POLR3A (AK024744) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ADDH; HLD7; hRPC155; RPC1; RPC155
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK024744, the custom clone sequence may differ by one or more nucleotides AATCCTTTACACCATGGTGAACCACGGCATGAGCATCGACAGGAGGCACGTGATGCTGCTCTCCGACCTC ATGACCTACAAGGGTGAAGTCCTGGGCATCACTAGGTTTGGCCTGGCCAAGATGAAGGAGAGTGTGCTGA TGCTGGCCTCCTTTGAGAAGACGGCTGACCATCTCTTTGACGCTGCCTACTTCGGGCAGAAGGACTCTGT GTGTGGGGTGTCTGAGTGCATCATCATGGGAATCCCAATGAACATTGGAACCGGGCTCTTCAAGCTGCTT CACAAGGCTGACAGGGACCCGAACCCTCCCAAGAGGCCCCTGATCTTCGACACAAATGAATTCCACATCC CCCTTGTCACATAGTCCAAAGAAAGAGGGGACCATGCCTGACCTTGACTCCTTGTCCTGTCTCCAGCTGA TGTATAAAGAGTTTTGTGCTCCCTGGGACGGGGGTCCTGAGGTCCCCACCTATGCCAGCAATCAGAGAAG CCCTCTTGGCATCCCCAGGAGCAGCTTCTCCTCTGATAGGGTGCAGCTCACACCAGTGACCCTGACTGTG CCACGCTGCTCGGGAGAGCTGAGGGTTTTATTGTTTGCTTGCTTGAAACCTAATCTATAGACGGCCCCAC AGCTCGTGCACACACTGCTTCCCTGGACTTAAAGCCCAGCCAGGTGTCATGGTCCATCCCAGCTCACTGC ATACATCCATCGGCTCCCCATGGTGTCTTCACACCTGACGATGAGCCAGGCCTGAGCCCCACACAGGCCA GGGCACATTCTTGGATTTTCCATTCCTTGGTCATGCTGGAATCTCTCAATGTGACATACTTATGTAAATA TTGTTACTATTATTTATTTGTTCCATTTGAGGGATTTGGAATTTTTGTTATTTTAGTTTTATTTTTGAAA CCAAGCATCTATAGAAACCAAGAAAGTCAGCATGTAAGCGTCACTGGAAAAACTGGTTTAAGCAAATAGA GCCGTCTGGGATTTGTAACTGAGGTGCAACTGTCATGAGGCCCAGGCAGCTCTGTAACATCTTCTATAGA TGCCCCTGGCTACCCTGTTGTTTTCATCTACCTCAGACCCCTATCATGGGGCTCTACCCTGTGACAAGAG CCAAACCCATTCTCCATGGCCTATGGAAGCCTCACTGGAGTTTGGGGCCTGCTGCAATGGGGATGAGATG GTTTTTTGTAGAATTATACTTACGTTCCTTGGATGATCTCTAGTTGATTTTTTAAGTTCTGAGTTGATGC TGTTAAGGTACCCGGGGTAGCCATTGGTTCTTGGATCTGTGTTAGAATGAGTGCTTTCCCTTCCTACTGA TGTGATTGTGGATTAGGAATTCGTGACCGAGTGATTTTTGGCCAGTGGTTGGGTTTAAAATTCTATTAAA ATTTGTAGTTTGGGCTGGGTGCTGTGGCTCATACCTATAATCCCAGCACTTTGGGAGGCCGAGGGGGGCA GATCACTTGAGCCCAGGAGTTCGAGAGCAGCCTGGGCAACATGACGAAACCCCGTCTCTACAGAGAATAC AAAAATTAGCCAGGCATGGTGGCCCATGCCTGTAGTACCTGCTATTGGTAAGGCTGAGGTGGAAGGTTCA CTTGAGCCTGGGAGGTGGAGGTTGTAGTGAGCCAAGATCGTGCCACTACACTCCAGCCTGGGCAACAAAG CAAGACCTTGTCTCAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK024744 unedited TTTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGGCAAGAGCTCCAT GTACTACGTGCTGCAGTTCCTGAAAGAGGATCTCCCCAGGTGGTGGTGCAGGGCATTCCA GAGGTGTCCAGAGCTGTCATCCACATTGACGAGCAGAGTGGAAAGGAGAAGTACAAGCTT CTGGTGGAAGGTGATAACCTGCGGGCAGTCATGGCCACACACGGTGTGAAGGGCACCCGA