RIF1 (AK001461) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK001461, the custom clone sequence may differ by one or more nucleotides GTTATTAATCAAAATGCAAAGCAGTTGGAAAATAAGGAGCATCTCTGGAAAATGTGGAGTGTTATAGTCA CCCCATTAACTGAATTGATTAATCAGACCAATGAAGTAAATCAAGGTGATGCCTTAGAACATAATTTTAG TGCCATCTATGGTGCATTGACTTTACCAGTAAACCACATTTTTTCAGAACAGAGATTTCCAGTGCCCACC ATGAAGACTTTGCTTAGAACTTGGTCAGAATTATATAGAGCATTTGCTCGTTGTGCTGCTTTGGTGGCAA CAGCAGAAGAGAACTTGTGCTGTGAGGAACTTTCTTCCAAGATAATGTCCAGTTTGGAAGATGAAGGCTT TTCTAATTTGTTGTTCGTGGATAGAATTATTTATATTATTACTGTAATGGTTGATTGCATTGACTTCTCA CCATATAATATTAAATATCAGCCCAAAGTTAAATCACCACAGAGACCTTCAGATTGGTCCAAAAAGAAGA ATGAGCCCCTAGGGAAATTGACTTCTTTATTTAAACTTATTGTGAAAGTGATCTATTCTTTCCACACACT GAGCTTCAAGGAAGCACATTCTGATACCCTCTTCACTATTGGCAACTCAATCACCGGCATTATTTCCAGT GTACTTGGGCATATTTCTTTGCCTTCTATGATCCGAAAAATATTTGCAACTTTAACAAGACCTCTGGCAT TATTTTATGAAAACTCAAAGCTTGATGAAGTTCCTAAAGTATATAGTTGTCTGAACAACAAGTTAGAAAA GCTACTGGGAGAAATTATTGCTTGTCTGCAATTCAGCTACACCGGAACTTATGATAGTGAACTTCTTGAA CAACTCTCCCCACTATTATGCATAATATTTCTGCACAAGAATAAACAGATTCGAAAACAGAGTGCTCAGT TCTGGAATGCCACTTTTGCCAAAGTGATGATGTTGGTTTATCCTGAAGAGTTAAAACCAGTACTAACACA AGCCAAACAAAAATTTCTGCTCCTGTTGCCTGGTTTGGAAACTGTTGAAATGATGGAGGAATCCAGTGGA CCATATTCTGATGGAACAGAAAATTCACAACTAAATGTGAAGATAAGTGGCATGGAGAGAAAATCAAATG GAAAAAGAGATTCATTTTTGGCACAAACAAAGAATAAAAAAGAAAATATGAAACCAGCAGCCAAACTGAA ACTTGAATCTTCGTCTTTAAAAGTAAAGGGTGAAATTCTTTTGGAAGAGGAAAAGTCTACTGACTTTGTG TTTATACCTCCAGAAGGAAAAGATGCAAAGGAAAGAATATTAACTGATCATCAAAAAGAAGTTCTCAAAA CAAAGCGGTGTGATATTCCTGCCATGTATAATAATCTGGATGTTTCCCAAGATACCTTATTTACTCAGTA TAGTCAGGAAGAGCCTATGGAAATTCCTACTTTAACCAGAAAACCAAAGGAGGATTCTAAGATGATGATT ACGGAGGAGCAAATGGACAGTGACATTGTCATTCCTCAAGATGTCACGGAAGACTGTGGTATGGCTGAAC ATCTTGAAAAGTCCTCCCTTTCGAATAATGAGTGTGGTTCTCTTGACAAAACCAGTCCAGAAATGTCAAA CAGTAATAATGATGAAAGAAAAAAAGCTTTAATTTCATCAAGGAAAACATCAACTGAATGTGCATCTAGT ACAGAAAATTCTTTCGTTGTCAGCAGTAGTTCAGTTTCTAATACCACTGTTGCTGGAACTCCCCCATACC CTACAAGTCGGAGGCAAACCTTTATTACTTTGGAGAAGTTTGATGGTTCAGAAAATAGACCTTTTAGTCC ATCCCCCTTGAATAATATTTCATCAACTGTTACAGTGAAAAATAACCAGGAAACCGTGATTAAAACAGAT TTTCTACCAAAAGCAAAGCAAAGAGAAGGGACTTTTTCAAAATCTGATTCTGAAAAAATAGTGAATGGAA CTAAGAGATCAAGCCGGAGAGCTGGTAAAGCTGAACAAACAGGGAATAAAAGGTCTAAGCCCTTAATGAG ATCTGAGCCGGAGAAAAATACTGAGGAATCTGTTGAAGGCATTGTAGTCTTAGAAAATAACCCACCTGGT TTGCTTAATCAAACAGAATGTGTGTCAGATAATCAGGTTCATCTTTCTGAATCTACAATGGAGCATGACA ATACAAAGCTTAAAGCAGCAACAGTGGAAAATGCTGTATTATTGGAAACTAATACTGTAGAGGAGAAAAA TGTAGAAATTAATTTGGAATCCAAAGAGAATACACCCCCAGTAGTAATATCAGCAGATCAAATGGTAAAT GAGGATAGTCAGGTTCAGATAACTCCAAATCAGAAAACCCTTAGACGGTCTTCAAGGCGACGTTCAGAAG TAGTAGAGTCTACCACTGAAAGCCAAGATAAGGAAAATAGTCATCAAAAAAAGGAACGACGTAAGGAAGA AGAAAAACCTCTTCAGAAGAGTCCATTGCATATAAAAGATGATGTGTTACCTAAACAAAAACTGATTGCT