MYO5A (AK090859) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GS1; MYH12; MYO5; MYR12
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK090859, the custom clone sequence may differ by one or more nucleotides ATAGTTACTTGCTATAACTCAGCTTGACTTCTGTCATGAATCAGTGCTCTCTGGGAGGATGCAATACTCT GTTTGGGCATTAATTGGTAGCAGGTTGTCTCAACCAAAAAGACAGGAAACAGCAAAAGCCTCTCTGAAAT TAAGAGGAAAGTTACTCTCCCCACACCCATCAGAGTCTTTATTGGAGCCACCAGGTGAGCTGTGCAGCCT GGACAGGCCTGCAGCTATAGGCCACCTTCCCAGTTTAGGTCCTCAGCACAGGGGAGCCCAAGTCACTGGG TGCCTTCTGAGGGCTGTCACTGGGCAGGCCATATACAAATCAGTGTGTGCGTGGGCACTGCAGTGTGTGC ATGCCGTAGGTGTTGATGGGTGCTAGGAGGGGTGTCGTGTGCATGCGCGTTGAAGAGGATCTGTATTGCC GTGACCTCTGTTCATGGATGAGTGCATTGTAATTTGTTCTCAGGCTGTGCTGTGAGGGCCGCCTTAACCC TTGCTCCCTTCCCTTCTAGAGCTGCCTTAAGTTCTCCAGAACTTTTCTTCTGTAAAGGATATCTTGCCTG GAAGGGATATCTTGCCCTGTTTCTCAAGGTTTTGTGAGAGTTTTGACTGGATGTGGCCCTGCATGACCCT CCTTCTCCTGTACTTCCTCTTTCCTTTCCAAATGGGAATTAGAACTGTGGGGCAGCAACAGTCTCAGAGC CAGTGAGAGGCCAGCTTAGAGAATGCTTCTGAGTTAGTGGGACTCTGTGTCACAAGTAAGCAAATGAATA TATGAAAGAAATTATGGAGATAAGTTAGATTCTTGGTAATACTTAAATGTCTTGCTTTCTACTAACCTTT TGTTACTAAAGGTAAAGGGTATAACTCAAACTTTTTGTGGACATTCTTTTCAAAATTTTTTAAGAACCCT GTACTATAAAAGGTTGAGTAAAAACAGGAAAGCGTGCTATAAGTTCAAATCTGTTGTATTACCCTAAATT AGATAAACCAACCTGAATTATAGTAGATTTCTCAATAGATGAGGAACTGAAAAATACTATGTAAAATATC TTCCAAAATGCTTTTTATACTTTTTTTATTTGTAATTTGGTCTATCTAAAATGTTCGTTAGCTTAACTTA ATGGGCGTTATTGGATTCATATGACTAACGTTTCCTCAGTATTGTAATGCTTGAAATATTTGAAAGAAAA AATGTTGTTTTTTAGTTGAAACTGGTATATATAATTCAGTGCTTGGCAGGTTAGTATATTTTTATGCATT TTTCAGAGTCAGCAGTTTCAAATCTTATTGTTATCATGTTATAAAATTTTAGCCCACATTTCAGGCTCCG TAAATCATTTGAGCCATTATTTTTTCCCAACAAATGGTGAATTTTTTCTTTAAATGTGGATATATATGTT GTAATTTATGATTCCTGGTTATGTATTTTTGTGGGATCCTGCAGTAAAATTGACTTTTTTGTGTCTTTGG GAGATTTAAATTGCGCTAACAGTGTTGCGCAAAAATGAGTTCATGCCATTTAACATATTGTATTTTAATT ATTAACTGTATTAATTTACTATGAAATGGACATCCTTTTAACTAAAATGGAATTGAACATTGCAGTTTTC AAATATTTTTCCTTGTTGGGTCTGGAAAAGGAATTCTACTTTGATCTGCATAGAAAATTTTGATACAATT TTTTGAAAGTTCTTAGGTGAAACATTTACCCATTAAAAAGGAAGCAGAAATACTGAGACATGAAAGGCAT TATCAACTAACTCTAGACTCTAGAACCCATTCTAGCATATCTCACGTGCAATTTTTAAAAATAAGTTAAT AATTCATCTCATATCAACAAAAGCCTTTGAAACATGGGTTTTCACTAGATATCACCTAGTGCTAAGATAA AAACCAAAACAATATCAGAATTACATTTATGCTCTAAATTTGTAGTTGTCCATTGTTGTGCTTAGTAAAT GTGTGTCATTAAATTAATGCTGTATTCTCCTAGCTATTATGGAAACTTGTTTAAATAAAGATATGGATAT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK090859 unedited GGCGTTCANATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGGTCGCTT CTACAATCATTTCTGATGATAGCTTGGGAATAGAGATAGGGGCAGTGACTTGCCTGATGT CGCACAGCCCTCCGGCTGTCCTGCTTTCCCATATGGAGCAGTGGTGGTGTGGGCACCTGT GATGCAGGAGACTTTAAAAATGTCGTGAGGTCACGTGCTGCCCCTCCTGGTACGTGTGGA