STING (TMEM173) (AK095896) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ERIS; hMITA; hSTING; MITA; MPYS; NET23; SAVI; STING
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK095896, the custom clone sequence may differ by one or more nucleotides AAACCAGAAGAAAAATATGAGACGGGGAATCATCGTGTGATGTGTGTGCTGCCTTTGGCTGAGTGTGTGG AGTCCTGCTCAGGTGTTAGGTACAGTGTGTTTGATCGTGGTGGCTTGAGGGGAACCCGCTGTTCAGAGCT GTGACTGCGGCTGCACTCAGAGAAGCTGCCCTTGGCTGCTCGTAGCGCCGGGCCTTCTCTCCTCGTCATC ATCCAGAGCAGCCAGTGTCCGGGAGGCAGAAGATGCCCCACTCCAGCCTGCATCCATCCATCCCGTGTCC CAGGGGTCACGGGGCCCAGAAGGCAGCCTTGGTTCTGCTGAGTGCCTGCCTGGTGACCCTTTGGGGGCTA GGAGAGCCACCAGAGCACACTCTCCGGTACCTGGTCCTCCACCTAGCCTCCCTGCAGCTGGGACTGCTGT TAAACGGGGTCTGCAGCCTGGCTGAGGAGCTGCGCCACATCCACTCCAGGTGACTCACTGCAGTACCCAG GGACGGGGTATCCAACGTGTGTCACTCCCTTGATGCCTAGCCCTGCCCCTCCTTGAACCTCTCTGGCTGA GCTGGGCTGGGGGCTGGGGTCTGGGGTCTGGCTGTCACTCACAGGTACCGGGGCAGCTACTGGAGGACTG TGCGGGCCTGCCTGGGCTGCCCCCTCCGCCGTGGGGCCCTGTTGCTGCTGTCCATCTATTTCTACTACTC CCTCCCAAATGCGGTCGGCCCGCCCTTCACTTGGATGCTTGCCCTCCTGGGCCTCTCGCAGGCACTGAAC ATCCTCCTGGGCCTCAAGGTATGACACAGGGGGAGGTAGAAGCTCTGGCCAAGTGGTGGCTGTGGCTGGT GTGACCTGCCCTGAGCTGAGTACTGGGAGTGGGACTGGTTTAAAGGCTGGAGTCCATGGAGTAGAACCTA TAATGTCCTGGAACAGTGGGTTTGGCAATGGCAAAAGAGGGATCAAGTCAGGAGCAGGTTGGGAAGCCTT GGAGGAGGAGGAGGAGTTCTCTGGGTGTCCTTGATGGAGGCCCCCCAGCCACATCCTGCTGTCCACAGGG CCTGGCCCCAGCTGAGATCTCTGCAGTGTGTGAAAAAGGGAATTTCAACGTGGCCCATGGGCTGGCATGG TCATATTACATCGGATATCTGCGGCTGATCCTGCCAGCCTGGAGTACAGTGGTGACAAGATCTCAGCTCA CTGCAACCTCTGCCTCCCAGGTTCAAGTGATTCTCCTGCCTGAGCCTCTTGAATAGCTAGGATTACAGGC ACGTGCCACCATGCCTGGCTAATTTTTGTATTTTTAGTAGAGACAATGTTTCACCATGTTGCCCAGGCTA GTCTCAAACTCCTGACCTCAAATGATCCACCTGCCTCAGCCTCCCAAAGTGCTGGATTACAGGCGTGAGC CACTGTGCCTGCCCTAAGCCTGTGTGTTTTATTCTTCTGACTTGCAGGCTAAAGCGGCAGCTCTTCCATA TCTCATTGCTATCTCCTAGGGCTTCCGCTAGGAGACTGATCTGGGGCTAGAGGCCTCCCTCTGTGCACAC GAGAATGCTGGAAATGTCACCTCTCAGGGCTCTGCCTGCCTCTCAGCCCTGAAAGCCATGGTGGAAAGGG GTGGCGCTGACATAGACATCTGAGGAAAGAAGTGAGGGAGGGTAAAGGGTGGTGCAGTAAGAGGAGGGGT GGGGAGGGCTTTTGGAGGCGCTGCCCCTCCTGGCCTCCTGTACAATGAGAGTGCACTGGACTCTCCATTC TCTGGCACCCACACACTGCGGGGGCCAATGACCTGGGTCTCACTCCTGAATCAGGTGGGAGATAGGGTTA GCAGGAATAACTTCTTGGGCTTCCCTGCCTCAGAGCTCCAGGCCCGGATTCGAACTTACAATCAGCATTA