ORMDL1 (AF395704) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC102263 sequence for AF395704 edited (data generated by NextGen Sequencing) ATGAACGTTGGAGTTGCCCACAGTGAAGTGAATCCAAATACCCGTGTCATGAACAGCCGG GGTATGTGGCTGACATATGCATTGGGAGTTGGCTTGCTTCATATTGTCTTACTCAGCATT CCCTTCTTCAGTGTTCCTGTTGCTTGGACTTTAACAAATATTATACATAATCTGGGGATG TACGTATTTTTGCATGCAGTGAAAGGAACACCTTTCGAAACTCCTGACCAGGGTAAAGCA AGGCTCCTAACTCATTGGGAACAACTGGACTATGGAGTACAGTTTACATCTTCACGGAAG TTTTTCACAATTTCTCCAATAATTCTATATTTTCTGGCAAGTTTCTATACGAAGTATGAT CCAACTCACTTCATCCTAAACACAGCTTCTCTCCTGAGTGTACTAATTCCCAAAATGCCA CAACTACATGGTGTTCGGATCTTTGGAATTAATAAGTATTGA Clone variation with respect to AF395704.1 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF395704 unedited GACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTAGCAGCCGGGCTGGTCCTGCTGC GAGCCGGCGGCCCGGAGTGGGGCGGCGGAGCAAACATGAACGTTGGAGTTGCCCACAGTG AAGTGAATCCAAATACCCGTGTCATGAACAGCCGGGGTATGTGGCTGACATATGCATTGG GAGTTGGCTTGCTTCATATTGTCTTACTCAGCATTCCCTTCTTCAGTGTTCCTGTTGCTT GGACTTTAACAAATATTATACATAATCTGGGGATGTACGTATTTTTGCATGCAGTGAAAG GAACACCTTTCGAAACTCCTGACCAGGGTAAAGCAAGGCTCCTAACTCATTGGGAACAAC TGGACTATGGAGTACAGTTTACATCTTCACGGAAGTTTTTCACAATTTCTCCAATAATTC TATATTTTCTGGCAAGTTTCTATACGAAGTATGATCCAACTCACTTCATCCTAAACACAG CTTCTCTCCTGAGTGTACTAATTCCCAAAATGCCACAACTACATGGTGTTCGGATCTTTT GAATTATAAGTATTGAAATGTTTTGAAACTGAAAAAAATTTACAGCTACTGAATTTCTTA TAGGAAGGAGTGTTAGTAAACTGCACTGTTTCTCTGATAATGTTGAATGAGAAGTATTTA CATTGGAGGGCCCATGGCTGGTCCTTCAAGTGCTTGTTTGAAGGCAGATTTTCATTAATG ATGCCTCTGTTTATACCACCTGTACATTTCTGAAAAGGGGCTTTATAAGCAGGCTGGGCA GCCCAGCTTATTAGTAAGGGCATCCAGTGAGGGTTGTATAATAAATCAAGGAATAGAGAT TGTAGAACTAGACAGCTTACTATATGATGGGCATTGGTAGAAAGACATTCCGTCTTCGCT GGGTATAAGCAAATTCTGTAACGNATCTTTTTCAAT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AF395704 unedited CTATGTACCGCGGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTACGGTAATAA AAATAATCTTTAATAAACTTGTATAGAGAGGATTCCGGTTTACATGTAAGTCTGCTTTAA ATAACCACAGCTGAATGACTGGAAATGTTCTTTTCTTAACACAATGCCCATTCATTATAA GTTAAGCTGGTCCTAGTTTCTTACAAATCTCTTATTTCCTTGAATTTATCTACTACACCC TCACTGTGATGCCCTTTAACTTATAAGCTGGGCCTGCCCAGCCTGCTTATAAAGCCCCTC TTCAGAAATGTACCAGGTGTATTAAACAGAGGCATCATTTAATGGAAATCTGCACTTCAA AACAGCACTTGAAGGACCAGCCATTGGCCCTCCAATGTAAATACTTCTCATTTCACATTA TCAGAGAAACAGTGCAGTTTACTAACCACTCCTTCCTTATAAGAAATTCAGTAGCTGTAA AATTTTTTTTCAGTTTCAAAACATTTCAATACTTATTAATTCCAAAGATCCGAACACCAT GTAGTTGTGGCATTTTGGGAATTAGTACACTCAGGAGAGAAGCTGTGTTTAGGATGAAGT GAGTTGGATCATACTTCGTATAGAAACTTGCCAGAAAATATAGAATTATTGGAGAAATTG TGAAAAACTTCCGTGAAGATGTAAACTGTACTCCATAGTCCAGTTGTTCCCAATGAGTTA GGAGCCTTGCTTTACCCTGGTCAGGAGTTTCGAAAGGGTGTTCTTTCACTGCATGGCAAA ATACGTACATTCCCAGNATATGTATAATATTTGGTAAAGTCCAAGCACAGGAACACTNGA GAAGGGAATGCTGNAGTAGACATATGAAGCAGCCAACTCCCATGCTATGTCAGCACATAC CCCGCTGTTATGAACCGGTATTGGATTACTTACTGTGGGCACTCCACGTNATGTTGCTCC CCGCCCACTCCGGCCCCGCTCCANAAGACA
Restriction Sites	NotI-NotI
ACCN	AF395704
Insert Size	1080 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AF395704.1, AAM43503.1
RefSeq Size	1092 bp
RefSeq ORF	1092 bp
Locus ID	94101
Domains	ORMDL
Protein Families	Transmembrane
Gene Summary	Negative regulator of sphingolipid synthesis.[UniProtKB/Swiss-Prot Function]

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