ATGCCCCTGGCCAGCAAGGGGTGCTTTTTTATCAGAGTTGGCAGCTGGCATGTGGGAACC GAGCAAGTGCTGCGTACCAAGTTACTTGTTTTAAGGAGACCAAGTGCTCAGCGCCAGGTG GTTTTCTTTTTTGTCATAGTTACTTGCTATAACTCAGCTTGACTTCTGTCATGAATCAGT GCTCTCTGGGAGGATGCAATACTCTGTTTGGGCATTAATTGGTAGCAGGTTGTCTCAACC AAAAAGACAGGAAACAGCAAAAGCCTCTCTGAAATTAAGAGGAAAGTTACTCTCCCCACA CCCATCAGAGTCTTTATTGGAGCCACCAGGTGAGCTGTGCAGCCTGGACAGGCCTGCAGC TATAGGCCACCTTCCCAGTTTAGGTCCTCAGCACAGGGGAGCCCAAGTCACTGGGTGCCT TCTGAGGGCTGTCACTGGGCAGGCCATATACAAATCAGTGTGTGCGTGGGCACTGCAGTG TGTGCATGCCGTANGTGTTGATGGGTGCTANGANGGGNTGTCGTGTGCATGCGCGTTGAA AAGGATCTGTATTGCCGTGACCTCTGTTCATGGATGAGTGCATTGTAATTTGTTCTCAGG CTGTGCTGTGGAGGCCGNCTTAACCCTTGCTCCTTNCACTTNCTAGACTGCCTTAAGTTC TACANAACTTTTACTCTGTCAAGGACTATCTGCCTGGNAAGGGAATATCTTGCCT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK090859 unedited NGGGTATTCTATGNACGCGGCCGCATTCTANNGATCGGTTTTTTTTTTTTTTTTTTTTTA TATCCATATCTTTATTTAAACAAGTTTCCATAATAGCTAGGAGAATACAGCATTAATGAC ACACATTTACTAAGCACAACAATGGACAACTACAAATTTAGAGCATAAATGTAATTCTGA TATTGTTTTGGTTTTTATCTTAGCACTAGGTGATATCTAGTGAAAACCCATGTTTCAAAG GCTTTTGTTGATATGAGATGAATTATTAACTTATTTTTAAAAATTGCACGTGAGATATGC TAGAATGGGTTCTAGAGTCTAGAGTTAGTTGATAATGCCTTTCATGTCTCAGTATTTCTG CTTCCTTTTTAATGGGTAAATGTTTCACCTAAGAACTTTCAAAAAATTGTATCAAAATTT TCTATGCAGATCAAAGTAGAATTCCTTTTCCAGACCCAACAAGGAAAAATATTTGAAAAC TGCAATGTTCAATTCCATTTTAGTTAAAAGGATGTCCATTTCATAGTAAATTAATACAGT TAATAATTAAAATACAATATGTTAAATGGCATGAACTCATTTTTGCGCAACACTGTTAGC GCAATTTAAATCTCCCAAAGACACAAAAAAGTCAATTTTACTGCAGGATCCCACAAAAAT ACATAACCAGGAATCATAAATTACAACATATATATCCACATTTAAAGAAAAAATTCACCA TTTGTTGGGAAAAAATAATGGCTCAAATGATTTACGGAGCCTGAAATGTGGGCTAAAATT TTATAACATGATAACAATAAGATTTGAAACTGCTGACTCTGAAAAATGCTAANAATTACT AACCTGCCAAGCACTGAATTATATATACCAGTTTCAACTAANAAACACATTTTTTCTTTC AAATATTCAG
Restriction Sites	NotI-NotI
ACCN	AK090859
Insert Size	2250 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK090859.1
RefSeq Size	2030 bp
RefSeq ORF	2030 bp
Locus ID	4644
Gene Summary	This gene is one of three myosin V heavy-chain genes, belonging to the myosin gene superfamily. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage, spindle-pole alignment and mRNA translocation. The protein encoded by this gene is abundant in melanocytes and nerve cells. Mutations in this gene cause Griscelli syndrome type-1 (GS1), Griscelli syndrome type-3 (GS3) and neuroectodermal melanolysosomal disease, or Elejalde disease. Multiple alternatively spliced transcript variants encoding different isoforms have been reported, but the full-length nature of some variants has not been determined. [provided by RefSeq, Dec 2008]

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