GAACAAACTCTACAGGAGAATTTAATTGAGAAAGGAAGTAATTTACATGAGAAGACTCTTGGGGAAACTA GTGCTAATGCAGAAACTG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK001461 unedited GGGGTTCACATTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGATGAAA GTTCTTTCTCAGTTTGGAATCACTTGTAGGCTGTGTTCTTTCTGGTCCAACTTCACCACT AGCTTTCAGTGACTCAGTTTTAAATGTTATTAATCAAAATGCAAAGCAGTTGGAAAATAA GGAGCATCTCTGGAAAATGTGGAGTGTTATAGTCACCCCATTAACTGAATTGATTAATCA GACCAATGAAGTAAATCAAGGTGATGCCTTAGAACATAATTTTAGTGCCATCTATGGTGC ATTGACTTTACCAGTAAACCACATTTTTTCAGAACAGAGATTTCCAGTGGCCACCATGAA GACTTTGCTTAGAACTTGGTCAGAATTATATAGAGCATTTGCTCGTTGTGCTGCTTTGGT GGCAACAGCAGAAGAGAACTTGTGCTGTGAGGAACTTTCTTCCAAGATAATGTCCAGTTT GGAAGATGAAGGCTTTTCTAATTTGTTGTTCGTGGATAGAATTATTTATATTATTACTGT AATGGTTGATTGCATTGACTTCTCACCATATAATATTAAATATCAGCCCAAAGTTAAATC ACCACAGAGACCTTCAGATTGGTCCAAAAAGAAGAATGAGCCCCTAGGGAAATTGACTTC TTTATTTAAACTTATTGTGAAAGTGATCTATTCTTTCCACACACTGAGCTTCAAGGAAGC ACATTCTGATACCCTCTTCACTATTGGCAACTCAATCACCGGCATTATTTNCAGTGTACT TGGGCATATTTCTTTGCCTTCTATGATCCGAAAAATATTTGCAACTTTAACAAGACCTCT GGCATTATTTTATGAAAACTCNAAGCTGATGAAGTNNCTAAGTATATAGTTGTCTGAACA ACAAGTAGAAAGCTACTGGGAGAAATATTGCTT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK001461 unedited AGTATGGACCGCGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAACCCATGAGT GACTTTTCCTGAGGTTGTGATTTTTCCCCACAGCAATCACAACCTTTAGATCTCCTCACC CTCCTACTTCTCTTGTGTTGGCATTCAAGTGTTTGAAAAGTTTTTTCTGAAAGCGAAGAA ATATCTGATATCCCTATTTTTGAGGATTCTCCCAAACTACAATTATCAAAAGAGTCTCGC TTATGTAATCTCTTAATGGCATTTCTAGTCCTTAGATTTGATTCTGGCACAGGTAGACTT GTAAAGGAATATTCTGCATATTTGCTAGTTTCATTTTTTTCCTCACACACATTTGGTAAA TCATCAGGAACTTGCAACAAATCTGAAGTTACTGTAGAATCCTGACATATTACAGTATTA CTTTCATCTTGTTTTTCGCAAATCGGAGATTTTATGCTTACATCTTCTTCAGAAGTTGCT TTATAATCATGTGACTGATTTTCAGCTTTTATACATTCCTGTTTCACCACTTTCTCTTCT TGATCTTGAATGTCAACACTTTCATTGCTTTTGTTTTTCCATTTTCCAGATCTCTTCTTC CTAGAACCTTCTTCTTTTGCCTCCGAACTATCAGATTCTGAGTTTTCAATGCTGGAAAGC AAACCCTGAGATGCTCTTCTAGTTTGATACCGTGTTCGGCCTCTGACTAGTTTCTCAGAG GACTTATCTACAATGTCCTGNGTACCATCCCCCTCAGACTTAATGTTCTCAGGGTCTGCC TTTTTTTTATTTTATTCAGTTTCTGCATTAGCACTAGTTTCCCCAAGAGTCTTCTCATGT AAATTACTTCCTTTCTCATTAAATTCTCCTGTAGAGTTTGTTCAGCAATCAGTTTTTGTT TAGGTAACACATCATCTTTTATATGCAATGGACTCTTCTGAAGAGGTTTTTCTCTNCCTA CGTCGTCCTTTTTTGAGACT
Restriction Sites	NotI-NotI
ACCN	AK001461
Insert Size	3700 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK001461.1, BAA91705.1
RefSeq Size	2608 bp
RefSeq ORF	2608 bp
Locus ID	55183
Gene Summary	This gene encodes a protein that shares homology with the yeast teleomere binding protein, Rap1 interacting factor 1. This protein localizes to aberrant telomeres may be involved in DNA repair. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]

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