FHDC1 (AK098403) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK098403, the custom clone sequence may differ by one or more nucleotides GAATTGTGCCACCTGGGCTGCCCGATGCCCCAGGGATTCCCCAGTGCTGGATGCCTACGGTCCGATCTCA GGGCACAGAGCTCACAGACAATAGACGCTGCTTTGCGGGGCAGGGAGGACCAGGCGCTGTCTGCAAGGCT GCCTGACCCCCAAGTCCACTATGTGCCCTAGACAAGGCAGGGTGCCCCCACCCCGATCCATCACCCTCCC AGCAGTGTGGGCTGGGCAGGGCAGGGCGGGGTGGGGCGGGGCAGGCCAGCCACAACCTCCCAGTGAGGGG CGGACATCACCACTGAGGCTGGGGATTCAAGACAGGCCTGAACCGGGCACACACCAGTTTGGTGGCTCCC CCACCAGGCCAGGCCCACACCCAGGGACACAGCGTGGCAGAGCGTCCAGCCCAGCCCTGGCATGGGGGCA TCCAGAGAAGGCAGGTTAGGCTCACGGCTCCCGCAGGGCTGCAGATGGACACCCATGGGCCCTGCAGAGC AGCTGGCCATCCCCTGGGCCAGACAGTGGTTCACCTGCTGTGGGCTCAGCCACCTGTCCCCCGAGGGGTG CCGCGCCCTCCCCCATATCCCCGGGGCCACAGCTGATGCACAAATACGACTCAAGACAAGAAGTTGAAAT GGAACAAGATGAGCACGGATGAGAGGGATGCCTGAGCCTTTCTCTTTGGAGTCCCCACCCCCGGAGGGGA CAGCACAGGACAGGATGGGGGATGTCAGGCACCTCCAAGGGTGACAGCACAGATGGCACGTGGACATGAA GACGCAGCAGGGACGGGACTGTGCTCTGGCAAAGCTGTGCTTGGACTCTGGAGCCATTACCTGGAGCAGA ATCACCTCTGGCACAGCTGGGTCCACAAGAGCCCGGGCAGCCGGGAACAACCCTGGGGACGGGCAGGCAG ACTCCGTCCAGCTACCCGGGAAGAGGCACAGGCAGCCGGAAGCAAACCCTGGGGTGGGCAGGCAGGGTTC CGTCCAGCTGCCCAGGAAGAGGCACAGGGCAGAGGCAGCTCCCTAAGCTTTCCTGAGGACTTTGTGTTTT CTAAGAACTTGAAACACTACAGACTGAGTTTTTAAGAGAATAAAGTACGTGGAAGAGACTGACAGGTCTG CGGAAGGCTCCCAGACTCCCAGATGGCGTCTAAGCATTGGCACCCTGCTGAAAGGATCTTTTGATCTACT CTCAACGTGCTGGAGCCTCAGGGTCTGCTGGATCCCCGGCTTTTTTTCAGCTTTGATCGGTGCAGCTTTG CCCTTCCTGGGGCCTGGGTAGGGGCTGCCTAGGGGCCCGCTGGTGAGGATTCCACCTCCATCACTGATGT CTGCCCATGCCCTGTCTGCCTGCCAGGCTAGACCCACTCCTAAGGAAGTGCTCTGTGGCCCGAGTTCCTC CGCAGGCCCAGGGTCCGGAGGGAGCTCTGGGGAGATTAGAAATGGGGCTGGCTGGGCATAGTGGCTCCTG CCTAGAATCCCAGCACTCTGAGAGGCCAAAGCAGGAGGAACCACTTGAGCCCTGGAGTTCAAGACCAGCC TGGGCAGCAAAGCGTACCCGTCTCTACAAAAAAAAAAAAAAAAAATTAGGCAGGTGCGGGAGAGTGGTGC ATGCCTGTGGTCCCACCTCAGCCTCCTGAGAAGCTGGGACTACAGACGCGTGCCACCATGCCTGGCTAAT TTTTAACTTTTTTTTTTGTCAAGATGGGGGTCTCCCTAGGTTGCCCAGGCTGATCTCGAACCCCTGGGCT CAAGAGATCCTCTCGCCTCAGCCTCCCTAAGTGCTGGGATTACAGGTGTGGGCCACGGCGCCCGGCCAAG CGGCAGCTTCACCAATGACTTGAATGTGAAGCAGAAGGATTCCGAGAAGAAACGGAGCCGTCACAAATGC ATTTCGACCAGTTGGGTGACTCCAGACCCAGAGCCGGGCGGACAGCACCTGTTTTCTGTCTTCTCGCGTC AGCTGTTTCTTCGTGTCTTCAGAAGGTGGGCCCTTCTGGAATCGGGTCCAGCGTGGGCGCTTGGGCCTCC GCTGACGAGTATGGACAGCGGCCAGGGACGCTCGCCCCTCCACTCTCATTCCAAGGCCCCCTTCCCAGGG