Ahctf1 (NM_001271270) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ELYS; RGD1561291
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217756 representing NM_001271270 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAGACTTGACAGCTCAAGTGACTAGTGATCTCCTGCAGTTCCCAGAAGTGACTATTGAAGCTCTTG GAGAAGATGAAATAACGTTAGAATCTGTGCTTCGTGGGAAATTTGCTGCCGGGAAAAATGGATTGGCTTG CTTAGCTTGTGGTCCACAACTTGAAGTCGTAAACTCCCTAACAGGAGAGCGGCTGTCTGCATATAGATTC AGTGGAGTAAATGAACAGCCTCCTGTAGTTCTTGCAGTGAAAGAATTCTCTTGGCATAAGAGAACTGGAT TGCTAATAGGATTAGAAGAAGCAGATGGGAGTGTTCTTTGCCTTTATGACCTTGGTATATCCAGAGTGGT CAAAGCAGTTGTTCTTCCTGGAAGGGTAACAGCTATCGAGCCTATAATTAACCATGGAGGAGCCAGTGCA AGTACCCAGCACTTACACCCAAGCCTTCGATGGCTTTTTGGTGTGGCAGCTGTGGTAACTGATGTTGGAC AGATCCTTCTTATTGATCTCTGTTTGGATGACTTGTCATGCAGTCAAAATGAAGTAGAGGCATCAGATCT TGAAGTTATCACTGGTATTCCAGCTGAAGTACCACACATCAGAGAGAGTGTGATGAGAGAGGGGCGCCAC CTGTGTTTCCAGTTAGTTAGCCCATCGGGAGTAGCCATTTCTACCCTCAGTTACATTAACAGGACAAATC AGCTTGCTGTCGGTTTTGCTGACGGCTACTTGGCACTTTGGAACATGAAAAGCATGAAAAGAGAATACTA TACACAATTGGAAGGTGGAAGGGTTCCTGTCTATGCAGTTGTTTTTCAAGAGCCTGAGAATGATCCTCGT AACTGCTGTTATTTATGGGCTGTGCAATCCACACAAGAGAGTGAAGGGGATGTTGTGAGTTTGCATCTGC TTCAGCTGGCTTTTGGTGATAGAAAATGTTTGGCATCAGGGCAGATCTTATATGAGGGATTAGAATACTG CGAAGAAAGATATACACTGGATCTATCAGGTGGCATGTTCCCCTTAAGGGGACAGACTAGCAATACCAAA TTGTTGGGCTGCCAGAGTATAGAGCGATTTCCGTCTCATGGTGACAGAGAAGAAAGCATGAGAGAAGCTC TGTCCCCTGACACTAGCGTTTCTGTCTTTACCTGGCAAGTGAACATATATGGACAGGGAGAGCCTTCTGT GTATTTAGGGCTTTTTGACATAAATCGGTGGTATCATGCACAAATGCCTGATTCTTTAAGGTCTGGGGAA TCTCTGCATAATTGCTCTTACTTCGCATTGTGGTCATTGGAGTCGGTTGTGAGTAGGACTTCTCCACATC ACATCCTGGACATACTCGTACACGAGAGAAGTTTAAGTCGAGGGGTTCCTCCCTCCTATCCACCTCCAGA ACAGTTTTTTAACCCAAGTACTTTTAACTTTGATGCGACTTGCTTGTTAAACTCTGGAGTTATCCATGTA ACTTGTACTGGATTTCAGAAGGAGACTTTGACATTTTTAAAGAAATCAGGACCAACTCTTAATGAAGTCA TTCCCGATAGTTACAATCGATGTCTTGTTGCCGGTCTCCTCTCACCAAGGCTTATTGATATTCAGCCTTC CAGTTTAAGTCAAGAAGAACAATTAGAAGCTATATTGTCAGCAGCAATTCAGACAAGTTCCTTGGGACTT