001271376 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGAAGAACAGAGAGGCTCACCTGTGCCTTTTTTCAGTTCTTCTTGCCCTCCTGCCTTCTGCTTCCT CGTTCAATGGAAACTCGAAGTATATGGTGCTGGTCCCGTCCCAGCTCTACACCGACACCCCTGAGAAAAT CTGCCTGCATCTATACCATCTGAATGAGACAGTGACTGTGACAGCTTCCTTGATATCTCAAAGGGGAACG AGAAAACTGTTCGATGAGCTTGTGGTTGACAAGGACTTGTTCCACTGTCTTTCCTTCACTATCCCTAGAT TGCCCTCTTCTGAAGAGGAGGAGTCACTCGACATCAACATAGAAGGGGCAAAGCATAAATTCAGCAAAAG GCGTGTGGTGCTTGTGAAGAACAAAGAAAGTGTTGTCTTTGTCCAGACTGATAAGCCCGTGTACAAGCCA GGACAGTCAGTTAAATTTCGGATTGTCTCTATGGATAAAAATCTACACCCCCTGAATGAGTTGTTTCCTC TGGCTTACATTGAGGATCCCAAAATCAACCGAATTATGCAGTGGCAGGACATTAAGACAGAGAATGGGCT TAAGCAATTGTCCTTCAGCCTGTCAGCAGAGCCCATTCAGGGCCCCTACAAGATAGTCATTCTCAAACAG TCAGGAGTGAAGGAAGAGCATTCCTTCACCGTCATGGAATTCGTGCTTCCCAGATTTGGTGTCGATGTGA AGGTCCCAAACGCCATCTCTGTGTATGATGAGATAATCAGTGTGACTGCCTGTGCGACATATACCTATGG GAAACCTGTCCCGGGACGTGTAAAGATAAGTCTTTGCCATGGAAATCCTTCCTTTAGATCTCAGACAAAG TCTGCATGCAAAGAAGAGGACTCGGAGCTAGACAACAACGGCTGCAGCACACAAGAAGTGAACATCACTG AGTTCCGATTGAAGGAAAATTACCTCAAAGTGCGCCAGGCTTTCCATGTGAATGCAACTGTTACAGAAGA AGGGACAGGGTCAGAGTTCAGTGGATCTGGAAGAATTGAAGTCAAAAGAACCAGAAACAAATTCCTATTT CTGAAAGCAGATTCCCACTTCAGACATGGGATCCCATTCTTTGTGAAGGTCCGCCTAGTGAATATCAAGG GAGATCCTATCCCAAATCAGCAGGTCTTCATCAAAGCACAAGGAGCTGGCTTCACCAATGCTACTACCAC TGATCAGCATGGCTTGGCCAAGTTCTCCATAGATACCAGCGGCATCTCACGATATATCCTGAATATCAAA GTCTACCACAAGGAGGAAAATTCATGCATCCATTCCTCTTGCACAGCAGAGAGACACGCAGAGGCACATC ATACGGCCTATGCTGTTTACTCCCTCAGCAAGAGCTACATCTACCTTGACACAGAAGCTGGCGTCTTGCC CTGCAACCAGAGCCACACAGTTCAGGCACATTTTATTCTGAAGGGGCAGGTCTTGGGAGTGCTGCCACAG ATTGTTTTCCACTACCTGGTCATGGCCCAGGGCAGCATCCTCCAGACTGGAAACCACACTCATCAAGTGG AGCCAGGAGAATCTCCAGTACAAGGCAACTTCGCCTTGGAGATCCCTGTGGAGTTCAGCATGGTTCCCAT GGCTAAAATGCTCATCTACACCATCTTGCCTGATGGAGAAGTGATTGCAGATTCTGTAAAGTTTCAAGTT GAAAAATGTCTTCGTCATGAAGTGCACCTGAGTTTCAGCCCATCCCAAAGTCTTCCAGCCTCACAAACCC ATATGCGGGTTACAGCTTCTCCTCAGTCCCTCTGTGGCCTGAGAGCTGTGGACCAAAGCGTGCTGCTGCT GAAGCCTGAGGCTGAGCTCTCCCCTTCCTTGATATATGATCTACCAGGTATGCAAGAGAGCAAGTTCATT CCACGTTCCCACTATCCATTTGAAGACGAAGATGGTTGTGTGATATACCGACCAAGAGTCATTGGAAAGC TCACATACTCAGTACCATATGGACGAGAGAAGGATGTCTTCAGATACATGAGGGACACGGGTTTAACGGC ACTCACCAACTTGAAACTTCGTACATTTTGTTTTGACTATGACATGGTTCCATTAGCAGTACCTGCTGCC