NPR2L (NPRL2) (AK098161) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	NPR2; NPR2L; TUSC4
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK098161, the custom clone sequence may differ by one or more nucleotides CGGGCTGTAGGGATCCGGTTGGGGGGTTCCAGGTCCCACAGAAGGTGAGTTGGGTGACCCATGAGTATCC CTGAACTGAGTGAAGAGTAAATGATGCATAGGAAGTTTAGGTAAGGCAGGAGAAAGTGTTCCAGGTAGAG GGAACAGCATATGCAGAAGCCAGGAAGGAGGTGAGATCATAGCGTATGTTTGGAAACAGAAAGAAGTAAT AGGCCTGAGTGGATGGGAGAGAGGGGAACGCTGGAGAAGAGGACTTGGGCTTGGGGGGCATGAGGAGGTC ACCATTTGTACTACACCCAGTACAGATGTCCACTGAGGGGCCCATTGCCACTCCCCTCCCCAGGTCCCTG AAGACTTCATCTCCCGAGAGCTGTTTGACACAGTCCAAGTGTACATCATCACCAAGCCAGAGCTGCAGAA CAAGCTTATCACTGTGTGAGACCCTAGCTCGGGGTGAGCGGTGGGTGGCAGGGGTTGTCCCAGGAGGAGG AAGGGACAGGCTTGGGGGCCTGACTGTGTTCCAATTATCCAGCACAGCTATGGAAAAGAAGCTGATCGGC TGTCCTGTGTGCATCGAACACAAGAAGTACAGCCGCAATGCTCTCCTCTTCAACCTGGGCTTCGTGTGTG ATGCCCAGGCCAAGACCTGCGCCCTCGAGCCCATTGTTAAAAAGCTGGCTGGCTATCTGACCACACTAGA GGTCTGCAATGAGATGGGCTTGGGTTTACCGACAGGCAGAGTTGGGGAAGGTGTTCCCATGTCTCCAAAG TCCCCCAAACCTGGGACCCTGCAAACCAGCTCAGGACTCAGGGCAGCCAACCAGCCAGGGGTCCTGGGAT GTGCCCACCATGCTTTCCATGCTCTGTCTAGCTAGAGAGCAGCTTCGTGTCCATGGAGGAGAGCAAGCAG AAGTTGGTGCCCATCATGACCATCTTGCTGGAGGAGCTAAATGCCTCAGGCCGGTGCACTCTGCCCATTG GTATGGCCAGCCTCTGCAAGGACAGCAGAGGGGTAGAGGAGAGATGGGAAAGTGAACCCAGCTAGTCAGG GAGAAGGGCAAGACTCATGAACACATTGGTGGGCAGGAAATAGCCATGGGCTGAGGTGCCCATGTCCAGG GTCCAATGCATGATGGAGCTGGGACTGGGGGTAGGGGGAAAGCAGAGCTCTCCAGCTCTGCCCCTTGGGC AATTCCTTCCCCTTTGTGGGCCTCCGTTTCTCACTCCATAAAAAGAGAAAGATTAGCCCTACCCAGAGGC ACCTGCTTGCCCCAAGGGATGCTGGGAGGCCTGGATGGTAATTGAGGACCTCTTGGAAGAAGTGGCACTG AGGATATGGATGGGGCCCCCTGCAGATGAGTCCAACACCATCCACTTGAAGGTGATTGAGCAGCGGCCAG ACCCTCCGGTGGCCCAGGAGTATGATGTACCTGTCTTTACCAAAGACAAGGAGGATTTCTTCAACTCACA GTGGGACCTCACTACACAACAAATCCTGCCCTACATTGATGGGTTCCGCCACATCCAGAAGATTTCAGCA GAGGCAGATGTGGAGCTCAACCTGGTGCGCATTGCTATCCAGAACCTGCTGTGAGTGGGCCTACAGTCAT ACCTGGGACAAGGTCACCAGCCAGGGAAGACCTCAGGGGCCCTGGGTGTGAGGGTTGGGAAGGCATGGTC CTCAGAAGCTGAGCACAGCTCTCTCTCTCAGGTACTACGGCGTTGTGACACTGGTGTCCATCCTCCAGGT AGTACTCCAATGTATACTGCCCAACGCCCAAGGTCCAGGACCTGGTAGATGACAAGTCCCTGCAAGAGGC ATGTCTATCCTACGTGACCAAGCAAGGGCACAAGAGGGCCAGTCTCCGGGATGTGTTCCAGCTATACTGC AGCCTGAGCCCTGGCACTACCGTGCGAGACCTCATTGGCCGCCACCCCCAGCAGCTGCAGCATGTTGATG AACGGTCAGAGGAGAATTTGCTGGGGCATTTGGGAGTTACCTGAGGGAAGCTAGACCCTTTATGTCTCTC