ACCACCTCCAATAACACCTATGAGGTGGAGAAAACTCTGGGCATCGAGGCCGCCCGGACA ACGATCATCAATGAAATCCAGTACACCATGGTGAACCACGGCATGAGCATCGACAGGAGG CACGTGATGCTGCTCTCCGACCTCATGACCTACAAGGGTGAAGTCCTGGGCATCACTAGG TTTGGCCTGGCCAAGATGAAGGAGAGTGTGCTGATGCTGGCCTCCTTTGAGAAGACGGCT GACCATCTCTTTGACGCTGCCTACTTCGGGCAGAAGGACTCTGTGTGTGGGGTGTCTGAG TGCATCATCATGGGAATCCCAATGAACATTGGAACCGGGCTCTTCAAGCTGCTTCACAAG GCTGACAGGGACCCGAACCCCTCCAAGAGCCCCTGATCTTCGACACANATGAATCCACAT CCCCCTTGTCACATAGTCCAAAGAAAGAGGGACCATGCCTGACCTTGACTNCTTGTCCTG TCTCCAGCTGATGTATAAAAGATTTTTGTGCTCCCCTGGGACGGGGGGTCCTGAAGGTCC CCACCTATGCCCACCCATCAAAGAAGCCCTTCTTGGGAATCCCCAGAAGCAGTCTCTTCC TCTGGATAGTTGCAAGCTCACACCATTGACCTTGACTGGCCCCCCTGCTCGGGAAACCTG AGGGTTTTATATGGTGCTTGCTTGAACCCTAT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK024744 unedited GGTCATATTTATGNACCGCGGCACGCAATTTAGNGATCGAGTTTTTTTTTTTTTTTTTTG AGACAAGGTCTTGCTTTGTTGCCCAGGCTGGGGGGGAGTGGCACGATCTTGGCTCACTAC AACCTACACCTCCCAGGCTCAAGTGAACCTTCCACCTCAGCCTTACCAATAGCAGGTACT ACAGGCATGGGCCACCATGCCTGGCTAATTTTTGTATTCTCTGTATATACGGGGTTTCGT CATGTTGCCCAGGGGGGTCTCGAACTCCTGGGCTCAAGTGATCTGCCCCCCTCGGCCTCC CAAAGTGCTGGGATTATAGGTATGAGCCACAGCACCCAGCCCAAACTACAAATTTTAATA GAATTTTAAACCCAACCACTGGCCAAAAATCACTCGGTCACGAATTCCTAATCCACAATC ACATCAGTAGGAAGGGAAAGCACTCATTCTAACACAGATCCAAGAACCAATGGCTACCCC GGGTACCTTAACAGCATCAACTCAGAACTTAAAAAATCAACTAGAGATCATCCAAGGAAC GTAAGTATAATTCTACAAAAAACCATCTCATCCCCATTGCAGCAGGCCCCAAACTCCAGT GAGGCTTCCATAGGCCATGGAGAATGGGTTTGGCTCTTGTCACAGGGTAGAGCCCCATGA TAGGGGTCTGAGGTAGATGAAAACAACAGGGTAGCCAGGGGCATCTATAGAAGATGTTAC AGAGCTGCCTGGGCCTCATGACAGTTGCACCTCAGTTACAAATCCCAGACGGCTCTATTT GCTTAAACCAGTTTTTCCAGTGACGCTAACATGCTGACTTNTCTGGGTTCTATAGATGCT TGGTTTCAAAAATAAACTAAAATAACCAAATTTCCAATCCCTCAAATGGGACAAATTAAT TATAGTTACCATT
Restriction Sites	NotI-NotI
ACCN	AK024744
Insert Size	2100 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK024744.1
RefSeq Size	1715 bp
RefSeq ORF	1715 bp
Locus ID	11128
Protein Families	Transcription Factors
Protein Pathways	Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
Gene Summary	The protein encoded by this gene is the catalytic component of RNA polymerase III, which synthesizes small RNAs. The encoded protein also acts as a sensor to detect foreign DNA and trigger an innate immune response. [provided by RefSeq, Aug 2011]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.