CGATCTAGTGATTTATTTAACAGCGACGGTTATGGAAGTTGGCGCTCAGCAAGAGTAAAAAGTCTACCTC CTAAAAAGGCACCACATAAAGATCCACCACCTAAAGATCCAGTTATTGAGACCATTAGGAATTATTTTCC TGAAACCTGGGTCTGGGATTTAGTCACAGTAAACTCCTCAGGAGCAGCTGAATTGGAAATGACAGTCCCT GACACCATCACTGAATGGAAGGCCGGAGCCCTCTGCCTGTCCAATGACACTGGTCTTGGCCTCTCTTCTG TGGCAACTCTCCAAGCCTTCCAACCTTTCTTTGTGGAGCTCACAATGCCCTACTCTGTGATCCGTGGAGA AGCCTTCACGCTCAAGGCCACTGTGATGAACTACCTCCCTACAAGCTTGCCGATGGCAGTGCAGCTGGAA GCTTCTCCTGATTTCACAGCTGTCCCAGTGGGAAACGACCAAGATTCTTACTGCCTTGGTGCCAATGGAA GGCACACCTCATCCTGGTTGGTAACTCCCAAATCTTTAGGGAATGTGAATTTCTCTGTGTCTGCGGAAGC ACAACAGTCCCCAGAGCCTTGTGGTTCTGAGGTGGCCACAGTGCCTGAAACCGGGAGAAAGGACACAGTC GTCAAAGTCTTGATAGTTGAGCCTGAAGGAATCAAGAGAGAGCATACTTTCAGCTCACTGCTCTGTGCAT CAGATGCTGAGCTATCAGAAACATTGTCTCTGCTGCTTCCACCAACAGTAGTGAAAGATTCAGCCAGAGC ACATTTCTCTGTGATGGGTGATATCTTGAGTTCAGCCATAAAAAACACACAAAATCTTATCCAAATGCCC TATGGCTGTGGGGAGCAGAACATGGTCCTTTTTGCTCCGAGCATCTATGTACTGAAATATCTGAATGAAA CCCAACAGCTGACAGAGAAGATCAAGTCCAAGGCCCTTGGCTACCTCAGAGCTGGTTATCAGAGGGAACT GAACTACAAACACAAGGATGGCTCCTACAGTGCCTTTGGGGATCACGATGGCAAGGGTCAAGGAAACACT TGGCTCACAGCCTTTGTGCTCAAGTCTTTTTCCCAAGCTCAAGCCTTCATCTTCATTGACGAATCACACA TCACCGATGCCTTCACCTGGCTCTCCCATCAGCAGAAGGACAGTGGCTGCTTCAGGAGCTCTGGATCACT GTTCAACAACGCCATGAAGGGGGGAGTTGATGATGAAATCACCCTCTCTGCCTATATAACTGTGGCCCTT TTGGAAAGTTTACCCCCAGACACTCATCCTGTTGTCTCCAAAGCCCTGAGCTGCCTAGAGTCATCTTGGG AGAACATAGAGCAAGGAGGAAATGGCAGCTTCGTGTACACCAAGGCACTGATGGCCTATGCTTTTGCTCT GGCGGGGAATCAGGAGAAGAGAAACGAAATCCTGAAATCCCTTGATAAGGAAGCTATAAAGGAAGACAAC TCCATTCACTGGGAAAGACCTCAGAAACCCATGAAATCAGCGCGTTCTCTGTACAAACCCCAAGCTTCCT CTGCTGAAGTAGAGATGAATGCCTATGTCATCTTAGCTCGCCTCACTGCCCAGTCAGCCCCATCCCCTAA GGACCTGGCTTTGTCAATGGGCACCATCAAGTGGCTCACAAAGCAGCAGAATTCCCGTGGTGGCTTCTCC TCCACACAGGACACTGTGGTGGCCCTCGATGCTCTGTCCAAATACGGAGCAGCTACTTTTTCCAAAAGCC AAAAAACTCCTTCGGTGACCGTCCAATCTTCAGGGTCATTTTTCCAAAAGTTCCAAGTGGACAACAGTAA TCGTCTGTTACTGCAGCAGGTCTCATTACCAGACATTCCTGGGAACTACACCGTCAGTGTGTCAGGAGAA GGATGTGTGTATGCTCAGACCATGCTGAGATACAACCTGCCCTTGGAGAAGCAGCAGCCTGCGTTTGCTC TAAAGGTGCAGACAGTACCCCTGACTTGTAACAACCCCAAAGGCCAGAACAGCTTCCAAATCTCACTAGA AATCAGTTACACGGGGAGCCGTCCAGCCTCCAACATGGTGATTGCTGATGTGAAGATGCTCTCTGGTTTC