CAACAACCTGCTACGGGGTGCAGTGAGCCAGCGGCTGTATATTCTCCTCCCATTGGACTGTGGGGTGCCT GATAACCTGAGTATGGCTGACCCCAACATTCGCTTCCTGGATAAACTGCCCCAGCAGACCGGTGACCGTG CTGGCATCAAGGATCGGGTTTACAGCAACAGCATCTATGAGCTTCTGGAGAACGGGCAGCGGGCGGGCAC CTGTGTCCTGGAGTACGCCACCCCCTTGCAGACTTTGTTTGCCATGTCACAATACAGTCAAGCTGGCTTT AGCCGGGAGGATAGGCTTGAGCAGGCCAAACTCTTCTGCCGGACACTTGAGGACATCCTGGCAGATGCCC CTGAGTCTCAGAACAACTGCCGCCTCATTGCCTACCAGGAACCTGCAGATGACAGCAGCTTCTCGCTGTC CCAGGAGGTTCTCCGGCACCTGCGGCAGGAGGAAAAGGAAGAGGTTACTGTGGGCAGCTTGAAGACCTCA GCGGTGCCCAGTACCTCCACGATGTCCCAAGAGCCTGAGCTCCTCATCAGTGGAATGGAAAAGCCCCTCC CTCTCCGCACGGATTTCTCTTGAGACCCAGGGTCACCAGGCCAGAGCCTCCAGTGGTCTCCAAGCCTCTG GACTGGGGGCTCTCTTCAGTGGCTGAATGTCCAGCAGAGCTATTTCCTTCCACAGGGGGCCTTGCAGGGA AGGGTCCAGGACTTGACATCTTAAGATGCGTCTTGTCCCCTTGGGCCAGTCATTTCCCCTCTCTGAGCCT CGGTGTCTTCAACCTGTGAAATGGGATCATAATCACTGCCTTACCTCCCTCACGGTTGTTGTGAGGACTG AGTGTGTGGAAGTTTTTCATAAACTTTGGATGCTAGTGTACTTAGGGGGTGTGCCAGGTGTCTTTCATGG GGCCTTCCAGACCCACTCCCCACCCTTCTCCCCTTCCTTTGCCCGGGGACGCCGAACTCTCTCAATGGTA TCAACAGGCTCCTTCGCCCTCTGGCTCCTGGTCATGTTCCATTATTGGGGAGCCCCAGCAGAAGAATGGA GAGGAGGAGGAGGCTGAGTTTGGGGTATTGAATCCCCCGGCTCCCACCCTGCAGCATCAAGGTTGCTATG GACTCTCCTGCCGGGCAACTCTTGCGTAATCATGACTATCTCTAGGATTCTGGCACCACTTCCTTCCCTG GCCCCTTAAGCCTAGCTGTGTATCGGCACCCCCACCCCACTAGAGTACTCCCTCTCACTTGCGGTTTCCT TATACTCCACCCCTTTCTCAACGGTCCTTTTTTAAAGCACATCTCAGATT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK095896 unedited AAATTTTGTATACGACGTCACTATAGGGCGGCCGCGAATTCGCACGAGGACATAGACATC TGAGGAAAGAATGAGGGAGGGTAAAGGGTGGTGCAGTAAGAGGAGGGGTGGGGAGGGCTT TTGGAGGCGCTGCCCCTCCTGGCCTCCTGTACAATGAGAGTGCACTGGACTCTCCATTCT CTGGCACCCACACACTGCGGGGGCCAATGACCTGGGTCTCACTCCTGAATCAGGTGGGAG ATAGGGTTAGCAGGAATAACTTCTTGGGCTTCCCTGCCTCAGAGCTCCAGGCCCGGATTC GAACTTACAATCAGCATTACAACAACCTGCTACGGGGTGCAGTGAGCCAGCGGCTGTATA TTCTCCTCCCATTGGACTGTGGGGTGCCTGATAACCTGAGTATGGCTGACCCCAACATTC GCTTCCTGGATAAACTGCCCCAGCAGACCGGTGACCATGCTGGCATCAAGGATCGGGTTT ACAGCAACAGCATCTATGAGCTTCTGGAGAACGGGCAGCGGGCGGGCACCTGTGTCCTGG AGTACGCCACCCCCTTGCAGACTTTGTTTGCCATGTCACAATACAGTCAAGCTGGCTTTA GCCGGGAGGATAGGCTTGAGCAGGCCAAACTCTTCTGCCGGACACTTGAGGACATCCTGG CAGATGCCCCTGAGTCTCAGAACAACTGCCGCCTCATTGCCTACCAGGAACCTGCAGATG ACAGCAGCTTCTCGCTGTCCCAGGAAGTTCTCCGGCACCTGCNGCANGANGAAAANGGAA