CCCAAAGCTGGGGCATGACAGGGTGGCAATGGGATTGGGGAGTCTCTTGGCCAACTGCAAGTGGCTTTCG ACACCCCCAATCCCAGCTGAGTTGTCAGACCAGGCAGACTGCGTGGCCGGGAAACTGGCCCCTGGCACTG TGGAGTGGTGCTGGGGCTGAGCACCACTGCCCCACCGGCACTGCTACCCAGGGTCTCTGCCCTCCAAGGG ACGTGGTCCCTGACAGAGGCTGTGCCAGCCTCATCTTTGAGCCTTCACCCCTTCCAAGCCTCACCAGAAT TTGTTGCCGAAAATCAACAACAGAAAGGCAGTGAAAGAAGACAGATCCATGATCTGCCTGTGGCCTGGCT GCCCGTGGGCCAGGATGCCTGCTGTGACCACCAGGGAGACACAGGGACCAACGTCCTTTGTGAAGGATGC AGGCACTGGCTTTGCAGAGCCGCCCTCCTAGCAACTTGGGGATGGAAGGGTGGTGGGAAGCAGGACCCGC CCAGACACAGGTGTAGGCGATTGGAAGGTGTGGGCAAGGGCCCCCTATGAGGGCAGAGGGCCTGCTCCTG GACACCCTGGCACATCCCAAATATACACCAGGCACCAGGGGAGTGGCCCACTCAACCCGTGAGGAAATGG ACTCAGACCCGGACTCCAGAGTCCCACTGGAATTGGACCCATAATGGCCCTGTGGTCCCCGGAGTGAGCG TCAGCTCAAACCTGGACCCAGCAGCCCTCCAGGTCTGCAGATCCACACTAGGGATGGAGCCAGTGTCTTG GGTCCACGTTGTGGGGCTTGGGGGACAGAAGAGGCAAGGTCAGACTAACAGCACAGCCCGGTCCCCAGTG GGCCCAACACCTGGCTCCAGTGCCTATGGAGCTCCCCAGGGTTCTCCCCAGGACCCTGACCTCCAGTCCC CACAAGGCTGTCAGCCCCAAAGGCACACACACCCGAAGGAGCCCAAGCTGGCGCTGCGAGTCAGGCGGGA AAGGCCCGGGCTGCAGCTGCTTGCCGGGGTCACCCTTTGACTATAATTGAAGCGCCCAGCAGGGCAGGAG AGGACCACACAGAAGGTCAGGGCCAGAGCCACACCCAGCAGTGAGAGGAGCCCCAGGCACAGCCAGCCAG AGCCCACAAGCTTCCGGGAGGGGAGAAAGATACCATGAACCCAAGGAGGGGACCCAGTAGAGAAGGCCCA GCACCCAGAGTGAGAGGCAGGCTCAGTGGACCCCTGGCCCCGCCATGCAGCCGGGCCCTCCCCACCCAGG CTGCGTGACTGAGGCCAGAGGGCCCGTGGAGAACCACTGGAGAGGCCAGTGACTGCACGAGAACGCCAGG CAACTTACTAACTCTAGTCAGGCGGCTCAGAATCTGGCCACTTCCACATCGGCGCTGCAAGGAAACACAA GGCTCAGTTGGAGGCAGCATGGGGTCTGAGGACTCGGGGGTGCAGAGGAGGGGGGACCACGGGCGAGCTG CCAGGAGACCTAGAACCCTGGAGCACTCGGAGTCGGCACACAGCTGGGGCTCTAGCTCTCCCTGGACGGC GCCTGCGGAGCCTGCCTCACTTGCGGGCCTGGAGGGAGCCAGGAGCCTGCCTCAGCTGCAGACGGCCCCC CGCAGAGCCCGGGCAGAGTGAGTGACTGTCACTGGGGCCAGCACCGTGTGCAAGCCCAGGTTATCTAGCA GGCGACTGAGTGCCGAGAAAATACCTGGCAGAGGTGGGCACAAGGCGGGGTCCCCACGGCAAAGACGCAC GCCGGGAACCGGAAGGAGGTCCATACCCAACTCGTGCAATAAATAAAACTTTTATTCAAACAAGTAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK098403 unedited AGGCGGCCCGCGAATTCGCACGAGGCACCAATGACTTGAATGTGAAGCAGAAGGATTCCG AGAAGAAAACGGAGCCGTCACAAATGCATTTCGACCAGTTGGGTGACTCCAGACCCAGAG CCGGGCGGACAGCACCTGTTTTCTGTCTTCTCGCGTCAGCTGTTTCTTCGTGTCTTCAGA AGGTGGGCCCTTCTGGAATCGGGTCCAGCGTGGGCGCTTGGGCCTCCGCTGACGAGTATG GACAGCGGCCAGGGACGCTCGCCCCTCCACTCTCATTCCAAGGCCCCCTTCCCAGGGCCC AAAGCTGGGGCATGACAGGGTGGCATGGATTTGGNGGGGAGATTCTCTTGGCCAACTGCA