United States
Germany
Japan
United Kingdom
China
MRPS27 (NM_015084) Human Untagged Clone
CAT＃: SC101669
MRPS27 (untagged)-Human mitochondrial ribosomal protein S27 (MRPS27), nuclear gene encoding mitochondrial protein
CNY 3,656.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MRP-S27; S27mt |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC101669 sequence for NM_015084 edited (data generated by NextGen Sequencing)
ATGGCTGCCTCCATAGTGCGGCGCGGGATGCTCCTGGCGCGGCAAGTGGTTCTTCCTCAG CTCTCTCCTGCAGGTAAAAGATACCTGCTTTCTTCAGCCTATGTAGACAGCCACAAATGG GAAGCAAGAGAAAAAGAACATTACTGTCTTGCTGATCTTGCATCTTTAATGGATAAAACA TTTGAGAGAAAGTTGCCTGTTAGTTCTTTAACAATATCACGGCTTATAGACAACATTTCC TCTCGGGAAGAGATAGATCATGCAGAGTATTACCTTTACAAGTTTCGACACAGCCCCAAC TGCTGGTACCTGAGAAACTGGACTATCCACACCTGGATTAGGCAGTGTCTAAAATATGAT GCACAAGACAAAGCCCTATATACCCTTGTAAATAAGGTTCAATATGGAATTTTTCCAGAT AACTTTACATTCAATTTACTGATGGATTCTTTCATAAAGAAAGAAAATTACAAAGATGCT TTATCTGTGGTTTTTGAGGTCATGATGCAAGAAGCCTTTGAAGTGCCTTCCACCCAACTT CTCTCCCTCTATGTTTTATTTCATTGCCTGGCAAAGAAGACAGACTTCAGTTGGGAAGAG GAGAGGAACTTTGGTGCATCCCTTTTGCTTCCAGGCCTAAAACAAAAGAACTCAGTGGGT TTCAGTTCCCAGTTGTATGGCTATGCACTTCTTGGGAAGGTGGAGTTGCAGCAAGGGCTA CGGGCTGTGTACCACAACATGCCTCTGATATGGAAACCAGGCTACCTTGACAGAGCCCTT CAAGTGATGGAGAAAGTGGCTGCCTCCCCAGAAGACATAAAGCTGTGTAGAGAAGCGCTC GATGTGCTGGGTGCAGTGCTGAAGGCTCTGACTTCAGCTGATGGGGCTTCAGAGGAGCAG TCCCAAAATGATGAAGACAACCAGGGGTCAGAAAAACTGGTGGAGCAGTTAGACATCGAG GAAACAGAGCAGTCCAAGCTTCCTCAATACCTGGAACGATTTAAGGCCTTACATTCTAAG CTTCAAGCTCTGGGCAAAATTGAGTCAGAAGGTCTTTTAAGTCTGACCACCCAGCTTGTC AAGGAAAAACTCTCCACCTGTGAAGCAGAGGACATCGCCACCTATGAGCAGAATCTGCAG CAGTGGCATCTAGACCTTGTACAGTTGATCCAGAGAGAACAGCAACAGAGGGAGCAAGCG AAGCAGGAGTACCAGGCTCAGAAAGCAGCAAAGGCATCTGCCTAA Clone variation with respect to NM_015084.2 >OriGene 5' read for NM_015084 unedited
CGCACGAGGCACTCGTGCCCGAAATCGGCACGAGGCCAAGATGGCTGCCTCCATAGTGCG GCGCGGGATGCTCCTGGCGCGGCAAGTGGTTCTTCCTCAGCTCTCTCCTGCAGGTAAAAG ATCCTGCTTTCTTCAGCCTATGTAGACAGCCACAAATGGGAAGCAAGAGAAAAAGACATT ACTGTCTTGCTGATCTTGCATCTTTAATGGATAAAACATTTGAGAGAAAGTTGCCTGTTA GTTCTTTAACAATATCACGGCTTATAGACAACATTTCCTCTCGGGAAGAGATAGATTCAT GCAGAGTATTACCTTTACAAGTTTCGACACAGCCCCAACTGCTGGTACCTGAGAAACTGG ACTATCCACACCTGGATTAGGCAGTGTCTAAAATATGATGCACAAGACAAAGCCCTATAT ACCCTTGTAAATAAGGTTCAATATGGAATTTTTCCAGATAACTTTACATTCAATTTACTG ATGGATTCTTTCATAAAGAAAGAAAATTACAAAGATGCTTTATCTGTGGTTTTTGAGGTC ATGATGCAAGAAGCCTTTGAAGTGCCTTCCACCCAACTTCTCTCCCTCTATGTTTTATTT CATTGCCTGGCAAAGAAGACAGACTTCAGTTGGGAAGAGGAGAGGAACTTTGGTGCATCC CTTTTGCTTCCAGGCCTAAAACAAAAGAACTCAGTGGGTTTCAGTTCCCAGTTGTATGGC TATGCACTTCTTGGGAAGGNTGNAGTTGCAGCAAGGGCTACNGGCTGTGTACCACAACAT GCCTCTGATATGGAAAACCAGCTACCTTGACAGAGCCTTTCAGTGATGGAGAAAGTGGCT GCCTCCCCAGAAA >OriGene 3' read for NM_015084 unedited
GCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGATCCCGGGACATTTATTTC CAGAGGCTTTTGATAGAAGAGCACAATCTTGAATAAAATTACACCACTGTACTTAAGGGC AACACTACATAGTAAGCAAAATCTGCTCTGAACCCTTTCACAGAAGAGACACTTCAGAAA GGAATTTTACATCTCCTGACAGTTTTACAACATTCATACCTCTGATGCACTTCAAGCCCC AACCCATCTCTCATTTTCTAACTTCTTTAACAGAAATGTGTGTTCACAAGTTGTGTGTGC ATGCACATGTGCCTCTGTGTGTACACAAAAGGCTCATTTTACCTACTTGATAAATGAGCT GAGACGATGTTTTGGGAAAAAAAAAATTGGAAAAAAAATGTTACCCCAACACGATGGAGA AGCCAATCCAAAAAAGCTTCATGCTAGAGCTAGCTTTCTCCTCAGAAACTTACATCCTAA AAACATCCCTATGGGTGCCTAAAAATGGAAATCCAGTGAGTTCATGGCCTTGAGTTCACT CCTGTCCTGAATTTCTCCCAGTGCAGGTCCATAAGGGCGAGATGTGGCAAAAAGGAGAAT GTGGGAATGTGTGAGTATCCCACAGCCAGCTCACAGTGCAGCTTGCACTCAGAAAGGAGA CTCAGGGACACAGGTCTCAGGATGCACAGTTCACCCTTGGCCTCATGTCCCTGGGCTGGG GAGAGGAGACCATTCAAAGCCTGTGCTTGGCCGGGTGTGGGGGCTCACCCTTATATCCCA ACCCCTGGGAAGCTGAGGCGGCCAAACACCTGAGTCAGGAGTTGAGACCACCCTGCCAAC ATGGGAAACCCCTCTTTCTTAAATGCAAAATTAAAAGTGTGGTGGGTGCCCTTGTATCCC CCTCTAAGGAGCCCAGCCGGAAAATGTTTGACTCGGAACCGAGTTGCATGGCCAAATTGC CCCTGCCTCCCCTGGGAAAAAGAAATCGTTCAAACCAAACCAAAACAAC |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_015084 |
| Insert Size | 2710 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015084.1, NP_055899.1 |
| RefSeq Size | 2631 bp |
| RefSeq ORF | 1245 bp |
| Locus ID | 23107 |
| UniProt ID | Q92552 |
| Gene Summary | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that may be a functional partner of the death associated protein 3 (DAP3). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2013] Transcript Variant: This variant (2) lacks an exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter compared to isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209478 | MRPS27 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein S27 (MRPS27), nuclear gene encoding mitochondrial protein |
CNY 3,656.00 |
|
| RC209478L1 | Lenti ORF clone of Human mitochondrial ribosomal protein S27 (MRPS27), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC209478L2 | Lenti ORF clone of Human mitochondrial ribosomal protein S27 (MRPS27), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
| RC209478L3 | Lenti ORF clone of Human mitochondrial ribosomal protein S27 (MRPS27), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209478L4 | Lenti ORF clone of Human mitochondrial ribosomal protein S27 (MRPS27), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
| RG209478 | MRPS27 (tGFP-tagged) - Human mitochondrial ribosomal protein S27 (MRPS27), nuclear gene encoding mitochondrial protein |
CNY 5,256.00 |
|
| SC324057 | MRPS27 (untagged)-Human mitochondrial ribosomal protein S27 (MRPS27), nuclear gene encoding mitochondrial protein |
CNY 3,656.00 |