AGGAGCCCTGGATCATGGGGCACTGCCAATCCAAGCAGGCTTCCTGGAGATGATGGGCTACAGAGACAAA ATTGAAGGGAGACTACAGGAAAGGGTTGGCCTGCCTGAAAGAAGGCCTGGCCAGGGCGTCACCCCGTCCT CTGATCCTCACCCTAGGAAGCTGATCCAGTTCGGGCTTATGAAGAACCTCATCAGGCGACTACAGAAGTA TCCTGTGCGGGTGACTCGGGAAGAGCAGAGCCACCCTGCCCGGCTTTATACAGGCTGCCACAGCTATGAC GAGATCTGCTGCAAGACAGGCATGAGCTACCATGAGCTGGATGAGCGGCTTGAAAATGACCCCAACATCA TCATCTGCTGGAAGTGAGGCTGGTAGTGACTGGATGGACACATTGCTGTGGGTAGTCCCTCCTACTAGGA GGCTTGTCATACTGTCTAGAGGTTGACTCTTAGTTCTGTAAATAAAGACATCCATTTCAAAC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK098161 unedited GGGTCAACATTTGTATACGACTCATATAGGCGGCCGCGAATTCGGCACGAGGGGCAAGCT TGCGTGCCCCAGCGACACAGGCCTCTGCGCGAGGAACGAGGAGCTACGGGCCTGGGCCCG GTTATTGCCATGGGCAGCGGCTGCCGCATCGAATGCATATTCTTCAGCGAGTTCCACCCC ACGCTGGGACCCAAGATCACCTATCAGGTGCCACCCGGGCTCGCGGGACTGGGCGGGAAG AGGGACGTTCTCGAGAGCTCAACTGGCTGCCTGAAGGCAGTAGTTTCCCGTGCTGCACGC AGGCATGCTGCTTCATTCGCAGACAACTGTGAGGCTAGAGGATAGTCAGACCCAGGCCCC CGGCTCCCAATGTGGCAGGGAAGACATACGTCACATTGGCAGTGTTTGTAGGATATCGGG CTGTAGGGATCCGGTTGGGGGGTTCCAGGTCCCACAGAAGGTGAGTTGGGTGACCCATGA GTATCCCTGAACTGAGTGAAGAGTAAATGATGCATAGGAAGTTTAGGTAAGGCAGGAGAA AGTGTTCCAGGTAGAGGGAACAGCATATGCAGAAGCCAGGAAGGAGGTGAGATCATAGCG TATGTTTGGAAACAGAAAGAAGTAATAGGCCTGAGTTGGATGGGAAGAGAAGGGAACGCT GGAGAAAGAGACTTGGGCTTGNGGGGCATGAGGAGGTCACCATTGGTACTACACCCAGTA CAGATGTCCACTGAGGNGCCCATTGCCACTCCCCTNCCCAGGTCCCCTGAGACTTCATCT NCCGAGAGCTGTTTGCACAGTCCAAGTGTACATCATCACCAAGCCAGAGCTGCAGAACAA GCTTATCACTGTCTATGGAAAGAAGCTGATCGGCTGTCCTGTGTGCATCGACACAAGAAG TCAGNCGCATGCTCTCTCCTCACCNTGGCTTCNGTGTGA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK098161 unedited TTTTTTTTTTTTTGTTTGAAATGGATGTCTTTATTTACAGAACTAAGAGTCAACCTCTAG ACAGTATGAAAGCCTCCTAGTAGGAGGGACTACCCACAGCAATGTGTCCATCCAGTCACT ACCAGCCTCACTTCCAGCAGATGATGATGTTGGGGTCATTTTCAAGCCGCTCATCCAGCT CATGGTAGCTCATGCCTGTCTTGCAGCAGATCTCGTCATAGCTGTGGCAGCCTGTATAAA GCCGGGCAGGGTGGCTCTGCTCTTCCCGAGTTACCCGCACAGGATACTTCTGTAGCCGCC TGATGAGGTTCTTCATACGCCCGAACTGCCTCAGTTTCCTACGGTGAGGACCATAT
Restriction Sites	NotI-NotI
ACCN	AK098161
Insert Size	3420 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK098161.1
RefSeq Size	2512 bp
RefSeq ORF	2512 bp
Locus ID	10641
Protein Families	Druggable Genome
Gene Summary	As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.[UniProtKB/Swiss-Prot Function]

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