DNAJC10 (NM_018981) Human Untagged Clone
CAT#: SC100719
DNAJC10 (untagged)-Human DnaJ (Hsp40) homolog, subfamily C, member 10 (DNAJC10)
CNY 8,720.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | ERdj5; JPDI; MTHr; PDIA19 |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC100719 sequence for NM_018981 edited (data generated by NextGen Sequencing)
ATGGGAGTCTGGTTAAATAAAGATGACTATATCAGAGACTTGAAAAGGATCATTCTCTGT TTTCTGATAGTGTATATGGCCATTTTAGTGGGCACAGATCAGGATTTTTACAGTTTACTT GGAGTGTCCAAAACTGCAAGCAGTAGAGAAATAAGACAAGCTTTCAAGAAATTGGCATTG AAGTTACATCCTGATAAAAACCCGAATAACCCAAATGCACATGGCGATTTTTTAAAAATA AATAGAGCATATGAAGTACTCAAAGATGAAGATCTACGGAAAAAGTATGACAAATATGGA GAAAAGGGACTTGAGGATAATCAAGGTGGCCAGTATGAAAGCTGGAACTATTATCGTTAT GATTTTGGTATTTATGATGATGATCCTGAAATCATAACATTGGAAAGAAGAGAATTTGAT GCTGCTGTTAATTCTGGAGAACTGTGGTTTGTAAATTTTTACTCCCCAGGCTGTTCACAC TGCCATGATTTAGCTCCCACATGGAGAGACTTTGCTAAAGAAGTGGATGGGTTACTTCGA ATTGGAGCTGTTAACTGTGGTGATGATAGAATGCTTTGCCGAATGAAAGGAGTCAACAGC TATCCCAGTCTCTTCATTTTTCGGTCTGGAATGGCCCCAGTGAAATATCATGGAGACAGA TCAAAGGAGAGTTTAGTGAGTTTTGCAATGCAGCATGTTAGAAGTACAGTGACAGAACTT TGGACAGGAAATTTTGTCAACTCCATACAAACTGCTTTTGCTGCTGGTATTGGCTGGCTG ATCACTTTTTGTTCAAAAGGAGGAGATTGTTTGACTTCACAGACACGACTCAGGCTTAGT GGCATGTTGGATGGTCTTGTTAATGTAGGATGGATGGACTGTGCCACCCAGGATAACCTT TGTAAAAGCTTAGATATTACAACAAGTACTACTGCTTATTTTCCTCCTGGAGCCACTTTA AATAACAAAGAGAAAAACAGTATTTTGTTTCTCAACTCATTGGATGCTAAAGAAATATAT TTGGAAGTAATACATAATCTTCCAGATTTTGAACTACTTTCGGCAAACACACTAGAGGAT CGTTTGGCTCATCATCGGTGGCTGTTATTTTTTCATTTTGGAAAAAATGAAAATTCAAAT GATCCTGAGCTGAAAAAACTAAAAACTCTACTTAAAAATGATCATATTCAAGTTGGCAGG TTTGACTGTTCCTCTGCACCAGACATCTGTAGTAATCTGTATGTTTTTCAGCCGTCTCTA GCAGTATTTAAAGGACAAGGAACCAAAGAATATGAAATTCATCATGGAAAGAAGATTCTA TATGATATACTTGCCTTTGCCAAAGAAAGTGTGAATTCTCATGTTACCACGCTTGGACCT CAAAATTTTCCTGCCAATGACAAAGAACCATGGCTTGTTGATTTCTTTGCCCCCTGGTGT CCACCATGTCGAGCTTTACTACCAGAGTTACGAAGAGCATCAAATCTTCTTTATGGTCAG CTTAAGTTTGGTACACTAGATTGTACAGTTCATGAGGGACTCTGTAACATGTATAACATT CAGGCTTATCCAACAACAGTGGTATTCAACCAGTCCAACATTCATGAGTATGAAGGACAT CACTCTGCTGAACAAATCTTGGAGTTCATAGAGGATCTTATGAATCCTTCAGTGGTCTCC CTTACACCCACCACCTTCAACGAACTAGTTACACAAAGAAAACACAACGAAGTCTGGATG GTTGATTTCTATTCTCCGTGGTGTCATCCTTGCCAAGTCTTAATGCCAGAATGGAAAAGA ATGGCCCGGACATTAACTGGACTGATCAACGTGGGCAGTATAGATTGCCAACAGTATCAT TCTTTTTGTGCCCAGGAAAACGTTCAAAGATACCCTGAGATAAGATTTTTTCCCCCAAAA TCAAATAAAGCTTATCAGTATCACAGTTACAATGGTTGGAATAGGGATGCTTATTCCCTG AGAATCTGGGGTCTAGGATTTTTACCTCAAGTATCCACAGATCTAACACCTCAGACTTTC AGTGAAAAAGTTCTACAAGGGAAAAATCATTGGGTGATTGATTTCTATGCTCCTTGGTGT GGACCTTGCCAGAATTTTGCTCCAGAATTTGAGCTCTTGGCTAGGATGATTAAAGGAAAA GTGAAAGCTGGAAAAGTAGACTGTCAGGCTTATGCTCAGACATGCCAGAAAGCTGGGATC AGGGCCTATCCAACTGTTAAGTTTTATTTCTACGAAAGAGCAAAGAGAAATTTTCAAGAA GAGCAGATAAATACCAGAGATGCAAAAGCAATCGCTGCCTTAATAAGTGAAAAATTGGAA ACTCTCCGAAATCAAGGCAAGAGGAATAAGGATGAACTTTGA Clone variation with respect to NM_018981.1 609 c=>t;756 c=>t;1575 g=>a;1938 t=>g;2241 a=>g >OriGene 5' read for NM_018981 unedited