TTGACTGGTTACATCAGAACATGGATAATAGAAGAACAACCAAATTCTGCTGCTAATCTACGGTTTGTTC TTGAGTGGACATGGAATAAGGTGATCCTCACAAAAGAAGACTTTGACAGGCTTTGTGTGCCGTTGTTTGA CGGTTCCTGTCGCTTCATCGACCCACAGACCATTCAGTCCATCCAGCAGTGTCATTTACTGCTTAGCAAC CTCAGTACAGTCTTAAGCTGTTTCGCAATGGAGGCCCAGGGTATCACTGAAAGAGGACTTGTGGACTTGA ACAATAAGCATATGGTTACCCAGCTTATCTGTCAGTATGCACAAATGGTTCTGTGGTTCTGTCACTCTGG GCTTTTACCTGAAGGCTTAGATGATACTCTGCAGCTGTCAAGACTACGCTACAACTACCCTGTAATTCAG AACTACTATACCAGTCGTCGGCAGAAGTGTGAACGTTCACCCAGAGGGAAGTGGAGCCGAGACTGCTTGC TGATCGATGGACTGGTCTCTCAACTAGGAGATGAAGTTGAGAAGCTGTGGAAGCGGGATGAAGGTGGCAC AGGAAAATACCCTCCTGCTAGCATCCATGCATTACTTGATATATATTTATTAGACAACATTAGTGAAGCA AGCAAACATGCTATTACCATTTATTTGCTGCTTGATATTATGTATTCCTTTCCCAATAAAACGGATACCC CCATTGAATCTTTCCCAACTGCTTTTGCTATTTCTTGGGGCCAAGTTAAACTAGTTCAAGGATTTTGGCT GCTAGATCATAATGATTATGAGAATGGTTTAGACCTTCTGTTTCACCCAGTTACTGCAAAACCTGCATCG TGGCAGCATTCAAAGATAATTGAAGCCTTTATGAGTCAGGGAGAGCACAAACAGGCTCTCCGCTATCTTC AGACAATGAAGCCAACAGTGTCCAGTAGCAGTGATGTTCTCCTTCACCTCTCTGTTCTGCTTTTTAATAG ATGCATGGTTGAGGCGTGGAACTTACTGCGACAGAACTCAAACACAGTAAATATAGAGGAATTGCTGAAG CATGCTTATGAAGTTTGTCAGGAGATGGGCTTAATGGAGGATTTACTGAAGCTACCGTTTACAAACACTG AGCAGGAATGTTTAGTGAATTTTTTACAATCCAGTTCCAGTGTTCAGAATCATGAATTCCTTCTAGTTCA CCATTTACAGCGTGCCAATTATATTTCTGCCTTGAAACTGAACCAGACTCTGAAGAATAATCTCACGAGT GATCGTGACCCTCGATTGCGGGAGAGATCAGTGACTCGAAATTCTATATTAGACCAGTATGGGAAAATCC TACCTAGAGTCCAAAGAAAGTTAGCTGTTGAGCGAGCCAAGCCTTACCATCTGTCAACATCGTCAGTTTT TCAGGAAGTTTCTAGACCCAAACCATTATCAGCATTTCCAAAGAAAGCTCTAACTGGAACAGTGATAACG CGGTCTACATTCATCAGCAACGTGCTATCTAAAATTGGAGAGGTGTGGGCAAGCCATGAGCCCAGAAACG GCATCTCACTTTTCAACAGTCCTAAAACAGAGCAACCATCTCCTGTAGTGCACTCTTTCCCACATCCAGA GCTTCCTGAGGCATTTGTTGGAACACCAATTTCAAATCCATCCCAGAGAATTTCTAGATTACTGGATTTG GTTGTTCATCCTATCCCCCAACCCTCCCAACGTTTGGAGTTTATTCAGCAAAGTCCCACAAGATCTCCTT TGTGTCTGCTGTCCAGTTCATTACCATTAAGTTCACAGTTAAAAAGGCCACATCAGCAAGCCTCCAGGCC TTCAGAATTACTTTTACTTGAAACTCCTCTTATAGTTAAGAAAGCTAAGTCTTTGGCTCTCTCAGCTACA