GAGTTACTGTGGGCAGCTTGAAGACCCTCAGCGTGCCCAGTACCTNCACGATGTCCCAAG AGCCCTGAGCTCTCATCAGTGGAATGGNAAAAGCCCTNCCTCTCTNACNGATTCTCTTGA GACCAGGGTACCAGCCAGAGCCTNCAGTGTCTCCAGCTCTGACTGGGGCTCTCTCATGGC TGAATGTCACANACTATTCCTTCAGGGGNCTGCAGGGA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK095896 unedited GAATACTTGTTACGCGGCCGCAATCCACGATCGATTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTAATCTGGAAAGTGCTTTAAAAAAGACCGTTTAAAAAGGGT GGAGTATAAGGAAACCCCAAGTGAAAGGGAGTACTCTATTGGGGTGGGGGTCCCCATACA CAACTAGGCTTAAGGGGCCAGGGAAAGAAATGGTGCCAAAATCCTAGAAATAGTCATGAT TACCCAAGAATTGCCCGGCCGGAAATTCCATACCACCCTTTATTCTCCCAGGTGGGACCC CGGGGAATCAATTCCCCAAACTCATCCTCCTCCTCCTCTCCATTTTTCTGCTGGGGCTCC CCAATAATGGAACATTGACCAGGACCCAAGGCCCTAAAGACCCTGTTTACCCCATTGAAA GAGTTCGGCGTCCCGGGGACAAGGAAGGGGAAAAGGGGGGCGAGTGCGCTCCTAGACACT ATGTAACGCACCACGGACATACCCCAGCACAAGTCCTACACAAACCTACAAGTCTAAATG TGACCTACTCCACGCGGAAGAGGGGAACACTGGGAGGAGTGAGAGGAGTGAATCACCAGA ATCAGCACACCACAACAGCACGACGAACAAACAGGACAGAGTGACGCGAGCGGTGAACAG CAAGGGGCGCGAAACCGCAAACAAAAAAACCCCACACATACAGGAGAAAACGCGAACGAA CAAGAGTACAAAACAAAGACACAAGAACAGGAAGAAAAATAAAGTATACCGAGCACGTAG CATCGACATTACGGAGTTACGTAAACTTGCCCACATCCTGGTAAGATATGTTTAATATTC GGTGATTCCGCAAATTTTCACCCGCCGGGCGGCACAGGCTCCCACCACCCGCCCCGACGA ACGGAAAGCAGCGCAGAACGGGCCAA
Restriction Sites	NotI-NotI
ACCN	AK095896
Insert Size	1750 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK095896.1
RefSeq Size	3200 bp
RefSeq ORF	3200 bp
Locus ID	340061
Protein Pathways	Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway
Gene Summary	This gene encodes a five transmembrane protein that functions as a major regulator of the innate immune response to viral and bacterial infections. The encoded protein is a pattern recognition receptor that detects cytosolic nucleic acids and transmits signals that activate type I interferon responses. The encoded protein has also been shown to play a role in apoptotic signaling by associating with type II major histocompatibility complex. Mutations in this gene are the cause of infantile-onset STING-associated vasculopathy. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]

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