ACACTATATTTGTAAACGACTTTTATAGGCGGCCGACGAATTCGCACGAGGCCAATCCCG TGCGCCGCGGCTGGGCCGTCGGAGAGTGCGTGTGCTTCTCTCCTGCACGCGGTGCTTGGG CTCGGCCAGGCGGGGTCCGCCGCCAGGGTTTGAGGATGGGGGAGTAGCTACAGGAAGCGA CCCCGCGATGGCAAGGTATATTTTTGTGGAATGAAAAGGAAGTATTAGAAATGAGCTGAA GACCATTCACAGATTAATATTTTTGGGGACAGATTTGTGATGCTTGATTCACCCTTGAAG TAATGTAGACAGAAGTTCTCAAATTTGCATATTACATCAACTGGAACCAGCAGTGAATCT TAATGTTCACTTAAATCAGAACTTGCATAAGAAAGAGAATGGGAGTCTGGTTAAATAAAG ATGACTATATCAGAGACTTGAAAAGGATCATTCTCTGTTTTCTGATAGTGTATATGGCCA TTTTAGTGGGCACAGATCAGGATTTTTACAGTTTACTTGGAGTGTCCAAAACTGCAAGCA GTAGAGAAATAAGACAAGCTTTCAAGAAATTGGCATTGAAGTTACATCCTGATAAAAACC CGAATAACCCAAATGCACATGGCGATTTTTTAAAAATAAATAGAGCATATGAAGTACTCA AAGATGAAGATCTACGGAAAAAGTATGACAAATATGGAGAAAAGGGACTTGAGGATAATC AAGGTGGCCAGTATGAAAGCTGGAACTATTATCGTTATGATTTTGGTATTTATGATGATG ATCCTGANATCATAACATTGGAAAGAAGAGAATTTGATGCTGCTGTTAATTCTGGAGAAC TGTGGGGTTGTAATTTTTACTNNCCCAGCTGNTCACACTGCCATGATTTAGCTCCACATG GAGAGACTTTGCTAAN >OriGene 3' read for NM_018981 unedited
ACTATGTACCGCGCCGCTTTCTANATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTGAAAAATTGCAAGCCTTTTAATTCATTGACCTCTTTTGACTGATTGGAATTTTTACCA TTCTGTACACTCTGAATTATAACTAAACAACTACATGTTACTGACAAATTTGTGATTCTT TGAGTCTAAACAACACAGAAACTAGAAGATCCCTTTCCTAGATTTATATACTTGATTTTC TTATAAAAATAACTTCAGTTATTATGTCAATATTTATCTGAAAAAGGCAGCCCTCTGACA GTAAACTAGTAACTGGAATAACTACAGTATCATTATAAGCTACAGTAAAACAACCTGTAA AGTAATTTTTCCTATTTGAATAATAAATAGACTGGACAGGAGTGAAAAACCAAAACTATG AAAGAAAAACCCAATGTAAATAAAGGATACAGGTCAAAACAAAGTTCAGAAGGCAAATAT GTAAATATTAGATGCCTCAAAAATCAACTATCAGTGGGAAAATTTCTATGAAAGCAATTT CAGAAAATACATGAACACATATATCTTTAAAATATAGAACTCTAAATACTTGATAACATA TTTAGGATACTCTACAATGCCCAGTTAATTGCTATAATTTTTTTCCCTCAGGTTTTCCTG GATGATACAGCAAACTACAGAATTTGTGCACTTACTGTTGCTCAAATTTGTGTACTTACT GTGTGCACATATACTGCTGCACTGTTTCTCGGCTGTGAAAAATTAAAGCATTTTTTCACC TTTGAGAAGGAAGAAAACATGAAGATCTCCTATAATTATACACCTACAGGGTTTGTTACT GTGCCACATCATGGTGTTTTTAAACGAAAATAAATTATGGGTTAGGTAGCATTTTTAGGG ACAATTGACCAAACTAGTTACCTTTTTCGGGTCGCCCAAAACTGGAGGGAAGAGGCTTAT CCN |
Restriction Sites | NotI-NotI |
ACCN | NM_018981 |
Insert Size | 4700 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_018981.1, NP_061854.1 |
RefSeq Size | 4193 bp |
RefSeq ORF | 2382 bp |
Locus ID | 54431 |
UniProt ID | Q8IXB1 |
Domains | DnaJ, thiored |
Protein Families | Druggable Genome, Transmembrane |
Gene Summary | This gene encodes an endoplasmic reticulum co-chaperone which is part of the endoplasmic reticulum-associated degradation complex involved in recognizing and degrading misfolded proteins. The encoded protein reduces incorrect disulfide bonds in misfolded glycoproteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2012] Transcript Variant: This variant (1) encodes the longest isoform (1). |
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Other Versions
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CNY 8,712.00 |
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RC212632L3 | Lenti-ORF clone of DNAJC10 (Myc-DDK-tagged)-Human DnaJ (Hsp40) homolog, subfamily C, member 10 (DNAJC10) |
CNY 11,112.00 |
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RC212632L4 | Lenti-ORF clone of DNAJC10 (mGFP-tagged)-Human DnaJ (Hsp40) homolog, subfamily C, member 10 (DNAJC10) |
CNY 5,890.00 |
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RG212632 | DNAJC10 (tGFP-tagged) - Human DnaJ (Hsp40) homolog, subfamily C, member 10 (DNAJC10) |
CNY 4,370.00 |
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