WDR68 (DCAF7) (AK025925) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AN11; HAN11; SWAN-1; WDR68
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK025925, the custom clone sequence may differ by one or more nucleotides AGACTGTCCATTGCATGGCTGTGGAGTGAGACTGCCCTTAGCCTGGGTCAGCCTTCCTGGGCCATAAATT GGGCATCCGTGATGCTAGGTAACTGTGGGAACAAAATGACAGCTTAGAGCAGCCATGGGTGATGTTTGGT GGTAAAAAACCTACAGGCGTTTGGGGTCCCATGATTGTTCCAGACCATGACTCTTCCTGGTTGTGGGTTT GTTACAGAGCAGGAGAAGCAGAGGTTATGACAGTTATGCAGACTTTCCCCCTCCTTTTTCTCTTTTCTCT TCCCCTTGCTTTTCCACTGTTTCTTCCTGCTGCCACCTGGGCCTTGAATTCCTGGGCTGTGAAGACATGT AGCAGCTGCAGGGTTTACCACACGTGGGAGGGCAGCCCAGTACTGTCCCTCTGCCTTCCCCACTTTGAGA ATATGGCAGCCCCTTTCATTCCTGGCTTGGGGTAGGGGAGACCATTGAAGTAGAAGCCTCAAAGCAGACT TTTCCCTTTACTGTGTGTACTCCAGGACGAAGAAGGAAGATCATGCTTGATACTTAGATTGGTTTTCCCA GGGAAGAGGGCGGAGCAGAGCAAAGTCACTGTGAACCCTGGGCCAGGCCCTGGCTGGGCCAGCTCCTGAG AGCGTCTCGTGTTGCAGACCCTTGCCCACTTCACCCACCTGCACCTTCTCCCCCTCTCACAGTGTCACTG CTGCTAATGGTCAAAGTCAAATGTGTGGCCACATGGGATGGGCCAGGTCCTCTCAGGCTACTTTCTGGAT GTCATTTTTAAAATATGGAAACATGCAGGTGCCTTCCCAAAGAGGCTTGGACTGGTATATCCAACGAGAA ACAAATAAGCTAAAGAAAGTTTAAACTCAAGAAGAAAGATGTTGACAGTCTATGTAACAGCTGGAAAGTT TATAGGCACCCACCTTTGGGACAACCCAGTGATTATGAACATGTGATATCTACTATTTAAAAGAAATGTT CTCACCTTGGGTTGATTGTGGTATACCATGTGTTATGAAAATTGTTGAGCTGAAGCTTTGAATCGATTTA GTTGAGTCTGACTCACTTGCTTTGGTTCCTGTGTATTTTACTACCCCTCTTGTCAGTGACCTTCCTTCCC CACCCCACCCAGAGTGAATTTGTAGCATGATTGTATAAACCTCTATGTAGAAAATGGAGATTTCTTGCTC TGAAATGTTAAGCTCTAACTGATCCATTTCTGTGTCCTTTAGCCTAGTATGTCTGAACTTCCATTCTTGT TATATATTTAAACTTTCCCTCTATATTATAGGTTTTGTGGCATCCACGGTCAGGTGTAGAGGAAGCTGCC CCTTGCAGAACTGTACTGTAATATTTTTCTTTTATAAATATTTTCACAGGACTGATTGTACACAGGGCTT GTAATAAAATTCTAACACTGTGCTGTGAAACAACTATGGGGAATCTCCATTGAAGGCTACTTCATGGGCA CCTGAAAGTGGAGTGTTATAGCTATGACTTTCTATTTCTTGTTTCCTAAGTAAATTAAACCTAATTTTCA CCCTTTCATTCTGTTTCAGCCTCCTGTATAAGAAGTACCGTATTTTCTGCCCATCATACTTTGTAATAAA ACTTGAACATGTAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK025925 unedited NGGGTCAAATTTGATACACTATTAGGCGGCGGATTCGCCGAGGAACTGGCTAAATTAGAA GAACAAAAAATCAGAATCCTACAAGGTTTTGATGACAATTAGGGCCAAAATTTTAGGAGG AGATGTAGGATGCAGGAGAAAATTAAAGTGTTTTCTTTATATCAGAGGAGGAAATAGTAG AGGTCAGTGAAGGTCTGGGGTAGGGAAACATTCAGACTGTCCATTGCATGGCTGTGGAGT GAGACTGCCCTTAGCCTGGGTCAGCCTTCCTGGGCCATAAATTGGGCATCCGTGATGCTA GGTAACTGTGGGAACAAAATGACAGCTTAGAGCAGCCATGGGTGATGTTTGGTGGTAAAA AACCTACAGGCGTTTGGGGTCCCATGATTGTTCCAGACCATGACTCTTCCTGGTTGTGGG TTTGTTACAGAGCAGGAGAAGCAGAGGTTATGACAGTTATGCAGACTTTCCCCCTCCTTT TTCTCTTTTCTCTTCCCCTTGCTTTTCCACTGTTTCTTCCTGCTGCCACCTGGGCCTTGA ATTCCTGGGCTGTGAAGACATGTAGCAGCTGCAGGGTTTACCACACGTGGGAGGGCAGCC CAGTACTGTCCCTCTGCCTTCCCCACTTTGAGAATATGGCAGCCCCTTTCATTCCTGGCT TGGGGTAGGGGAGACCATTGAAGTAGAAGCCTCAAAGCAGACTTTTCCCTTTACTGGTGT GTACTCCAGGACGAAGAAAGAAGATCATGCTTGATACTTAGAATTGGTTTTCCCAGNGGA AGAGGGCGGAGCAGAGCANAGTCACTGNTGAACCCTGGGCCANGCCCNTGGCTGGNGCCA GCNNTCTGAGAGCGTNCTCGTGGTTGCAGACCCCTTTGCCACTTNNNACCACCTGCACCT TCTTNCCCCTCTNACAGTGTCAA
Restriction Sites	NotI-NotI
ACCN	AK025925
Insert Size	2000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK025925.1
RefSeq Size	1638 bp
RefSeq ORF	1638 bp
Locus ID	10238
Gene Summary	This gene encodes a protein with multiple WD40 repeats which facilitate protein-protein interactions and thereby enable the assembly of multiprotein complexes. This protein has been shown to function as a scaffold protein for protein complexes involved in kinase signaling. This highly conserved gene is present in eukaryotic plants, fungi, and animals. The ortholog of this gene was first identified in plants as a key regulator of anthocyanin biosynthesis and flower pigmentation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

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