AGTGGCTTTCGACACCCCCAATCCCAGCTGAGTTGTCAGACCAGGCAGACTGCGTGGCCG GGAAACTGGCCCCTGGCCTGTGGNAGTGGTTGCTGGNGGCTGAGCACCACTGCCCCACCG GCACTGCTACCCAGGGTCTCTGCCCTCCAGGGNACGTGGTCCCTGACAGAGGCTGTGCCA GCCTCATCTTTGAGCCTTCACCCCTTCCAAGCCTCACCAGGTAAGGGGCAGTTCAGCCCC CTCAGCTCAAAGGGACCTGNGGGATGNNNNNGGGGTCTTTAAAGGCCTTCCCAGAAACAG CGTCTCGGGAAGGGAGGAAGAATGGGAGCGGAAGGCANGGTGGTGGCCAGGGCAACCCAG AGCATCTCCATCCAGTCCCCAAAGTTCAGGAGCATTTTTCTACCTAATCTTCCAGAATTT GTTGCCGAAAATCAACACAGAAAGGCAGTGAAAGAAGACAGATCCATGATCTGCCTGTGG CCTGGCTGGCCGTGGGCCCAGATGCCTGCTA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK098403 unedited NCCGATCTTATGTACGCGCCGCATTCTANGATCGGTTTTTTTTTTTTTTTTTTGAATAAA AGTTTTATTTATTGCACGAGTTGGGTATGGACCTCCTTCCGGTTCCCGGCATGCGTCTTT GCCGTGGGGGCCCCGCCTTGTGCCCACCTCTGCCAGGTATTTTCTCGGCACTCAGTCGCC TGCTAGATAACCTGGGCTTGCACACGGTGCTGGCCCCAGTGACAGTCACTCACTCTGCCC GGGCTCTGCGGGGGGCCGTCTGCAGCTGAGGCAGGCTCCTGGCTCCCTCCAGGCCCGCAA GTGAGGCAGGCTCCGCAGGCGCCGTCCAGGGAGAGCCAGAGCCCCAGCTGTGTGCCGACT CCGAGTGCTCCAGGGTTCTAGGTCTCCTGGCAGCTCGCCCGTGGTCCCCCCTCCTCTGCA CCCCCGAGTCCTCAGACCCCATGCTGCCTCCAACTGAGCCTTGTGTTTCCTTGCAGCGCC GATGTGGAAGTGGCCAGATTCTGAGCCGCCTGACTAGAGTTAGTAAGTTGCCTGGCGTTC TCGTGCAGTCACTGGCCTCTCCAGTGGTTCTCCACGGGCCCTCTGGCCTCAGTCACGCAG CCTGGGTGGGGAGGGCCCGGCTGCATGGCGGGGCCAGGGGTCCACTGAGCCTGCCTCTCA CTCTGGGTGCTGGGCCTTCTCTACTGGGTCCCCTCCTTGGGTTCATGGTATCTTTCTCCC CTCCCGGAAGCTTGTGGGCTCTGGCTGGCTGTGCCTGGGGCTCCTCTCACTGCTGGGTGT GGCTCTGGCCCTGACCTTCTGTGTGGTCCTCTCCTGCCCTGCTGGGCGCTTCATTATAGT CAAAGGTGACCCCGGCAGCAGCTGCAGCCCGGGCCTTTCCCC
Restriction Sites	NotI-NotI
ACCN	AK098403
Insert Size	2500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK098403.1
RefSeq Size	3948 bp
RefSeq ORF	3948 bp
Locus ID	85462
Gene Summary	Microtubule-associated formin which regulates both actin and microtubule dynamics. Induces microtubule acetylation and stabilization and actin stress fiber formation (PubMed:18815276). Regulates Golgi ribbon formation (PubMed:26564798). Required for normal cilia assembly. Early in cilia assembly, may assist in the maturation and positioning of the centrosome/basal body, and once cilia assembly has initiated, may also promote cilia elongation by inhibiting disassembly (PubMed:29742020).[UniProtKB/Swiss-Prot Function]

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