TCTTCTGGATTTGCCGAGTTTACTCCTCCATCCATCCTTAGGTCTGGTCTTCGAACAACATCTTTAGCTT CTCCCTCTCAGTCACCTGGAAGATCTCTCACTCCGCCTTTCAGACTTAAAGAAACTAGGATTTCATTCAT GGAAGAAGGCATGAACACACACTGGACTGATACAGCCACAGATGACCGAAATGCAAAAGCGTTTGTTAGT ACATCTTTCCATAAATGTGGACTTCCAGCAGAAACTGAATGGATGAAGACCAATGATAAAAATACATATT TTCCTTTGGATGTCCCTGCAAAGGTTCCTCAGAAAGTGGTTGCAGAATCGCTGGATACCCATTCTCCTCG AAGTCTAGAGAAACTGGATGTGAGCAAAGACAGCACTGTTTCCAGCAGGTCAGACCAGACCTTAGAGTAT CATGATGCACCATCACCAGAGGACTTGGAAGGGGGTGTTTTTGTGTCCCCCAAGCCAGCGAGCTCTTCCA CTGAACTAACTACTAATTCAATGGTACAAACCGAGAAGGATAATGATAAAAATTTGTTTAAGTCAGAAGG TACTCCTTCAGCCATGGAGAAACAGATAGGCACCATAGAAGCTTCGGTGGAAGCATTTTCAGAATTCAGT CACTTAGACCCTGTTGAAAGAGCTGAAGCTTCTCTTGGTGTGTCATCTGTCCGTGAAGGGGAAACCTCCA CTTCAAAATCCAAGACATCGGTTCTGGATGGAATAGTGTCTATTGAGAGCCGAACTTCCATACCTATAGC AGACCACAATGAATTTGTGGCCAACAAGGTAGAAGATGTTGAAAGCTCTGAGTCTACCACCTCCAAGTGC CCTGTTACCTCTGAACGTAGCCTTGACAAAGAACTAACATTAAATTTAAAAGGAGATGAAATGGAAGCTG TTGTACCAAAAGAGAGCGTTGGCTTACCAGAAGAAAGACCTCAGACTTCTGCTGGTCCTTCTGATACTCA AGAAATTCATCTAATTGGATGTGAAAAACTTGAAGTTCAAAATTCAGAAGAGGTGGCCAAGAGTCTTTCG TTTCCCGAGTTGTGTCCCTTAGGAGCACACAGACCTCAGTATAATTTCGATGTCATTGAGCAGCAGTTTC GTGACTTGCCCGATGATAAAGACTCTGCTGAATGTGATGTCGCTGAAGTGGATGGGGAGCTCTTTGTGGC CCAGAGCAACTTTAGCTTGATTTTAGAAGGCGAAGAAGGAGAGGTTGAGGCAAGTGACTCTAATGTGTTA CCGAAAGTGACCAACATAGCAACCAAGGAAAAACCTGTGTCCTGCGAGGAACCTGATAATGAAGGACATG TTACAGATTTGCCATCTGCTGTGACTGGTGACCAAGAGTCTCACAAGGTAGAGACTTTACCATATGTGCC TGAACCAGTTAAAGTGGCAATTGCAGAAAATTTGTTGGATGTAATTAAAGACACACGAAGTAAAGAAGCA ACTCACGTGGCAGCCGTTGAGGATGTCGATGAGGACAGAGCAGTAATGATCCCCAAGGCTGCACATCCGT CCAGGCTGACGAACTCCACACCGAAGACTGTTAAGGAAAGACGCGCCGAGACCGTAAGTGCCAGCCAGGG TGACGACAGCATAGCTCCTAGAACTCTCACAAGAAGGCAGCGTGCCCTAAGCCTGAATGTCACTTCAGAA CAAGAGCCCTCAGCAGCTGCTACTCCTCAGAAGAAAACTAGAAAAGTAAAAGAAACTCGTGAGTTTTCTG AAAGTACCTGTTCTGACGTAAAAGTAGCACCTGAGAACCAGCTGACAGCTCCCAGTCCTTCCCCTTCCAG GAGAGGAAGGAAGAAAAAGGACCTTGGCCAAGGTCCACTGGCAACCTCTGGTGCTGTGGAGGAGCCCCAG GCGACACCAGAAAGACTGAGGCTGAGAGTGCAGCCACCTGAGCCAGCAGCTGAGGAAACACCTTCTAGAG