p35 (CDK5R1) (BC035448) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CDK5P35; CDK5R; NCK5A; p23; p25; p35; p35nck5a
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC035448, the custom clone sequence may differ by one or more nucleotides GCACCTGTCCCCCACGGACCCCGTGCTCTGGCTGCGCAGCGTGGACCGCTCGCTGCTTCTGCAGGGCTGG CAGGACCAGGGCTTCATCACGCCGGCCAACGTGGTCTTCCTCTACATGCTCTGCAGGGATGTTATCTCCT CCGAGGTGGGCTCGGATCACGAGCTCCAGGCCGTCCTGCTGACATGCCTGTACCTCTCCTACTCCTACAT GGGCAACGAGATCTCCTACCCGCTCAAGCCCTTCCTGGTGGAGAGCTGCAAGGAGGCCTTTTGGGACCGT TGCCTCTCTGTCATCAACCTCATGAGCTCAAAGATGCTGCAGATAAATGCCGACCCACACTACTTCACAC AGGTCTTCTCCGACCTGAAGAACGAGAGCGGCCAGGAGGACAAGAAGCGGCTCCTCCTAGGCCTGGATCG GTGAGCACTGTAGCCTGCGTCATGGCTCAAGGATTCAATGCATTTTTAAGAATTTATTATTAAATCAGTT TTGTGTACAGTATGTGTCTAGCAAAGCCACCAAGGGCCTCACCTTTCCCACAGTCTCTCCCTGGGGTTTT TTTCATCCCTGCCAAGAACTCTGGGCACTTTTGAACTCACGAGCCTTGCGCAAAACCCAGAAGATGTATT CAGAGCCACCCAGGCCACTGACCTCCCACTTTGGGGAACTCAAAGGACTGACCTGCCCCTGCCGCCTGTG CCCTTGCTGGGTCCAGGGTAGGCAAGGCTGCCGGCTGCACCCTGTATGGAGCTAGAGAGGGGCCTCTGGC TGCCTGGCCCAGGGAGGAATTGGGGTTTCTGGTTGGAGGCCCTTTTCTGGCTCCTGTGTGGAGTTATTCA CTCTCCCAGAGGCTCCTGGAGCCAGCCACCCTAACTGAGCTGCCAGTGGGGTCGTGAGGCAAGATCCCCG CCACCCGGGGACATCTTCAATCTAGGCGAGGCGAAGCTGAGCGGGTCTAGTGGAAAGATTGTGTCTGGTC GTTTGACCACACACCGCCCTGATTTGCTGTTTTCTTTTTTTAGGGAGAAGGGCTTTTCTTTAGTGGAGAA ATGGAACTCGCCCCCCTACCCCCTTGTCTGCTGCTCCCAGCCACGTTGGTGGTATTGGCCGATGAGCTGG TTTGACTCATTAATTTCTCTCAATTTGGGTCCCAGCTAAAGAGGTGGGGTGAAGCTGGGGACAGCTTTCC TGGGTGAGTTTTTCTTTTGAATAATGTAGTGCAGTCACCCTGTGGCAAATGCCAGGACAGCTGCAGGTGC CCATTAGCAACGCCTGCCTTCTGGGCAAGGTGAGGGATGCCATACCTTGAGACCAAGCCGGTGCCCACCT AAGGGCTAAGGCCTCTTGCTGGTGCACATGACATTTGTCCTGCAGAGCTGGGGGGAAGGCGATGTGGTGA CCCCACCCCCACCCGTTAATTTAAAGCTGTTTCTAAACAGTTGAGTTTCTTCTAAAGAGGAAGCCTTGCC CAGCAAGGACCAGTGAGACAGCCGGATCTTGGAGACAATTACAAGACGGGGAGTGAGACTCCTGATTGCT CTGGAAGCCTGCTGATTTCTACAACTGATCATTTGCAGCTGCTGGTTTTGGTTTCCACCTTACCCTACTG GCTGTAAAAACACAAATGTGTACTTTATTGATTTTCTTTCTAATTCTCCCGCATTGGTGGCTTGGGACTT GGGAGAGGGAGCAAGCCTTCCTCCATGGCCCATCACTCGGCTGTGGAGAACAAAGACCAATGTGAAGACA CTACAGAGGATTCTGTCTTCCAGGCCCAGTCCACTGGGGAGTGCTGGAATGGGGACCTGGGGTGGGGAGG CAGAGGGTCACTTTACATAGGATTAAGTTCGAGGTGGCTACCGATTTCAGCACATGCACTACTGAAATTT ACACAAAAAGAAAGCTGTGAAATTGAAGTCCCAATTTAAGAGTCCTGAGGCAGAACCTGGTGGCTGGAGG CATTCCCAGAGGTGGGGAAGAGAGCCTGCCCGGCCGGAGAACATCTGCCTTGCTGCACCTGAGGCCCAGC AGAGCCGTTCCTGGGACTGTCAGATAATCGGTGCAGCGGTGGAAGGAGCCTGCGGCTGCTGGCACAGACT TCACACAGCACCTCCTCTCTGCTGGGTTTCCACACAGCCTGTCTTCAGATCCTGCTGCCGCGTGCGACCA