ATCCCATTGAAACCAACAGTGAAGAAGCTTGAAAGATTAGAGCATGTAAGCAGAACAGAAGTGACCACCA ACAACGTCCTGTTATATTTGGACCAGGTAACCAATCAGACACTGTCCTTCTCCTTCATCATTCAACAAGA CATCCCAGTAAAGAACCTGCAGCCTGCCATTGTGAAAGTGTATGACTACTATGAGACAGATGAAGTGGCT TTTGCTGAATACAGCAGCCCCTGCAGCTCAGACAACCAAAATGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217640 representing NM_001271376 Red=Cloning site Green=Tags(s) MWKNREAHLCLFSVLLALLPSASSFNGNSKYMVLVPSQLYTDTPEKICLHLYHLNETVTVTASLISQRGT RKLFDELVVDKDLFHCLSFTIPRLPSSEEEESLDINIEGAKHKFSKRRVVLVKNKESVVFVQTDKPVYKP GQSVKFRIVSMDKNLHPLNELFPLAYIEDPKINRIMQWQDIKTENGLKQLSFSLSAEPIQGPYKIVILKQ SGVKEEHSFTVMEFVLPRFGVDVKVPNAISVYDEIISVTACATYTYGKPVPGRVKISLCHGNPSFRSQTK SACKEEDSELDNNGCSTQEVNITEFRLKENYLKVRQAFHVNATVTEEGTGSEFSGSGRIEVKRTRNKFLF LKADSHFRHGIPFFVKVRLVNIKGDPIPNQQVFIKAQGAGFTNATTTDQHGLAKFSIDTSGISRYILNIK VYHKEENSCIHSSCTAERHAEAHHTAYAVYSLSKSYIYLDTEAGVLPCNQSHTVQAHFILKGQVLGVLPQ IVFHYLVMAQGSILQTGNHTHQVEPGESPVQGNFALEIPVEFSMVPMAKMLIYTILPDGEVIADSVKFQV EKCLRHEVHLSFSPSQSLPASQTHMRVTASPQSLCGLRAVDQSVLLLKPEAELSPSLIYDLPGMQESKFI PRSHYPFEDEDGCVIYRPRVIGKLTYSVPYGREKDVFRYMRDTGLTALTNLKLRTFCFDYDMVPLAVPAA RSSDLFNSDGYGSWRSARVKSLPPKKAPHKDPPPKDPVIETIRNYFPETWVWDLVTVNSSGAAELEMTVP DTITEWKAGALCLSNDTGLGLSSVATLQAFQPFFVELTMPYSVIRGEAFTLKATVMNYLPTSLPMAVQLE ASPDFTAVPVGNDQDSYCLGANGRHTSSWLVTPKSLGNVNFSVSAEAQQSPEPCGSEVATVPETGRKDTV VKVLIVEPEGIKREHTFSSLLCASDAELSETLSLLLPPTVVKDSARAHFSVMGDILSSAIKNTQNLIQMP YGCGEQNMVLFAPSIYVLKYLNETQQLTEKIKSKALGYLRAGYQRELNYKHKDGSYSAFGDHDGKGQGNT WLTAFVLKSFSQAQAFIFIDESHITDAFTWLSHQQKDSGCFRSSGSLFNNAMKGGVDDEITLSAYITVAL LESLPPDTHPVVSKALSCLESSWENIEQGGNGSFVYTKALMAYAFALAGNQEKRNEILKSLDKEAIKEDN SIHWERPQKPMKSARSLYKPQASSAEVEMNAYVILARLTAQSAPSPKDLALSMGTIKWLTKQQNSRGGFS STQDTVVALDALSKYGAATFSKSQKTPSVTVQSSGSFFQKFQVDNSNRLLLQQVSLPDIPGNYTVSVSGE GCVYAQTMLRYNLPLEKQQPAFALKVQTVPLTCNNPKGQNSFQISLEISYTGSRPASNMVIADVKMLSGF IPLKPTVKKLERLEHVSRTEVTTNNVLLYLDQVTNQTLSFSFIIQQDIPVKNLQPAIVKVYDYYETDEVA FAEYSSPCSSDNQNV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271376
ORF Size	4455 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271376.1, NP_001258305.1
RefSeq Size	4586 bp
RefSeq ORF	4458 bp
Locus ID	297572
MW	164.9 kDa

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