CAAAAGCCAAGCTTTCATCTGCTAGGAAGGGGACTCCTAGAAGACTTAGGAAATCTATAGAAGATGGGCC AAGTGCAGACATTGTAAATGAGGTCAGAGGCAGGAAAGCAGCCGGTCATGGCAGGACTGTCAGAGAGCTG AGGAGTGCCAGTGTAGAGGATACTCAAAAGATGGAGTATGAACAAGATGAGGAACGCGGTGACCAGCAAC TGCCTCTAAAACGAAAAAGGGTCAGAGAGAGAGAAGATAGTGTGTCCGGTGTGACAGAAGAGCCTAAGCT TGACTCATCCCAGTTAACTCTTCAGGTGGGACTTGATGTACCTGCCACCCCTAGGAAACGTGGTAGAAGG CCCAAGAGGGTAGTTCCCTTAGGAGCTGATGGTACCACACCAGGTAAGGAACAGACAAGCCCCCAGAAGG AAGGTCCAGTTGTCCGGAGGTCTACACGGAACACCCCAGCTAGAAATGTGAGTACTTTAGAAAAACCCAT TTTAGTGCCAAATAAGGAAGCTGCTACAGTGATGACGTCTAAGAAAAAACCTACGAAGAAGTCTGCAGAT GGAAGCCCAAAAGGTCCATTTCCAGCAGTATCAGACTTGGCGGATGAAGCAGCCCACCGAGAGTCCACTG ACCAAGGAGTGCTCACCAGTGCTGCGTTCACTACGCCCTCCTGGAGCACAAGGACTAGGTCTAGAAGGAC CATGTTGTTAACGGACATTTCTGAGCCCCAAACTGAGCCTTTGCTTTCCTCCCCTTCATTGAAGGTGCCA AAGAAAGAGACAAAAGCTAAAAACATGGAGGCCGCTGCCCAGCTGAAAGAATTGGTGTCAGATATATCTT CTCGGTTTGTGGTTTCCCCTCCTGCCTTGAGAACCAGGCAGAAAAGTACATCCAATGCTTCCAAGCTTCT AGGTGAATTGGAGAGTAACCCTAAACCAGTAGAAATCATAGAACAAAAACCAAAAAGAAGCAGGACTGTG AAGACAAAAGTGAGCAGAAACACAGGAAAAGGAAGTTCTTGGTCACCTCCCCCTATAGAAATTAAACTGA TCTCTCCCTTGGCAAGTCCGGTGGATGAAATAAAGGTCAGCAAGCCAAGAAAGACTGCAGAAGTAGCAGG AAAAACTCTTGGAAGGGGCAGAAAGAAGCCATCTTCTTTTCCAAAGCAAATTTTACGCAGGAAAATGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217756 representing NM_001271270 Red=Cloning site Green=Tags(s) MQDLTAQVTSDLLQFPEVTIEALGEDEITLESVLRGKFAAGKNGLACLACGPQLEVVNSLTGERLSAYRF SGVNEQPPVVLAVKEFSWHKRTGLLIGLEEADGSVLCLYDLGISRVVKAVVLPGRVTAIEPIINHGGASA STQHLHPSLRWLFGVAAVVTDVGQILLIDLCLDDLSCSQNEVEASDLEVITGIPAEVPHIRESVMREGRH LCFQLVSPSGVAISTLSYINRTNQLAVGFADGYLALWNMKSMKREYYTQLEGGRVPVYAVVFQEPENDPR NCCYLWAVQSTQESEGDVVSLHLLQLAFGDRKCLASGQILYEGLEYCEERYTLDLSGGMFPLRGQTSNTK LLGCQSIERFPSHGDREESMREALSPDTSVSVFTWQVNIYGQGEPSVYLGLFDINRWYHAQMPDSLRSGE SLHNCSYFALWSLESVVSRTSPHHILDILVHERSLSRGVPPSYPPPEQFFNPSTFNFDATCLLNSGVIHV TCTGFQKETLTFLKKSGPTLNEVIPDSYNRCLVAGLLSPRLIDIQPSSLSQEEQLEAILSAAIQTSSLGL LTGYIRTWIIEEQPNSAANLRFVLEWTWNKVILTKEDFDRLCVPLFDGSCRFIDPQTIQSIQQCHLLLSN