GAGGTGGGAGGCCCCTGGTGGCATGGAAGAGGGAGGGTCAGTGCCAAGTCTCAGGAGGAGGGCGCATGTG TGTATCACCCTCAGCTGGCGGAACCTGGCTGCGAACTGTGCAGTTACGTTGCATCCACAGGATTCCAGTT GCGTGTCTGTTTCCTTCTCTTTCTCCGTATTTATTTTTTTATTCTTCGGAGGAGGTGGACATTTCGGAAG TGGTGGGGACTAAGGGAAGAACTCTCTAGTTCCCTCAGTGTGAAGCCTGTCGTGTTCTCTCCCCTTGCAC TGGTCATCAGTATTGTGTAAAGGAACAACTGATATACTTGAGTGTGCAAGCAAAGAACCCATTTGCCATG CTGCTATGAAGACTACTTTTAGATCAACAATAAAAAAAAACCTACAAAAAAACCTTTATTCTTTAATTGT TGCTTTTACGGTGATATTGTGCATGCAAACCAGGAGCATTTTGTGTCTTAAGAAAAATAATCTTAGAACA GATGGCTGTGAAAATTACACCCATGCACAGAACAAGCCACAGGAATAATAGTTCAGGATTTGGTTTTTCT CTTTTTCTTGTAAACCTGGAGGGTTGATATATTCTTTCCATGCAGTTATTAGAACTTAGTTTTGTTCCAA CAGTTAAACTTGCAATGAAAAGAAAATGTGCCATTTTTTTCACTCAGAATTATTCATAGCTGTATATTTG AAACTGCTAATTACACACGTGTGATGTATGTTGGTTTTTTAGTGCAATTTCTTCTGTAGCTATTCTTTGA CCAAACTGTGGGTATTGTTAATATTAATTTATATTTGTCTCATTTTGTATGTATGTGTAGTGTGTTTGTG AGTATGTGTGGTTTATAATCTGACAAAGTCATGAAGCTCAGTTTGGCTGTAATTTAATTCCCCTTCCCTT ATTTTTATTTATTTTTGTACTGTGCTGATTCAATAAAATGCACTGACCATCCATAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC035448 unedited GGTGCACCATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGC CTGCACCCCCGGCCAGCCAGCTCTCGGGTTCCCAGACCGGGGGCTCCTCCTCAGTCAAGA AAGCCCCTCACCCTGCCGTCACCTCCGCAGGGACGCCCAAACGGGTCATCGTCCAGGCGT CCACCAGTGAGCTGCTTCGCTGCCTGGGTGAGTTTCTCTGCCGCCGGTGCTACCGCCTGA AGCACCTGTCCCCCACGGACCCCGTGCTCTGGCTGCGCAGCGTGGACCGCTCGCTGCTTC TGCAGGGCTGGCAGGACCAGGGCTTCATCACGCCGGCCAACGTGGTCTTCCTCTACATGC TCTGCAGGGATGTTATCTCCTCCGAGGTGGGCTCGGATCACGAGCTCCAGGCCGTCCTGC TGACATGCCTGTACCTCTCCTACTCCTACATGGGCAACGAGATCTCCTACCCGCTCAAGC CCTTCCTGGTGGAGAGCTGCAAGGAGGCCTTTTGGGACCGTTGCCTCTCTGTCATCAACC TCATGAGCTCAAAGATGCTGCAGATAAATGCCGACCCACACTACTTCACACAGGTCTTCT CCGACCTGAAGAACGAGAGCGGCCAGGAGGACAAGAAGCGGCTCCTCCTANGCCTGGATC GGTGAGCACTGTAGCCTGCGTCATGGCTCAAGGATTCAATGCATNTTTAAGAATNTATTA TTAAATCAGTTTTGTGTACAGTATGTGTCTAGCAAAGCCACCAAAGGCCTCACCTTNTCC ACAGTCTCTCCCTGGGGTTTTTTTCATTCCTGCCAAGAACTCTGGGCACTTTTGAACTCA CGAGCCTTGCGCAAAAACCAGAAGATGTATNCAGAAGCCACCAGGCCACTGACCTCCCAC TTTGGGGACTCAAAGACCTGACTGCC
Restriction Sites	NotI-NotI
ACCN	BC035448
Insert Size	3800 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC035448.1
RefSeq Size	3149 bp
RefSeq ORF	3149 bp
Locus ID	8851
Protein Families	Druggable Genome
Protein Pathways	Alzheimer's disease
Gene Summary	The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease. [provided by RefSeq, Jul 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.