BTBD7 (AK074354) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FUP1
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK074354, the custom clone sequence may differ by one or more nucleotides AACGTTTTGGGGATGAAAGTCCACTCTTGACAATGAGACAGCCTGGGAGATGTCGCGTAAACAGTACACC TCCTGCAGAAACCATGTTTACAGATCTGGACTCTTTTGTGGCCTTCCATCCACCCTTGCCCCCTCCACCA CCTCCCTACCACCCCCCAGCTACCCCAATCCATAACCAACTCAAGGCAGGCTGGAAGCAAAGACCTCCCA GTCAGCACCCTTCACGTTCATTTTCTTATCCCTGTAATCATTCGCTGTTCCACTCCAGAACAGCTCCTAA AGCTGGCCCTCCCCCAGTCTACTTGCCGAGTGTGAAAGCTGCACCGCCTGATTGTACCAGCACTGCAGGA CTGGGCAGACAGACGGTGGCTGCTGCTGCCGCCACCACCACCTCAACAGCAACAGCAGCAGCAGCAGCAG CATCTGAGAAGCAAGTGCGAACACAACCTGTGCTGAATGATCTGATGCCAGACATCGCGGTGGGTGTGTC CACACTGTCACTCAAGGACAGGAGGCTTCCAGAGCTTGCTGTAGACACAGAATTAAGCCAGTCAGTTTTT GAAGCAGGACCAGGGCCTCCCCAGCATCTGTCGTGTATTCCACAGAGACATACACACACTTCTCGGAAAA AACACACACTAGAGCAAAAAACAGACACCAGAGAAAATCCACAGGAATATCCGGATTTCTATGACTTCTC AAATGCTGCTTGCAGACCTTCTACTCCTGCTCTCAGCAGACGCACCCCTTCCCCTTCGCAAGGTGGATAT TTTGGTCCCGATCTGTACAGCCACAATAAGGCATCACCAAGTGGCTTAAAGTCAGCCTACCTACCTGGTC AGGCGTCTCCTAAAAAACAGGAAGAAGCTAGGAGAGAATATCCACTTTCCCCTGACGGGCATCTACACAG ACAAAAGAATGAGCCGATACACCTGGATGTCGTTGAGCAACCTCCCCAGCGGTCAGACTTTCCTTTGGCA GCCCCAGAAAATGCTAGTACCGGTCCAGCCCATGTCAGGGGACGAACTGCAGTAGAAACTGACTTGACTT TTGGGCTGACTCCTAACAGACCTTCACTTTCTGCATGTAGCTCTGAAGCTCCCGAAGAGAGATCCGGTAG AAGACTGGCAGACAGTGAGTCCCTGGGCCATGGAGCTCAGAGAAATACAGATTTGGAAAGGGAAGATTCA ATAAGCAGAGGAAGGAGGTCACCAAGCAAGCCGGACTTCCTCTACAAAAAGTCTGCCCTCTGAGAGCAAC CTCCAAGTCGTCTGTGCCTGAGATGTGAAACATCCCATTTTATGATGTAACCCAACAACTTACAGACCAG TTCATCAATGCCAGCTGATAACTCGGTTTCACATGATTTTGTTCTAGTTTTTGTGTATATATGTGTTTAT TAGACATGGCATGCTAAGAAGGTTTTGTTTTTTTTTTTTAATGTTGCATCTGTCTAATTTGTCTTTGAAC AATTATGTCGGGAGGCATTTTTAAGAACAAGCAGGCTGGCACTTTTTATTTCTCTTTACAAATGATGGAA TTTTTTTGCACTTGTACATTTATTTTCTCTGTATTGATTTTATATCAGTATGTTAAACTTTATTGCCACA TTTAAAGGACTGTATTTTCATACTTTTTTGCATTTTACCTTTCATCTACCAATCCATGTGCATTGGATTG CACACTAAGATGTATTTTCTTACGAAATAGTTCTGTGTTTATTTTTTAATTATAGAAATTCCAAAGAACA GCACATCAGAATGCTCCTCTCTTTTCAGTAATTGTTTTAGTTCAGAGATCTTCCCGCTCAGCCTCCATAA ATCTAATGTCATTTTTTTTTTTAATAAGTCCTAGGAGCTGGGCTTCATTCCTCCAGCGGGAGTCCAGTCA GGGTCCTGAAGTTTGCTGGTTCAGGAAAGAACAGGAAAGGGAAAAGCCTTCTGTGCCCAGGCTAAAGCTG TTACATTTCCACAGTGTGGATGCAGTCAGAATCCCTGCACATTCTGGTCATAAGGATTAGAGTCACCAGA TGAGGCTCTAAACAGATCGCATCCCTTGGAATTAGAAAGCAGTGGAGACCGGGCGCAGTGACTCACACCT GTGATCGGCACTTCGGAAGGCCGAGGCGGGCAGATCACTGAAGCTCAGGAGTTCAAGACCAGCCTGGGCA ACATGGCGAAACTCCTTTCTATAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK074354 unedited GCGTTGCANATTTTGTAATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGTTTG GTCTTGCAGATGCTGCTGCAGAGCTGTTGCAGAATCCTCACAAATTCTTTCCTGATGAAC