LSTVLSCFAMEAQGITERGLVDLNNKHMVTQLICQYAQMVLWFCHSGLLPEGLDDTLQLSRLRYNYPVIQ NYYTSRRQKCERSPRGKWSRDCLLIDGLVSQLGDEVEKLWKRDEGGTGKYPPASIHALLDIYLLDNISEA SKHAITIYLLLDIMYSFPNKTDTPIESFPTAFAISWGQVKLVQGFWLLDHNDYENGLDLLFHPVTAKPAS WQHSKIIEAFMSQGEHKQALRYLQTMKPTVSSSSDVLLHLSVLLFNRCMVEAWNLLRQNSNTVNIEELLK HAYEVCQEMGLMEDLLKLPFTNTEQECLVNFLQSSSSVQNHEFLLVHHLQRANYISALKLNQTLKNNLTS DRDPRLRERSVTRNSILDQYGKILPRVQRKLAVERAKPYHLSTSSVFQEVSRPKPLSAFPKKALTGTVIT RSTFISNVLSKIGEVWASHEPRNGISLFNSPKTEQPSPVVHSFPHPELPEAFVGTPISNPSQRISRLLDL VVHPIPQPSQRLEFIQQSPTRSPLCLLSSSLPLSSQLKRPHQQASRPSELLLLETPLIVKKAKSLALSAT SSGFAEFTPPSILRSGLRTTSLASPSQSPGRSLTPPFRLKETRISFMEEGMNTHWTDTATDDRNAKAFVS TSFHKCGLPAETEWMKTNDKNTYFPLDVPAKVPQKVVAESLDTHSPRSLEKLDVSKDSTVSSRSDQTLEY HDAPSPEDLEGGVFVSPKPASSSTELTTNSMVQTEKDNDKNLFKSEGTPSAMEKQIGTIEASVEAFSEFS HLDPVERAEASLGVSSVREGETSTSKSKTSVLDGIVSIESRTSIPIADHNEFVANKVEDVESSESTTSKC PVTSERSLDKELTLNLKGDEMEAVVPKESVGLPEERPQTSAGPSDTQEIHLIGCEKLEVQNSEEVAKSLS FPELCPLGAHRPQYNFDVIEQQFRDLPDDKDSAECDVAEVDGELFVAQSNFSLILEGEEGEVEASDSNVL PKVTNIATKEKPVSCEEPDNEGHVTDLPSAVTGDQESHKVETLPYVPEPVKVAIAENLLDVIKDTRSKEA THVAAVEDVDEDRAVMIPKAAHPSRLTNSTPKTVKERRAETVSASQGDDSIAPRTLTRRQRALSLNVTSE QEPSAAATPQKKTRKVKETREFSESTCSDVKVAPENQLTAPSPSPSRRGRKKKDLGQGPLATSGAVEEPQ ATPERLRLRVQPPEPAAEETPSRAKAKLSSARKGTPRRLRKSIEDGPSADIVNEVRGRKAAGHGRTVREL RSASVEDTQKMEYEQDEERGDQQLPLKRKRVREREDSVSGVTEEPKLDSSQLTLQVGLDVPATPRKRGRR PKRVVPLGADGTTPGKEQTSPQKEGPVVRRSTRNTPARNVSTLEKPILVPNKEAATVMTSKKKPTKKSAD GSPKGPFPAVSDLADEAAHRESTDQGVLTSAAFTTPSWSTRTRSRRTMLLTDISEPQTEPLLSSPSLKVP KKETKAKNMEAAAQLKELVSDISSRFVVSPPALRTRQKSTSNASKLLGELESNPKPVEIIEQKPKRSRTV KTKVSRNTGKGSSWSPPPIEIKLISPLASPVDEIKVSKPRKTAEVAGKTLGRGRKKPSSFPKQILRRKML TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271270
ORF Size	6720 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271270.1, NP_001258199.1
RefSeq Size	8489 bp
RefSeq ORF	6723 bp
Locus ID	360886
MW	247.9 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.