GTTTTGGGGATGAAAGTCCACTCTTGACAATGAGACAGCCTGGGAGATGTCGCGTAAACA GTACACCTCCTGCAGAAACCATGTTTACAGATCTGGACTCTTTTGTGGCCTTCCATCCAC CCTTGCCCCCTCCACCACCTCCCTACCACCCCCCAGCTACCCCAATCCATAACCAACTCA AAGCAGGCTGGAAGCAAAGACCTCCCAGTCAGCACCCTTCACGTTCATTTTCTTATCCCT GTAATCATTCGCTGTTCCACTCCAGAACAGCTCCTAAAGCTGGCCCTCCCCCAGTCTACT TGCCGAGTGTGAAAGCTGCACCGCCTGATTGTACCAGCACTGCAGGACTGGGCAGACAGA CGGTGGCTGCTGCTGCCGCCACCACCACCTCAACAGCAACAGCAGCAGCAGCAGCAGCAT CTGAGAAGCAAGTGCGAACACAACCTGTGCTGAATGATCTGATGCCAGACATCGCGGTGG GTGTGTCCACACTGTCACTCAAGGACAGGAGGCTTCCAGAGCTTGCTGTAGACACAGAAT TAAGCCAGTCAGTTTCTGAAGCATGACCAGGGCCTNCCCAGCATCTGTCGTGTATTCCAC AGAGACATACACACACTTCTCGGAAAAAACACACACTAGTAGCAAAAAACAGACACCAGA GTAAAATCCCAGGNAATATCCGGNATTTCTATGACTTCTCAAATGCTTGCTTGCAGACCT TCTACTTCTGCTCTTCAGCAGACGCACCCCTTTCCTTTGCAAGGTGGATATTTTGGTCCC GATCTGTTCAGCCACATTAGG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK074354 unedited CGAAATACTATGNNACCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTGTA TTTTTTATAGAAATGGAGTTTCGCCATGTTGCCCAGGCTGGTCTTGAACTCCTGAGCTTC AGTGATCTGCCCGCCTCGGCCTTCCGAAGTGCCGATCACAGGTGTGAGTCACTGCGCCCG GTCTCCACTGCTTTCTAATTCCAAGGGATGCGATCTGTTTAGAGCCTCATCTGGTGACTC TAATCCTTATGACCAGAATGTGCAGGGATTCTGACTGCATCCACACTGTGGAAATGTAAC AGCTTTAGCCTGGGCACAGAAGGCTTTTCTCTTTCCTGTTCTTTCCTGAACCAGCAAACT TCAGGACCCTGACTGGACTCCCGCTGGAGGAATGAAGCCCAGCTCCTAGGACTTATTAAA AAAAAAATGACATTAGATTTATGGAGGCTGAGCGGGAAGATCTCTGAACTAAAACAATTA CTGAAAAGAGAGGAGCATTCTGATGTGCTGTTCTTTGGAATTTCTATAATTAAAAAATAA ACACAGAACTATTTCGTAAGAAAATACATCTTAGTGTGCAATCCAATGCACATGGATTGG TAGATGAAAGGTAAAATGCAAAAAAGTATGAAAATACAGTCCTTTAAATGTGGCAATAAA GTTTAACATACTGATATAAAATCAATACAGAGAAAATAAATGTACAAGTGCAAAAAAATT CCATCATTTGTAAAGAGAAATAAAAAGTGCCAGCCTGCTTGTTCTTAAAATGCCTTCCGA CATAATTGTTCAAAGACAAATTAGACAGATGCAACATTTAAAAAAAAAAACAAAACCTTT CTAACATGCCATGTGCTAATAACACCTATATACCCCAAAACTAGAACAAAATCTGTGAAA ACGAGTTATCAGCC
Restriction Sites	NotI-NotI
ACCN	AK074354
Insert Size	2500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK074354.1
RefSeq Size	2213 bp
RefSeq ORF	2213 bp
Locus ID	55727
Gene Summary	Acts as a mediator of epithelial dynamics and organ branching by promoting cleft progression. Induced following accumulation of fibronectin in forming clefts, leading to local expression of the cell-scattering SNAIL2 and suppression of E-cadherin levels, thereby altering cell morphology and reducing cell-cell adhesion. This stimulates cell separation at the base of forming clefts by local, dynamic intercellular gap formation and promotes cleft progression (By similarity).[UniProtKB/Swiss-Prot Function]

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